LOCUS       sequence-370470-       11087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses beta-galactosidase via Phyper-spank which is optimized for
            Bacillus subtilis. There is a secretion peptide (PhoD) that is fused
            to beta-galactosidase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
  TITLE     Engineered endosymbionts that alter mammalian cell surface marker, 
            cytokine and chemokine expression.
  JOURNAL   Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
  PUBMED    36042261
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-08-30"; volume: "5(1):888. doi"; pages: " 
            10.1038/s42003-022-03851-6"
COMMENT     Sequence Label: pDR111_Bgal no NLS
FEATURES             Location/Qualifiers
     source          1..11087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(795..1877)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1839..1858
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(2128..5199)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
                     YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
                     DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
                     SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
                     LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
                     WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
                     KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
                     IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
                     WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
                     AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
                     VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
                     FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
                     HMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     5151..5173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5165..5182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5166..5182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5383..5399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(5503..5537)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(5617..5644)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5663..5749)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(9258..9846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9338..9357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10017..10808,10809..10877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10640..10659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10878..10982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11050..11068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatggg atgtcacgca gaattaaata
      661 gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta
      721 tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc taactcacat
      781 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      841 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
      901 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
      961 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     1021 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     1081 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     1141 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     1201 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     1261 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     1321 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     1381 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     1441 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     1501 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgttttacag
     1561 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     1621 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     1681 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     1741 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     1801 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     1861 aacgttactg gtttcatcaa aatcgtctcc ctccgtttga atatttgatt gatcgtaacc
     1921 agatgaagca ctctttccac tatccctaca gtgttatggc ttgaacaatc acgaaacaat
     1981 aattggtacg tacgatcttt cagccgactc aaacatcaaa tcttacaaat gtagtctttg
     2041 aaagtattac atatgtaaga tttaaatgca accgtttttt cggaaggaaa tgatgacctc
     2101 gtttccgccg aattagcttg catgcggtta tttttgacac cagaccaact ggtaatggta
     2161 gcgaccggcg ctcagctgta attccgccga tactgacggg ctccaggagt cgtcgccacc
