LOCUS       sequence-370652-       12334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminal flag-tagged human CAD 3E@3R in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 12334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST flag-CAD 3E@3R
FEATURES             Location/Qualifiers
     source          1..12334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(8..582,583..585,586..594)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(175..192)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     402..421
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             595..990
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1161..1630
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1760..1777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1931..2519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2011..2030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2683..3474,3475..3543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3306..3325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3544..3648)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3678..4271
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4334..4691
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4750..5166
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5096..5118
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5134..5150
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5176..5550
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5488..5504
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      5577..5586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12254..12277
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
       61 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      121 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      181 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      241 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      301 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      361 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      421 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      481 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      541 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtt
      601 cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc
      661 cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc
      721 actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc
      781 actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac
      841 aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg
      901 atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg
      961 ctgccctctg gttatgtgtg ggagggctaa gtcgacgata aaataaaaga ttttatttag
     1021 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt
     1081 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag
     1141 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     1201 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc
     1261 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     1321 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     1381 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     1441 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     1501 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     1561 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     1621 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     1681 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1741 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1801 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2221 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2341 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg cggccggccg
     2641 caaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3661 aaaagtgcca cctgcataat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     3721 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagaaaagtt cagatcaagg
     3781 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3841 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     3901 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     3961 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     4021 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     4081 tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcctccg
     4141 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     4201 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     4261 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
     4321 accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta
     4381 tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga
     4441 cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg
     4501 acttcggggg ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg ttttggactc
