LOCUS       sequence-37047-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Ryffel GU, Werdien D, Turan G, Gerhards A, Goosses S, Senkel S
  TITLE     Tagging muscle cell lineages in development and tail regeneration 
            using Cre recombinase in transgenic Xenopus.
  JOURNAL   Nucleic Acids Res. 2003 Apr 15. 31(8):e44.
  PUBMED    12682379
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2003 Apr 15. 31(8):e44."
COMMENT     Sequence Label: LCMV:ECFP(FRT)EYFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..131
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(203..236)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    238..372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(262..281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     316..335
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(531..552)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctaggtgcag gcagggcgct gccggtggtg cagatgaact tcagggtcag cttgccgtag
       61 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      121 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg
      181 gcgatggatc tcctaggccc gggaagttcc tatactttct agagaatagg aacttcggat
      241 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      301 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      361 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      421 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
      481 ctagattact tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      541 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc
      601 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      661 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttggccttg
      721 atgccgttct tctgcttgtc ggcggtgata tagacgttgt ggctgatgta gttgtactcc
      781 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      841 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt ccg
//
