LOCUS       sequence-370657-       13223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Shin J, Mir H, Khurram MA, Fujihara KM, Dynlacht BD, Cardozo TJ, 
            Possemato R
  TITLE     Allosteric regulation of CAD modulates de novo pyrimidine synthesis 
            during the cell cycle.
  JOURNAL   Nat Metab. 2023 Feb 6. doi: 10.1038/s42255-023-00735-9.
  PUBMED    36747088
REFERENCE   2  (bases 1 to 13223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Metab. 
            2023 Feb 6. doi: 10.1038/s42255-023-00735-9."
COMMENT     Sequence Label: pMXS-IRES-BLAST Halo-flag-CAD
FEATURES             Location/Qualifiers
     source          1..13223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..585
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    35..587
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(179..196)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     406..425
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             599..994
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1165..1634
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1764..1781)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1935..2523)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2015..2034)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2687..3478,3479..3547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3310..3329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3548..3652)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             3682..4275
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4338..4695
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4754..5170
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5100..5122
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5138..5154
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5180..5554
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5492..5508
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             5583..6473
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             13146..13169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgcccccccc ccccntctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
       61 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
      121 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
      181 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
      241 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
      301 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
      361 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
      421 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
      481 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
      541 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
      601 ggttcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
      661 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
      721 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
      781 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
      841 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
      901 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
      961 attgctgccc tctggttatg tgtgggaggg ctaagtcgac gataaaataa aagattttat
     1021 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     1081 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     1141 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     1201 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     1261 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     1321 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     1381 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     1441 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     1501 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     1561 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     1621 gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     1681 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     1741 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1801 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1861 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1921 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1981 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2041 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2101 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2161 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2221 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2281 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2341 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2401 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2461 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2521 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2581 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg
