LOCUS       sequence-37057-e         725 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F3-GTA
FEATURES             Location/Qualifiers
     source          1..725
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     44..64
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(384..403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(413..430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(413..429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(658..677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
       61 aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagattat ttctcacccg
      121 tgtggatctt ggtgtgacgg gtcagagcac cagactgcgc gaacttcctg ccgcagatgt
      181 cgcatgcaaa aggcttctcg cctgtgtggg tgcggatgtg ggtggtaagg tggtcactac
      241 gactgaagtt acgcatacag attcgacact ggaagggctt ctgaccggta tggatgcgga
      301 tatggcgggt aagctcatcc gagcgagaaa agcggcgatc gcaggactcg acagggcaag
      361 catatgggcg ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
      421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      721 atcgg
//
