LOCUS       sequence-370681-       12656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12656)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 12656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS538
FEATURES             Location/Qualifiers
     source          1..12656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             680..700
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(10781..10880)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10897..10916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10898..10916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10921..10937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10921..10937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11039..11058)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             11050..11859
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11117..11136)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      12006..12594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12495..12514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcg atgactgctc caaagaagaa gcgtaaggta ccggtagaaa aaatggacaa
      721 gaagtactcc atcggactcg acatcggaac caactccgtc ggatgggccg tcatcaccga
      781 cgagtacaag gtcccatcca agaagttcaa ggtcctcgga aacaccgacc gtcactccat
      841 caagaagaac ctcatcggag ccctcctctt cgactccgga gagaccgccg aggccacccg
      901 tctcaagcgt accgcccgtc gtcgttacac ccgtcgtaag aaccgtatct gctacctcca
      961 ggagatcttc tccaacgaga tggccaaggt cgacgactcc ttcttccacc gtctcgagga
     1021 gtccttcctc gtcgaggagg acaagaagca cgagcgtcac ccaatcttcg gaaacatcgt
     1081 cgacgaggtc gcctaccacg agaagtaccc aaccatctac cacctccgta agaagctcgt
     1141 cgactccacc gacaaggccg acctccgtct catctacctc gccctcgccc acatgatcaa
     1201 gttccgtgga cacttcctca tcgagggaga cctcaaccca gacaactccg acgtcgacaa
     1261 gctcttcatc cagctcgtcc agacctacaa ccagctcttc gaggagaacc caatcaacgc
     1321 ctccggagtc gacgccaagg ccatcctctc cgcccgtctc tccaagtccc gtcgtctcga
     1381 gaacctcatc gcccagctcc caggagagaa gaagaacgga ctcttcggaa acctcatcgc
     1441 cctctccctc ggactcaccc caaacttcaa gtccaacttc gacctcgccg aggacgccaa
     1501 gctccagctc tccaaggaca cctacgacga cgacctcgac aacctcctcg cccagatcgg
     1561 agaccagtac gccgacctct tcctcgccgc caagaacctc tccgacgcca tcctcctctc
     1621 cgacatcctc cgtgtaagga gttgaacggc tgaaaaatcg atattttgag cgaaaaaagc
     1681 cggaaaaatg gattttatcg gataaaattt gattttttga gctgagaaat gcctttatag
     1741 acgaatttcc ttgtcaaatt gttaaaaact gcaaaatttg ccaaaaaaaa agcatttttt
     1801 atattttttt ttacaaaaaa ttccacaaaa atggcaaaat ttgactaaaa atgccaattt
     1861 tgtcgttttc tccgtgccac agcggccgaa aatcgatttt taagcgattt tcgggtgaaa
     1921 aagtgtaaaa accgactgaa aatccagctg aaaacgacaa aaacggtaag ttttagctaa
     1981 ttttcacctt tttttcgata tttttttact aaaaaaaaac cgaaaaattg agtttttttc
     2041 aaatttctgc aaaaattctg caattttagc aatttccata ctattttgac gccgaaaaag
     2101 cttaaaaata tgatttttag aggtttttaa tggaaaattc atggattttt caaatttttc
     2161 cacgaaaatc attgaaaaat ttgaaaaaat tgatttccta ttcaaagttc tagctaaaaa
     2221 ctgcaatttt agcaatttcc atactatttt agtactgaaa aagcctaaaa atgtgatttt
     2281 cagaggattt caatggaaaa ttcatggatt tttccaattt ttcaatgaaa accattgaaa
