LOCUS       sequence-370483-        8442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human FGFR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_FGFR2
FEATURES             Location/Qualifiers
     source          1..8442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(31..48)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(202..790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(282..301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(961..1752,1753..1821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1584..1603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1822..1926)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2001..2020)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2192..2571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2572..2775
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2726..2746
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2777..2795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2798..2816
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2826..2850
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2993..3017
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5136..5160)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5207..5230
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5267..5290
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5321..5347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5321..5347
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5348..5695,5696..5698,5699..6307)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6351..6368)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6357..6581
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6714..6733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6865..6912
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6920..7942
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6920..7942
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8072..8193
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8109..8128)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8163..8182
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8242..8258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8242..8258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8255..8277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8266..8282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8290..8296,8297..8314,8315..8320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8335..8356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
       61 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      121 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      181 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      241 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      301 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      361 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      421 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      481 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      541 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      601 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      661 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      721 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      781 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      961 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1021 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1081 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1141 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1201 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1261 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1321 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1381 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1441 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1501 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1561 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1621 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1681 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1741 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1801 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1861 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1921 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     1981 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     2041 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     2101 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     2161 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     2221 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2281 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     2341 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     2401 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     2461 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     2521 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     2581 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     2641 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     2701 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     2761 tatataagca gagctctccc tatcagtgat agagatctcc ctatcagtga tagagatcgt
     2821 cgacgagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2881 ctccatagaa gacaccggga ccgatccagc ctccggactc tagcgtttaa acttaagctt
     2941 ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat caacaagttt
     3001 gtacaaaaaa gcaggctcca ccatggtcag ctggggtcgt ttcatctgcc tggtcgtggt
     3061 caccatggca accttgtccc tggcccggcc ctccttcagt ttagttgagg ataccacatt
     3121 agagccagaa ggagcaccat actggaccaa cacagaaaag atggaaaagc ggctccatgc
     3181 tgtgcctgcg gccaacactg tcaagtttcg ctgcccagcc ggggggaacc caatgccaac
     3241 catgcggtgg ctgaaaaacg ggaaggagtt taagcaggag catcgcattg gaggctacaa
     3301 ggtacgaaac cagcactgga gcctcattat ggaaagtgtg gtcccatctg acaagggaaa
