LOCUS       sequence-370474-       12104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses beta-galactosidase by D-mannose promoter for Bacillus 
            subtilis. There is a secretion peptide (PhoD) that is fused to 
            beta-galactosidase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
  TITLE     Engineered endosymbionts that alter mammalian cell surface marker, 
            cytokine and chemokine expression.
  JOURNAL   Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
  PUBMED    36042261
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-08-30"; volume: "5(1):888. doi"; pages: " 
            10.1038/s42003-022-03851-6"
COMMENT     Sequence Label: pDR111_mannose_Bgal no NLS
FEATURES             Location/Qualifiers
     source          1..12104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3120..6191)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
                     YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
                     DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
                     SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
                     LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
                     WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
                     KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
                     IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
                     WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
                     AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
                     VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
                     FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
                     HMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     6143..6165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6157..6174
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6158..6174
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(6520..6554)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(6634..6661)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6680..6766)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(10275..10863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10355..10374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11034..11825,11826..11894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11657..11676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11895..11999)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12067..12085
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatggg atgtcacgca gaattaaata
      661 gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta
      721 tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc taactcatta
      781 ataatccaaa tgagataaaa atgtcatgac attggtttct ttcgtcagct tctggacgaa
      841 ggccggctgc tcgctaagat atgaaagctc gctaaacagc tgctttgtca tagtttgatc
      901 ctcgtgtttg acagccagca taaaaacgag cgaaactttt tcatttcccc attcaagcgg
      961 ctcctttaat gtggcaatcg cgattgctga ttgtttaata aatttagcgt ttgcatgagg
     1021 aatggcaatg cctgacccaa tatttgtagc cgacattttt tccctcatca ccgcatgaac
     1081 cgcatagtcc ttatcaacat agcctttctc aaacaaagct gtcgccagct gttcgattaa
     1141 cttgtagcga tgctccgcct cctgctgcaa aaaaaccaaa aaaggagtcg tattgttcag
     1201 catctgaaac gttttttgtt tttggcggtg tgattcgccc agctgacgta taaacgcact
     1261 taatttcttc tcttcgccag gctctaaaag cggagacaca acaatatggg gaaccgttat
     1321 attctccagc gcaatggtgg atatgacaag atcaatatcc tcatgctttt tgatataatc
     1381 ctttaaatcg gcctttgcaa tacaagccat tacagcgatt tggtgatatt tccgttcaat
     1441 ttttgtccgc agcaattgtg acatcccaat ccccatgtgg cagacgataa ccgccttttt
     1501 atgtgtctcg ctgctgccct gcatccgttc gatcgctgcc tgaaaatgca atgtcaaata
     1561 tgctgcctct tcctcaggaa tatgaagatc aaaagtttga ttgatgtctt ccaaaacatc
     1621 aatgatgagg tggaacaaat aagggtacat ctttttgatg tcattgagca taggatttgc