     2341 aaccctggaa aattgagttt ttcttcaaag tttaggaaat ttatgagaat ttttgaataa
     2401 aaaattttta aatttgaaaa aaattgaaat tttaattcta aattttaaag aaagttgatt
     2461 ttttaataat tttttttttg aataaaaaaa aatcaacttt ctttaaaatt tagaattaaa
     2521 atttcaattt ttttattcaa aaattctcat aaatttcgta aacttttaat aaaaactcaa
     2581 tttttccgat ttttttcgat gattttcact aaaaaattgg aaaaattcat caatttccta
     2641 ttgaaaacct ctaaaaatta tatttttttt gcttttccga cgtcaaaata gtatggaaaa
     2701 tgctaaaatt gcagaatttt tccagaaatt tgaagaaaaa actcaatttt ttccgttttt
     2761 tcaatgattt tcatgaaaaa attggaaaat tttgtaaatt ttccattgaa aacctctgaa
     2821 aatttggaaa ttttcgaatt taaatgttta aaaaaaattg aatattttgc gagtttttga
     2881 attttttttg agaatttttg aatttttttc acccaaaatt ttttcaattt ttcaggtcaa
     2941 caccgagatc accaaggccc cactctccgc ctccatgatc aagcgttacg acgagcacca
     3001 ccaggacctc accctcctca aggccctcgt ccgtcagcag ctcccagaga agtacaagga
     3061 gatcttcttc gaccagtcca agaacggata cgccggatac atcgacggag gagcctccca
     3121 ggaggagttc tacaagttca tcaagccaat cctcgagaag atggacggaa ccgaggagct
     3181 cctcgtcaag ctcaaccgtg aggacctcct ccgtaagcag cgtaccttcg acaacggatc
     3241 catcccacac cagatccacc tcggagagct ccacgccatc ctccgtcgtc aggaggactt
     3301 ctacccattc ctcaaggaca accgtgagaa gatcgagaag atcctcacct tccgtatccc
     3361 atactacgtc ggaccactcg cccgtggaaa ctcccgtttc gcctggatga cccgtaagtc
     3421 cgaggagacc atcaccccat ggaacttcga ggaggtcgtc gacaagggag cctccgccca
     3481 gtccttcatc gagcgtatga ccaacttcga caagaacctc ccaaacgaga aggtcctccc
     3541 aaagcactcc ctcctctacg agtacttcac cgtctacaac gagctcacca aggtcaagta
     3601 cgtcaccgag ggaatgcgta agccagcctt cctctccgga gagcagaaga aggccatcgt
     3661 cgacctcctc ttcaagacca accgtaaggt caccgtcaag cagctcaagg aggactactt
     3721 caagaagatc gagtgcttcg actccgtcga gatctccgga gtcgaggacc gtttcaacgc
     3781 ctccctcgga acctaccacg acctcctcaa gatcatcaag gacaaggact tcctcgacaa
     3841 cgaggagaac gaggacatcc tcgaggacat cgtcctcacc ctcaccctct tcgaggaccg
     3901 tgagatgatc gaggtgctga agctcgaaaa tttggagctt ttacacgaga tttttgaaga
     3961 aaaagcctga aaatcgagag attttgagct gtggaattcg ttgaaattac ataattcgcg
     4021 cccgtaattc tttaaaaaaa cgctgaaatt tgcagatttt gccagaaagt tgaagaaaaa
     4081 tctacttttc cggcattctc tattattttc atggaaaatt agaaaaatcc atgaataaac
     4141 agtgaaaatt gctgatttta cctgaaattt tgaaaaaaaa aaaaatctaa aatcaaggtt
     4201 ttaagcctaa aaactgcaga tttcagccaa aattgtgaaa aaagctgatt ttttgctatt
     4261 ttgagctttt ccatggggtg gaaattgcca aaaattacgg aacattcaca caaatttgct
     4321 gaataatcca attttccagt gttttctaga gtttccgtag aaattttgaa aaaacccagt
     4381 aaattttcaa tcggaaaacc tctaaaaaca tcgtttcagg cctataaata gtaaaaatta
     4441 ccgattttat tcgaaaattt aaggaaaagt cgtgaaaaac gagattttaa gcctcaaaac
     4501 tgcagattct atctaaaatt aaaaaaaaaa aacataaata tccgaatttt tggaggaaaa
     4561 gcctgaaaat cgatatttta agcctcaaaa ctgaagattt cagccaaaaa aaaaaacaga
     4621 tttttcgcta ttttatgtgc caaaaaacca cattccgagc ttttccagag tggaaattgc
     4681 caaaaattac agaattttcc cagaaattag caaaaaaaaa acccattttt caggcgtttt
     4741 caacgatttt catagcaaat ttgaaaaatc catgaatttt ttaattatag aaaaccctct
     4801 gaaaattgca gttttaagct attccagcat tcaaatagta tggaaattgc taaaaattga
