LOCUS       sequence-370708-        9291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human EPHB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 9291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_EPHB4
FEATURES             Location/Qualifiers
     source          1..9291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1842..1861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2033..2412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2413..2616
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2567..2587
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2618..2636
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2639..2657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2667..2691
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2834..2858
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5826..5850)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5897..5920
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5957..5980
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6011..6037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6011..6037
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6038..6385,6386..6388,6389..6997)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7041..7058)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7047..7271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7404..7423)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7555..7602
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7610..8632
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             7610..8632
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8762..8883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8799..8818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8853..8872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8932..8948)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8932..8948)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8945..8967)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8956..8972
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8980..8986,8987..9004,9005..9010))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9025..9046
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9163..9180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     1801 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
     1861 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
     1921 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
     1981 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
     2041 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2101 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2161 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2221 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2281 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2341 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2401 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2461 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2521 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2581 aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc
     2641 cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga
     2701 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctccggact
     2761 ctagcgttta aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
     2821 tctgcagata tcaacaagtt tgtacaaaaa agcaggctcc accatggagc tccgggtgct
     2881 gctctgctgg gcttcgttgg ccgcagcttt ggaagagacc ctgctgaaca caaaattgga
     2941 aactgctgat ctgaagtggg tgacattccc tcaggtggac gggcagtggg aggaactgag
     3001 cggcctggat gaggaacagc acagcgtgcg cacctacgaa gtgtgtgacg tgcagcgtgc
     3061 cccgggccag gcccactggc ttcgcacagg ttgggtccca cggcggggcg ccgtccacgt
     3121 gtacgccacg ctgcgcttca ccatgctcga gtgcctgtcc ctgcctcggg ctgggcgctc
     3181 ctgcaaggag accttcaccg tcttctacta tgagagcgat gcggacacgg ccacggccct
     3241 cacgccagcc tggatggaga acccctacat caaggtggac acggtggccg cggagcatct
     3301 cacccggaag cgccctgggg ccgaggccac cgggaaggtg aatgtcaaga cgctgcgtct
     3361 gggaccgctc agcaaggctg gcttctacct ggccttccag gaccagggtg cctgcatggc
     3421 cctgctatcc ctgcacctct tctacaaaaa gtgcgcccag ctgactgtga acctgactcg
     3481 attcccggag actgtgcctc gggagctggt tgtgcccgtg gccggtagct gcgtggtgga
     3541 tgccgtcccc gcccctggcc ccagccccag cctctactgc cgtgaggatg gccagtgggc
     3601 cgaacagccg gtcacgggct gcagctgtgc tccggggttc gaggcagctg aggggaacac
     3661 caagtgccga gcctgtgccc agggcacctt caagcccctg tcaggagaag ggtcctgcca
     3721 gccatgccca gccaatagcc actctaacac cattggatca gccgtctgcc agtgccgcgt
     3781 cgggtacttc cgggcacgca cagacccccg gggtgcaccc tgcaccaccc ctccttcggc
     3841 tccgcggagc gtggtttccc gcctgaacgg ctcctccctg cacctggaat ggagtgcccc
     3901 cctggagtct ggtggccgag aggacctcac ctacgccctc cgctgccggg agtgccgacc
     3961 cggaggctcc tgtgcgccct gcgggggaga cctgactttt gaccccggcc cccgggacct
     4021 ggtggagccc tgggtggtgg ttcgagggct acgtcctgac ttcacctata cctttgaggt
     4081 cactgcattg aacggggtat cctccttagc cacggggccc gtcccatttg agcctgtcaa
     4141 tgtcaccact gaccgagagg tacctcctgc agtgtctgac atccgggtga cgcggtcctc
     4201 acccagcagc ttgagcctgg cctgggctgt tccccgggca cccagtgggg ctgtgctgga
     4261 ctacgaggtc aaataccatg agaagggcgc cgagggtccc agcagcgtgc ggttcctgaa
     4321 gacgtcagaa aaccgggcag agctgcgggg gctgaagcgg ggagccagct acctggtgca
     4381 ggtacgggcg cgctctgagg ccggctacgg gcccttcggc caggaacatc acagccagac
