LOCUS       sequence-370467-       12008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse transcription factors Klf4 and Gata-3 in an operon 
            from D-mannose promoter for Bacillus subtilis. The secretion peptide
            PhoD is fused to both factors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12008)
  AUTHORS   Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
  TITLE     Engineered endosymbionts that alter mammalian cell surface marker, 
            cytokine and chemokine expression.
  JOURNAL   Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
  PUBMED    36042261
REFERENCE   2  (bases 1 to 12008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-08-30"; volume: "5(1):888. doi"; pages: " 
            10.1038/s42003-022-03851-6"
COMMENT     Sequence Label: pDR111_mannose_Klf4Gata-3
FEATURES             Location/Qualifiers
     source          1..12008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6424..6458)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(6538..6565)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6584..6670)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(10179..10767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10259..10278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10938..11729,11730..11798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11561..11580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11799..11903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11971..11989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatggg atgtcacgca gaattaaata
      661 gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta
      721 tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc taactcatta
      781 ataatccaaa tgagataaaa atgtcatgac attggtttct ttcgtcagct tctggacgaa
      841 ggccggctgc tcgctaagat atgaaagctc gctaaacagc tgctttgtca tagtttgatc
      901 ctcgtgtttg acagccagca taaaaacgag cgaaactttt tcatttcccc attcaagcgg
      961 ctcctttaat gtggcaatcg cgattgctga ttgtttaata aatttagcgt ttgcatgagg
     1021 aatggcaatg cctgacccaa tatttgtagc cgacattttt tccctcatca ccgcatgaac
     1081 cgcatagtcc ttatcaacat agcctttctc aaacaaagct gtcgccagct gttcgattaa
     1141 cttgtagcga tgctccgcct cctgctgcaa aaaaaccaaa aaaggagtcg tattgttcag
     1201 catctgaaac gttttttgtt tttggcggtg tgattcgccc agctgacgta taaacgcact
     1261 taatttcttc tcttcgccag gctctaaaag cggagacaca acaatatggg gaaccgttat
     1321 attctccagc gcaatggtgg atatgacaag atcaatatcc tcatgctttt tgatataatc
     1381 ctttaaatcg gcctttgcaa tacaagccat tacagcgatt tggtgatatt tccgttcaat
     1441 ttttgtccgc agcaattgtg acatcccaat ccccatgtgg cagacgataa ccgccttttt
     1501 atgtgtctcg ctgctgccct gcatccgttc gatcgctgcc tgaaaatgca atgtcaaata
     1561 tgctgcctct tcctcaggaa tatgaagatc aaaagtttga ttgatgtctt ccaaaacatc
     1621 aatgatgagg tggaacaaat aagggtacat ctttttgatg tcattgagca taggatttgc
     1681 tacagaaaga tcgtagctca gccgttgtag cacagtattg agatgaatgt tcaaaccatt
     1741 gatcaaatcc tgatccttgt gaaaatccat cattttcagc tctgaaacac gattgattaa
     1801 atgccctaca accttaggaa gcacagcatt ttcaaggttc tcttctgtct gcaatggata
     1861 acgaacctta ccgcccaata tatgtaatgt taagtacacg gcttcttctt cagggaagcg
     1921 aattgcaaaa accggttcaa gccgctgtaa acaagcaaat gtccattgat attctttctt
     1981 cttttttact gccgccattt cttttgggga caatgaaatc ggctgcttca ttttgatgcg
     2041 gcggaccatt aacaatgtat gcagcaacaa actttcaaac gtttcgtctg tgaaatagag
     2101 ggaatgcttt ttttgtagtg atttgatttc tttcgtcaca aagtctactt cataatgtaa
     2161 aaacttactt ttaataaatt ggctggtaaa cgctgtattg tgaatcaaat cagatattct
     2221 tgccaatgcc tttcttttat ttctttcgtt cccttcaacc tttaatccaa gccgctgcct
     2281 agaaaccagt gtgagatcaa aacgcttcag ccactcttct actgcgtata aatccttttt
