LOCUS       sequence-370480-       10863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human LMTK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_LMTK2
FEATURES             Location/Qualifiers
     source          1..10863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        144..523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        524..727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     678..698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    729..747
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    750..768
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     778..802
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    945..969
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5509..5533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5580..5603
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5640..5663
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5694..5720
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5694..5720
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5721..6068,6069..6071,6072..6680)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6724..6741)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6730..6954
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7238..7285
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7293..8315
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7293..8315
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8445..8566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8482..8501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8536..8555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8615..8631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8615..8631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8628..8650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8639..8655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8663..8669,8670..8687,8688..8693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8708..8729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8846..8863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9017..9605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9097..9116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9776..10567,10568..10636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10399..10418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10637..10741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10816..10835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
       61 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      121 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      181 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      241 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      301 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      361 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      421 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      481 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      541 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      601 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      661 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      721 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
      781 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
      841 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga
      901 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
      961 aagcagaagg gccgtcaagg cccaccatgc cggggccgcc ggcgttgcgg cggaggctgc
     1021 tgctgctgct gctggtcctc ctgatcgccg gcagtgctgg ggccgcgcca cttccgcaaa
     1081 caggtgcagg ggaggcgcca cctgctgcag aagtttcctc atcttttgtg atcctgtgtg
     1141 tgtgcagttt aataatatta atagtgttaa ttgcaaactg tgtatcctgc tgtaaggacc
     1201 cagaaataga ctttaaggaa tttgaagata attttgatga tgagatagat ttcacaccac
     1261 cagcagaaga cactccctct gttcagtccc cagcagaggt cttcacactt tcagtaccaa
     1321 atatttcact cccagctccc tcgcaattcc agccttctgt agagggattg aagtctcaag
     1381 ttgcccgcca cagtctaaac tacatacagg aaattggaaa tggctggttt ggaaaggttc
     1441 tcttgggaga gatttacacg ggcactagcg tagcaagagt catcgtgaag gagttaaaag
     1501 caagtgccaa cccaaaggaa caagatactt ttttgaaaaa tggagaacct tactacattc
     1561 ttcagcatcc aaatattctt cagtgtgttg gacagtgcgt agaagcgatt ccctacctcc
     1621 tggtgtttga gttctgtgac ttgggtgacc tgaaggcgta tctgcgcagc gagcaggagc
     1681 acatgcgggg ggactcacag accatgctgc tgcagaggat ggcgtgcgag gtcgccgcgg
     1741 ggctggccgc catgcacaag ctgcacttcc tgcacagtga tttagccctg cggaattgtt
     1801 ttctcacctc cgacttaaat gtgaaagtgg gagattacgg aataggattc agcaggtaca
     1861 aggaggatta tattgaaaca gatgataaaa aagttttccc tctgcgatgg actgctccag
     1921 aattagtaac cagctttcaa gacagactgc taactgcaga tcagactaag tatagtaata
     1981 tctggtctct gggtgtgaca ctttgggagc tttttgacaa tgccgcacag ccgtattcaa
     2041 acctttccaa cttagatgtc ctcaaccaag tcattagaga gagagacaca aaactcccga
     2101 agccccagct ggagcagccc tactctgata gatggtatga agtcttacag ttctgttggc
