LOCUS       sequence-370709-       10440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human INSR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_INSR
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    112..136
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4253..4277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4324..4347
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4384..4407
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4438..4464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             4438..4464
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4465..4812,4813..4815,4816..5424)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(5468..5485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5474..5698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5831..5850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5982..6029
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6037..7059
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6037..7059
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7189..7310
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7226..7245)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7280..7299
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7359..7375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7359..7375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7372..7394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7383..7399
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7407..7413,7414..7431,7432..7437))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7452..7473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7590..7607)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7761..8349)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7841..7860)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8520..9311,9312..9380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9143..9162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9381..9485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9560..9579)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9751..10130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10131..10334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10285..10305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10336..10354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10357..10375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10385..10409
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccatagaag acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagcttg
       61 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc aacaagtttg
      121 tacaaaaaag caggctccac catgggcacc gggggccggc ggggggcggc ggccgcgccg
      181 ctgctggtgg cggtggccgc gctgctactg ggcgccgcgg gccacctgta ccccggagag
      241 gtgtgtcccg gcatggatat ccggaacaac ctcactaggt tgcatgagct ggagaattgc
      301 tctgtcatcg aaggacactt gcagatactc ttgatgttca aaacgaggcc cgaagatttc
      361 cgagacctca gtttccccaa actcatcatg atcactgatt acttgctgct cttccgggtc
      421 tatgggctcg agagcctgaa ggacctgttc cccaacctca cggtcatccg gggatcacga
      481 ctgttcttta actacgcgct ggtcatcttc gagatggttc acctcaagga actcggcctc
      541 tacaacctga tgaacatcac ccggggttct gtccgcatcg agaagaacaa tgagctctgt
      601 tacttggcca ctatcgactg gtcccgtatc ctggattccg tggaggataa ttacatcgtg
      661 ttgaacaaag atgacaacga ggagtgtgga gacatctgtc cgggtaccgc gaagggcaag
      721 accaactgcc ccgccaccgt catcaacggg cagtttgtcg aacgatgttg gactcatagt
      781 cactgccaga aagtttgccc gaccatctgt aagtcacacg gctgcaccgc cgaaggcctc
      841 tgttgccaca gcgagtgcct gggcaactgt tctcagcccg acgaccccac caagtgcgtg
      901 gcctgccgca acttctacct ggacggcagg tgtgtggaga cctgcccgcc cccgtactac
      961 cacttccagg actggcgctg tgtgaacttc agcttctgcc aggacctgca ccacaaatgc
     1021 aagaactcgc ggaggcaggg ctgccaccag tacgtcattc acaacaacaa gtgcatccct
     1081 gagtgtccct ccgggtacac gatgaattcc agcaacttgc tgtgcacccc atgcctgggt
     1141 ccctgtccca aggtgtgcca cctcctagaa ggcgagaaga ccatcgactc ggtgacgtct
     1201 gcccaggagc tccgaggatg caccgtcatc aacgggagtc tgatcatcaa cattcgagga
     1261 ggcaacaatc tggcagctga gctagaagcc aacctcggcc tcattgaaga aatttcaggg
     1321 tatctaaaaa tccgccgatc ctacgctctg gtgtcacttt ccttcttccg gaagttacgt
     1381 ctgattcgag gagagacctt ggaaattggg aactactcct tctatgcctt ggacaaccag
     1441 aacctaaggc agctctggga ctggagcaaa cacaacctca ccatcactca ggggaaactc
     1501 ttcttccact ataaccccaa actctgcttg tcagaaatcc acaagatgga agaagtttca
     1561 ggaaccaagg ggcgccagga gagaaacgac attgccctga agaccaatgg ggaccaggca
     1621 tcctgtgaaa atgagttact taaattttct tacattcgga catcttttga caagatcttg
     1681 ctgagatggg agccgtactg gccccccgac ttccgagacc tcttggggtt catgctgttc
     1741 tacaaagagg ccccttatca gaatgtgacg gagttcgacg ggcaggatgc gtgtggttcc
