LOCUS       sequence-370613-        8805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human NTRK3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_NTRK3
FEATURES             Location/Qualifiers
     source          1..8805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1999..2018)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2190..2569
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2570..2773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2724..2744
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2775..2793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2796..2814
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2824..2848
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5497..5521)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5568..5591
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5628..5651
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5682..5708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             5682..5708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(5709..6056,6057..6059,6060..6668)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(6712..6729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6718..6942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7075..7094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7226..7273
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7281..8303
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7281..8303
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8433..8554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8470..8489)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8524..8543
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8603..8619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8603..8619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8616..8638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8627..8643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8651..8657,8658..8675,8676..8681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8696..8717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1981 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     2041 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     2101 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     2161 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     2221 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     2281 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     2341 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     2401 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     2461 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     2521 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     2581 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     2641 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     2701 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     2761 tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg
     2821 acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2881 ccatagaaga caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttgg
     2941 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     3001 acaaaaaagc aggctccacc atggatgtct ctctttgccc agccaagtgt agtttctggc
     3061 ggattttctt gctgggaagc gtctggctgg actatgtggg ctccgtgctg gcttgccctg
     3121 caaattgtgt ctgcagcaag actgagatca attgccggcg gccggacgat gggaacctct
     3181 tccccctcct ggaagggcag gattcaggga acagcaatgg gaacgccagt atcaacatca
     3241 cggacatctc aaggaatatc acttccatac acatagagaa ctggcgcagt cttcacacgc
     3301 tcaacgccgt ggacatggag ctctacaccg gacttcaaaa gctgaccatc aagaactcag
     3361 gacttcggag cattcagccc agagcctttg ccaagaaccc ccatttgcgt tatataaacc
     3421 tgtcaagtaa ccggctcacc acactctcgt ggcagctctt ccagacgctg agtcttcggg
     3481 aattgcagtt ggagcagaac tttttcaact gcagctgtga catccgctgg atgcagctct
     3541 ggcaggagca gggggaggcc aagctcaaca gccagaacct ctactgcatc aacgctgatg
     3601 gctcccagct tcctctcttc cgcatgaaca tcagtcagtg tgaccttcct gagatcagcg
     3661 tgagccacgt caacctgacc gtacgagagg gtgacaatgc tgttatcact tgcaatggct
     3721 ctggatcacc ccttcctgat gtggactgga tagtcactgg gctgcagtcc atcaacactc
     3781 accagaccaa tctgaactgg accaatgttc atgccatcaa cttgacgctg gtgaatgtga
     3841 cgagtgagga caatggcttc accctgacgt gcattgcaga gaacgtggtg ggcatgagca
     3901 atgccagtgt tgccctcact gtctactatc ccccacgtgt ggtgagcctg gaggagcctg
     3961 agctgcgcct ggagcactgc atcgagtttg tggtgcgtgg caacccccca ccaacgctgc
     4021 actggctgca caatgggcag cctctgcggg agtccaagat catccatgtg gaatactacc
     4081 aagagggaga gatttccgag ggctgcctgc tcttcaacaa gcccacccac tacaacaatg
     4141 gcaactatac cctcattgcc aaaaacccac tgggcacagc caaccagacc atcaatggcc
     4201 acttcctcaa ggagcccttt ccagagagca cggataactt tatcttgttt gacgaagtga
     4261 gtcccacacc tcctatcact gtgacccaca aaccagaaga agacactttt ggggtatcca
     4321 tagcagttgg acttgctgct tttgcctgtg tcctgttggt ggttctcttc gtcatgatca
     4381 acaaatatgg tcgacggtcc aaatttggaa tgaagggtcc cgtggctgtc atcagtggtg
