LOCUS       sequence-370611-        4151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EdH4gp0214 deletion HR vector 250bp HR Arms (EdH4 phage editing).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Adler BA, Hessler T, Cress BF, Lahiri A, Mutalik VK, Barrangou R, 
            Banfield J, Doudna JA
  TITLE     Broad-spectrum CRISPR-Cas13a enables efficient phage genome editing.
  JOURNAL   Nat Microbiol. 2022 Dec;7(12):1967-1979. doi: 
            10.1038/s41564-022-01258-x. Epub 2022 Oct 31.
  PUBMED    36316451
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01258-x"; journalName: "Nat Microbiol"; date: 
            "2022-12"; volume: "7"; issue: "12"; pages: "1967-1979"
COMMENT     Sequence Label: pBA1031
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(667..1482)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(667..1482)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1390..1409
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1474..1493
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(3506..4151,1..17)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVLAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cgctgccgcg ccggtagcac ttgggttgcg cagcaacccg taagtgcgct gttccagact
       61 atccacctgc ccgaaccaga gacttatcaa gaagctgcaa tgcaggctgc aggtgtggat
      121 gaaaactatg tagctatgtg catgtgccga gatcaggttg ttctgtataa agcgtatcga
      181 ccaaacggag ccacggagtg gcttgtattc cgacccgaag gatatgaaat cttagaggca
      241 ctatgataaa tcttgccaag gttgcaacga cactgttaat tatttgctgg tctgtaacct
      301 taacactgtg tcttgtattt cagtgaggtg cttgtatgga taagagacgg ctcaacgccc
      361 gtctcacagc tgacagtata gtgactaaca agaggtgagg agtgactgat tttactgaag
      421 ctttaatgat ggccttgtct gttttagcgc tatcggtgtg ttttggggcg ttattcacat
      481 ctctggttaa agttttacat acagtattat tacccaagga gcgcagtaat gacgaagatt
      541 gacttgaatg ctggcgacca tgttgttgtc aaatgcatcg gagattatct tggattcaca
      601 atcggtaata cttacgaggc aggaggactt tggcaggtgg tgttacaacc aattaaccaa
      661 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
      721 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
      781 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
      841 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
      901 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
      961 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     1021 tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     1081 aatcgaatgc aaccggcgca ggaacactgc aagcgcatca acaatatttt cacctgaatc
     1141 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     1201 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     1261 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     1321 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     1381 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     1441 tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     1501 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     1561 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
     1621 gaaggatcag cttgcgtttt cccttgtcca gatagcccag tagctgacat ttagcctaaa
     1681 tgacatttct ccggaaagtg cgcacggcca cgctcttgtt gacgttgagc ttgcttttga
     1741 cgttgaacct cgcgttgctc ggttaataat ttctcaccaa ccgctttaat aatggccgcc
     1801 gctttcgctt gcatgtcacg gttcagagga ccgagtgcat ctaataccgg tttcaaacgt
     1861 tcgcgtaaat cgcgcgcagt ttcctgtgcc tggtctgctt tcttacgcat ttcgcgggcg
     1921 ccggcagccg cttgcagtgc tggttcgtaa ccttgacgga ctgccttagt taagcgatcc
     1981 gcgactgcct caggggtttc cacacgttta gaaatgccta atttctccgc cagaccttgt
     2041 ggagcgtaag cacggggttc cacggcctga ggcgaaatcg taacatgtcc tacaggcggg
     2101 cgttcaagcg cttcataaaa tgcttggata cgagtatggc gcgctttact cccttcaatc
     2161 ccacgctgaa ggcccaggtc cgcaaccgca gcagcgaagg tcgtttgatc acgtgtcatt
     2221 tgggccttat taccaataaa ttctttagca ctcaagcgac catcttgggt caaggggact
     2281 acaaaggccg tcatatgtgg ggatgtctca tcgcgatgaa tcgaagctgt tacaatacgg
     2341 tcagcgccat atttatcggc taaccattta tgggctttct caaagaatgc tgcttgttgc
     2401 tcctgtgacg cgcttttcca ccactcgggc gaagcggtca tcacatattc tacggcaagt
     2461 actgcatctt tacggcgctt ttcaggcaac agttcacgca aacgacccat ggcctcgtcc
     2521 gtactactcg cggcccaatg ttcattttcc ggagtacgag atgcatccgc attcggtgtt
     2581 tcacgttcac gatacgcatg tttcagcgac gcagcaacat tacccatctt ggcgagtttc
     2641 ttacaacgca taattgcata agcggccatg cctgcccctc ccttttggtg tccaaccggc
     2701 tcgacggggg cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca
     2761 ctagactttg cttcgcaaag tcgtgaccgc ctacggcggc tgcgcctgcc cacgagcttg
     2821 accacaggga ttgcccaccg gctacccagc cttcgaccac atacccaccg gctccaactg
     2881 cgcggcctgc ggccttgccc catcaatttt tttaattttc tctggggaaa agcctccggc
     2941 ctgcggcctg cgcgcttcgc ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg
     3001 cgcagcgacc gcgcagcggc ttggccttga cgcgcctgga acgacccaag cctatgcgag
     3061 tgggggcagt cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
     3121 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
     3181 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
     3241 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
     3301 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
     3361 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
     3421 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
     3481 gcacccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc
     3541 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg
     3601 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg
     3661 ccaccagaac gccgtggtgg tcagccaaaa gacactttcc aagctcatcg gacgttcttt
     3721 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa
     3781 gctcaacggc cccggcaccg tgttggccta cgtggtcaat gaccgcgtgg cgtggggcca
     3841 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga
     3901 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg
     3961 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg
     4021 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca
     4081 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc
     4141 gacacgggtc a
//