     2221 aatccccata tggaaaccgt cgatattcag ccatgtgcct tcttccgcgt gcagcagatg
     2281 gcgatggctg gtttccatca gttgctgttg actgtagcgg ctgatgttga actggaagtc
     2341 gccgcgccac tggtgtgggc cataattcaa ttcgcgcgtc ccgcagcgca gaccgttttc
     2401 gctcgggaag acgtacgggg tatacatgtc tgacaatggc agatcccagc ggtcaaaaca
     2461 ggcggcagta aggcggtcgg gatagttttc ttgcggccct aatccgagcc agtttacccg
     2521 ctctgctacc tgcgccagct ggcagttcag gccaatccgc gccggatgcg gtgtatcgct
     2581 cgccacttca acatcaacgg taatcgccat ttgaccacta ccatcaatcc ggtaggtttt
     2641 ccggctgata aataaggttt tcccctgatg ctgccacgcg tgagcggtcg taatcagcac
     2701 cgcatcagca agtgtatctg ccgtgcactg caacaacgct gcttcggcct ggtaatggcc
     2761 cgccgccttc cagcgttcga cccaggcgtt agggtcaatg cgggtcgctt cacttacgcc
     2821 aatgtcgtta tccagcggtg cacgggtgaa ctgatcgcgc agcggcgtca gcagttgttt
     2881 tttatcgcca atccacatct gtgaaagaaa gcctgactgg cggttaaatt gccaacgctt
     2941 attacccagc tcgatgcaaa aatccatttc gctggtggtc agatgcggga tggcgtggga
     3001 cgcggcgggg agcgtcacac tgaggttttc cgccagacgc cactgctgcc aggcgctgat
     3061 gtgcccggct tctgaccatg cggtcgcgtt cggttgcact acgcgtactg tgagccagag
     3121 ttgcccggcg ctctccggct gcggtagttc aggcagttca atcaactgtt taccttgtgg
     3181 agcgacatcc agaggcactt caccgcttgc cagcggctta ccatccagcg ccaccatcca
     3241 gtgcaggagc tcgttatcgc tatgacggaa caggtattcg ctggtcactt cgatggtttg
     3301 cccggataaa cggaactgga aaaactgctg ctggtgtttt gcttccgtca gcgctggatg
     3361 cggcgtgcgg tcggcaaaga ccagaccgtt catacagaac tggcgatcgt tcggcgtatc
     3421 gccaaaatca ccgccgtaag ccgaccacgg gttgccgttt tcatcatatt taatcagcga
     3481 ctgatccacc cagtcccaga cgaagccgcc ctgtaaacgg ggatactgac gaaacgcctg
     3541 ccagtattta gcgaaaccgc caagactgtt acccatcgcg tgggcgtatt cgcaaaggat
     3601 cagcgggcgc gtctctccag gtagcgaaag ccattttttg atggaccatt tcggcacagc
     3661 cgggaagggc tggtcttcat ccacgcgcgc gtacatcggg caaataatat cggtggccgt
     3721 ggtgtcggct ccgccgcctt catactgcac cgggcgggaa ggatcgacag atttgatcca
     3781 gcgatacagc gcgtcgtgat tagcgccgtg gcctgattca ttccccagcg accagatgat
     3841 cacactcggg tgattacgat cgcgctgcac cattcgcgtt acgcgttcgc tcatcgccgg
     3901 tagccagcgc ggatcatcgg tcagacgatt cattggcacc atgccgtggg tttcaatatt
     3961 ggcttcatcc accacataca ggccgtagcg gtcgcacagc gtgtaccaca gcggatggtt
     4021 cggataatgc gaacagcgca cggcgttaaa gttgttctgc ttcatcagca ggatatcctg
     4081 caccatcgtc tgctcatcca tgacctgacc atgcagagga tgatgctcgt gacggttaac
     4141 gcctcgaatc agcaacggct tgccgttcag cagcagcaga ccattttcaa tccgcacctc
     4201 gcggaaaccg acatcgcagg cttctgcttc aatcagcgtg ccgtcggcgg tgtgcagttc
     4261 aaccaccgca cgatagagat tcgggatttc ggcgctccac agtttcgggt tttcgacgtt
     4321 cagacgtagt gtgacgcgat cggcataacc accacgctca tcgataattt caccgccgaa
     4381 aggcgcggtg ccgctggcga cctgcgtttc accctgccat aaagaaactg ttacccgtag
     4441 gtagtcacgc aactcgccgc acatctgaac ttcagcctcc agtacagcgc ggctgaaatc
     4501 atcattaaag cgagtggcaa catggaaatc gctgatttgt gtagtcggtt tatgcagcaa
     4561 cgagacgtca cggaaaatgc cgctcatccg ccacatatcc tgatcttcca gataactgcc
     4621 gtcactccag cgcagcacca tcaccgcgag gcggttttct ccggcgcgta aaaatgcgct
     4681 caggtcaaat tcagacggca aacgactgtc ctggccgtaa ccgacccagc gcccgttgca
     4741 ccacagatga aacgccgagt taacgccatc aaaaataatt cgcgtctggc cttcctgtag
     4801 ccagctttca tcaacattaa atgtgagcga gtaacaaccc gtcggattct ccgtgggaac
     4861 aaacggcgga ttgaccgtaa tgggataggt cacgttggtg tagatgggcg catcgtaacc
     4921 gtgcatctgc cagtttgagg ggacgacgac agtatcggcc tcaggaagat cgcactccag
     4981 ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca aagcgccatt cgccattcag
     5041 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     5101 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     5161 acgttgtaaa acgacggcca gtgaatccgt aatcatggtc gctcccactg attgcgcaat
     5221 tgtcagtccc agtgacagtc ccgcaatttt tcccgctcct tgaataaatt ttcttctgtc
     5281 aaatgtgttg ttttgaaatg attcttcttt cagtttttgc acccattcgt caaatcttga
     5341 gtcatacgcc ataacttacc tccttctgtc gactaagctt aattgttatc cgctcacaat
     5401 tacacacatt atgccacacc ttgtagataa agtcaacaac ttttgcaaaa tgaattgtga
     5461 gtgctcacat ttaccctcga gcaacgttct tgccattgct gcataaaaaa cgcccggcgg
     5521 caaccgagcg ttctgaatta attaatcatc gggaagatct tcatcaccga aacgcggcag
     5581 gcagctctag agttaacaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     5641 ttctgcttag ctagagcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     5701 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctcaagc
     5761 tagaggtcga attcctgcag ctggcgaatg gcgattttcg ttcgtgaata catgttataa
     5821 taactataac taataacgta acgtgactgg caagagatat ttttaaaaca atgaataggt