     4861 agaattttcc caaaaatttg atgaaaactt atttttctgg cgttttcgat gattttcatg
     4921 gaatttcgag aaaattaatg aattttcaat taaaaaacgt ctgcaaaatc acatttttta
     4981 aagctttttc agcaacaaaa tagtatggaa attgcttttt aaaaaagcgg aatttttgga
     5041 aaaaaggtta aattttcagt cagtttttgc actttttgac tgaaaattca ctgaaattag
     5101 ggaaaaattc cggaaaaatg attttttcga attgtagggc aaattttgca atttcaagca
     5161 atttccataa tattttaatg ctggaaaagc tcaaatttac gttttaatgg gattttagtg
     5221 aaaaattcat ggattttccg aaaaatttgc aggaaaatca tagtaaagac ctcaaaaaat
     5281 caataaaaaa tttaaaaaat tggacaatca catttctgcg taaaatctgc actttttcag
     5341 taaaaaatac cgattttccg tgaaaattct cgatttttaa tcgaaaatct cgaaattttt
     5401 acaaatttca gcaccaaaat cgttgaaaat tctgaaaatt tgaatttttt ttccttgttt
     5461 tttgagtaaa atttgcactt tttcagtaaa gaataccgat tttccgtgaa aattctcggt
     5521 ttttaatcga aaatctcgaa atttttacaa atttcagctc cgaaatagct gaaaattgga
     5581 caaaaatttc aatttttgcc ctgttttttg agtaaaatct gcacttttta gttgaaaaaa
     5641 aacacggatt ttgtaggaaa atttgggaaa aatctccaaa ttttcacaat tttccagtaa
     5701 aaacagcaat ttttaaaacg gtttttaatc aaaaatcttt agaattttcc aaatttcagc
     5761 accaaaaaag ctaaaaatcg ctgaaatttt cgacaaattc caaatttcca ggagcgtctc
     5821 aagacctacg cccacctctt cgacgacaag gtcatgaagc agctcaagcg tcgtcgttac
     5881 accggatggg gacgtctctc ccgtaagctc atcaacggaa tccgtgacaa gcagtccgga
     5941 aagaccatcc tcgacttcct caagtccgac ggattcgcca accgtaacgt aagtttaaac
     6001 atgattttac taactaacta atctgattta aattttcagt tcatgcagct catccacgac
     6061 gactccctca ccttcaagga ggacatccag aaggcccagg tctccggaca gggagactcc
     6121 ctccacgagc acatcgccaa cctcgccgga tccccagcca tcaagaaggg aatcctccag
     6181 accgtcaagg tcgtcgacga gctcgtcaag gtcatgggac gtcacaagcc agagaacatc
     6241 gtcatcgaga tggcccgtga gaaccagacc acccagaagg gacagaagaa ctcccgtgag
     6301 cgtatgaagc gtatcgagga gggaatcaag gagctcggat cccagatcct caaggagcac
     6361 ccagtcgaga acacccagct ccagaacgag aagctctacc tctactacct ccagaacgga
     6421 cgtgacatgt acgtcgacca ggagctcgac atcaaccgtc tctccgacta cgacgtcgac
     6481 cacatcgtcc cacagtcctt cctcaaggac gactccatcg acaacaaggt cctcacccgt
     6541 tccgacaaga accgtggaaa gtccgacaac gtcccatccg aggaggtcgt caagaagatg
     6601 aagaactact ggcgtcagct cctcaacgcc aagctcatca cccagcgtaa gttcgacaac
     6661 ctcaccaagg ccgagcgtgg aggactctcc gagctcgaca aggccggatt catcaagcgt
     6721 cagctcgtcg agacccgtca gatcaccaag cacgtcgccc agatcctcga ctcccgtatg
     6781 aacaccaagt acgacgagaa cgacaagctc atccgtggtt ggtttttccg agagaaaaac
     6841 gctgaaaaat gccgaaaatt ttgaatttgc aggcttttta ggtgcaaaag tacggaaaaa
     6901 tcggaaaaaa atcaggaaaa catggggaaa atccaatttt ccagaaaaaa tgtttgaaaa
     6961 aattgaaaac ttttgttttt ttagtaataa agttttaaaa aatggcaaaa atagctgaaa
     7021 acagcgaaaa aaaaaaatgt ttttttcagg aaaaaatttg caaaaaaatg aagagcaaca
     7081 ataattccct tttttttctg tgtttttttc ggaaaaattg agatcgaaaa cgaaaaacag
     7141 cgaaaattcc caattttccc agtttttaaa acattttttt gtaaaaattt ggaaaaaacg
     7201 agttttttca gaaaaaaatg tgaaaaaaaa ggtaaattac caatttatgc ttttttttcg
     7261 atcgagtaaa aaatcgattt ttccaagatt tttcgcgaaa aattcaaaaa aaaagaacga
     7321 aaattctcga ttttctactt tttttcgaaa aaaaaaggaa attatagatt tttccaagat