     2341 aatggaagat cgattgacaa aatgccgagc ggcaatgtct tttgcggaaa caggcttcgg
     2401 attcattaat aagtcataag caatgtgcag cattctttcc ttatcgctcc gctgcctaga
     2461 agaaaaatgt tcagtatgta attgttggct tagccaggtc ctttcctgtt cttctatatg
     2521 taaataaacg cccaaccctg gttttcgaat aagctgtgca tgagaatgct cattgagata
     2581 atcctcgatc actttcaaat cattccggat tgttttttca gaacactgta ccctatctgc
     2641 gaaatcttgc acaactaaat agtcatcagg ttcagataac aacagataca aaatctcttt
     2701 ttgtcttgta tttatgtatt ccatctgttt ttctatcctt cctgtcagta tctacttgag
     2761 caaattgata gtataagtat actatataag aaaccgttta cattataaaa caaaatcata
     2821 gagacaaaag tttcgttttt taccgaagct tccggaaaaa agcgattttt atactctttg
     2881 agtttgctat gtaatgtata ttcagcagaa attcataaaa atactccaat tcttcaccta
     2941 tttagaacga agattttcta atgtatgagt acgaaagtaa gaacatgatg aagaaaagtg
     3001 atttggtatc ttcggggata aagtgcaggt aacaaaattt ccacacgctc aacaaactaa
     3061 tacccatcaa atgtgtgcta ttaacatttt aaggatggcc attctctctc agcaatcggc
     3121 tagcttagcc catagcagta accatactac ttgggtgatg tgggccaaag ctgagacctg
     3181 aaggtgggtg catgggtgta ggggtagtca gcatatgaga agagtgtgag aaaggagaaa
     3241 tatgagaaag actactcata tggcggctaa gggcggcggg attgaaacta ctgcttttag
     3301 ggaagtcttc caaagcatca tgcaccttct tacacttttt ggatttggaa gacatcttcc
     3361 tattgcgtgt ctgtattcct tcctttttca tggtcagggg tctattaata ttgtgaagct
     3421 tatagtagag cccgcaggca ttacaaactg ggtcaccgtt ggcgtttctg cgccacagtg
     3481 tagtggttgt agtttggcag ttggcgcagg aggtcccggc gcgccgtgct gcggaaagtc
     3541 gtcgcttagg cttgataaga gggcggtttt gcccattcat cttatggtaa aggccgcagg
     3601 cattgcagag ataatgtcca gttccatccc gtctccagag tggtgtactg gtagcgccac
     3661 agttcacaca ttccctacct tcagtgctgg accttgcttt aggtcgggac ttgcatccga
     3721 aacctgttgg tgacccacca agaaggcttg agggagggaa caatccactt gagtactcag
     3781 gaacatatgg tgggtatgtg gtgattgggt gatgagcact gctagaagcg cccccaagtg
     3841 tggtcatact acctctactg tgtgatgtct caagtttcat gctgtcggga agttgaacct
     3901 ggtatttgag acattctttt tcgtcttggc gggcagaacc ggcggaacca ggagtactaa
     3961 gtgagggatc tgggctgacg tctttgggag gggtaggagg aaatgtgaag agatggggac
     4021 tactgtgccc agcagcaagt gatgaacttg atgcaggagg atagacactc agtggtccgg
     4081 gggacccatg gtggatactt gtttttgaaa atggtgaaag attccaaggg gatgcagtgt
     4141 gatgggagct gagagcctta cccccatcga gccaaggcaa agacccgtgc aaaagaggag
     4201 gcctacagac ttggctgcca tggtgggttg gtgggtatct ctgtacagtt gcccgcacag
     4261 aattaccata gtaactaggc acatgattcc cctggccgtc tatattaaac agtacatcaa
     4321 cttcttctgt caggggatac tgtgcctcca tatatgagtg cccaagaccg gggtgatggg
     4381 tatcgggatg ttggccgttc aggactgctg ggtgatggtg gctcacccag cgtggttgat
     4441 cggcagtcac ttccatcgct cccactgatt gcgcaattgt cagtcccagt gacagtcccg
     4501 caatttttcc cgctccttga ataaattttc ttctgtcaaa tgtgttgttt tgaaatgatt
     4561 cttctttcag tttttgcacc cattcgtcaa atcttgagtc atacgccata acttacctcc
     4621 ttcagctttt cgcccaccgg ccgttagaaa tgtcgtttca tatgaagtgc caagtggtcg
     4681 gacctgctaa aggcccggtc acatttctgg cattgaaatg gcctgtgacc agtatgcttt
     4741 ctgtagtgcc ttgtcagttc atcactgcga gcgaatttca tccacatccg tcccagtcac
     4801 agtgataggg cttctcgcct gtgtgtgtgc ggagatgtgc tttaagatga gagcttttag
     4861 tgtaggtctt cccgcaacct gcgtagtcac atgtatgagt ggcagttctc tttcggggcc
     4921 aagagcgtcg accccttttg ggcttgggtt cctctgggag gcaactgcct gggggcataa
     4981 gttcttgata gtgcaaagag gggacctgag attgcatttg gtcaggaagg aatgggggat
     5041 aatttgggcc agggtgaggg tggaatccag ggggaagtgg gagaccggga tggcaatccc
     5101 ggctgttgag caactcttct ggtgacaagg taggggttgt tcgggttggg agctgccgtc
     5161 ccagggggaa atcgtgagtg tttggtcgat gaccattgct aagctgtggc ccagcggaaa
     5221 ggtgagcttc aagactccga ctcacggtac agctgggtac tgcctcttgt tttatcttgg