     3361 ttatacctgt gtggtggaga atgaatacgg gtccatcaat cacacgtacc acctggatgt
     3421 tgtggagcga tcgcctcacc ggcccatcct ccaagccgga ctgccggcaa atgcctccac
     3481 agtggtcgga ggagacgtag agtttgtctg caaggtttac agtgatgccc agccccacat
     3541 ccagtggatc aagcacgtgg aaaagaacgg cagtaaatac gggcccgacg ggctgcccta
     3601 cctcaaggtt ctcaaggccg ccggtgttaa caccacggac aaagagattg aggttctcta
     3661 tattcggaat gtaacttttg aggacgctgg ggaatatacg tgcttggcgg gtaattctat
     3721 tgggatatcc tttcactctg catggttgac agttctgcca gcgcctggaa gagaaaagga
     3781 gattacagct tccccagact acctggagat agccatttac tgcatagggg tcttcttaat
     3841 cgcctgtatg gtggtaacag tcatcctgtg ccgaatgaag aacacgacca agaagccaga
     3901 cttcagcagc cagccggctg tgcacaagct gaccaaacgt atccccctgc ggagacaggt
     3961 ttcggctgag tccagctcct ccatgaactc caacaccccg ctggtgagga taacaacacg
     4021 cctctcttca acggcagaca cccccatgct ggcaggggtc tccgagtatg aacttccaga
     4081 ggacccaaaa tgggagtttc caagagataa gctgacactg ggcaagcccc tgggagaagg
     4141 ttgctttggg caagtggtca tggcggaagc agtgggaatt gacaaagaca agcccaagga
     4201 ggcggtcacc gtggccgtga agatgttgaa agatgatgcc acagagaaag acctttctga
     4261 tctggtgtca gagatggaga tgatgaagat gattgggaaa cacaagaata tcataaatct
     4321 tcttggagcc tgcacacagg atgggcctct ctatgtcata gttgagtatg cctctaaagg
     4381 caacctccga gaatacctcc gagcccggag gccacccggg atggagtact cctatgacat
     4441 taaccgtgtt cctgaggagc agatgacctt caaggacttg gtgtcatgca cctaccagct
     4501 ggccagaggc atggagtact tggcttccca aaaatgtatt catcgagatt tagcagccag
     4561 aaatgttttg gtaacagaaa acaatgtgat gaaaatagca gactttggac tcgccagaga
     4621 tatcaacaat atagactatt acaaaaagac caccaatggg cggcttccag tcaagtggat
     4681 ggctccagaa gccctgtttg atagagtata cactcatcag agtgatgtct ggtccttcgg
     4741 ggtgttaatg tgggagatct tcactttagg gggctcgccc tacccaggga ttcccgtgga
     4801 ggaacttttt aagctgctga aggaaggaca cagaatggat aagccagcca actgcaccaa
     4861 cgaactgtac atgatgatga gggactgttg gcatgcagtg ccctcccaga gaccaacgtt
     4921 caagcagttg gtagaagact tggatcgaat tctcactctc acaaccaatg aggaatactt
     4981 ggacctcagc caacctctcg aacagtattc acctagttac cctgacacaa gaagttcttg
     5041 ttcttcagga gatgattctg ttttttctcc agaccccatg ccttacgaac catgccttcc
     5101 tcagtatcca cacataaacg gcagtgttaa aacagaccca gctttcttgt acaaagtggt
     5161 tgatatcaag gccgccgaca tcaccagcct gtacaagaaa gtggggtgga gccatcccca
     5221 gttcgagaag ggcggaggat ctggcggcgg aagcggcgga ggaagctggt cccaccctca
     5281 gtttgagaaa ggcaccgagc tgggcagcac catggccagc tacccctacg acgtgcccga
     5341 ctacgccaag gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt
     5401 ccactctggc gagcagctgg gcgagacact gggcatgagc agagccgcca tcaacaagca
     5461 catccagacc ctgcgggact ggggcgtgga cgtgttcaca gtgcccggca agggctacag
     5521 cctgcccgag cccatccagc tgctgaacgc caagcagatc ctgggccagc tggatggcgg
     5581 atctgtggcc gtgctgcccg tgatcgacag caccaaccag tatctgctcg accggatcgg
     5641 cgagctgaag tccggcgacg cctgtatcgc cgagtaccag caggccggca gaggcggcag
     5701 gggcagaaag tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggagact
     5761 ggaacagggc ccagccgccg ctattggcct gagcctggtc atcggcatcg tgatggccga
     5821 ggtgctgcgg aagctgggcg ccgacaaagt gcgcgtgaag tggcccaacg acctgtatct
     5881 gcaggaccgg aagctggccg gcatcctggt ggaactgacc ggcaaaacag gcgacgccgc
     5941 ccagatcgtg atcggcgctg gcatcaacat ggccatgcgg cgggtggaag agagcgtggt
     6001 caaccagggc tggatcaccc tgcaggaagc cggcatcaac ctggaccgga acaccctggc
     6061 cgccatgctg atcagagagc tgagagccgc cctggaactg ttcgagcagg aaggcctggc
     6121 cccctacctg agcagatggg agaagctgga caacttcatc aaccggcccg tgaaactgat
     6181 catcggcgac aaagagatct tcggcatcag ccggggcatc gacaagcagg gggccctgct
     6241 cctggaacag gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc
     6301 cgagaagtga tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     6361 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6421 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6481 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6541 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6601 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6661 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6721 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6781 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6841 ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt ctctagaaag
     6901 tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     6961 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     7021 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     7081 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     7141 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     7201 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     7261 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     7321 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     7381 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     7441 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     7501 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     7561 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     7621 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     7681 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     7741 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     7801 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     7861 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     7921 tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg ccgccttcta
     7981 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8041 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8101 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8161 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8221 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8281 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8341 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8401 gtgccagctg cattaatgaa tcggccaacg cgcggggaga gg
//