     5881 ttacacttac tttagtttta tggaaatgaa agatcatatc atatataatc tagaataaaa
     5941 ttaactaaaa taattattat ctagataaaa aatttagaag ccaatgaaat ctataaataa
     6001 actaaattaa gtttatttaa ttaacaacta tggatataaa ataggtacta atcaaaatag
     6061 tgaggaggat atatttgaat acatacgaac aaattaataa agtgaaaaaa atacttcgga
     6121 aacatttaaa aaataacctt attggtactt acatgtttgg atcaggagtt gagagtggac
     6181 taaaaccaaa tagtgatctt gactttttag tcgtcgtatc tgaaccattg acagatcaaa
     6241 gtaaagaaat acttatacaa aaaattagac ctatttcaaa aaaaatagga gataaaagca
     6301 acttacgata tattgaatta acaattatta ttcagcaaga aatggtaccg tggaatcatc
     6361 ctcccaaaca agaatttatt tatggagaat ggttacaaga gctttatgaa caaggataca
     6421 ttcctcagaa ggaattaaat tcagatttaa ccataatgct ttaccaagca aaacgaaaaa
     6481 ataaaagaat atacggaaat tatgacttag aggaattact acctgatatt ccattttctg
     6541 atgtgagaag agccattatg gattcgtcag aggaattaat agataattat caggatgatg
     6601 aaaccaactc tatattaact ttatgccgta tgattttaac tatggacacg ggtaaaatca
     6661 taccaaaaga tattgcggga aatgcagtgg ctgaatcttc tccattagaa catagggaga
     6721 gaattttgtt agcagttcgt agttatcttg gagagaatat tgaatggact aatgaaaatg
     6781 taaatttaac tataaactat ttaaataaca gattaaaaaa attataaaaa aattgaaaaa
     6841 atggtggaaa cacttttttc aatttttttg ttttattatt taatatttgg gaaatattca
     6901 ttctaattgg taatcagatt ttagaaaaca ataaaccctt gcataggggg aatccgttta
     6961 ggctgggcgg tgatagcttc tcgttcaggc agtacgcctc ttttcttttc cagacctgag
     7021 ggaggcggaa atggtgtgag gttcccgggg aaaagccaaa taggcgatcg cgggagtgct
     7081 ttatttgaag atcaggctat cactgcggtc aatagatttc acaatgtgat ggctggacag
     7141 cctgaggaac tctcgaaccc gaatggaaac aaccagatat ttatgaatca gcgcggctca
     7201 catggcgttg tgctggcaaa tgcaggttca tcctctgtct ctatcaatac ggcaacaaaa
     7261 ttgcctgatg gcaggtatga caataaagct ggagcgggtt catttcaagt gaacgatggt
     7321 aaactgacag gcacgatcaa tgccaggtct gtagctgtgc tttatcctga tgatattgca
     7381 aaagcgcctc atgttttcct tgagaattac aaaacaggtg taacacattc tttcaatgat
     7441 caactgacga ttaccttgcg tgcagatgcg aatacaacaa aagccgttta tcaaatcaat
     7501 aatggaccag agacggcgtt taaggatgga gatcaattca caatcggaaa aggagatcca
     7561 tttggcaaaa catacaccat catgttaaaa ggaacgaaca gtgatggtgt aacgaggacc
     7621 gagaaataca gttttgttaa aagagatcca gcgtcggcca aaaccatcgg ctatcaaaat
     7681 ccgaatcatt ggagccaggt aaatgcttat atctataaac atgatgggag ccgagtaatt
     7741 gaattgaccg gatcttggcc tggaaaacca atgactaaaa atgcagacgg aatttacacg
     7801 ctgacgctgc ctgcggacac ggatacaacc aacgcaaaag tgatttttaa taatggcagc
     7861 gcccaagtgc ccggtcagaa tcagcctggc tttgattacg tgctaaatgg tttatataat
     7921 gactcgggct taagcggttc tcttccccat tgagggcaag gctagacggg acttaccgaa
     7981 agaaaccatc aatgatggtt tcttttttgt tcataaatca gacaaaactt ttctcttgca
     8041 aaagtttgtg aagtgttgca caatataaat gtgaaatact tcacaaacaa aaagacatca
     8101 aagagaaaca taccctgcaa ggatgctgat attgtctgca tttgcgccgg agcaaaccaa
     8161 aaacctggtg agacacgcct tgaattagta gaaaagaact tgaagatttt caaaggcatc
     8221 gttagtgaag tcatggcgag cggatttgac ggcattttct tagtcgcgac gcgaggctgg
     8281 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     8341 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     8401 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     8461 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     8521 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct actgaagtgg atttctttaa
     8581 gagctccttt aacttcctca ccagtagttg tatcggtacc ataagtagaa gcagcaaccc
     8641 aagtagcttt accagcatcc ggttcaacca gcatagtaag aatcttactg gacatcggca
     8701 gttcttcgaa cagtgcgcca actaccagct ctttctgcag ttcattcagg gcaccggaga
     8761 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     8821 catggattac gcgttaaccc gggcccgcgg atgcatatga tcagatctta aggcctaggt
     8881 ctagagtctt tgttttgacg ccattagcgt acgtaacaat cctcgttaaa ggacaaggac
     8941 ctgagcggaa gtgtatcgta cagtagacgg agtatactag tatagtctat agtccgtgga
     9001 attattatat ttatctccga cgatattctc atcagtgaaa tccagctgga gttctttagc
     9061 aaattttttt attagctgaa cttagtatta gtggccatac tcctccaatc caaagctatt
     9121 tagaaagatt actatatcct caaacaggcg gtaaccggcc tcttcatcgg gaatgcgcgc
     9181 gaccttcagc atcgccggca tgtccccctg gcggacggga agtatccagc tcgaggtcgg
     9241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9661 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     9721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10321 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10621 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
    11041 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatt
//
