LOCUS       sequence-37046-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S
  TITLE     Tagging muscle cell lineages in development and tail regeneration 
            using Cre recombinase in transgenic Xenopus.
  JOURNAL   Nucleic Acids Res. 2003 Apr 15. 31(8):e44.
  PUBMED    12682379
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Apr 15. 31(8):e44."
COMMENT     Sequence Label: LCMV:ECFP(loxP)EYFP
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..128
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    196..229
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    231..365
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(255..274)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     309..328
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(524..545)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcaggcngg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
       61 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      121 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg
      181 atggatctcc taggtataac ttcgtataat gtatgctata cgaagttatg gatccagaca
      241 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      301 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      361 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      421 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatt
      481 acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
      541 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
      601 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
      661 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt
      721 tcttctgctt gtcggcggtg atatagacgt tgtggctgat gtag
//