     5281 ggcacatcct gggagggccc ccggagtaag gagcaactac aacggggtgg cttccgtcag
     5341 gggacccctt ggacacgctt attacgctgg ggctgctata ctcggaacct ggggtggtca
     5401 gactggcttt gagcacgaat ttccccatca agccaccacc agggggttga ggctgctggg
     5461 gaggaatgta tacgggatcc aactcaggtc taaggagctc ggcgacaaat ccgccactgg
     5521 gagatacgtc gttaatatct gccaagttaa atggtgcggt tgggggaggt gcgctctcgc
     5581 gagaatagag cagaccccct ccagtatttg aggctgccac cccagggtcc ccgccggctc
     5641 gaatagggta actgaatgaa caagtggaag gtgcagaggc tgggccagag cttgcgggag
     5701 aagaggaact actagcgctt gcgcttgttg tcactgttgc agcaacactt tcttggtgtg
     5761 tcagtgagtt agacaaaata aaatcaagat caagcaaatc attaaactct tcagtctctc
     5821 tccttgcaag gagagcgggg ttgttacttg agcaggctgc gccagcacct ccagactcaa
     5881 ggtcggtggc gacggtagct gcaagatcgt agggtcgacc tggcaagggt ggcaatcgct
     5941 tcatgtggct aagctcttcc ctccagcggt tggtgggagc gcctgctggc cgaagggtct
     6001 tctcccgtcc tgctggtccg cttgcgaagg tggaaaatga aggcagcagg gcatcggaca
     6061 ctgccatatc tgactcccca ggtggttgtc gcatcgctcc cactgattgc gcaattgtca
     6121 gtcccagtga cagtcccgca atttttcccg ctccttgaat aaattttctt ctgtcaaatg
     6181 tgttgttttg aaatgattct tctttcagtt tttgcaccca ttcgtcaaat cttgagtcat
     6241 acgccataac ttacctcctt ctgtcgactc ctgcaggagg aaacctcctt taaagtgtga
     6301 ataataagat cttgtctcaa ctgtataccg aaatcagctc attaaaatcg ctttttttac
     6361 cataggttcc ggtaataaag gcatttttcc ctataacaaa aaaagcaagg aataatcccc
     6421 tgcataaaaa acgcccggcg gcaaccgagc gttctgaatt aattaatcat cgggaagatc
     6481 ttcatcaccg aaacgcggca ggcagctcta gagttaacaa gagtttgtag aaacgcaaaa
     6541 aggccatccg tcaggatggc cttctgctta gctagagcgg cggatttgtc ctactcagga
     6601 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     6661 gttttatttg atgcctcaag ctagaggtcg aattcctgca gctggcgaat ggcgattttc
     6721 gttcgtgaat acatgttata ataactataa ctaataacgt aacgtgactg gcaagagata
     6781 tttttaaaac aatgaatagg tttacactta ctttagtttt atggaaatga aagatcatat
     6841 catatataat ctagaataaa attaactaaa ataattatta tctagataaa aaatttagaa
     6901 gccaatgaaa tctataaata aactaaatta agtttattta attaacaact atggatataa
     6961 aataggtact aatcaaaata gtgaggagga tatatttgaa tacatacgaa caaattaata
     7021 aagtgaaaaa aatacttcgg aaacatttaa aaaataacct tattggtact tacatgtttg
     7081 gatcaggagt tgagagtgga ctaaaaccaa atagtgatct tgacttttta gtcgtcgtat
     7141 ctgaaccatt gacagatcaa agtaaagaaa tacttataca aaaaattaga cctatttcaa
     7201 aaaaaatagg agataaaagc aacttacgat atattgaatt aacaattatt attcagcaag
     7261 aaatggtacc gtggaatcat cctcccaaac aagaatttat ttatggagaa tggttacaag
     7321 agctttatga acaaggatac attcctcaga aggaattaaa ttcagattta accataatgc
     7381 tttaccaagc aaaacgaaaa aataaaagaa tatacggaaa ttatgactta gaggaattac
     7441 tacctgatat tccattttct gatgtgagaa gagccattat ggattcgtca gaggaattaa
     7501 tagataatta tcaggatgat gaaaccaact ctatattaac tttatgccgt atgattttaa
     7561 ctatggacac gggtaaaatc ataccaaaag atattgcggg aaatgcagtg gctgaatctt
     7621 ctccattaga acatagggag agaattttgt tagcagttcg tagttatctt ggagagaata
     7681 ttgaatggac taatgaaaat gtaaatttaa ctataaacta tttaaataac agattaaaaa
     7741 aattataaaa aaattgaaaa aatggtggaa acactttttt caattttttt gttttattat
     7801 ttaatatttg ggaaatattc attctaattg gtaatcagat tttagaaaac aataaaccct
     7861 tgcatagggg gaatccgttt aggctgggcg gtgatagctt ctcgttcagg cagtacgcct
     7921 cttttctttt ccagacctga gggaggcgga aatggtgtga ggttcccggg gaaaagccaa
     7981 ataggcgatc gcgggagtgc tttatttgaa gatcaggcta tcactgcggt caatagattt
     8041 cacaatgtga tggctggaca gcctgaggaa ctctcgaacc cgaatggaaa caaccagata
     8101 tttatgaatc agcgcggctc acatggcgtt gtgctggcaa atgcaggttc atcctctgtc
     8161 tctatcaata cggcaacaaa attgcctgat ggcaggtatg acaataaagc tggagcgggt
