LOCUS       sequence-37069-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Grunwald D, Singer RH
  TITLE     In vivo imaging of labelled endogenous I
  JOURNAL   Nature. 2010 Sep 30. 467(7315):604-7.
  PUBMED    20844488
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Sep 30. 467(7315):604-7."
COMMENT     Sequence Label: UbC NLS-HA-MCP-YFP
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(403..414)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(415..441)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(415..441)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(445..465)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(480..878)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ctcctcgccc ttgctcacca ttctagaatc cgcgtagatg ccggagtttg ctgcgattgc
       61 tgagggaatc gggtttccat cttttaggag accttgcatt gccttaacaa taagctcgca
      121 gtcggaattc gtggcgaaaa ttggaatggt tagttccata tttaagtacg aacgccaggc
      181 gcctttaggc acctcgactt tgatggtgta tttgcgattc tgcgcagagc tctgacgaac
      241 gctacaggtt actttgtaag cctgtgaacg cgagttagag ctgatccatt cagcgatccc
      301 gttagcgaag ttgcttgggg cgacagtcac gtcgccagtt ccgccattgt cgacgagaac
      361 gaactgagta aagttagaag ccattttaac ggctagcata tggcggcctt cgatggcgta
      421 gtcgggcacg tcgtaggggt agccaacttt tcttttcttt tttgggccca tggcggccgc
      481 caagtgacga tcacagcgat ccacaaacaa gaaccgcgac ccaaatcccg gctgcgacgg
      541 aactagctgt gccacacccg gcgcgtcctt atataatcat cggcgttcac cgccccacgg
      601 agatccctcc gcagaatcgc cgagaaggga ctacttttcc tcgcctgttc cgctctctgg
      661 aaagaaaacc agtgccctag agtcacccaa gtcccgtcct aaaatgtcct tctgctgata
      721 ctggggttct aaggccgagt cttatgagca gcgggccgct gtcctgagcg tccgggcgga
      781 aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg tgaggagggg
      841 ggcgcccgcg ggaggcgcca aaacccggcg cggaggccac tagtccaaac tggatctctg
      901 ctgtccctgt aaacacccc
//
