LOCUS       sequence-370667-        7355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway promoter element for the zebrafish irg1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM
  TITLE     Macrophage NFATC2 mediates angiogenic signaling during mycobacterial
            infection.
  JOURNAL   Cell Rep. 2022 Dec 13;41(11):111817. doi: 
            10.1016/j.celrep.2022.111817.
  PUBMED    36516756
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-12-13"; volume: "41(11):111817. doi"; pages: " 
            10.1016/j.celrep.2022.111817"
COMMENT     Sequence Label: p5e irg1
FEATURES             Location/Qualifiers
     source          1..7355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      931..1519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1420..1439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1673..1690
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1849..1876)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1968..2054)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2103..2125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2117..2134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2118..2134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2174..2269
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2271..2289
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(6963..6981)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6982..7105
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(7178..7196)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7201..7217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7201..7217)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7319..7338)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(7330..7355,1..784)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
       61 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
      121 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
      181 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
      241 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
      301 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
      361 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
      421 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
      481 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
      541 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
      601 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
      661 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
      721 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
      781 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
      841 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
      901 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
      961 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1021 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1081 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1141 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1201 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1261 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1321 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1381 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1441 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1501 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1561 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1621 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1681 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     1741 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     1801 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     1861 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     1921 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     1981 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     2041 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     2101 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccg agttaacgct
     2161 accatggagc tccaaataat gattttattt tgactgatag tgacctgttc gttgcaacaa
     2221 attgataagc aatgcttttt tataatgcca actttgtata gaaaagttgg taatacgact
     2281 cactataggg ccggggcagt gaggggtatg gaaaaagttt gtgcgaggtg tgttgtgttc
     2341 atcagcctaa ttgtgggtag aagctttcct tcagttggct gcatgaccag aagtagaaaa
     2401 gttcagaaaa gtctgtggct tgggtgtgcg ttgctgatga ttctcttgtc ttacctcatg
     2461 cactgcctgg tgtagatctc ccagaaggag ggaggctcag ctcctacaac aaggcctgcc
     2521 gtttgcattt atttatttat ttgtttaaca ataattattt ttatggcatt ttactgagta
     2581 atgagcattt gtaacgttac cacaccatga aaagttgaaa ctacctaata tttaagagta
     2641 aaaacttttc ttcagaacca catggtcact atttaaggtc gatataattt cagaatgatt
     2701 aattgacctt gttacactga atgtatgact tctaacaagc tgcatatgtt tcctggatac
     2761 ttcgatcaat cacaacattt ttaaaaagta cctttgcgat tcacaggtgt ttttcttttt
     2821 ttacacttct gaagtgtata ccaggactat gatctgtgct cctacaaatt tgagtggtga
     2881 caatttaatt ctacagatta acaaagcaac atttaggcag cctactgact ttttagtagc
     2941 aatattcaaa atattgtatg ataaaatagt gctcagcata attgagtaca ccccatttag
     3001 aaaatgaata ttttatcctt tttttttgtg aatataggca acgtattttg gtgtatttaa
     3061 acaaaactga tttattaaac agatatattt aataaaataa tatttttgtt caccaaacaa
     3121 ttttgaaatt gaaagataac acaattaaat tcaaacaaaa tattgcaaaa aaatgcaaaa