     1681 tacagaaaga tcgtagctca gccgttgtag cacagtattg agatgaatgt tcaaaccatt
     1741 gatcaaatcc tgatccttgt gaaaatccat cattttcagc tctgaaacac gattgattaa
     1801 atgccctaca accttaggaa gcacagcatt ttcaaggttc tcttctgtct gcaatggata
     1861 acgaacctta ccgcccaata tatgtaatgt taagtacacg gcttcttctt cagggaagcg
     1921 aattgcaaaa accggttcaa gccgctgtaa acaagcaaat gtccattgat attctttctt
     1981 cttttttact gccgccattt cttttgggga caatgaaatc ggctgcttca ttttgatgcg
     2041 gcggaccatt aacaatgtat gcagcaacaa actttcaaac gtttcgtctg tgaaatagag
     2101 ggaatgcttt ttttgtagtg atttgatttc tttcgtcaca aagtctactt cataatgtaa
     2161 aaacttactt ttaataaatt ggctggtaaa cgctgtattg tgaatcaaat cagatattct
     2221 tgccaatgcc tttcttttat ttctttcgtt cccttcaacc tttaatccaa gccgctgcct
     2281 agaaaccagt gtgagatcaa aacgcttcag ccactcttct actgcgtata aatccttttt
     2341 aatggaagat cgattgacaa aatgccgagc ggcaatgtct tttgcggaaa caggcttcgg
     2401 attcattaat aagtcataag caatgtgcag cattctttcc ttatcgctcc gctgcctaga
     2461 agaaaaatgt tcagtatgta attgttggct tagccaggtc ctttcctgtt cttctatatg
     2521 taaataaacg cccaaccctg gttttcgaat aagctgtgca tgagaatgct cattgagata
     2581 atcctcgatc actttcaaat cattccggat tgttttttca gaacactgta ccctatctgc
     2641 gaaatcttgc acaactaaat agtcatcagg ttcagataac aacagataca aaatctcttt
     2701 ttgtcttgta tttatgtatt ccatctgttt ttctatcctt cctgtcagta tctacttgag
     2761 caaattgata gtataagtat actatataag aaaccgttta cattataaaa caaaatcata
     2821 gagacaaaag tttcgttttt taccgaagct tccggaaaaa agcgattttt atactctttg
     2881 agtttgctat gtaatgtata ttcagcagaa attcataaaa atactccaat tcttcaccta
     2941 tttagaacga agattttcta atgtatgagt acgaaagtaa gaacatgatg aagaaaagtg
     3001 atttggtatc ttcggggata aagtgcaggt aacaaaattt ccacacgctc aacaaactaa
     3061 tacccatcaa atgtgtgcta ttaacatttt aaggatggcc attctctctc agcaatcggc
     3121 tatttttgac accagaccaa ctggtaatgg tagcgaccgg cgctcagctg taattccgcc
     3181 gatactgacg ggctccagga gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc
     3241 agccatgtgc cttcttccgc gtgcagcaga tggcgatggc tggtttccat cagttgctgt
     3301 tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg gccataattc
     3361 aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga agacgtacgg ggtatacatg
     3421 tctgacaatg gcagatccca gcggtcaaaa caggcggcag taaggcggtc gggatagttt
     3481 tcttgcggcc ctaatccgag ccagtttacc cgctctgcta cctgcgccag ctggcagttc
     3541 aggccaatcc gcgccggatg cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc
     3601 atttgaccac taccatcaat ccggtaggtt ttccggctga taaataaggt tttcccctga
     3661 tgctgccacg cgtgagcggt cgtaatcagc accgcatcag caagtgtatc tgccgtgcac
     3721 tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc gacccaggcg
     3781 ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg
     3841 aactgatcgc gcagcggcgt cagcagttgt tttttatcgc caatccacat ctgtgaaaga
     3901 aagcctgact ggcggttaaa ttgccaacgc ttattaccca gctcgatgca aaaatccatt
     3961 tcgctggtgg tcagatgcgg gatggcgtgg gacgcggcgg ggagcgtcac actgaggttt
     4021 tccgccagac gccactgctg ccaggcgctg atgtgcccgg cttctgacca tgcggtcgcg
     4081 ttcggttgca ctacgcgtac tgtgagccag agttgcccgg cgctctccgg ctgcggtagt
     4141 tcaggcagtt caatcaactg tttaccttgt ggagcgacat ccagaggcac ttcaccgctt
     4201 gccagcggct taccatccag cgccaccatc cagtgcagga gctcgttatc gctatgacgg
     4261 aacaggtatt cgctggtcac ttcgatggtt tgcccggata aacggaactg gaaaaactgc
     4321 tgctggtgtt ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa gaccagaccg
     4381 ttcatacaga actggcgatc gttcggcgta tcgccaaaat caccgccgta agccgaccac
     4441 gggttgccgt tttcatcata tttaatcagc gactgatcca cccagtccca gacgaagccg
     4501 ccctgtaaac ggggatactg acgaaacgcc tgccagtatt tagcgaaacc gccaagactg
     4561 ttacccatcg cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc aggtagcgaa
     4621 agccattttt tgatggacca tttcggcaca gccgggaagg gctggtcttc atccacgcgc
     4681 gcgtacatcg ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc ttcatactgc
     4741 accgggcggg aaggatcgac agatttgatc cagcgataca gcgcgtcgtg attagcgccg
