LOCUS       sequence-370478-        9995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GPI-anchored form of mouse Nrx3-alpha containing the 
            alternative splice site 5 together with EGFP protein separated by 2A
            peptide. Contains insertions at alternatively splice sites 1,3,.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9995)
  AUTHORS   Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, 
            Bischofberger J, Scheiffele P
  TITLE     Targeted proteoform mapping uncovers specific Neurexin-3 variants 
            required for dendritic inhibition.
  JOURNAL   Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017.
  PUBMED    35550065
REFERENCE   2  (bases 1 to 9995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 May 8. pii: S0896-6273(22)00362-2. doi: 
            10.1016/j.neuron.2022.04.017."
COMMENT     Sequence Label: pDisplay-EGFP-2A-HA-Nrxn3-alpha-
            2(-)/3(+)/4(+...
FEATURES             Location/Qualifiers
     source          1..9995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     638..657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(688..690,691..693,694..1404)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(733..754)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(994..1013)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1341..1362
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1429..1482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     sig_peptide     1507..1566
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             1570..1596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(5746..5763)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5752..5976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6108..6696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6188..6207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7025..7072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     7097..7116
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(7304..8098)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(7304..8098)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7415..7434)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8025..8044
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     enhancer        8295..8486
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8443..8463
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(8546..9337,9338..9406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9169..9188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9407..9511)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9538..9993
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9625..9644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9835..9856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagcttg gtaccgagct cggatctatg gtgagcaagg gcgaggagct gttcaccggg
      721 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      781 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      841 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      901 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      961 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1021 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1081 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1141 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1201 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1261 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1321 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1381 ctcggcatgg acgagctgta caagtccgga ctcagatccg gcagtggaga gggcagagga
     1441 agtctgctaa catgcggtga cgtcgaggag aatcctggcc cagaattcgg cttggggata
     1501 tccaccatgg agacagacac actcctgcta tgggtactgc tgctctgggt tccaggttcc
     1561 actggtgact atccatatga tgttccagat tatgctgggg cccagccggc cagatctttc
     1621 atgggccttc ctaaccagtg ggcccgctac ctgcgttggg atgcaagcac gcgcagtgac
     1681 ctgagcttcc agttcaagac caatgtttcc actgggctgc tcctgtattt ggatgatggt
     1741 ggtgtctgtg acttcctctg tctctccctg gtggatggcc gcgttcagct ccgctttagc
     1801 atggactgtg ccgagaccac tgtgctatcc aacaagcagg tgaacgacag cagttggcac
     1861 ttcctcatgg tgagccgtga tcgtgtgcgc actgggctgg tgattgatgg tgagggccag
     1921 tctggagagc tacggcccca gcggccttat atggatgtgg tcagtgattt gttcctcggt
     1981 ggcgtccccg ctgacattcg accttctgcc ctgaccctcg atggagtaca gagcatgcct
     2041 ggctttaagg gattaatgct ggatctcaag tatggcaact cggaacctcg gcttctgggg
     2101 agccagagcg tccagttaga agcagaggga ccctgtggcg agcgtccctg tgaaaatggt
     2161 gggatctgct tcctcttgga tggtcatccc acctgtgact gttctaccac tggctatggt
     2221 ggcacactct gctcagaaga tgtcagtcaa ggtccaggcc tctcccatct tatgatgagt
     2281 gaacaagcac gagaggagaa cgtggccacc ttccgaggct cagagtatct gtgctatgac
     2341 ctgtcccaga accccatcca gagcagcagc gacgaaatca ccctttcctt taagacctgg
     2401 caacgcaatg ggctcatcct ccacactggc aagtcggccg actatgttaa cctggctctg
     2461 aaggatggtg cggtctcctt ggtcattaac ctggggtccg gggcctttga ggccattgtg
     2521 gagccagtga atgggaaatt caacgacaac gcctggcatg atgtcaaagt gacacgcaat
     2581 cttcggcagg tgacaatctc tgtggatggc atcctaacca caacgggcta cactcaagag
     2641 gactacacca tgctgggctc agatgacttc ttctatgttg gtggaagccc aagtaccgca
     2701 gacttgccag gttcacctgt gagcaacaac ttcatgggct gccttaaaga ggttgtttat
     2761 aagaataatg acatccgtct agagctgtct cgcttggccc ggattggaga caccaagatg
     2821 aaaatctatg gtgaagttgt gttcaagtgt gagaacgtgg ccacactgga tcccatcaac
     2881 tttgagaccc cagaggctta catcagcttg cccaagtgga acaccaaacg tatgggctcc
     2941 atttcctttg actttcgcac cactgagccc aacggcctga ttctcttcac tcacgggaag
     3001 cctcaagaaa ggaaagatgt ccggagccaa aagaacacaa aagttgactt ctttgctgtg
     3061 gaacttcttg atggcaacct gtatttgctg cttgacatgg gctctggcac catcaaagtg
     3121 aaggccactc agaagaaggc caatgatggg gaatggtacc acgtggatat tcagcgtgat
     3181 ggcagatcag gtaccatatc tgtgaacagc aggcgcacgc cattcaccgc cagtggggag
     3241 agtgaaattc tcgacctgga gggggacatg tatctgggcg ggctgccaga gaaccgagct
     3301 ggcctcatcc tccccacgga gctctggacc gccatgctca actacggtta cgtgggctgc
     3361 atccgcgacc tgttcatcga tgggcgcagc aagaacatcc ggcagctggc ggagatgcag
     3421 aacgcagcag gcgtcaagtc ctcctgttca cgcatgagcg ccaagcagtg tgacagctac
     3481 ccctgcaaga acaacgcggt gtgcaaggac ggctggaacc gcttcatctg cgactgcact
     3541 ggcaccggct actgggggag aacctgcgaa agagaggcat ctatcctgag ctatgacggc
     3601 agcatgtaca tgaaggtcat catgcccatg gtgatgcaca cggaggcaga ggatgtgtcc