     4441 aggaggactc agccagccca ctgcaccaca tcaaccacgg catcaccacg ccctcgtcac
     4501 tggatgccgg gcccgacact gtggtcattg gcatgactcg catccctgtc attgagaacc
     4561 cccagtactt ccgtcaggga cacaactgcc acaagccgga cacgtatgtg cagcacatta
     4621 agaggagaga catcgtgctg aagcgagaac tgggtgaggg agcctttgga aaggtcttcc
     4681 tggccgagtg ctacaacctc agcccgacca aggacaagat gcttgtggct gtgaaggccc
     4741 tgaaggatcc caccctggct gcccggaagg atttccagag ggaggccgag ctgctcacca
     4801 acctgcagca tgagcacatt gtcaagttct atggagtgtg cggcgatggg gaccccctca
     4861 tcatggtctt tgaatacatg aagcatggag acctgaataa gttcctcagg gcccatgggc
     4921 cagatgcaat gatccttgtg gatggacagc cacgccaggc caagggtgag ctggggctct
     4981 cccaaatgct ccacattgcc agtcagatcg cctcgggtat ggtgtacctg gcctcccagc
     5041 actttgtgca ccgagacctg gccaccagga actgcctggt tggagcgaat ctgctagtga
     5101 agattgggga cttcggcatg tccagagatg tctacagcac ggattattac agggtgggag
     5161 gacacaccat gctccccatt cgctggatgc ctcctgaaag catcatgtac cggaagttca
     5221 ctacagagag tgatgtatgg agcttcgggg tgatcctctg ggagatcttc acctatggaa
     5281 agcagccatg gttccaactc tcaaacacgg aggtcattga gtgcattacc caaggtcgtg
     5341 ttttggagcg gccccgagtc tgccccaaag aggtgtacga tgtcatgctg gggtgctggc
     5401 agagggaacc acagcagcgg ttgaacatca aggagatcta caaaatcctc catgctttgg
     5461 ggaaggccac cccaatctac ctggacattc ttggcgaccc agctttcttg tacaaagtgg
     5521 ttgatatcaa ggccgccgac atcaccagcc tgtacaagaa agtggggtgg agccatcccc
     5581 agttcgagaa gggcggagga tctggcggcg gaagcggcgg aggaagctgg tcccaccctc
     5641 agtttgagaa aggcaccgag ctgggcagca ccatggccag ctacccctac gacgtgcccg
     5701 actacgccaa ggacaacacc gtgcccctga agctgatcgc cctgctggcc aacggcgagt
     5761 tccactctgg cgagcagctg ggcgagacac tgggcatgag cagagccgcc atcaacaagc
     5821 acatccagac cctgcgggac tggggcgtgg acgtgttcac agtgcccggc aagggctaca
     5881 gcctgcccga gcccatccag ctgctgaacg ccaagcagat cctgggccag ctggatggcg
     5941 gatctgtggc cgtgctgccc gtgatcgaca gcaccaacca gtatctgctc gaccggatcg
     6001 gcgagctgaa gtccggcgac gcctgtatcg ccgagtacca gcaggccggc agaggcggca
     6061 ggggcagaaa gtggttcagc cccttcggcg ccaacctgta cctgagcatg ttctggagac
     6121 tggaacaggg cccagccgcc gctattggcc tgagcctggt catcggcatc gtgatggccg
     6181 aggtgctgcg gaagctgggc gccgacaaag tgcgcgtgaa gtggcccaac gacctgtatc
     6241 tgcaggaccg gaagctggcc ggcatcctgg tggaactgac cggcaaaaca ggcgacgccg
     6301 cccagatcgt gatcggcgct ggcatcaaca tggccatgcg gcgggtggaa gagagcgtgg
     6361 tcaaccaggg ctggatcacc ctgcaggaag ccggcatcaa cctggaccgg aacaccctgg
     6421 ccgccatgct gatcagagag ctgagagccg ccctggaact gttcgagcag gaaggcctgg
     6481 ccccctacct gagcagatgg gagaagctgg acaacttcat caaccggccc gtgaaactga
     6541 tcatcggcga caaagagatc ttcggcatca gccggggcat cgacaagcag ggggccctgc
     6601 tcctggaaca ggacggcatc atcaagccct ggatgggcgg cgagatcagc ctgcggagcg
     6661 ccgagaagtg atgactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     6721 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6781 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6841 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6901 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6961 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     7021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7141 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7201 attagggtga tggttcacgt acctagaagt tcctattccg aagttcctat tctctagaaa
     7261 gtataggaac ttccttggcc aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     7321 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     7381 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     7441 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     7501 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     7561 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     7621 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     7681 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     7741 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     7801 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     7861 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     7921 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     7981 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     8041 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     8101 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     8161 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     8221 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     8281 ctcgtccgag ggcaaaggaa tagcacgtac tacgagattt cgattccacc gccgccttct
     8341 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     8401 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     8461 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8521 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     8581 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8641 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8701 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8761 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcg
//