     4561 tttggtgcac ccccctaata ggagggatat gtggttctgg taggagacga gaacctaaaa
     4621 cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt
     4681 cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
     4741 gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     4801 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     4861 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     4921 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     4981 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     5041 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     5101 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     5161 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
     5221 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
     5281 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
     5341 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
     5401 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
     5461 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
     5521 gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt aaggatccat
     5581 ggcggcccta gtgttggagg acgggtcggt cctgcggggc cagccctttg gggccgccgt
     5641 gtcgactgcc ggggaagtgg tgtttcaaac cggcatggtc ggctaccccg aggccctcac
     5701 tgatccctcc tacaaggcac agatcttagt gctcacctat cctctgatcg gcaactatgg
     5761 catcccccca gatgaaatgg atgagttcgg tctctgcaag tggtttgaat cctcgggcat
     5821 ccacgtagca gcactggtag tgggagagtg ctgtcctact cccagccact ggagtgccac
     5881 ccgcaccctg catgagtggc tgcagcagca tggcatccct ggcttgcaag gagtagacac
     5941 tcgggagctg accaagaagt tgcgggaaca ggggtctctg ctggggaagc tggtccagaa
     6001 tggaacagaa ccttcaagtc tgccattctt ggaccccaat gcccgccccc tggtaccaga
     6061 ggtctccatt aagactccac gggtattcaa tacagggggt gcccctcgga tccttgcttt
     6121 ggactgtggc ctcaagtata atcagatccg atgcctctgc cagcgtgggg ctgaggtcac
     6181 tgtggtaccc tgggaccatg cactagacag ccaagagtat gagggtctct tcttaagtaa
     6241 tgggcctggt gaccctgcct cctatcccag tgtcgtatcc acactgagcc gtgttttatc
     6301 tgagcctaat ccccgacctg tctttgggat ctgcctggga caccagctat tggccttagc
     6361 cattggggcc aagacttaca agatgagata tgggaaccga ggccataacc agccctgctt
     6421 gttggtgggc tctgggcgct gctttctgac atcccagaac catgggtttg ctgtggagac
     6481 agactcactg ccagcagact gggctcctct cttcaccaac gccaatgatg gttccaatga
     6541 aggcattgtg cacaacagct tgcctttctt cagtgtccag tttcacccag agcaccaagc
     6601 tggcccttca gatatggaac tgcttttcga tatctttctg gaaactgtga aagaggccac
     6661 agctgggaac cctgggggcc agacagttag agagcggctg actgagcgcc tctgtccccc
     6721 tgggattccc actcccggct ctggacttcc accaccacga aaggttctga tcctgggctc
     6781 agggggcctc tccattggcc aagctggaga atttgactac tcgggctctc aggcaattaa
     6841 ggccctgaag gaggaaaaca tccagacgtt gctgatcaac cccaatattg ccacagtgca
     6901 gacctcccag gggctggccg acaaggtcta ttttcttccc ataacacctc attatgtaac
     6961 ccaggtgata cgtaatgaac gccccgatgg tgtgttactg acttttgggg gccagactgc
     7021 tctgaactgt ggtgtggagc tgaccaaggc cggggtgctg gctcggtatg gggtccgggt
     7081 cctgggcaca ccagtggaga ccattgagct gaccgaggat cgacgggcct ttgctgccag
     7141 aatggcagag atcggagagc atgtggcccc gagcgaggca gcaaattctc ttgaacaggc
     7201 ccaggcagcc gctgaacggc tggggtaccc tgtgctagtg cgtgcagcct ttgccctggg
     7261 tggcctgggc tctggctttg cctctaacag ggaggagctc tctgctctcg tggccccagc
     7321 ttttgcccat accagccaag tgctagtaga caagtctctg aagggatgga aggagattga
     7381 gtacgaggtg gtgagagacg cctatggcaa ctgtgtcacg gtgtgtaaca tggagaactt
     7441 ggacccactg ggcatccaca ctggtgagtc catagtggtg gcccctagcc agacactgaa
     7501 tgacagggag tatcagctcc tgaggcagac agctatcaag gtgacccagc acctgggaat
     7561 tgttggggag tgcaatgtgc agtatgcctt gaaccctgag tctgagcagt attacatcat
     7621 tgaagtgaat gccaggctct ctcgcagctc tgccctggcc agtaaggcca caggttatcc
     7681 actggcttat gtggcagcca agctagcatt gggcatccct ttgcctgagc tcaggaactc
     7741 tgtgacaggg ggtacagcag cctttgaacc cagcgtggat tattgtgtgg tgaagattcc
     7801 tcgatgggac cttagcaagt tcctgcgagt cagcacaaag attgggagct gcatgaagag
     7861 cgttggtgaa gtcatgggca ttgggcgttc atttgaggag gccttccaga aggccctgcg
     7921 catggtggat gagaactgtg tgggctttga tcacacagtg aaaccagtca gcgatatgga
     7981 gttggagact ccaacagata agcggatttt tgtggtggca gctgctttgt gggctggtta
     8041 ttcagtggac cgcctgtatg agctcacacg catcgaccgc tggttcctgc accgaatgaa
     8101 gcgtatcatc gcacatgccc agctgctaga acaacaccgt ggacagcctt tgccgccaga
     8161 cctgctgcaa caggccaagt gtcttggctt ctcagacaaa cagattgccc ttgcagttct
     8221 gagcacagag ctggctgttc gcaagctgcg tcaggaactg gggatctgtc cagcagtgaa
     8281 acagattgac acagttgcag ctgagtggcc agcccagaca aattacctat acctaacgta
     8341 ttggggcacc acccatgacc tcacctttcg aacacctcat gtcctagtcc ttggctctgg
     8401 cgtctaccgt attggctcta gcgttgaatt tgactggtgt gctgtaggct gcatccagca
     8461 gctccgaaag atgggatata agaccatcat ggtgaactat aacccagaga cagtcagcac
