LOCUS       sequence-370479-        7869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human PAX7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Goos H, Kinnunen M, Salokas K, Tan Z, Liu X, Yadav L, Zhang Q, Wei 
            GH, Varjosalo M
  TITLE     Human transcription factor protein interaction networks.
  JOURNAL   Nat Commun. 2022 Feb 9;13(1):766. doi: 10.1038/s41467-022-28341-5.
  PUBMED    35140242
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-02-9"; volume: "13(1):766. doi"; pages: " 
            10.1038/s41467-022-28341-5"
COMMENT     Sequence Label: MAC_N_PAX7
FEATURES             Location/Qualifiers
     source          1..7869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(130..718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(210..229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(889..1680,1681..1749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1512..1531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1750..1854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1929..1948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2120..2499
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2500..2703
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2654..2674
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2705..2723
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2726..2744
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2754..2778
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2946..3296,3297..3299,3300..3908)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3909..3935
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3966..3989
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4026..4049
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    4062..4086
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4087..4096
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5644..5668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5706..5723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5712..5936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6069..6088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6220..6267
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6275..7297
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6275..7297
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7427..7548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7464..7483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7518..7537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7597..7613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7597..7613)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7610..7632)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7621..7637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7645..7651,7652..7669,7670..7675))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7690..7711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7828..7845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
       61 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
      661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
      721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
      781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     1021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     1081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     1141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     1201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     1261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     1321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     1381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     1441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     1501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     1561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     1621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     1681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1861 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     1921 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     1981 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     2041 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     2101 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2161 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2221 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2281 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2341 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2401 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2461 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2521 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2581 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2641 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2701 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     2761 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2821 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg taccgagctc
     2881 ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg
     2941 agaccatgaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt
     3001 tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc atcaacaagc
     3061 acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc aagggctaca
     3121 gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag ctggatggcg
     3181 gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc gaccggatcg
     3241 gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc agaggcggca
     3301 ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg ttctggagac
     3361 tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc gtgatggccg
     3421 aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac gacctgtatc
     3481 tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca ggcgacgccg
     3541 cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa gagagcgtgg
     3601 tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg aacaccctgg
     3661 ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag gaaggcctgg
     3721 ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc gtgaaactga
     3781 tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag ggggccctgc
     3841 tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc ctgcggagcg
     3901 ccgagaagta cccatacgat gttcctgact atgccggtac cgagctcgga tccaccatgg
     3961 ctagctggag ccacccgcag ttcgagaaag gtggaggttc cggaggtgga tcgggaggtg
     4021 gatcgtggag ccacccgcag ttcgaaaaag cggccgatat cacaagtttg tacaaaaaag
     4081 ttggcaccat ggcggccctt cccggcacgg taccgagaat gatgcggccg gctccggggc
     4141 agaactaccc ccgcacggga ttccctttgg aagtgtccac cccgcttggc caaggccggg
     4201 tcaatcagct gggaggggtc ttcatcaatg ggcgacccct gcctaaccac atccgccaca
     4261 agatagtgga gatggcccac catggcatcc ggccctgtgt catctcccga cagctgcgtg
     4321 tctcccacgg ctgcgtctcc aagattcttt gccgctacca ggagaccggg tccatccggc
     4381 ctggggccat cggcggcagc aagcccagac aggtggcgac tccggatgta gagaaaaaga
     4441 ttgaggagta caagagggaa aacccaggca tgttcagctg ggagatccgg gacaggctgc
     4501 tgaaggatgg gcactgtgac cgaagcactg tgccctcagt gagttcgatt agccgcgtgc
     4561 tcagaatcaa gttcgggaag aaagaggagg aggatgaagc ggacaagaag gaggacgacg
     4621 gcgaaaagaa ggccaaacac agcatcgacg gcatcctggg cgacaaaggg aaccggctgg
     4681 acgagggctc ggatgtggag tcggaacctg acctcccact gaagcgcaag cagcgacgca
     4741 gtcggaccac attcacggcc gagcagctgg aggagctgga gaaggccttt gagaggaccc
     4801 actacccaga catatacacc cgcgaggagc tggcgcagag gaccaagctg acagaggcgc
     4861 gtgtgcaggt ctggttcagt aaccgccgcg cccgttggcg taagcaggca ggagccaacc
     4921 agctggcggc gttcaaccac cttctgccag gaggcttccc gcccaccggc atgcccacgc
     4981 tgccccccta ccagctgccg gactccacct accccaccac caccatctcc caagatgggg
     5041 gcagcactgt gcaccggcct cagcccctgc caccgtccac catgcaccag ggcgggctgg
     5101 ctgcagcggc tgcagccgcc gacaccagct ctgcctacgg agcccgccac agcttctcca
     5161 gctactctga cagcttcatg aatccggcgg cgccctccaa ccacatgaac ccggtcagca
     5221 acggcctgtc tcctcaggtg atgagcatct tgggcaaccc cagtgcggtg cccccgcagc
     5281 cacaggctga cttctccatc tccccgctgc atggcggcct ggactcggcc acctccatct
     5341 cagccagctg cagccagcgg gccgactcca tcaagccagg agacagcctg cccacctccc
     5401 aggcctactg cccacccacc tacagcacca ccggctacag cgtggacccc gtggccggct
     5461 atcagtacgg ccagtacggc cagagtgagt gcctggtgcc ctgggcgtcc cccgtcccca
     5521 ttccttctcc cacccccagg gcctcctgct tgtttatgga gagctacaag gtggtgtcag
     5581 ggtggggaat gtccatttca cagatggaaa aattgaagtc cagccagatg gaacagttca
     5641 ccttgccaac tttcttgtac aaagtggtga cgtaagctag gggcccgttt aaacccgctg
     5701 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5761 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5821 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5881 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5941 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6001 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6061 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6121 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6181 ccccaaaaaa cttgattagg gtgatggttc acgtacctag aagttcctat tccgaagttc
     6241 ctattctcta gaaagtatag gaacttcctt ggccaaaaag cctgaactca ccgcgacgtc
     6301 tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga
     6361 gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt
     6421 aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc
     6481 cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg
     6541 catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc
     6601 tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac
     6661 gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt
     6721 catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt
     6781 cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga
     6841 agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg
     6901 cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc
     6961 caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga
     7021 gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg
     7081 tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca
     7141 gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc
     7201 ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa
     7261 ccgacgcccc agcactcgtc cgagggcaaa ggaatagcac gtactacgag atttcgattc
     7321 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7381 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7561 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7861 gctgcggcg
//
