LOCUS       sequence-37042-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S
  TITLE     Tagging muscle cell lineages in development and tail regeneration 
            using Cre recombinase in transgenic Xenopus.
  JOURNAL   Nucleic Acids Res. 2003 Apr 15. 31(8):e44.
  PUBMED    12682379
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Apr 15. 31(8):e44."
COMMENT     Sequence Label: LCMV:ECFP(loxP)(FRT)EYFP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..130
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      172..181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(202..235)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    243..276
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    284..418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(308..327)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     362..381
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(577..598)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cnggngcagg cngggcgctg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
       61 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
      121 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
      181 cgatggatct cctaggcccg ggaagttcct atactttcta gagaatagga acttcggatc
      241 ctataacttc gtataatgta tgctatacga agttatgaat tcagatccag acatgataag
      301 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
      361 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
      421 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
      481 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag attacttgta
      541 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
      601 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
      661 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
      721 cacgctgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc cgttcttctg
      781 cttgtcggcg gtgatataga cgttgtggct gatgtagttg tactccagct tgtg
//