     7381 ttttcgcgaa aaaattcgaa aaaaatgttt ttttcgaaaa tttcagattt tctaattttt
     7441 tcagaagaat tcgaaaaaaa aaatcaaaaa attcgcaatt tttagatttt tttaaaatct
     7501 caattttcta taattatttt cctaaaaaaa tgtaattaaa aaaccgctaa aaacggtaaa
     7561 ttccaaattt ttgcattttt tcaaaaaaaa tttgaaaaaa aaaatcagaa aattctcaaa
     7621 tctattccct ttttttaaaa tttttcccaa accaaaaaaa aaaaacggta aattttcaaa
     7681 tttagcattt ttccaaaatt aaacataaaa aaaaacgcaa atttccaaat ttcgtttttt
     7741 tttcaaaaaa gttgaaaaat ccagaaaata ccctcttgtc catgaaaaaa tacgaaaatt
     7801 ttcaataaaa aatcaatttc ttcttaaaaa attaattctc gatttttcca tgaaaaaaaa
     7861 tcaaatatta attaaatttg aaaaaaaaaa gaatttatct aatagtttcg ttttttttca
     7921 gaggaaaaat ttgaaaaaaa aaacagtaaa ttctctttct ttttgaaaaa aaaaattatc
     7981 gattcttcca agttttttcc cgaaaaaatc gacaaaaaaa cgagaatttc cgattttcta
     8041 tttttttttt caaaaaaatg tgaaaaaaaa aattaaaaaa ttccaatttt gcattttttt
     8101 ttcaaaaaaa atttaaaaac accgtataat tcccgatttt tccatgaaaa aatacgaaaa
     8161 attccaataa aaatcaacta ttttaaaaat ttgccgtttt ttcagaggtc aaggtcatca
     8221 ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccagttctac aaggtccgtg
     8281 agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc ggaaccgccc
     8341 tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac aaggtctacg
     8401 acgtccgtaa gatgatcgcc aagtccgagc aggagatcgg aaaggccacc gccaagtact
     8461 tcttctactc caacatcatg aacttcttca agaccgagat caccctcgcc aacggagaga
     8521 tccgtaagcg tccactcatc gagaccaacg gagagaccgg agagatcgtc tgggacaagg
     8581 gacgtgactt cgccaccgtc cgtaaggtcc tctccatgcc acaggtcaac atcgtcaaga
     8641 agaccgaggt ccagaccgga ggattctcca aggagtccat cctcccaaag cgtaactccg
     8701 acaagctcat cgcccgtaag aaggactggg acccaaagaa gtacggagga ttcgactccc
     8761 caaccgtcgc ctactccgtc ctcgtcgtcg ccaaggtcga gaagggaaag tccaagaagc
     8821 tcaagtccgt caaggagctc ctcggaatca ccatcatgga gcgttcctcc ttcgagaaga
     8881 acccaatcga cttcctcgag gccaagggat acaaggaggt caagaaggtg cttacaattt
     8941 tctggagaaa aatattttaa aaaataacga aaatggaccg aatttttgga gaaaatttta
     9001 aaaaaaacga catttttccc aaaaaattga aaaatttccg aaaaaacgtt caaaattttc
     9061 aaaaaaggag catttttcga aaaaaaaaac gataatacgg aaattttttg attttccagt
     9121 ttttttctca aagaaaatcg gaaaaaggtt ttttttcttt aaaaacgtcc aaaatttttc
     9181 gaaaaaagag aaaattttcg attttccgat ttttttcaga aaaaaattga aaaaaaaaga
     9241 aattttccga ttttctcaga gaaagacgaa aagacgatat ttttcggaaa aaagttgaaa
     9301 aatcaaaaaa aaaaccgaaa aacggccgaa attttctaaa aattggaaat attgtcgatt
     9361 tttcgaaaaa aaagagtcaa aagttttcaa aaaaatatat tttttcgatt ttccgttttt
     9421 tttttcagta aacattgaaa caaacgaaat ttttccaaaa aaatccaatt attttcccaa
     9481 atatcgaaat atttttgatt ttccgttttt ttcagaaaaa agttaaaaaa aaaagggaaa
     9541 attaagattt tcctgatttt ttttgagaaa aaatcgataa aaattgaata ttctggaaaa
     9601 aacctttaaa aaattcgaat taaatcgaaa aacgtccaaa attttctaaa aaattgaaat
     9661 tttttttgaa aaaaaacgga aaaatttaga tttcccgact tttttcaatg aaaaatcgaa
     9721 ttttcgttga cttttcatta aaaaatccaa acgcattttt ttccactaaa atctcgaaaa
     9781 agtgtcatat tgcaccatat tgtttccaca ttcatattat tttgagctaa atcaggtgat