     2641 gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2701 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     2761 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     2821 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     2881 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     2941 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3001 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3061 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3121 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3181 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3241 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3301 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3361 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3421 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3481 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3541 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3601 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3661 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
     3721 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
     3781 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     3841 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     3901 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     3961 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     4021 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     4081 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
     4141 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     4201 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     4261 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
     4321 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
     4381 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
     4441 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
     4501 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
     4561 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
     4621 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     4681 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     4741 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     4801 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     4861 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     4921 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     4981 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     5041 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     5101 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     5161 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     5221 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     5281 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     5341 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     5401 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     5461 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     5521 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     5581 ccatggcaga aatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg
     5641 agcgcatgca ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg
     5701 gtaacccgac ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc
     5761 gctgcattgc tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt
     5821 tcttcgacga ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg
     5881 tcgtcctggt cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc
     5941 cagagcgcgt caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg
     6001 aatggccaga atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca
     6061 agctgatcat cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc
     6121 cgctgactga agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg
     6181 agccactgtg gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg
     6241 cgctggtcga agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct
     6301 ggggcacccc aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc
     6361 ctaactgcaa ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg
     6421 acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc ggcgcggccc
     6481 tagtgttgga ggacgggtcg gtcctgcggg gccagccctt tggggccgcc gtgtcgactg
     6541 ccggggaagt ggtgtttcaa accggcatgg tcggctaccc cgaggccctc actgatccct
     6601 cctacaaggc acagatctta gtgctcacct atcctctgat cggcaactat ggcatccccc
     6661 cagatgaaat ggatgagttc ggtctctgca agtggtttga atcctcgggc atccacgtag
     6721 cagcactggt agtgggagag tgctgtccta ctcccagcca ctggagtgcc acccgcaccc
     6781 tgcatgagtg gctgcagcag catggcatcc ctggcttgca aggagtagac actcgggagc
     6841 tgaccaagaa gttgcgggaa caggggtctc tgctggggaa gctggtccag aatggaacag
     6901 aaccttcaag tctgccattc ttggacccca atgcccgccc cctggtacca gaggtctcca
     6961 ttaagactcc acgggtattc aatacagggg gtgcccctcg gatccttgct ttggactgtg
     7021 gcctcaagta taatcagatc cgatgcctct gccagcgtgg ggctgaggtc actgtggtac
     7081 cctgggacca tgcactagac agccaagagt atgagggtct cttcttaagt aatgggcctg
     7141 gtgaccctgc ctcctatccc agtgtcgtat ccacactgag ccgtgtttta tctgagccta
     7201 atccccgacc tgtctttggg atctgcctgg gacaccagct attggcctta gccattgggg
     7261 ccaagactta caagatgaga tatgggaacc gaggccataa ccagccctgc ttgttggtgg
     7321 gctctgggcg ctgctttctg acatcccaga accatgggtt tgctgtggag acagactcac
     7381 tgccagcaga ctgggctcct ctcttcacca acgccaatga tggttccaat gaatgcattg
     7441 tgcacaacag cttgcctttc ttcagtgtcc agtttcaccc agagcaccaa gctggccctt
     7501 cagatatgga actgcttttc gatatctttc tggaaactgt gaaagaggcc acagctggga
     7561 accctggggg ccagacagtt agagagcggc tgactgagcg cctctgtccc cctgggattc
     7621 ccactcccgg ctctggactt ccaccaccac gaaaggttct gatcctgggc tcagggggcc
     7681 tctccattgg ccaagctgga gaatttgact actcgggctc tcaggcaatt aaggccctga
     7741 aggaggaaaa catccagacg ttgctgatca accccaatat tgccacagtg cagacctccc
     7801 aggggctggc cgacaaggtc tattttcttc ccataacacc tcattatgta acccaggtga
     7861 tacgtaatga acgccccgat ggtgtgttac tgacttttgg gggccagact gctctgaact
     7921 gtggtgtgga gctgaccaag gccggggtgc tggctcggta tggggtccgg gtcctgggca
     7981 caccagtgga gaccattgag ctgaccgagg atcgacgggc ctttgctgcc agaatggcag
     8041 agatcggaga gcatgtggcc ccgagcgagg cagcaaattc tcttgaacag gcccaggcag
     8101 ccgctgaacg gctggggtac cctgtgctag tgcgtgcagc ctttgccctg ggtggcctgg
     8161 gctctggctt tgcctctaac agggaggagc tctctgctct cgtggcccca gcttttgccc
     8221 ataccagcca agtgctagta gacaagtctc tgaagggatg gaaggagatt gagtacgagg
     8281 tggtgagaga cgcctatggc aactgtgtca cggtgtgtaa catggagaac ttggacccac
     8341 tgggcatcca cactggtgag tccatagtgg tggcccctag ccagacactg aatgacaggg
     8401 agtatcagct cctgaggcag acagctatca aggtgaccca gcacctggga attgttgggg
     8461 agtgcaatgt gcagtatgcc ttgaaccctg agtctgagca gtattacatc attgaagtga
     8521 atgccaggct ctctcgcagc tctgccctgg ccagtaaggc cacaggttat ccactggctt
     8581 atgtggcagc caagctagca ttgggcatcc ctttgcctga gctcaggaac tctgtgacag
     8641 ggggtacagc agcctttgaa cccagcgtgg attattgtgt ggtgaagatt cctcgatggg
     8701 accttagcaa gttcctgcga gtcagcacaa agattgggag ctgcatgaag agcgttggtg
     8761 aagtcatggg cattgggcgt tcatttgagg aggccttcca gaaggccctg cgcatggtgg
     8821 atgagaactg tgtgggcttt gatcacacag tgaaaccagt cagcgatatg gagttggaga
     8881 ctccaacaga taagcggatt tttgtggtgg cagctgcttt gtgggctggt tattcagtgg
     8941 accgcctgta tgagctcaca cgcatcgacc gctggttcct gcaccgaatg aagcgtatca
     9001 tcgcacatgc ccagctgcta gaacaacacc gtggacagcc tttgccgcca gacctgctgc
     9061 aacaggccaa gtgtcttggc ttctcagaca aacagattgc ccttgcagtt ctgagcacag
     9121 agctggctgt tcgcaagctg cgtcaggaac tggggatctg tccagcagtg aaacagattg
     9181 acacagttgc agctgagtgg ccagcccaga caaattacct atacctaacg tattggggca
     9241 ccacccatga cctcaccttt cgaacacctc atgtcctagt ccttggctct ggcgtctacc
     9301 gtattggctc tagcgttgaa tttgactggt gtgctgtagg ctgcatccag cagctccgaa
     9361 agatgggata taagaccatc atggtgaact ataacccaga gacagtcagc accgactatg
     9421 acatgtgtga tcgactctac tttgatgaga tctcttttga ggtggtgatg gacatctatg
     9481 agctcgagaa ccctgaaggt gtgatcctat ccatgggtgg acagctgccc aacaacatgg
     9541 ccatggcgtt gcatcggcag cagtgccggg tgctgggcac ctcccctgaa gccattgact
     9601 cggctgagaa ccgtttcaag ttttcccggc tccttgacac cattggtatc agccagcctc
     9661 agtggaggga gctcagtgac ctcgagtctg ctcgccaatt ctgccagacc gtggggtacc
     9721 cctgtgtggt gcgcccctcc tatgtgctga gcggtgctgc tatgaatgtg gcctacacgg