     3181 tttcaactaa actttcattt tttgcttctc ttgatttttc ctctttttta tgtgtattta
     3241 ttatttttcc ataacatata aatttgcctt tactagtttc tggactgtta gaagttattt
     3301 tgttagataa gctccagatt tggcttcagt actgactaat ctaatgtata tgcacaaata
     3361 taatattgta ctgcttccta ttaaacatct gaatttaaaa gatagatttg tgaggggtgg
     3421 tcttatgctg agcactgtag ataataaaat gtaaaaaaat aataataata attaaaaaac
     3481 tgtaaaatag cgccacacaa aaatactata gcttatacga accattttga aacctaattt
     3541 acataatcag aaatgcacaa tgtgttgttt ctaactacca aaaatgtcaa taaaagttgc
     3601 ttcgttattc ttttcaacat aatatttgtg agtaaagggt caaggcttca taaattgaag
     3661 cataaataca cagaaaacac caatgaatca caaactctgg gaaactcaat gaagttaatt
     3721 caatgaaaca ttttacagtt taagacaaca acacttattt gtacattcca agaaagccaa
     3781 tttatgttta tttaagaata taacataaat gtatgaacat tacactgaat cacaattttt
     3841 actatatgac atatttgtat gttttatttt caaccagcca aatgctgcag cagaaatcca
     3901 gcttcatcag accagaaaaa acttctaatt ttatatgatt tctttgctga gtgtcgttgc
     3961 tgtgctcttg tgcttctgct gctggagctc atctgcttca cagttcactg ttgtgcctcc
     4021 tttctaacaa gaagttattt gagctacttt ctttcagctt gacttttgtc tggtcattct
     4081 tctctgacct ctaaatttca caagacattt tttgccttca gcacttcctc tcacactgga
     4141 tatcatttct ttcacagatt attgtctgta aaccctagaa atcgttctgt gcaggagttt
     4201 cagtttctga attactcaga gcagccaatc tggccacact ggtggttctt ccctattttg
     4261 atgatctgtt ttgaacttca gcagataatc ttggacattc catgatccct aaaagagaac
     4321 tgagctggcg ccttgctatg tgtatattgt ttggatagac aattgtttta accatcattt
     4381 gttaatcagg tgtaactgga gctggatgag tgtatgttca cccaaaaatg acagttttgt
     4441 catcatttac tcattcttga ttcgttccaa actagttgat gtttttattt tttttatttt
     4501 tgttgtgttg ttctgttgaa cacgagaaag tatgttgtag aatgctggaa accgaaagcc
     4561 attgacttcc atagtatttg tttttcctat ggaagtcaat aggtatgttt ccacctaatt
     4621 tgctcatcaa ataagtcatg tgattttgtt taacagatca cgtgataaca aaatataggc
     4681 atgatgcatc agcattaatg gactagccag tggacagacc gcattgtaag acatctgaaa
     4741 tttagttttg gtcattataa aatccctcaa tcaatgtctc tcatcaaagt gattgagtat
     4801 tatacctcct ggtcttgagt gctccacaaa agtggtctca cgcttcctac ggtttccatt
     4861 attctttaga aaatattttg tacttaacag aaaaaaagaa gaaaataaac tcaagtcaag
     4921 agtgagtaaa ttatgacaga attttcattt tcttttggtg aatcacttta attcttaagc
     4981 atcgatcaca cattgtgcac ctgaacacag tcctagagtg tattttacac ccagttcagt
     5041 ttaagagtac atcagtaagc tgaaatgagt tgagttcatc aaaaatatca gtcttaactg
     5101 atatttaaat aatactatta aataatactg tataaataat actatttatt gatacagttt
     5161 tattgtgcag catcaaaata cataatttaa tctttaaaca tttccctttg aacaaacatg
     5221 atatgcaaaa cttaaactat tttaaacttt tgatttttac attaaatgca ttgaatcatc
     5281 tatttctttc agtttacagg agccaaactc atgtgactat gaggaagcaa tgaccaacag
     5341 aacgtactct ttaaactcta atacgcaata gcaaaagaat ttccagattt cacaaatgct
     5401 ggagagatac acttactgca gattcaagag atcaggaaat gtgagctcat attctaatat
     5461 gatatcaact gattatacag tccaaagcat ctaagcagaa tacgtttaat gtaaggacag
     5521 tgaacttctg gagtttacaa cctctacata gatttatgta aaatacatta aaaaaattat
     5581 attacatata gatgtgtaaa aaaacacatc ctgagatcct cagtttctag attcacgatt
     5641 tattgtttaa aaaaaacatc tgttttgttc agaaaactgc tgatgacatt tcttataaat
     5701 ttaaaagtga aaatcttgtc atagcaaatg tcaatttttt tttttaaatg ttttcagaca
     5761 acacaagaac agttcgtaac ttcaaagttg ttaagaaagc aatatttgca gttataatct
     5821 aatttaatca catgaattga aaatatttgt aattctggcc aactctcatt ttatgcaaaa
     5881 tgaaaacact ttatacgaaa ttcagaagca atgtcacact gaaaaacatc ataggattac
     5941 ttagacatga caatgttttt agtaaattgt gacttattaa actgagttag catgttctaa
     6001 taaattagaa aagttacaca aactgtgtta agtcagttta atgtaatgta agttcaaatg
     6061 actcatgagg ttagtttgat tcagcttaaa aatttaaggc aacgagtttt tttttgtttt
     6121 gttttttttt taccgtgcat cagatcttta aaggatgaaa cacatgtgaa agaaattttt
     6181 tgattaatat atacacacac actaccagtc aaaagtttgg ggtcaggttt tttttcttct
     6241 tttttttttt ttaaagaaaa tggttctgtt cattaaggcg gcatttatta aatgtaaaaa
     6301 cattttaaat tgtgaaatat ttattaaaat atttattaaa ataactgctt tcttattgta
     6361 tgtagtttca aataaaatta ttactcccgt catttttgct tctattacta ttactatgaa
     6421 tattagtgat ttctgaagga tcatgtgact ctgaacactg gagtaatgaa gctgaaaatt
     6481 cattttaaaa tcacaggaat aaatgattaa attaaatgat aaactaattc tgaacagtta
     6541 tttcatactg caaaaacatt tcagtttaac agtttgtact gtagttttga tgaaacaaat
     6601 gcagccttgg tgagcaggag aagcttattt taaaacattt taaaatccta ctgaccctaa
     6661 actagtatat acatatacta gtttattcaa aaccatttct tttacatctt actaaatatt
     6721 cagtgtaaag aatattgcaa aatcaaaaca tatgattaaa aatgtccttc caatgtgaac
     6781 tgtgtgagct gcgcatgcgt gataccataa ggtagtcaca aattgtgaaa tactgctctt
     6841 cacgctattg tggtttagta acactggaaa gttggtggtt agtgaggtcg ttctgccagt
     6901 agagatgtta gtatataagg ctgtcaaact ggtcaaatca agattcagag ctcgcacagg
     6961 tccctttagt gagggttaat tcaagtttgt acaaaaaagt tgaacgagaa acgtaaaatg
     7021 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
     7081 aaacacaaca tatgcagtca ctatgaacca actacttaga tggtattagt gacctgtaga
     7141 attcgagctc tagagctgca gggcggccgc gatatcccct atagtgagtc gtattacatg
     7201 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     7261 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
     7321 aggggtgtta tgagccatat tcaacgggaa acgtc
//
