LOCUS       sequence-37054-e         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F2-ATG
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     44..64
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(384..403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(413..430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(413..429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
       61 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagattac ttttcacctg
      121 tatggatttt ggtgtgacgc tgcaggtcgc tggactgggc aaacttcctc ccacagatgt
      181 cgcatgcaaa aggcttctcg ccggtgtgtg tgcggatgtg ctgggtgaga gcgtcagaac
      241 ggctgaagtt cctcatgcag atgcgacact ggaagggctt ctggcctgtg tgaatccgga
      301 tatgctgggt caggtggtcg gaccgagaga agcggcggtc gcaggactcg acagggcaag
      361 catatgggcg ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
      421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccg
//
