LOCUS       sequence-370475-        8970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral destination vector for cloning desired protein as rTetR 
            fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR,
            with SV40) for expression in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
  TITLE     Base editor scanning charts the DNMT3A activity landscape.
  JOURNAL   Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 
            10.1038/s41589-022-01167-4. Epub 2022 Oct 20.
  PUBMED    36266353
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-022-01167-4"; journalName: "Nat Chem Biol"; date: 
            "2023-02"; volume: "19"; issue: "2"; pages: "176-186"
COMMENT     Sequence Label: pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
FEATURES             Location/Qualifiers
     source          1..8970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1806..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2145..3083
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3040..3060
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3116..3134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3195..3572
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3579..4286
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3724..3742)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3992..4011
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4290..4343
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        complement(5013..5031)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5305..5426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5493..5726
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5798..5919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5959..6094
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6115..6133)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6143..6160)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6143..6159)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6152..6174)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6301..6756
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6388..6407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6598..6619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6782..6886
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6887..6955,6956..7747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7105..7124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7918..8506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8407..8426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8660..8677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8794..8815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8830..8835,8836..8853,8854..8860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8868..8884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8873..8895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8892..8908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8892..8908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8929..8947
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     1741 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg
     1801 ttaactttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1861 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1921 tttatcgatc ctctagagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     1981 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2041 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2101 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2161 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2221 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2281 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2341 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2401 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2461 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2521 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2581 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     2641 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     2701 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     2761 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     2821 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     2881 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     2941 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3001 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3061 aaagtttttt tcttccattt caggtgtcgt gagctagcga ttacgccaag ctcgaaatta
     3121 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagcccgg
     3181 gcggatccgc caccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     3241 agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg
     3301 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     3361 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     3421 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     3481 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     3541 ctaaggccat caccaagtac accagcgcta agcctaggat ggtgagcaag ggcgaggagg
     3601 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3661 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3721 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3781 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatt cccgactact
     3841 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3901 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3961 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     4021 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     4081 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     4141 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     4201 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     4261 ccggcggcat ggacgagctg tacaagtctg agggcagggg gagcctccta acttgcggag
     4321 acgtcgagga gaatcctggc ccatctagac tggacaagag caaagtcata aacggagctc
     4381 tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc
     4441 tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg
     4501 ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt
     4561 catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc
     4621 gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg
     4681 aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc
     4741 tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag
     4801 caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa
     4861 ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca
     4921 tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gggtagcggc agcggtagcg
     4981 catgcggtac caggtaagtg tacccaattc gccctatagt gagtcgtatt acaattcact
     5041 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggagatcca atttttaagt
     5101 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattca cagtcccaag
     5161 gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag ccataccaca
     5221 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5281 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5341 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5401 ttgtccaaac tcatcaatgt atcttaattt aagaccaatg acttacaagg cagctgtaga
     5461 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5521 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5581 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5641 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5701 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5761 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5821 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5881 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5941 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6001 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     6061 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata
     6121 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     6181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     6241 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg
     6301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6721 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6781 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6841 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6901 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6961 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7021 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7081 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7141 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7201 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7261 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7321 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7381 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7441 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7501 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7561 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7621 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7681 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7741 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7801 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7861 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7921 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7981 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8041 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8101 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8161 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8221 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8281 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8341 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8401 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8461 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8521 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8581 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8641 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8701 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8761 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8821 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8881 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     8941 ctaaagggaa caaaagctgg agctgcaagc
//