     3661 ttccgcttca tgtcacagcg agcctatggg ctactggtag ccacaacctc cagggattct
     3721 gcagatacac tgcgtctaga gttggatggt gggcgtgtca agctcatggt taacttagac
     3781 tgtatcagga taaactgtaa ctccagcaaa ggacctgaga ccctgtatgc agggcagaag
     3841 ctcaatgaca atgagtggca caccgtccgg gtagtgcgga gaggaaaaag ccttaagcta
     3901 acagtggatg atgatgtggc tgaggggaca atggtgggcg accacacccg cctggaattc
     3961 cacaatattg aaactggtat catgactgag aagcgttaca tctctgtggt cccctccagt
     4021 ttcattggcc atctacagag cctcatgttc aatggcttac tgtacattga tttgtgcaaa
     4081 aatggcgaca tcgactactg tgaactgaag gctcgctttg gactgagaaa catcatcgcc
     4141 gaccctgtca cttttaagac caagagcagc tacctgaccc tcgccaccct acaggcttac
     4201 acctccatgc acctcttctt ccagttcaag accacttcag ctgatggctt cattctcttc
     4261 aatagcggag atggcaatga ttttattgca gttgagctgg tcaaggggta catacactat
     4321 gtgtttgatc tcggcaatgg tcccaatgtg atcaaaggca acagtgaccg ccccctgaat
     4381 gacaaccagt ggcacaacgt ggtcatcacc agggacagca gtaacaccca cagtctgaag
     4441 gtggacacca aggtagtcac tcaggtcatc aatggtgcca aaaatctgga tttgaaaggt
     4501 gacctctata tggctggctt agcccagggc atgtacagca accttcccaa gcttgtggcc
     4561 tccagggatg gatttcaggg ctgcctggcc tctgtggact tgaatggacg cctgcctgat
     4621 ctcatcaacg atgctctcca caggagtgga cagatcgagc gaggctgtga aggacccagt
     4681 acaacctgtc aggaggattc atgcgccaac cagggggttt gcatgcagca gtgggaaggt
     4741 ttcacctgtg actgctccat gacatcatat tctggaaacc agtgcaatga ccctggtgca
     4801 acatacatct ttgggaaaag cggtggcctc atcctctata cctggccagc aaatgacaga
     4861 cccagcacac gctctgaccg tctcgccgtg ggcttcagca ctactgtgaa ggatggtatc
     4921 ttagtacgca ttgacagcgc ccctggactt ggcgacttcc tccagcttca catagaacaa
     4981 gggaaaattg gagttgtctt caatattggc acagttgaca tctccatcaa agaagagaga
     5041 actcctgtca atgatggcaa ataccacgtt gtgcgcttca ccaggaatgg gggaaatgct
     5101 acacttcagg tggacaactg gccagtgaat gagcactatc ctacaggcaa cactgataat
     5161 gaacgcctcc aaatggtaaa acagaaaatc cccttcaaat ataaccggcc cgtagaggag
     5221 tggctgcagg aaaaaggccg gcagctaacc atcttcaaca cccaggcgca aatagccatc
     5281 ggaggaaagg acaaaggacg tctctttcaa ggtcaactct ctgggctcta ttatgatggt
     5341 ttgaaagtac tgaacatggc agctgagaac aaccctaata ttaaaatcaa tggaagtgtc
     5401 cggctagtgg gagaagtccc atcagtctca ggaacaacac agacaacgtc catgccacct
     5461 gaaatgtcta ccaccgtcat ggaaaccacc accaccatgg ctacgaccac cacccgaaag
     5521 aaccgctcta cagccagcat tcagcccacg tcagatgatc ttgtttcatc tgctgaatgt
     5581 tcaagtgatg atgaagactt tgtcgaatgt gaaccaagta cagccagaag ctctaatgca
     5641 gctagatcac tccgtgccgc ccttacatgg acatggcgac tcacttacac acttacccct
     5701 ctcatcttca tctcctgtgt agtacactca tagctcgagg atcagcctcg actgtgcctt
     5761 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5821 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5881 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5941 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagtg
     6001 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6061 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6541 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6841 atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc tgcgagccct
     6901 gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg ggcgtattgg
     6961 ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg aaccccgcgt
     7021 ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     7081 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct agggtgggcg
     7141 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     7201 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tgatggcagg
     7261 ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag aactcgtcaa
     7321 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     7381 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     7441 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     7501 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     7561 cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     7621 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     7681 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     7741 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     7801 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     7861 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca
     7921 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     7981 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     8041 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     8101 gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag
     8161 cctcctcact acttctggaa tagctcagag gccgggcctc ggcctctgca taaataaaaa
     8221 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     8281 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     8341 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     8401 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     8461 tccacagctg gttctttccg cctcaggact cttccttttt caataaatca atctaaagta
     8521 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8581 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8641 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8701 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8761 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8821 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8881 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8941 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9001 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9061 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9121 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9181 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9241 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9301 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9361 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9421 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9481 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     9601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     9661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     9721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     9781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     9841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     9901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     9961 cgaattttaa caaaatatta acgcttacaa tttac
//