     8221 tcatttcaag tgaacgatgg taaactgaca ggcacgatca atgccaggtc tgtagctgtg
     8281 ctttatcctg atgatattgc aaaagcgcct catgttttcc ttgagaatta caaaacaggt
     8341 gtaacacatt ctttcaatga tcaactgacg attaccttgc gtgcagatgc gaatacaaca
     8401 aaagccgttt atcaaatcaa taatggacca gagacggcgt ttaaggatgg agatcaattc
     8461 acaatcggaa aaggagatcc atttggcaaa acatacacca tcatgttaaa aggaacgaac
     8521 agtgatggtg taacgaggac cgagaaatac agttttgtta aaagagatcc agcgtcggcc
     8581 aaaaccatcg gctatcaaaa tccgaatcat tggagccagg taaatgctta tatctataaa
     8641 catgatggga gccgagtaat tgaattgacc ggatcttggc ctggaaaacc aatgactaaa
     8701 aatgcagacg gaatttacac gctgacgctg cctgcggaca cggatacaac caacgcaaaa
     8761 gtgattttta ataatggcag cgcccaagtg cccggtcaga atcagcctgg ctttgattac
     8821 gtgctaaatg gtttatataa tgactcgggc ttaagcggtt ctcttcccca ttgagggcaa
     8881 ggctagacgg gacttaccga aagaaaccat caatgatggt ttcttttttg ttcataaatc
     8941 agacaaaact tttctcttgc aaaagtttgt gaagtgttgc acaatataaa tgtgaaatac
     9001 ttcacaaaca aaaagacatc aaagagaaac ataccctgca aggatgctga tattgtctgc
     9061 atttgcgccg gagcaaacca aaaacctggt gagacacgcc ttgaattagt agaaaagaac
     9121 ttgaagattt tcaaaggcat cgttagtgaa gtcatggcga gcggatttga cggcattttc
     9181 ttagtcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     9241 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     9301 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
     9361 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     9421 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     9481 tactgaagtg gatttcttta agagctcctt taacttcctc accagtagtt gtatcggtac
     9541 cataagtaga agcagcaacc caagtagctt taccagcatc cggttcaacc agcatagtaa
     9601 gaatcttact ggacatcggc agttcttcga acagtgcgcc aactaccagc tctttctgca
     9661 gttcattcag ggcaccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     9721 tcctcatcct gtctcttgat ccatggatta cgcgttaacc cgggcccgcg gatgcatatg
     9781 atcagatctt aaggcctagg tctagagtct ttgttttgac gccattagcg tacgtaacaa
     9841 tcctcgttaa aggacaagga cctgagcgga agtgtatcgt acagtagacg gagtatacta
     9901 gtatagtcta tagtccgtgg aattattata tttatctccg acgatattct catcagtgaa
     9961 atccagctgg agttctttag caaatttttt tattagctga acttagtatt agtggccata
    10021 ctcctccaat ccaaagctat ttagaaagat tactatatcc tcaaacaggc ggtaaccggc
    10081 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg
    10141 aagtatccag ctcgaggtcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    10621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10861 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10921 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10981 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    11041 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    11101 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    11161 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    11221 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
    11281 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11341 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11401 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11461 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11521 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
    11581 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11641 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11701 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11761 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11821 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11881 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
    11941 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
    12001 caagaatt
//