     2161 tgtcaccaga aaagagaccc gcggctgaag atgtgcacag gctgctgact tacctgcggc
     2221 tgcagagcca gcgggactca gaggtcgact ttgaacagca gtggaacgct ctgaagccga
     2281 acacaaacag cagagactcc tccaacaatg ctgcattccc aattctcgac cactttgcca
     2341 gggaccggct gggtcgtgaa atggaggaag tcctcaccgt gaccgaaacc agccagggcc
     2401 tgagcttcga gtatgtctgg gaggccgcta agcacgacca ctttgacgag cgcagccggg
     2461 gccacctgga cgaaggcttg tcctacacga gcatcttcta tccggttgaa gtttttgaga
     2521 gttcgctttc agatcctggg cccggaaagc aagatgacag cggccaggat gtccccctga
     2581 gggtccctgg agtggttcct gtttttgatg cccacaacct ttctgttgga agcgactatt
     2641 atatccagtt agaagaaaaa agtggtagta acttggagct tgattaccca ccagcgctgc
     2701 tcacaaccga catggataat ccagaaagga ctggccctga actgtcccag ctcacggcgc
     2761 tcaggagcgt tgaacttgag gagtccagta cagatgagga cttcttccaa agcagtacag
     2821 accccaaaga ctctagctta ccaggggact tacacgtgac cagtggcccc gagagccctt
     2881 tcaacaatat atttaatgat gtggacaaat cggaagattt gcccagtcac caaaaaatat
     2941 tcgacttaat ggaattaaac ggagttcaag ccgactttaa acctgccact ttaagttcca
     3001 gtttggataa ccccaaagag tcagtcataa caggccactt tgagaaagaa aagccccgta
     3061 agatttttga cagtgagcct ctctgcctat cagataatct tatgcaccaa gataattttg
     3121 atccattgaa tgttcaagaa ttgtcagaaa actttttatt tcttcaagag aaaaacttac
     3181 taaaaggctc attgtccagc aaagaacaca taaatgatct tcagacagaa cttaagaatg
     3241 ctggttttac tgaagctatg ttagaaacgt catgtagaaa ctctttagat actgagcttc
     3301 agtttgctga aaataagcca ggcttgtctt tgttgcagga aaacgtaagc acaaagggtg
     3361 acgatacaga tgtcatgctc acaggtgaca ctttgagcac ctcattgcag tcttccccgg
     3421 aagtgcaggt acctcctacc tccttcgaaa cagaagaaac gccccgtcgg gtacccccag
     3481 actcactccc aacacaggga gaaacccagc ccacgtgttt agatgttatt gtcccggagg
     3541 actgtctcca ccaggacatc agtccagacg ctgtgactgt cccggttgaa attctctcaa
     3601 ctgatgccag aacccacagc ctggataaca ggtcccagga ctctcctggc gagagtgagg
     3661 agaccctgcg actcaccgaa agtgactctg ttcttgctga tgacatcctt gccagcaggg
     3721 tgagtgtagg gagtagtctc ccggaactgg gacaggaatt gcacaataaa ccattttcgg
     3781 aagaccatca cagtcatcgc cggctagaga aaaacttaga ggctgtggag actttaaatc
     3841 agctcaattc taaagacgca gcaaaagaag caggcttggt gtctgccctc tcctcggact
     3901 caaccagtca ggacagcctc ctggaggaca gcttgtcagc acccttccca gcctctgagc
     3961 cgtccctgga aaccccggac tctctggagt cagtggatgt ccacgaagcg ctactggact
     4021 ctttaggatc tcacactccc cagaaactag tgccccccga taagccggca gacagtggct
     4081 acgaaacaga gaacttggag tctcccgagt ggaccttgca tcccgctccc gagggcaccg
     4141 cagactcaga accagccacc acgggcgatg gcggccacag cggtctgcct cccaacccgg
     4201 tcattgtcat ctcagatgcc ggcgatggtc acagaggcac agaagtgacc cctgagacgt
     4261 tcacagctgg ctcccagggt tcataccgag actctgcgta cttctcagac aatgactctg
     4321 agcccgagaa aaggtctgag gaggtcccgg gaacctcccc atccgccttg gtgttggtac
     4381 aggagcagcc cctacccgag ccagtcctcc ccgagcaaag tcctgctgcc caggatagct
     4441 gcctggaagc cagaaagagc cagccagatg aaagttgtct gtctgctttg cacaactcca
     4501 gtgacctgga attaagagcc acgccggagc cagcacagac tggtgttccc cagcaggtgc
     4561 atcccacgga agacgaggcc agcagtccct ggagtgtgct gaatgcagaa cttagcagcg
     4621 gcgatgactt cgagacacag gacgatcgcc cctgcaccct cgcttccacg gggaccaaca
     4681 cgaacgaact ccttgcctac accaattctg cgctggacaa gtccctgtcc agccactccg
     4741 agggcccgaa gttgaaggag ccggacatcg aagggaagta cctggggaaa ctcggggtgt
     4801 cagggatgct cgacctctca gaggacggga tggatgcaga cgaggaggac gaaaacagcg
     4861 acgactcgga cgaggacctg cgggccttca acctgcatag cctcagctcc gagtcggagg
     4921 acgagaccga gcaccccgtg cccatcatcc tcagcaacga ggacggaagg cacctgcgga
     4981 gtctgttgaa gcccacagcg gccaatgccc ccgacccact gcccgaggac tggaagaagg
     5041 aaaagaaggc agtcacgttt ttcgatgatg tcacagtcta cctgtttgac caggagaccc
     5101 caaccaaaga gctggggccc tgtggaggag aggcgtgcgg cccggacctg agcggcccag
     5161 ccccagcctc aggctctccc tacctgagca ggtgcatcaa ctccgaaagc tccaccgacg
     5221 aagaaggtgg tggctttgag tgggatgatg acttctcccc agatcctttt atgtcaaaga
     5281 caacaagtaa cctgctcagc tccaagcctt ctctccaaac atccaagtac ttttctccgc
     5341 cgccaccggc ccggagcacg gagcagagct ggccgcactc ggcgccttac tcccggttct
     5401 ccatctctcc cgccaacatt gccagctttt ccctcacaca cctgaccgac tcggacatcg
     5461 agcagggcgg aagcagcgaa gacggagaaa aggactaggg cctcatgggc ccagctttct
     5521 tgtacaaagt ggttgatatc aaggccgccg acatcaccag cctgtacaag aaagtggggt
     5581 ggagccatcc ccagttcgag aagggcggag gatctggcgg cggaagcggc ggaggaagct
     5641 ggtcccaccc tcagtttgag aaaggcaccg agctgggcag caccatggcc agctacccct
     5701 acgacgtgcc cgactacgcc aaggacaaca ccgtgcccct gaagctgatc gccctgctgg
     5761 ccaacggcga gttccactct ggcgagcagc tgggcgagac actgggcatg agcagagccg
     5821 ccatcaacaa gcacatccag accctgcggg actggggcgt ggacgtgttc acagtgcccg
     5881 gcaagggcta cagcctgccc gagcccatcc agctgctgaa cgccaagcag atcctgggcc
     5941 agctggatgg cggatctgtg gccgtgctgc ccgtgatcga cagcaccaac cagtatctgc
     6001 tcgaccggat cggcgagctg aagtccggcg acgcctgtat cgccgagtac cagcaggccg
     6061 gcagaggcgg caggggcaga aagtggttca gccccttcgg cgccaacctg tacctgagca