     9781 atggagacct ggagcgcttc ctgagcagcg cagcagccgt ctccaaagag catcccgtgg
     9841 tcatctccaa gttcatccag gaggctaagg agattgacgt ggatgccgtg gcctctgatg
     9901 gtgtggtggc agccatcgcc atctctgagc atgtggagaa tgcaggtgtg cattcaggtg
     9961 atgcgacgct ggtgaccccc ccacaagata tcactgccaa aaccctggag cggatcaaag
    10021 ccattgtgca tgctgtgggc caggagctac aggtcacagg acccttcaat ctgcagctca
    10081 ttgccaagga tgaccagctg aaagttattg aatgcaacgt acgtgtctct cgctccttcc
    10141 ccttcgtttc caagacactg ggtgtggacc tagtagcctt ggccacgcgg gtcatcatgg
    10201 gggaagaagt ggaacctgtg gggctaatga ctggttctgg agttgtggga gtaaaggtgc
    10261 ctcagttctc cttctcccgc ttggcgggtg ctgacgtggt gttgggtgtg gaaatgacca
    10321 gtactgggga ggtggccggc tttggggaga gccgctgtga ggcatacctc aaggccatgc
    10381 taagcactgg ctttaagatc cccaagaaga atatcctgct gaccattggc agctataaga
    10441 acaaaagcga gctgctccca actgtgcggc tactggagag cctgggctac agcctctatg
    10501 ccagtctcgg cacagctgac ttctacactg agcatggcgt caaggtaaca gctgtggact
    10561 ggcactttga ggaggctgtg gatggtgagt gcccaccaca gcggagcatc ctggagcagc
    10621 tagctgagaa aaactttgag ctggtgatta acctgtcaat gcgtggagct gggggccggc
    10681 gtctctcttc ctttgtcacc aagggctacc gcacccgacg cttggccgct gacttctccg
    10741 tgcccctaat catcgatatc aagtgcacca aactctttgt ggaggcccta ggccagatcg
    10801 ggccagcccc tcctttgaag gtgcatgttg actgtatgac ctcccaaaag cttgtgcgac
    10861 tgccgggatt gattgatgtc catgtgcacc tgcgggaacc aggtgggaca cataaggagg
    10921 actttgcttc aggcacagcc gctgccctgg ctgggggtat caccatggtg tgtgccatgc
    10981 ctaatacccg gccccccatc attgacgccc ctgctctggc cctggcccag aagctggcag
    11041 aggctggcgc ccggtgcgac tttgcgctat tccttggggc ctcgtctgaa aatgcaggaa
    11101 ccttgggcac cgtggccggg tctgcagccg ggctgaagct ttacctcaat gagaccttct
    11161 ctgagctgcg gctggacagc gtggtccagt ggatggagca tttcgagaca tggccctccc
    11221 acctccccat tgtggctcac gcggagcagc aaaccgtggc tgctgtcctc atggtggctc
    11281 agctcactca gcgctcagtg cacatatgtc acgtggcacg gaaggaggag atcctgctaa
    11341 ttaaagctgc aaaggcacgg ggcttgccag tgacctgcga ggtggctccc caccacctgt
    11401 tcctaagcca tgatgacctg gagcgcctgg ggcctgggaa gggggaggtc cggcctgagc
    11461 ttggctcccg ccaggatgtg gaagccctgt gggagaacat ggctgtcatc gactgctttg
    11521 cctcagacca tgctccccat accttggagg agaagtgtgg gtccaggccc ccacctgggt
    11581 tcccagggtt agagaccatg ctgccactac tcctgacggc tgtaagcgag ggccggctca
    11641 gcctggacga cctgctgcag cgattgcacc acaatcctcg gcgcatcttt cacctgcctc
    11701 cgcaggagga cacctatgtg gaggtggatc tggagcatga gtggacaatt cccagccaca
    11761 tgcccttctc caaggcccac tggacacctt ttgaagggca gaaagtgaag ggcaccgtcc
    11821 gccgtgtggt cctgcgaggg gaggttgcct atatcgatgg gcaggttctg gtacccccgg
    11881 gctatggaca ggatgtacgg aagtggccac agggggctgt tcctcagctc ccaccctcag
    11941 cccctgccac tagtgagatg accacgacac ctgaaagacc ccgccgtggc atcccagggc
    12001 ttcctgatgg ccgcttccat ctgccgcccc gaatccatcg agcctccgac ccaggtttgc
    12061 cagctgagga gccaaaggag aagtcctctc ggaaggtagc cgagccagag ctgatgggaa
    12121 cccctgatgg cacctgctac cctccaccac cagtaccgag acaggcatct ccccagaacc
    12181 tggggacccc tggcttgctg cacccccaga cctcacccct gctgcactca ttagtgggcc
    12241 aacatatcct gtccgtccag cagttcacca aggatcagat gtctcacctg ttcaatgtgg
    12301 cacacacact gcgtatgatg gtgcagaagg agcggagcct cgacatcctg aaggggaagg
    12361 tcatggcctc catgttctat gaagtgagca cacggaccag cagctccttt gcagcagcca
    12421 tggcccggct gggaggtgct gtgctcagct tctcggaagc cacatcgtcc gtccagaagg
    12481 gcgaatccct ggctgactcc gtgcagacca tgagctgcta tgccgacgtc gtcgtgctcc
    12541 ggcaccccca gcctggagca gtggagctgg ccgccaagca ctgccggagg ccagtgatca
    12601 atgctgggga tggggtcgga gagcacccca cccaggccct gctggacatc ttcaccatcc
    12661 gtgaggagct gggaactgtc aatggcatga cgatcacgat ggtgggtgac ctgaagcacg
    12721 gacgcacagt acattccctg gcctgcctgc tcacccagta tcgtgtcagc ctgcgctacg
    12781 tggcacctcc cagcctgcgc atgccaccca ctgtgcgggc cttcgtggcc tcccgcggca
    12841 ccaagcagga ggaattcgag agcattgagg aggcgctgcc tgacactgat gtgctctaca
    12901 tgactcgaat ccagaaggaa cgatttggct ctacccagga gtacgaagct tgctttggtc
    12961 agttcatcct cactccccac atcatgaccc gggccaagaa gaagatggtg gtgatgcacc
    13021 cgatgccccg tgtcaacgag ataagcgtgg aagtggactc ggatccccgc gcagcctact
    13081 tccgccaggc tgagaacggc atgtacatcc gcatggctct gttagccacc gtgctgggcc
    13141 gtttcgacta caaagacgat gacgacaagt aggaattcct gcaggcctcg agggccggcg
    13201 cgccgcggcc gctacgtaaa ttc
//