     4801 tggcctgatt cattccccag cgaccagatg atcacactcg ggtgattacg atcgcgctgc
     4861 accattcgcg ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc ggtcagacga
     4921 ttcattggca ccatgccgtg ggtttcaata ttggcttcat ccaccacata caggccgtag
     4981 cggtcgcaca gcgtgtacca cagcggatgg ttcggataat gcgaacagcg cacggcgtta
     5041 aagttgttct gcttcatcag caggatatcc tgcaccatcg tctgctcatc catgacctga
     5101 ccatgcagag gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc
     5161 agcagcagca gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct
     5221 tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt
     5281 tcggcgctcc acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggcataa
     5341 ccaccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt
     5401 tcaccctgcc ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga
     5461 acttcagcct ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa
     5521 tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc
     5581 cgccacatat cctgatcttc cagataactg ccgtcactcc agcgcagcac catcaccgcg
     5641 aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg
     5701 tcctggccgt aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca
     5761 tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc
     5821 gagtaacaac ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag
     5881 gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg
     5941 acagtatcgg cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc
     6001 ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatcc
     6181 gtaatcatgg tcgctcccac tgattgcgca attgtcagtc ccagtgacag tcccgcaatt
     6241 tttcccgctc cttgaataaa ttttcttctg tcaaatgtgt tgttttgaaa tgattcttct
     6301 ttcagttttt gcacccattc gtcaaatctt gagtcatacg ccataactta cctccttctg
     6361 tcgactaagc ttaaggaaac ctcctttaaa gtgtgaataa taagatcttg tctcaactgt
     6421 ataccgaaat cagctcatta aaatcgcttt ttttaccata ggttccggta ataaaggcat
     6481 ttttccctat aacaaaaaaa gcaaggaata atcccctgca taaaaaacgc ccggcggcaa
     6541 ccgagcgttc tgaattaatt aatcatcggg aagatcttca tcaccgaaac gcggcaggca
     6601 gctctagagt taacaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
     6661 tgcttagcta gagcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     6721 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctcaagctag
     6781 aggtcgaatt cctgcagctg gcgaatggcg attttcgttc gtgaatacat gttataataa
     6841 ctataactaa taacgtaacg tgactggcaa gagatatttt taaaacaatg aataggttta
     6901 cacttacttt agttttatgg aaatgaaaga tcatatcata tataatctag aataaaatta
     6961 actaaaataa ttattatcta gataaaaaat ttagaagcca atgaaatcta taaataaact
     7021 aaattaagtt tatttaatta acaactatgg atataaaata ggtactaatc aaaatagtga
     7081 ggaggatata tttgaataca tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac
     7141 atttaaaaaa taaccttatt ggtacttaca tgtttggatc aggagttgag agtggactaa
     7201 aaccaaatag tgatcttgac tttttagtcg tcgtatctga accattgaca gatcaaagta
     7261 aagaaatact tatacaaaaa attagaccta tttcaaaaaa aataggagat aaaagcaact
     7321 tacgatatat tgaattaaca attattattc agcaagaaat ggtaccgtgg aatcatcctc
     7381 ccaaacaaga atttatttat ggagaatggt tacaagagct ttatgaacaa ggatacattc
     7441 ctcagaagga attaaattca gatttaacca taatgcttta ccaagcaaaa cgaaaaaata
     7501 aaagaatata cggaaattat gacttagagg aattactacc tgatattcca ttttctgatg
     7561 tgagaagagc cattatggat tcgtcagagg aattaataga taattatcag gatgatgaaa
     7621 ccaactctat attaacttta tgccgtatga ttttaactat ggacacgggt aaaatcatac
     7681 caaaagatat tgcgggaaat gcagtggctg aatcttctcc attagaacat agggagagaa
     7741 ttttgttagc agttcgtagt tatcttggag agaatattga atggactaat gaaaatgtaa
     7801 atttaactat aaactattta aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg
     7861 gtggaaacac ttttttcaat ttttttgttt tattatttaa tatttgggaa atattcattc
     7921 taattggtaa tcagatttta gaaaacaata aacccttgca tagggggaat ccgtttaggc
     7981 tgggcggtga tagcttctcg ttcaggcagt acgcctcttt tcttttccag acctgaggga
