LOCUS       sequence-37053-e        6704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD4d3+4-bio
FEATURES             Location/Qualifiers
     source          1..6704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    799..854
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(853..872)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3090..3108)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3176..3280
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3281..3349,3350..4141)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3499..3518)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4312..4900
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4801..4820
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5054..5071
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5188..5209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5224..5229,5230..5247,5248..5254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5262..5278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5267..5289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5286..5302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5286..5302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        5573..5952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5953..6156
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6107..6127
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6153..6177
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     6658..6677
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gcggccgctc cggctagcga taagcttgac tcagatcccc atccgctcaa gcaggccacc
       61 atgtgccgag gcttctcttt caggcacttg ctgccgctgc tgctcctgca gctgtcaaaa
      121 ctcctagttg tcacccaagg gtcgacatcc atcacggcct ataagagtga gggggagtca
      181 gcggagttct ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg
      241 aaggcagaga aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag
      301 gtgtctgtgc agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc
      361 acccttcaga taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact
      421 ctggacagag ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc
      481 gacagcaaca ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc
      541 ttgaagcagg agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag
      601 gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac
      661 tccaagatcc aggttttatc caaagggttg aattccggat cactgcatca tattctggat
      721 gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac gtgattaatt aaggatcccc
      781 cgacctcgac ctctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
      841 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttggtc gagatccctc
      901 ggagatctct agctagagga tcgatccccg ccccggacga actaaacctg actacgacat
      961 ctctgcccct tcttcgcggg gcagtgcatg taatcccttc agttggttgg tacaacttgc
     1021 caactgggcc ctgttccaca tgtgacacgg ggggggacca aacacaaagg ggttctctga
     1081 ctgtagttga catccttata aatggatgtg cacatttgcc aacactgagt ggctttcatc
     1141 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     1201 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     1261 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     1321 aagagggcat tagcaatagt gtttataagg cccccttgtt aaccctaaac gggtagcata
     1381 tgcttcccgg gtagtagtat atactatcca gactaaccct aattcaatag catatgttac
     1441 ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa gggtcctaag gaacagcgat
     1501 atctcccacc ccatgagctg tcacggtttt atttacatgg ggtcaggatt ccacgagggt
     1561 agtgaaccat tttagtcaca agggcagtgg ctgaagatca aggagcgggc agtgaactct
     1621 cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc taatagaata actgctgagt
     1681 tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga
     1741 ataaaatttg gacggggggt tcagtggtgg cattgtgcta tgacaccaat ataaccctca
     1801 caaacccctt gggcaataaa tactagtgta ggaatgaaac attctgaata tctttaacaa
     1861 tagaaatcca tggggtgggg acaagccgta aagactggat gtccatctca cacgaattta
     1921 tggctatggg caacacataa tcctagtgca atatgatact ggggttatta agatgtgtcc
     1981 caggcaggga ccaagacagg tgaaccatgt tgttacactc tatttgtaac aaggggaaag
     2041 agagtggacg ccgacagcag cggactccac tggttgtctc taacaccccc gaaaattaaa
     2101 cggggctcca cgccaatggg gcccataaac aaagacaagt ggccactctt ttttttgaaa
     2161 ttgtggagtg ggggcacgcg tcagccccca cacgccgccc tgcggttttg gactgtaaaa
     2221 taagggtgta ataacttggc tgattgtaac cccgctaacc actgcggtca aaccacttgc
     2281 ccacaaaacc actaatggca ccccggggaa tacctgcata agtaggtggg cgggccaaga
     2341 taggggcgcg attgctgcga tctggaggac aaattacaca cacttgcgcc tgagcgccaa
     2401 gcacagggtt gttggtcctc atattcacga ggtcgctgag agcacggtgg gctaatgttg
     2461 ccatgggtag catatactac ccaaatatct ggatagcata tgctatccta atctatatct
     2521 gggtagcata ggctatccta atctatatct gggtagcata tgctatccta atctatatct
     2581 gggtagtata tgctatccta atttatatct gggtagcata ggctatccta atctatatct
     2641 gggtagcata tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc
     2701 gggtagcata tgctatccta atagagatta gggtagtata tgctatccta atttatatct
     2761 gggtagcata tactacccaa atatctggat agcatatgct atcctaatct atatctgggt
     2821 agcatatgct atcctaatct atatctgggt agcataggct atcctaatct atatctgggt
     2881 agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt
     2941 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
     3001 agtatatgct atcctaatct gtatccgggt agcatatgct atcctcatga taagctgtca
     3061 aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3121 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3181 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3241 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3301 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3361 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3421 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3481 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     3541 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3601 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3661 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3721 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3781 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac
     3841 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3901 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3961 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4021 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4081 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4141 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4201 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4261 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4321 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4381 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4441 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4501 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4561 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4621 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4681 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4741 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4801 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4861 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4921 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4981 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5041 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5101 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5161 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5221 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5281 tcacacagga aacagctatg accatgatta cgccaagctc tagctagagg tcgaccaatt
     5341 ctcatgtttg acagcttatc atcgcagatc cgggcaacgt tgttgccatt gctgcaggcg
     5401 cagaactggt aggtatggaa gatctataca ttgaatcaat attggcaatt agccatatta
     5461 gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
     5521 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga
     5581 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5641 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5701 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5761 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5821 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5881 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
     5941 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
     6001 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6061 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
     6121 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcctc
     6181 actctcttcc gcatcgctgt ctgcgagggc cagctgttgg gctcgcggtt gaggacaaac
     6241 tcttcgcggt ctttccagta ctcttggatc ggaaacccgt cggcctccga acggtactcc
     6301 gccaccgagg gacctgagcg agtccgcatc gaccggatcg gaaaacctct cgagaaaggc
     6361 gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg gcgggcggca gcgggtggcg
     6421 gtcggggttg tttctggcgg aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag
     6481 acggcggatg gtcgaggtga ggtgtggcag gcttgagatc cagctgttgg ggtgagtact
     6541 ccctctcaaa agcgggcatt acttctgcgc taagattgtc agtttccaaa aacgaggagg
     6601 atttgatatt cacctggccc gatctggcca tacacttgag tgacaatgac atccactttg
     6661 cctttctctc cacaggtgtc cactcccagg tccaagttta aact
//
