LOCUS       sequence-370462-       10137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding DNMT3A recruiter for methylation reporter
            (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
  TITLE     Base editor scanning charts the DNMT3A activity landscape.
  JOURNAL   Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 
            10.1038/s41589-022-01167-4. Epub 2022 Oct 20.
  PUBMED    36266353
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-022-01167-4"; journalName: "Nat Chem Biol"; date: 
            "2023-02"; volume: "19"; issue: "2"; pages: "176-186"
COMMENT     Sequence Label: pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40
FEATURES             Location/Qualifiers
     source          1..10137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2145..3083
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3040..3060
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3116..3134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3195..3572
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3579..4286
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3724..3742)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3992..4011
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4290..4343
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(6174..6192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6466..6587
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6660..6893
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6965..7086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7126..7261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7282..7300)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7310..7327)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7310..7326)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7319..7341)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7468..7923
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7555..7574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7765..7786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7949..8053
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8054..8122,8123..8914)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8272..8291)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9085..9673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9574..9593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9827..9844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9961..9982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9997..10002,10003..10020,10021..10027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10035..10051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10040..10062
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10059..10075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10059..10075
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10096..10114
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc ctctagagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     1981 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2041 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2101 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2161 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2221 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2281 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2341 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2401 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2461 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2521 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2581 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     2641 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     2701 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     2761 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     2821 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     2881 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     2941 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3001 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3061 aaagtttttt tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta
     3121 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg
     3181 gcggatccgc caccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     3241 agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg
     3301 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     3361 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     3421 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     3481 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     3541 ctaaggccat caccaagtac accagcgcta agcctaggat ggtgagcaag ggcgaggagg
     3601 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3661 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3721 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3781 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatt cccgactact
     3841 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3901 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3961 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     4021 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     4081 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     4141 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     4201 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     4261 ccggcggcat ggacgagctg tacaagtctg agggcagggg gagcctccta acttgcggag
     4321 acgtcgagga gaatcctggc ccatctagac tggacaagag caaagtcata aacggagctc
     4381 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     4441 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     4501 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     4561 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     4621 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     4681 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     4741 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     4801 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     4861 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     4921 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gggtagcggc agcggtagcg
     4981 catgcatggc ggccatccca gccctggacc cagaggccga gcccagcatg gacgtgattt
     5041 tggtgggatc cagtgagctc tcaagctccg tttcacccgg gacaggcaga gatcttattg
     5101 catatgaagt caaggctaac cagcgaaata tagaagacat ctgcatctgc tgcggaagtc
     5161 tccaggttca cacacagcac cctctgtttg agggagggat ctgcgcccca tgtaaggaca
     5221 agttcctgga tgccctcttc ctgtacgacg atgacgggta ccaatcctac tgctccatct
     5281 gctgctccgg agagacgctg ctcatctgcg gaaaccctga ttgcacccga tgctactgct
     5341 tcgagtgtgt ggatagcctg gtcggccccg ggacctcggg gaaggtgcac gccatgagca
     5401 actgggtgtg ctacctgtgc ctgccgtcct cccgaagcgg gctgctgcag cgtcggagga
     5461 agtggcgcag ccagctcaag gccttctacg accgagagtc ggagaatccc cttgagatgt
     5521 tcgaaaccgt gcctgtgtgg aggagacagc cagtccgggt gctgtccctt tttgaagaca
     5581 tcaagaaaga gctgacgagt ttgggctttt tggaaagtgg ttctgacccg ggacaactga
     5641 agcatgtggt tgatgtcaca gacacagtga ggaaggatgt ggaggagtgg ggacccttcg
     5701 atcttgtgta cggcgccaca cctcccctgg gccacacctg tgaccgtcct cccagctggt
     5761 acctgttcca gttccaccgg ctcctgcagt acgcacggcc caagccaggc agccccaggc
     5821 ccttcttctg gatgttcgtg gacaatctgg tgctgaacaa ggaagacctg gacgtcgcat
     5881 ctcgcttcct ggagatggag ccagtcacca tcccagatgt ccacggcgga tccttgcaga
     5941 atgctgtccg cgtgtggagc aacatcccag ccataaggag caggcactgg gctctggttt
     6001 cggaagaaga attgtccctg ctggcccaga acaagcagag ctcgaagctc gcggccaagt
     6061 ggcccaccaa gctggtgaag aactgctttc tccccctaag agaatatttc aagtattttt
     6121 caacagaact cacttcctct ttataaggta ccaggtaagt gtacccaatt cgccctatag
     6181 tgagtcgtat tacaattcac tcgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6241 atggagatcc aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     6301 ttttagattc acagtcccaa ggctcatttc aggcccctca gtcctcacag tctgttcatg
     6361 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     6421 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     6481 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6541 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg tacctttaag
     6601 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     6661 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     6721 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     6781 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     6841 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     6901 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     6961 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7081 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7141 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7201 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7261 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7321 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7381 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7441 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7501 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7561 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7621 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7681 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7741 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7801 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7861 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7921 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7981 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8041 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8101 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8161 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8221 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8281 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8341 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8401 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8461 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8521 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8581 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8641 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8701 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8761 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8821 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8881 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8941 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9001 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9061 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9241 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9301 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9361 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9421 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9481 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9541 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9601 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9661 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9721 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9781 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9841 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9901 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9961 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    10021 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    10081 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagc
//
