LOCUS       sequence-370458-        8237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human SOX5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8237)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 8237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: MAC_N_SOX5
FEATURES             Location/Qualifiers
     source          1..8237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..497
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..701
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     652..672
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    703..721
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    724..742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     752..776
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(944..1294,1295..1297,1298..1906)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             1907..1933
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1964..1987
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2024..2047
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    2060..2084
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4010..4034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4072..4089)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4078..4302
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4435..4454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4586..4633
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4641..5663
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4641..5663
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5793..5914
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5830..5849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5884..5903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5963..5979)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5963..5979)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5976..5998)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5987..6003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6011..6017,6018..6035,6036..6041))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6056..6077
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6194..6211)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6365..6953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6445..6464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7124..7915,7916..7984))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7747..7766
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7985..8089)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8164..8183)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
       61 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      121 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      181 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      241 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      301 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      361 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      421 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      481 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      541 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      601 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      661 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccctatc agtgatagag
      721 atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt cagatcgcct
      781 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      841 ggactctagc gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt
      901 ggaattctgc agatatccag cacagtggcg gccgctcgag accatgaagg acaacaccgt
      961 gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg
     1021 cgagacactg ggcatgagca gagccgccat caacaagcac atccagaccc tgcgggactg
     1081 gggcgtggac gtgttcacag tgcccggcaa gggctacagc ctgcccgagc ccatccagct
     1141 gctgaacgcc aagcagatcc tgggccagct ggatggcgga tctgtggccg tgctgcccgt
     1201 gatcgacagc accaaccagt atctgctcga ccggatcggc gagctgaagt ccggcgacgc
     1261 ctgtatcgcc gagtaccagc aggccggcag aggcggcagg ggcagaaagt ggttcagccc
     1321 cttcggcgcc aacctgtacc tgagcatgtt ctggagactg gaacagggcc cagccgccgc
     1381 tattggcctg agcctggtca tcggcatcgt gatggccgag gtgctgcgga agctgggcgc
     1441 cgacaaagtg cgcgtgaagt ggcccaacga cctgtatctg caggaccgga agctggccgg
     1501 catcctggtg gaactgaccg gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg
     1561 catcaacatg gccatgcggc gggtggaaga gagcgtggtc aaccagggct ggatcaccct
     1621 gcaggaagcc ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct
     1681 gagagccgcc ctggaactgt tcgagcagga aggcctggcc ccctacctga gcagatggga
     1741 gaagctggac aacttcatca accggcccgt gaaactgatc atcggcgaca aagagatctt
     1801 cggcatcagc cggggcatcg acaagcaggg ggccctgctc ctggaacagg acggcatcat
     1861 caagccctgg atgggcggcg agatcagcct gcggagcgcc gagaagtacc catacgatgt
     1921 tcctgactat gccggtaccg agctcggatc caccatggct agctggagcc acccgcagtt
     1981 cgagaaaggt ggaggttccg gaggtggatc gggaggtgga tcgtggagcc acccgcagtt
     2041 cgaaaaagcg gccgatatca caagtttgta caaaaaagtt ggcatgtctt ccaagcgacc
     2101 agcctctccg tatggggaag cagatggaga ggtagccatg gtgacaagca gacagaaagt
     2161 ggaagaagag gagagtgacg ggctcccagc ctttcacctt cccttgcatg tgagttttcc
     2221 caacaagcct cactctgagg aatttcagcc agtttctctg ctgacgcaag agacttgtgg
     2281 ccataggact cccacttctc agcacaatac aatggaagtt gatggcaata aagttatgtc
     2341 ttcatttgcc ccacacaact catctacctc acctcagaag gcagaagaag gtgggcgaca
     2401 gagtggcgag tccttgtcta gtacagccct gggaactcct gaacggcgca agggcagttt
     2461 agctgatgtt gttgacacct tgaagcagag gaaaatggaa gagctcatca aaaacgagcc
     2521 ggaagaaacc cccagtattg aaaaactact ctcaaaggac tggaaagaca agcttcttgc
     2581 aatgggatcg gggaactttg gcgaaataaa agggactccc gagagcttag ctgagaaaga
     2641 aaggcaactc atgggtatga tcaaccagct gaccagcctc cgagagcagc tgttggctgc
     2701 ccacgatgag cagaagaaac tagctgcctc tcagattgag aaacagcgtc agcaaatgga
     2761 gctggccaag cagcaacaag aacaaattgc aagacagcag cagcagcttc tacagcaaca
     2821 acacaaaatc aatttgctcc agcaacagat ccaggttcaa ggtcagctgc cgccattaat
     2881 gattcccgta ttccctcctg atcaacggac actggctgca gctgcccagc aaggattcct
     2941 cctccctcca ggcttcagct ataaggctgg atgtagtgac ccttaccctg ttcagctgat
     3001 cccaactacc atggcagctg ctgccgcagc aacaccaggc ttaggcccac tccaactgca
     3061 gcagttatat gctgcccagc tagctgcaat gcaggtatct ccaggaggga agctgccagg
     3121 cataccccaa ggcaaccttg gtgctgctgt atctcctacc agcattcaca cagacaagag
     3181 cacaaacagc ccaccaccca aaagcaagga aaaaacaaca ctggagagtc tgactcagca
     3241 actggcagtt aaacagaatg aagaaggaaa atttagccat gcaatgatgg atttcaatct
     3301 gagtggagat tctgatggaa gtgctggagt ctcagagtca agaatttata gggaatcccg
     3361 agggcgtggt agcaatgaac cccacataaa gcgtccaatg aatgccttca tggtgtgggc
     3421 taaagatgaa cggagaaaga tccttcaagc ctttcctgac atgcacaact ccaacatcag
     3481 caagatattg ggatctcgct ggaaagctat gacaaaccta gagaaacagc catattatga
     3541 ggagcaagcc cgtctcagca agcagcacct ggagaagtac cctgactata agtacaagcc
     3601 caggccaaag cgcacctgcc tggtggatgg caaaaagctg cgcattggtg aatacaaggc
     3661 aatcatgcgc aacaggcggc aggaaatgcg gcagtacttc aatgttgggc aacaagcaca
     3721 gatccccatt gccactgctg gtgttgtgta ccctggagcc atcgccatgg ctgggatgcc
     3781 ctcccctcac ctgccctcgg agcactcaag cgtgtctagc agcccagagc ctgggatgcc
     3841 tgttatccag agcacttacg gtgtgaaagg agaggagcca catatcaaag aagagataca
     3901 ggccgaggac atcaatggag aaatttatga tgagtacgac gaggaagagg atgatccaga
     3961 tgtagattat gggagtgaca gtgaaaacca tattgcagac aagccaactg cccaactttc
     4021 ttgtacaaag tggtgacgta agctaggggc ccgtttaaac ccgctgatca gcctcgactg
     4081 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4141 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4201 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4261 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4321 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4561 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa
     4621 gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     4681 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     4741 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     4801 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     4861 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     4921 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     4981 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     5041 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     5101 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     5161 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     5221 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     5281 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     5341 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     5401 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     5461 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     5521 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     5581 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     5641 ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct
     5701 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     5761 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     5821 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5881 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     5941 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6001 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6061 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6121 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6181 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6241 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6301 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6361 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6481 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6541 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6601 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6661 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6721 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6781 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6841 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6901 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6961 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7021 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7081 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7741 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8101 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     8161 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     8221 ggaggtcgct gagtagt
//
