LOCUS       sequence-370416-        7911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human ELF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: MAC_N_ELF2
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(529..1320,1321..1389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1152..1171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1390..1494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1569..1588)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1760..2139
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2140..2343
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2294..2314
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2345..2363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2366..2384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2394..2418
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2586..2936,2937..2939,2940..3548)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3549..3575
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3606..3629
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             3666..3689
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    3702..3726
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5326..5350)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5388..5405)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5394..5618
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5751..5770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5902..5949
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5957..6979
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5957..6979
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7109..7230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7146..7165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7200..7219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7279..7295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7279..7295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7292..7314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7303..7319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7327..7333,7334..7351,7352..7357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7372..7393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7510..7527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(7681..7911,1..358))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7761..7780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
       61 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      181 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1501 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1561 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1621 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     1681 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     1741 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     1801 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     1861 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     1921 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     1981 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2041 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2101 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2161 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2221 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2281 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2341 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     2401 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2461 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
     2521 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
     2581 agaccatgaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt
     2641 tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc atcaacaagc
     2701 acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc aagggctaca
     2761 gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag ctggatggcg
     2821 gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc gaccggatcg
     2881 gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc agaggcggca
     2941 ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg ttctggagac
     3001 tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc gtgatggccg
     3061 aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac gacctgtatc
     3121 tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca ggcgacgccg
     3181 cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa gagagcgtgg
     3241 tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg aacaccctgg
     3301 ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag gaaggcctgg
     3361 ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc gtgaaactga
     3421 tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag ggggccctgc
     3481 tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc ctgcggagcg
     3541 ccgagaagta cccatacgat gttcctgact atgccggtac cgagctcgga tccaccatgg
     3601 ctagctggag ccacccgcag ttcgagaaag gtggaggttc cggaggtgga tcgggaggtg
     3661 gatcgtggag ccacccgcag ttcgaaaaag cggccgatat cacaagtttg tacaaaaaag
     3721 ttggcatggc gacgtctctg catgagggac ccacgaacca gctggatctg ctcatccggg
     3781 ccgtggaagc atcagttcac agcagtaatg cacactgtac agataagaca attgaagctg
     3841 ctgaagccct gcttcatatg gaatctccta cctgcttgag ggattcaaga agtcctgtgg
     3901 aagtgtttgt tcctccttgt gtatcaactc cagaattcat ccatgctgct atgaggccag
     3961 atgtcattac agaaactgta gtggaggtgt caactgaaga gtctgaaccc atggatacct
     4021 ctcctattcc aacatcacca gatagccatg aaccaatgaa aaagaaaaaa gttggccgta
     4081 aaccaaagac ccagcaatca ccaatttcca atgggtctcc tgagttaggt ataaagaaga
     4141 aaccaagaga aggaaaagga aacacaacct atttgtggga gtttctttta gatctacttc
     4201 aagataaaaa tacttgtccc aggtatatta aatggactca gagagaaaaa ggcatattca
     4261 agctggtgga ttcaaaggct gtctctaagc tttggggaaa gcataagaac aaaccagaca
     4321 tgaactatga aaccatggga cgagctttga gatactacta ccaaagggga attcttgcaa
     4381 aggttgaagg acagaggctt gtatatcagt tcaaggatat gccgaaaaac atagtggtca
     4441 tagatgatga caaaagtgaa acctgtaatg aagatttagc aggaactact gatgaaaaat
     4501 cattagaacg agtgtcactg tctgcagaaa gtctcctgaa agcagcatcc tctgttcgca
     4561 gtggaaaaaa ttcatcccct ataaactgct ccagagcaga gaagggtgta gctagagttg
     4621 tgaatatcac ttcccctggg cacgatgctt catccaggtc tcctactacc actgcatctg
     4681 tgtcagcaac agcagctcca aggacagttc gtgtggcaat gcaggtacct gttgtaatga
     4741 catcattggg tcagaaaatt tcaactgtgg cagttcagtc agttaatgca ggtgcaccat
     4801 taataaccag cactagtcca acaacagcga cctctccaaa ggtagtcatt cagacaatcc
     4861 ctactgtgat gccagcttct actgaaaatg gagacaaaat caccatgcag cctgccaaaa
     4921 ttattaccat cccagctaca cagcttgcac agtgtcaact gcagacaaag tcaaatctga
     4981 ctggatcagg aagcattaac attgttggaa ccccattggc tgtgagagca cttacccctg
     5041 tttcaatagc ccatggtaca cctgtaatga gactatcaat gcctactcag caggcatctg
     5101 gccagactcc tcctcgagtt atcagtgcag tcataaaggg gccagaggtt aaatcggaag
     5161 cagtggcaaa aaagcaagaa catgatgtga aaactttgca gctagtagaa gaaaaaccag
     5221 cagatggaaa taagacagtg acccacgtag tggttgtcag tgcgccttca gctattgccc
     5281 ttcctgtaac tatgaaaaca gaaggactag tgacatgtga gaaataccca actttcttgt
     5341 acaaagtggt gacgtaagct aggggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5401 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5461 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5521 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5581 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5641 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5701 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5761 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5821 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5881 gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc tagaaagtat
     5941 aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat
     6001 cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     6061 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     6121 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     6181 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     6241 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     6301 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     6361 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6421 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6481 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6541 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6601 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     6661 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     6721 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     6781 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     6841 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     6901 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     6961 tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg ccttctatga
     7021 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7081 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7141 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7201 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7261 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7321 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7381 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7441 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7501 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7561 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7621 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7681 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7741 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7801 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7861 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg t
//
