LOCUS       sequence-370484-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry 
            fluorescent marker for expression in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Lue NZ, Garcia EM, Ngan KC, Lee C, Doench JG, Liau BB
  TITLE     Base editor scanning charts the DNMT3A activity landscape.
  JOURNAL   Nat Chem Biol. 2023 Feb;19(2):176-186. doi: 
            10.1038/s41589-022-01167-4. Epub 2022 Oct 20.
  PUBMED    36266353
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-022-01167-4"; journalName: "Nat Chem Biol"; date: 
            "2023-02"; volume: "19"; issue: "2"; pages: "176-186"
COMMENT     Sequence Label: pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..284
                     /label=chicken beta-actin promoter
     intron          285..1301
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1225..1247
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1309..1328
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1356..1365
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1365..1388
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2523..3455
                     /codon_start=1
                     /product="catalytic domain of DNA methyltransferase 3A"
                     /label=DNMT3A catalytic domain
                     /translation="PSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATG
                     LLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVI
                     GGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVS
                     DKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGR
                     IAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSR
                     LARQRLLGRSWSVPVIRHLFAPLKEYFACV"
     misc_feature    join(3482..4056,4057..4059,4060..4068)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3649..3666)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3876..3895
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      4078..4087
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4084..4794
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4229..4247)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4497..4516
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4843..4861)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5135..5256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5172..5191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5226..5245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   5468..5530
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5581..5601)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5581..5599)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5620..5636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5620..5636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5633..5655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5644..5660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5668..5674,5675..5692,5693..5698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5713..5734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5851..5868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6102..6121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6781..7572,7573..7641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7404..7423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7642..7746)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7772..8227)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7909..7930)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8121..8140
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8354..8376
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8368..8385
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8369..8385
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8395..8413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8443..8477
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
       61 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      121 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
      181 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
      241 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct
      301 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga
      361 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc
      421 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc
      481 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
      541 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg
      601 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
      661 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
      721 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg
      781 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg
      841 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
      901 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg
      961 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     1021 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     1081 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc
     1141 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc
     1201 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
     1261 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
     1321 ttattgtgct gtctcatcat tttggcaaag aattcgccac catggactac aaggatgacg
     1381 acgataagat gaatgctgtg gaagagaacc aggcctctgg agagtctcag aaggtggagg
     1441 aggccagccc tcctgctgtg cagcagccca cggaccctgc ttctccgact gtggccacca
     1501 cccctgagcc agtaggaggg gatgctgggg acaagaatgc taccaaagca gccgacgatg
     1561 agcctgagta tgaggatggc cggggctttg gcattggaga gctggtgtgg gggaaacttc
     1621 ggggcttctc ctggtggcca ggccgaattg tgtcttggtg gatgacaggc cggagccgag
     1681 cagctgaagg cactcgctgg gtcatgtggt tcggagatgg caagttctca gtggtgtgtg
     1741 tggagaagct catgccgctg agctccttct gcagtgcatt ccaccaggcc acctacaaca
     1801 agcagcccat gtaccgcaaa gccatctacg aagtcctcca ggtggccagc agccgtgccg
     1861 ggaagctgtt tccagcttgc catgacagtg atgaaagtga cagtggcaag gctgtggaag
     1921 tgcagaacaa gcagatgatt gaatgggccc tcggtggctt ccagccctcg ggtcctaagg
     1981 gcctggagcc accagaagaa gagaagaatc cttacaagga agtttacacc gacatgtggg
     2041 tggagcctga agcagctgct tacgccccac ccccaccagc caagaaaccc agaaagagca
     2101 caacagagaa acctaaggtc aaggagatca ttgatgagcg cacaagggag cggctggtgt
     2161 atgaggtgcg ccagaagtgc agaaacatcg aggacatttg tatctcatgt gggagcctca
     2221 atgtcaccct ggagcaccca ctcttcattg gaggcatgtg ccagaactgt aagaactgct
     2281 tcttggagtg tgcttaccag tatgacgacg atgggtacca gtcctattgc accatctgct
     2341 gtggggggcg tgaagtgctc atgtgtggga acaacaactg ctgcaggtgc ttttgtgtcg
     2401 agtgtgtgga tctcttggtg gggccaggag ctgctcaggc agccattaag gaagacccct
     2461 ggaactgcta catgtgcggg cataagggca cctatgggct gctgcgaaga cgggaagact
     2521 ggccttctcg actccagatg ttctttgcca ataaccatga ccaggaattt gaccccccaa
     2581 aggtttaccc acctgtgcca gctgagaaga ggaagcccat ccgcgtgctg tctctctttg
     2641 atgggattgc tacagggctc ctggtgctga aggacctggg catccaagtg gaccgctaca
     2701 ttgcctccga ggtgtgtgag gactccatca cggtgggcat ggtgcggcac cagggaaaga
     2761 tcatgtacgt cggggacgtc cgcagcgtca cacagaagca tatccaggag tggggcccat
     2821 tcgacctggt gattggaggc agtccctgca atgacctctc cattgtcaac cctgcccgca
     2881 agggacttta tgagggtact ggccgcctct tctttgagtt ctaccgcctc ctgcatgatg
     2941 cgcggcccaa ggagggagat gatcgcccct tcttctggct ctttgagaat gtggtggcca
     3001 tgggcgttag tgacaagagg gacatctcgc gatttcttga gtctaacccc gtgatgattg
     3061 acgccaaaga agtgtctgct gcacacaggg cccgttactt ctggggtaac cttcctggca
     3121 tgaacaggcc tttggcatcc actgtgaatg ataagctgga gctgcaagag tgtctggagc
     3181 acggcagaat agccaagttc agcaaagtga ggaccattac caccaggtca aactctataa
     3241 agcagggcaa agaccagcat ttccccgtct tcatgaacga gaaggaggac atcctgtggt
     3301 gcactgaaat ggaaagggtg tttggcttcc ccgtccacta cacagacgtc tccaacatga
     3361 gccgcttggc gaggcagaga ctgctgggcc gatcgtggag cgtgccggtc atccgccacc
     3421 tcttcgctcc gctgaaggaa tattttgctt gtgtgtaaat gcatgagtaa ctgaggatcc
     3481 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     3541 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3601 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     3661 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     3721 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     3781 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3841 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3901 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3961 acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     4021 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccct ggaattcgcc
     4081 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     4141 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     4201 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     4261 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     4321 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     4381 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     4441 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4501 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4561 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4621 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4681 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4741 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagtcgac
     4801 ctcgaggggg ggcccggtac caggtaagtg tacccaattc gccctatagt gagtcgtatt
     4861 acaattcact cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggagatcca
     4921 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattca
     4981 cagtcccaag gctcatttca ggcccctcag tcctcacagt ctgttcatga tcataatcag
     5041 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     5101 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     5161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     5221 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgtgagat
     5281 cctcgagcta gttaaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt
     5341 ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt
     5401 aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga
     5461 taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat
     5521 ctttctaggg ttaatctagt atacgcgtat gcggccgctt aattaatcca gcttttgttc
     5581 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5641 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5701 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5761 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6421 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7081 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7741 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     7801 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7861 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     7921 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7981 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8041 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8101 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8161 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8221 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     8281 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     8341 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     8401 gactcactat agggcgaatt ggggcgcgcc attctagatt aaccctagaa agatagtctg
     8461 cgtaaaattg acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc
     8521 gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc
     8581 aatgcggtaa gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg
     8641 attatctttt acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg
     8701 ttctacttac gtgataactt attatatata tattttcttg ttatagatat caactagaat
     8761 gctagcatgg gcccagatct
//