     8041 ggcggaaatg gtgtgaggtt cccggggaaa agccaaatag gcgatcgcgg gagtgcttta
     8101 tttgaagatc aggctatcac tgcggtcaat agatttcaca atgtgatggc tggacagcct
     8161 gaggaactct cgaacccgaa tggaaacaac cagatattta tgaatcagcg cggctcacat
     8221 ggcgttgtgc tggcaaatgc aggttcatcc tctgtctcta tcaatacggc aacaaaattg
     8281 cctgatggca ggtatgacaa taaagctgga gcgggttcat ttcaagtgaa cgatggtaaa
     8341 ctgacaggca cgatcaatgc caggtctgta gctgtgcttt atcctgatga tattgcaaaa
     8401 gcgcctcatg ttttccttga gaattacaaa acaggtgtaa cacattcttt caatgatcaa
     8461 ctgacgatta ccttgcgtgc agatgcgaat acaacaaaag ccgtttatca aatcaataat
     8521 ggaccagaga cggcgtttaa ggatggagat caattcacaa tcggaaaagg agatccattt
     8581 ggcaaaacat acaccatcat gttaaaagga acgaacagtg atggtgtaac gaggaccgag
     8641 aaatacagtt ttgttaaaag agatccagcg tcggccaaaa ccatcggcta tcaaaatccg
     8701 aatcattgga gccaggtaaa tgcttatatc tataaacatg atgggagccg agtaattgaa
     8761 ttgaccggat cttggcctgg aaaaccaatg actaaaaatg cagacggaat ttacacgctg
     8821 acgctgcctg cggacacgga tacaaccaac gcaaaagtga tttttaataa tggcagcgcc
     8881 caagtgcccg gtcagaatca gcctggcttt gattacgtgc taaatggttt atataatgac
     8941 tcgggcttaa gcggttctct tccccattga gggcaaggct agacgggact taccgaaaga
     9001 aaccatcaat gatggtttct tttttgttca taaatcagac aaaacttttc tcttgcaaaa
     9061 gtttgtgaag tgttgcacaa tataaatgtg aaatacttca caaacaaaaa gacatcaaag
     9121 agaaacatac cctgcaagga tgctgatatt gtctgcattt gcgccggagc aaaccaaaaa
     9181 cctggtgaga cacgccttga attagtagaa aagaacttga agattttcaa aggcatcgtt
     9241 agtgaagtca tggcgagcgg atttgacggc attttcttag tcgcgacgcg aggctggatg
     9301 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     9361 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     9421 cttaccagcc taacttcgat cattggaccg ctgatcgtca cggcgattta tgccgcctcg
     9481 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     9541 cccgcgttgc gtcgcggtgc atggagccgg gccacctact gaagtggatt tctttaagag
     9601 ctcctttaac ttcctcacca gtagttgtat cggtaccata agtagaagca gcaacccaag
     9661 tagctttacc agcatccggt tcaaccagca tagtaagaat cttactggac atcggcagtt
     9721 cttcgaacag tgcgccaact accagctctt tctgcagttc attcagggca ccggagaacc
     9781 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatccat
     9841 ggattacgcg ttaacccggg cccgcggatg catatgatca gatcttaagg cctaggtcta
     9901 gagtctttgt tttgacgcca ttagcgtacg taacaatcct cgttaaagga caaggacctg
     9961 agcggaagtg tatcgtacag tagacggagt atactagtat agtctatagt ccgtggaatt
    10021 attatattta tctccgacga tattctcatc agtgaaatcc agctggagtt ctttagcaaa
    10081 tttttttatt agctgaactt agtattagtg gccatactcc tccaatccaa agctatttag
    10141 aaagattact atatcctcaa acaggcggta accggcctct tcatcgggaa tgcgcgcgac
    10201 cttcagcatc gccggcatgt ccccctggcg gacgggaagt atccagctcg aggtcgggcc
    10261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    11041 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    11101 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    11161 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    11221 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    11281 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    11341 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11401 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11461 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11521 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11581 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11641 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11701 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11761 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11821 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11881 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11941 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    12001 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    12061 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aatt
//