     4441 ccaactggat gagagcgagg gctggcggga gcagctggcc ctgattgcgg gcacggcagt
     4501 cgtgggtgtg gtcctggtcc tggtggtcat tgtggtcgca gttctctgcc tcaggaagca
     4561 gagcaatggg agagaagcag aatattcgga caaacacgga cagtatctca tcggacatgg
     4621 tactaaggtc tacatcgacc ccttcactta tgaagaccct aatgaggctg tgagggaatt
     4681 tgcaaaagag atcgatgtct cctacgtcaa gattgaagag gtgattggtg caggtgagtt
     4741 tggcgaggtg tgtcgggggc ggctcaaggc cccagggaag aaggagagct gtgtggcaat
     4801 caagaccctg aagggtggct acacggagcg gcagcggcgt gagtttctga gcgaggcctc
     4861 catcatgggc cagttcgagc accccaatat catccgcctg gagggcgtgg tcaccaacag
     4921 catgcccgtc atgattctca cagagttcat ggagaacggc gccctggact ccttcctgcg
     4981 gctaaacgac ggacagttca cagtcatcca gctcgtgggc atgctgcggg gcatcgcctc
     5041 gggcatgcgg taccttgccg agatgagcta cgtccaccga gacctggctg ctcgcaacat
     5101 cctagtcaac agcaacctcg tctgcaaagt gtctgacttt ggcctttccc gattcctgga
     5161 ggagaactct tccgatccca cctacacgag ctccctggga ggaaagattc ccatccgatg
     5221 gactgccccg gaggccattg ccttccggaa gttcacttcc gccagtgatg cctggagtta
     5281 cgggattgtt atgtgggagg tgatgtcatt tggggagagg ccgtactggg acatgagcaa
     5341 tcaggacgtg atcaatgcca ttgaacagga ctaccggctg cccccgcccc cagactgtcc
     5401 cacctccctc caccagctca tgctggactg ttggcagaaa gaccggaatg cccggccccg
     5461 cttcccccag gtggtcagcg ccctggacaa gatgatccgg aaccccgcca gcctcaaaat
     5521 cgtggcccgg gagaatggcg gggcctcaca ccctctcctg gaccagcggc agcctcacta
     5581 ctcagctttt ggctctgtgg gcgagtggct tcgggccatc aaaatgggaa gatacgaaga
     5641 aagtttcgca gccgctggct ttggctcctt cgagctggtc agccagatct ctgctgagga
     5701 cctgctccga atcggagtca ctctggcggg acaccagaag aaaatcttgg ccagtgtcca
     5761 gcacatgaag tcccaggcca agccgggaac cccgggtggg acaggaggac cggccccgca
     5821 gtacgaccca gctttcttgt acaaagtggt tgatatcaag gccgccgaca tcaccagcct
     5881 gtacaagaaa gtggggtgga gccatcccca gttcgagaag ggcggaggat ctggcggcgg
     5941 aagcggcgga ggaagctggt cccaccctca gtttgagaaa ggcaccgagc tgggcagcac
     6001 catggccagc tacccctacg acgtgcccga ctacgccaag gacaacaccg tgcccctgaa
     6061 gctgatcgcc ctgctggcca acggcgagtt ccactctggc gagcagctgg gcgagacact
     6121 gggcatgagc agagccgcca tcaacaagca catccagacc ctgcgggact ggggcgtgga
     6181 cgtgttcaca gtgcccggca agggctacag cctgcccgag cccatccagc tgctgaacgc
     6241 caagcagatc ctgggccagc tggatggcgg atctgtggcc gtgctgcccg tgatcgacag
     6301 caccaaccag tatctgctcg accggatcgg cgagctgaag tccggcgacg cctgtatcgc
     6361 cgagtaccag caggccggca gaggcggcag gggcagaaag tggttcagcc ccttcggcgc
     6421 caacctgtac ctgagcatgt tctggagact ggaacagggc ccagccgccg ctattggcct
     6481 gagcctggtc atcggcatcg tgatggccga ggtgctgcgg aagctgggcg ccgacaaagt
     6541 gcgcgtgaag tggcccaacg acctgtatct gcaggaccgg aagctggccg gcatcctggt
     6601 ggaactgacc ggcaaaacag gcgacgccgc ccagatcgtg atcggcgctg gcatcaacat
     6661 ggccatgcgg cgggtggaag agagcgtggt caaccagggc tggatcaccc tgcaggaagc
     6721 cggcatcaac ctggaccgga acaccctggc cgccatgctg atcagagagc tgagagccgc
     6781 cctggaactg ttcgagcagg aaggcctggc cccctacctg agcagatggg agaagctgga
     6841 caacttcatc aaccggcccg tgaaactgat catcggcgac aaagagatct tcggcatcag
     6901 ccggggcatc gacaagcagg gggccctgct cctggaacag gacggcatca tcaagccctg
     6961 gatgggcggc gagatcagcc tgcggagcgc cgagaagtga tgactcgagt ctagagggcc
     7021 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     7081 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7141 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     7201 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7261 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     7321 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7381 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7441 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7501 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta cctagaagtt
     7561 cctattccga agttcctatt ctctagaaag tataggaact tccttggcca aaaagcctga
     7621 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     7681 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     7741 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     7801 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     7861 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     7921 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     7981 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     8041 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     8101 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     8161 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     8221 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     8281 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     8341 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     8401 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     8461 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     8521 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     8581 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtact
     8641 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8701 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8761 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8881 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     8941 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9001 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9061 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9121 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc a
//