     1801 aacagttgga cggtggtaga cattgaccca cccctgaggt ccaacgaccc caaatcacag
     1861 aaccacccag ggtggctgat gcggggtctc aagccctgga cccagtatgc catctttgtg
     1921 aagaccctgg tcaccttttc ggatgaacgc cggacctatg gggccaagag tgacatcatt
     1981 tatgtccaga cagatgccac caacccctct gtgcccctgg atccaatctc agtgtctaac
     2041 tcatcatccc agattattct gaagtggaaa ccaccctccg accccaatgg caacatcacc
     2101 cactacctgg ttttctggga gaggcaggcg gaagacagtg agctgttcga gctggattat
     2161 tgcctcaaag ggctgaagct gccctcgagg acctggtctc caccattcga gtctgaagat
     2221 tctcagaagc acaaccagag tgagtatgag gattcggccg gcgaatgctg ctcctgtcca
     2281 aagacagact ctcagatcct gaaggagctg gaggagtcct cgtttaggaa gacgtttgag
     2341 gattacctgc acaacgtggt tttcgtcccc aggccatctc ggaaacgcag gtcccttggc
     2401 gatgttggga atgtgacggt ggccgtgccc acggtggcag ctttccccaa cacttcctcg
     2461 accagcgtgc ccacgagtcc ggaggagcac aggccttttg agaaggtggt gaacaaggag
     2521 tcgctggtca tctccggctt gcgacacttc acgggctatc gcatcgagct gcaggcttgc
     2581 aaccaggaca cccctgagga acggtgcagt gtggcagcct acgtcagtgc gaggaccatg
     2641 cctgaagcca aggctgatga cattgttggc cctgtgacgc atgaaatctt tgagaacaac
     2701 gtcgtccact tgatgtggca ggagccgaag gagcccaatg gtctgatcgt gctgtatgaa
     2761 gtgagttatc ggcgatatgg tgatgaggag ctgcatctct gcgtctcccg caagcacttc
     2821 gctctggaac ggggctgcag gctgcgtggg ctgtcaccgg ggaactacag cgtgcgaatc
     2881 cgggccacct cccttgcggg caacggctct tggacggaac ccacctattt ctacgtgaca
     2941 gactatttag acgtcccgtc aaatattgca aaaattatca tcggccccct catctttgtc
     3001 tttctcttca gtgttgtgat tggaagtatt tatctattcc tgagaaagag gcagccagat
     3061 gggccgctgg gaccgcttta cgcttcttca aaccctgagt atctcagtgc cagtgatgtg
     3121 tttccatgct ctgtgtacgt gccggacgag tgggaggtgt ctcgagagaa gatcaccctc
     3181 cttcgagagc tggggcaggg ctccttcggc atggtgtatg agggcaatgc cagggacatc
     3241 atcaagggtg aggcagagac ccgcgtggcg gtgaagacgg tcaacgagtc agccagtctc
     3301 cgagagcgga ttgagttcct caatgaggcc tcggtcatga agggcttcac ctgccatcat
     3361 gtggtgcgcc tcctgggagt ggtgtccaag ggccagccca cgctggtggt gatggagctg
     3421 atggctcacg gagacctgaa gagctacctc cgttctctgc ggccagaggc tgagaataat
     3481 cctggccgcc ctccccctac ccttcaagag atgattcaga tggcggcaga gattgctgac
     3541 gggatggcct acctgaacgc caagaagttt gtgcatcggg acctggcagc gagaaactgc
     3601 atggtcgccc atgattttac tgtcaaaatt ggagactttg gaatgaccag agacatctat
     3661 gaaacggatt actaccggaa agggggcaag ggtctgctcc ctgtacggtg gatggcaccg
     3721 gagtccctga aggatggggt cttcaccact tcttctgaca tgtggtcctt tggcgtggtc
     3781 ctttgggaaa tcaccagctt ggcagaacag ccttaccaag gcctgtctaa tgaacaggtg
     3841 ttgaaatttg tcatggatgg agggtatctg gatcaacccg acaactgtcc agagagagtc
     3901 actgacctca tgcgcatgtg ctggcaattc aaccccaaga tgaggccaac cttcctggag
     3961 attgtcaacc tgctcaagga cgacctgcac cccagctttc cagaggtgtc gttcttccac
     4021 agcgaggaga acaaggctcc cgagagtgag gagctggaga tggagtttga ggacatggag
     4081 aatgtgcccc tggaccgttc ctcgcactgt cagagggagg aggcgggggg ccgggatgga
     4141 gggtcctcgc tgggtttcaa gcggagctac gaggaacaca tcccttacac acacatgaac
     4201 ggaggcaaga aaaacgggcg gattctgacc ttgcctcggt ccaatccttc cgacccagct
     4261 ttcttgtaca aagtggttga tatcaaggcc gccgacatca ccagcctgta caagaaagtg
     4321 gggtggagcc atccccagtt cgagaagggc ggaggatctg gcggcggaag cggcggagga
     4381 agctggtccc accctcagtt tgagaaaggc accgagctgg gcagcaccat ggccagctac
     4441 ccctacgacg tgcccgacta cgccaaggac aacaccgtgc ccctgaagct gatcgccctg
     4501 ctggccaacg gcgagttcca ctctggcgag cagctgggcg agacactggg catgagcaga
     4561 gccgccatca acaagcacat ccagaccctg cgggactggg gcgtggacgt gttcacagtg
     4621 cccggcaagg gctacagcct gcccgagccc atccagctgc tgaacgccaa gcagatcctg
     4681 ggccagctgg atggcggatc tgtggccgtg ctgcccgtga tcgacagcac caaccagtat
     4741 ctgctcgacc ggatcggcga gctgaagtcc ggcgacgcct gtatcgccga gtaccagcag
     4801 gccggcagag gcggcagggg cagaaagtgg ttcagcccct tcggcgccaa cctgtacctg
     4861 agcatgttct ggagactgga acagggccca gccgccgcta ttggcctgag cctggtcatc
     4921 ggcatcgtga tggccgaggt gctgcggaag ctgggcgccg acaaagtgcg cgtgaagtgg
     4981 cccaacgacc tgtatctgca ggaccggaag ctggccggca tcctggtgga actgaccggc
     5041 aaaacaggcg acgccgccca gatcgtgatc ggcgctggca tcaacatggc catgcggcgg
     5101 gtggaagaga gcgtggtcaa ccagggctgg atcaccctgc aggaagccgg catcaacctg
     5161 gaccggaaca ccctggccgc catgctgatc agagagctga gagccgccct ggaactgttc
     5221 gagcaggaag gcctggcccc ctacctgagc agatgggaga agctggacaa cttcatcaac
     5281 cggcccgtga aactgatcat cggcgacaaa gagatcttcg gcatcagccg gggcatcgac
     5341 aagcaggggg ccctgctcct ggaacaggac ggcatcatca agccctggat gggcggcgag
     5401 atcagcctgc ggagcgccga gaagtgatga ctcgagtcta gagggcccgt ttaaacccgc
     5461 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5521 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5581 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5641 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5701 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5761 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5821 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5881 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5941 gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt
     6001 tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg
     6061 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     6121 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     6181 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     6241 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     6301 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     6361 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6421 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6481 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6541 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6601 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6661 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6721 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6781 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6841 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6901 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6961 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     7021 aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat
     7081 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7141 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7201 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7261 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     7321 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7381 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7441 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7501 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7561 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7621 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7681 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7741 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7801 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7861 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7921 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7981 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8041 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8101 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8161 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8221 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8281 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8341 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8401 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8461 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8521 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8581 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8641 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8701 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8761 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8821 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8881 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8941 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9001 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9061 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9121 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9181 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9241 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9301 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9361 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9421 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9481 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     9541 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9601 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9661 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9721 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     9781 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9841 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9901 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9961 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
    10021 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
    10081 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
    10141 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
    10201 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
    10261 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
    10321 atataagcag agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc
    10381 gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
//