     9841 tttctgtatt ttgatactaa tttctatttt tagaaaaaat gtgggaacaa tatggtgcaa
     9901 tatgacattt tttcgagatt ttagtggaga aaaaatgcgt ttggaaacga attttataat
     9961 aaaaagtcaa cgaaaattcg atttttcatg gaaaaatagg gaaatcgaaa acaaatttgc
    10021 ttttttttca aaaaaaaaaa cggaaattcg aaatttttcc gttctttcaa aaaaaaaatc
    10081 gatttttttg gaaatttttt ttttcaaatt tttaaaaatt tccaggacct catcatcaag
    10141 ctcccaaagt actccctctt cgagctcgag aacggacgta agcgtatgct cgcctccgcc
    10201 ggagagctcc agaagggaaa cgagctcgcc ctcccatcca agtacgtcaa cttcctctac
    10261 ctcgcctccc actacgagaa gctcaaggga tccccagagg acaacgagca gaagcagctc
    10321 ttcgtcgagc agcacaagca ctacctcgac gagatcatcg agcagatctc cgagttctcc
    10381 aagcgtgtca tcctcgccga cgccaacctc gacaaggtcc tctccgccta caacaagcac
    10441 cgtgacaagc caatccgtga gcaggccgag aacatcatcc acctcttcac cctcaccaac
    10501 ctcggagccc cagccgcctt caagtacttc gacaccacca tcgaccgtaa gcgttacacc
    10561 tccaccaagg aggtcctcga cgccaccctc atccaccagt ccatcaccgg actctacgag
    10621 acccgtatcg acctctccca gctcggagga gactctggag gatctggagg aactggagga
    10681 tcttccgccg gcgggagctc tatgagccgt agacgaaaag cgaatccgac aaaactgagt
    10741 gaaaacgcga agaagcttgc caaggaagtt gaaaattaac acccagcttt cttgtacaaa
    10801 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
    10861 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
    10921 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
    10981 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
    11041 aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg
    11101 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
    11161 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
    11221 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg
    11281 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
    11341 gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
    11401 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
    11461 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
    11521 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
    11581 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc
    11641 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
    11701 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
    11761 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
    11821 aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg
    11881 taacactggc agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc
    11941 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    12001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    12061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    12121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    12181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    12241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    12301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    12361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    12421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    12481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    12541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    12601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtt
//