     6121 tgttctggag actggaacag ggcccagccg ccgctattgg cctgagcctg gtcatcggca
     6181 tcgtgatggc cgaggtgctg cggaagctgg gcgccgacaa agtgcgcgtg aagtggccca
     6241 acgacctgta tctgcaggac cggaagctgg ccggcatcct ggtggaactg accggcaaaa
     6301 caggcgacgc cgcccagatc gtgatcggcg ctggcatcaa catggccatg cggcgggtgg
     6361 aagagagcgt ggtcaaccag ggctggatca ccctgcagga agccggcatc aacctggacc
     6421 ggaacaccct ggccgccatg ctgatcagag agctgagagc cgccctggaa ctgttcgagc
     6481 aggaaggcct ggccccctac ctgagcagat gggagaagct ggacaacttc atcaaccggc
     6541 ccgtgaaact gatcatcggc gacaaagaga tcttcggcat cagccggggc atcgacaagc
     6601 agggggccct gctcctggaa caggacggca tcatcaagcc ctggatgggc ggcgagatca
     6661 gcctgcggag cgccgagaag tgatgactcg agtctagagg gcccgtttaa acccgctgat
     6721 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6781 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6841 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6901 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6961 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     7021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     7081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     7141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     7201 ccaaaaaact tgattagggt gatggttcac gtacctagaa gttcctattc cgaagttcct
     7261 attctctaga aagtatagga acttccttgg ccaaaaagcc tgaactcacc gcgacgtctg
     7321 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     7381 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     7441 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     7501 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     7561 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     7621 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     7681 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     7741 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     7801 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     7861 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     7921 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     7981 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     8041 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     8101 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     8161 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     8221 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     8281 gacgccccag cactcgtccg agggcaaagg aatagcacgt actacgagat ttcgattcca
     8341 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     8401 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     8461 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8521 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     8581 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8641 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     8701 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     8761 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8821 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8881 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8941 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9001 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9061 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9121 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9181 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9241 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9301 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     9361 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     9421 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     9481 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     9541 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     9601 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     9661 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     9721 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9781 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9841 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9901 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9961 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10021 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10081 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10141 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10201 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10261 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10321 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    10381 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    10441 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    10501 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    10561 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    10621 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    10681 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    10741 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    10801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    10861 tgc
//