     8521 cgactatgac atgtgtgatc gactctactt tgatgagatc tcttttgagg tggtgatgga
     8581 catctatgag ctcgagaacc ctgaaggtgt gatcctatcc atgggtggac agctgcccaa
     8641 caacatggcc atggcgttgc atcggcagca gtgccgggtg ctgggcacct cccctgaagc
     8701 cattgactcg gctgagaacc gtttcaagtt ttcccggctc cttgacacca ttggtatcag
     8761 ccagcctcag tggagggagc tcagtgacct cgagtctgct cgccaattct gccagaccgt
     8821 ggggtacccc tgtgtggtgc gcccctccta tgtgctgagc ggtgctgcta tgaatgtggc
     8881 ctacacggat ggagacctgg agcgcttcct gagcagcgca gcagccgtct ccaaagagca
     8941 tcccgtggtc atctccaagt tcatccagga ggctaaggag attgacgtgg atgccgtggc
     9001 ctctgatggt gtggtggcag ccatcgccat ctctgagcat gtggagaatg caggtgtgca
     9061 ttcaggtgat gcgacgctgg tgaccccccc acaagatatc actgccaaaa ccctggagcg
     9121 gatcaaagcc attgtgcatg ctgtgggcca ggagctacag gtcacaggac ccttcaatct
     9181 gcagctcatt gccaaggatg accagctgaa agttattgaa tgcaacgtac gtgtctctcg
     9241 ctccttcccc ttcgtttcca agacactggg tgtggaccta gtagccttgg ccacgcgggt
     9301 catcatgggg gaagaagtgg aacctgtggg gctaatgact ggttctggag ttgtgggagt
     9361 aaaggtgcct cagttctcct tctcccgctt ggcgggtgct gacgtggtgt tgggtgtgga
     9421 aatgaccagt actggggagg tggccggctt tggggagagc cgctgtgagg catacctcaa
     9481 ggccatgcta agcactggct ttaagatccc caagaagaat atcctgctga ccattggcag
     9541 ctataagaac aaaagcgagc tgctcccaac tgtgcggcta ctggagagcc tgggctacag
     9601 cctctatgcc agtctcggca cagctgactt ctacactgag catggcgtca aggtaacagc
     9661 tgtggactgg cactttagga gggctgtgga tggtaggtgc ccaccacagc ggagcatcct
     9721 ggagcagcta gctgagaaaa actttgagct ggtgattaac ctgtcaatgg agggagctgg
     9781 gggcgaagag ctctcttcct ttgtcaccaa gggctaccgc acccgacgct tggccgctga
     9841 cttctccgtg cccctaatca tcgatatcaa gtgcaccaaa ctctttgtgg aggccctagg
     9901 ccagatcggg ccagcccctc ctttgaaggt gcatgttgac tgtatgacct cccaaaagct
     9961 tgtgcgactg ccgggattga ttgatgtcca tgtgcacctg cgggaaccag gtgggacaca
    10021 taaggaggac tttgcttcag gcacagccgc tgccctggct gggggtatca ccatggtgtg
    10081 tgccatgcct aatacccggc cccccatcat tgacgcccct gctctggccc tggcccagaa
    10141 gctggcagag gctggcgccc ggtgcgactt tgcgctattc cttggggcct cgtctgaaaa
    10201 tgcaggaacc ttgggcaccg tggccgggtc tgcagccggg ctgaagcttt acctcaatga
    10261 gaccttctct gagctgcggc tggacagcgt ggtccagtgg atggagcatt tcgagacatg
    10321 gccctcccac ctccccattg tggctcacgc ggagcagcaa accgtggctg ctgtcctcat
    10381 ggtggctcag ctcactcagc gctcagtgca catatgtcac gtggcacgga aggaggagat
    10441 cctgctaatt aaagctgcaa aggcacgggg cttgccagtg acctgcgagg tggctcccca
    10501 ccacctgttc ctaagccatg atgacctgga gcgcctgggg cctgggaagg gggaggtccg
    10561 gcctgagctt ggctcccgcc aggatgtgga agccctgtgg gagaacatgg ctgtcatcga
    10621 ctgctttgcc tcagaccatg ctccccatac cttggaggag aagtgtgggt ccaggccccc
    10681 acctgggttc ccagggttag agaccatgct gccactactc ctgacggctg taagcgaggg
    10741 ccggctcagc ctggacgacc tgctgcagcg attgcaccac aatcctcggc gcatctttca
    10801 cctgcctccg caggaggaca cctatgtgga ggtggatctg gagcatgagt ggacaattcc
    10861 cagccacatg cccttctcca aggcccactg gacacctttt gaagggcaga aagtgaaggg
    10921 caccgtccgc cgtgtggtcc tgcgagggga ggttgcctat atcgatgggc aggttctggt
    10981 acccccgggc tatggacagg atgtacggaa gtggccacag ggggctgttc ctcagctccc
    11041 accctcagcc cctgccacta gtgagatgac cacgacacct gaaagacccc gccgtggcat
    11101 cccagggctt cctgatggcc gcttccatct gccgccccga atccatcgag cctccgaccc
    11161 aggtttgcca gctgaggagc caaaggagaa gtcctctcgg aaggtagccg agccagagct
    11221 gatgggaacc cctgatggca cctgctaccc tccaccacca gtaccgagac aggcatctcc
    11281 ccagaacctg gggacccctg gcttgctgca cccccagacc tcacccctgc tgcactcatt
    11341 agtgggccaa catatcctgt ccgtccagca gttcaccaag gatcagatgt ctcacctgtt
    11401 caatgtggca cacacactgc gtatgatggt gcagaaggag cggagcctcg acatcctgaa
    11461 ggggaaggtc atggcctcca tgttctatga agtgagcaca cggaccagca gctcctttgc
    11521 agcagccatg gcccggctgg gaggtgctgt gctcagcttc tcggaagcca catcgtccgt
    11581 ccagaagggc gaatccctgg ctgactccgt gcagaccatg agctgctatg ccgacgtcgt
    11641 cgtgctccgg cacccccagc ctggagcagt ggagctggcc gccaagcact gccggaggcc
    11701 agtgatcaat gctggggatg gggtcggaga gcaccccacc caggccctgc tggacatctt
    11761 caccatccgt gaggagctgg gaactgtcaa tggcatgacg atcacgatgg tgggtgacct
    11821 gaagcacgga cgcacagtac attccctggc ctgcctgctc acccagtatc gtgtcagcct
    11881 gcgctacgtg gcacctccca gcctgcgcat gccacccact gtgcgggcct tcgtggcctc
    11941 ccgcggcacc aagcaggagg aattcgagag cattgaggag gcgctgcctg acactgatgt
    12001 gctctacatg actcgaatcc agaaggaacg atttggctct acccaggagt acgaagcttg
    12061 ctttggtcag ttcatcctca ctccccacat catgacccgg gccaagaaga agatggtggt
    12121 gatgcacccg atgccccgtg tcaacgagat aagcgtggaa gtggactcgg atccccgcgc
    12181 agcctacttc cgccaggctg agaacggcat gtacatccgc atggctctgt tagccaccgt
    12241 gctgggccgt ttcgactaca aagacgatga cgacaagtag gaattcctgc aggcctcgag
    12301 ggccggcgcg ccgcggccgc tacgtaaatt ccgc
//
