LOCUS       sequence-370714-       18342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed 
            Cbr-unc-119 marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18342)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 18342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS640
FEATURES             Location/Qualifiers
     source          1..18342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(399..417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        485..589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(590..658,659..1450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(808..827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1621..2209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2110..2129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    3726..3759
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             5718..5729
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5878..5911
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6065..6085
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6589..6613
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6625..6645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(16454..16478)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      16483..16806
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(16810..16830)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(16838..16855)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16838..16854)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgctgt gacgtcgtga ctggggaaaa ccctggcgtt acccaactta atcgccttgc
       61 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      121 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      181 tctgtgcggt atttcacacc gcatatggtg cactctcagt gcaatctgct ctgatgccgc
      241 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      301 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      361 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      421 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1321 tgcagcactg gggtcagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2221 cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc
     2281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg
     2341 ataacaattt cacagggccc tgtcggatta tgggctcttc ttttatcctc tactttcttt
     2401 ctagaaaatg caaatgttga gtcagaaatt gctgaatgga gtacaactgc aatgggttta
     2461 gcttatgttt ctggaacaat tactggtgga attgtgattg aaaagtaagg ttatatttca
     2521 tttcaataaa tgttaccaca gtataacaaa aacaagttac attgttcaga gtatgtctga
     2581 atgtattatt attattgtat ttgtgcatca gtactgtagt tgtactgaag aagggtttaa
     2641 aataatctca acgtttttaa gacgagttag tgagaatacg gtcgcagtta ggatgtttgg
     2701 acatagtttt attagataat cgtgaactaa ctcactataa aaacccgcta actcattgaa
     2761 taagatagtt ttaaacgttc aaaatttgaa gacaaaattg tatgaataag tttacagagt
     2821 tggtcgacgc aagcttctcc ttctcttcac atttctcaat aatttagctc taattgcatt
     2881 cgtattcttc gcaaaaatac gtattctgat tgacccgatg aaatacggat gcctagttgc
     2941 attgatcatc tacggttata cttatgggtg aggtttttca aatatatttt gatttaatcg
     3001 aataatgaaa tttcagaact ggagttggtc caatttcgtg gtttatatcc tctgaactgg
     3061 tacctcaaaa acatcgtagt attgctcaat ctgtggcata ttcaatgaat actgtaatgg
     3121 tggttatcag tacatttaca gtattacctc tatattccgt tattggtagt tatgctttcc
     3181 ttattctata ttcaattcct tcatttataa gtatgcttat attatttcgc tacttaccgg
     3241 aaaccaaagg acgagagatt catgatattg tgaatgaact taaaaaaaaa tgaaaacctt
     3301 tgaaaacgtt catacggccg aaattttact tgatcgagcc aattgaactt ttgttaagac
     3361 ttacagaaaa gtgagagatt ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga
     3421 aaaagaagaa aaatacaccc gaagtatcat ataaaaatta aaaagattgt ttgacctggc
     3481 ggaacttttt tgcaaaaact ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc
     3541 aaatgaatat caacaagaga ttcttgaaga cgacgagcca cttgctcaga gcattgctta
     3601 tctcgaatga gacccttgtt aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt
     3661 cctcctgatt ccatgatggt agcaaactca cttcgtagcg taagtttaaa catgatttta
     3721 ctaacataac ttcgtataaa gtatcctata cgaagttatg acattctcta atgaaaaaat
     3781 ctttcagttg aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt
     3841 gtgattagtg tttttagcga gtgtgacagg acagcgaaaa aatatagaaa caagggggga
     3901 actgaaaagc ttaggaatgc attgaacatg agaaggggaa agggaaggaa caaactagac
     3961 aggaattatt ggaatttaat cacatttgga gttttttttc tattcgacag aataattatc
     4021 cagaacattt ttgtattaaa tatttatgca tcatatgagt agtcggcttt gttgtgcatg
     4081 acgagtttgt tatcgacgaa atagaagctg tcagaacgag tctcgtttgg attgttgatc
     4141 atgtcgtcca ctgaaaaaga gattagtctt ttgaattgta ctttttagaa taatgactca
     4201 ctgagttgtt gagagagttg agggaactca tagatatgtt cacagttgtt tcgtgaattc
     4261 ggaatacaga atccgaattc aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga
     4321 cgttcgatca ttcggaaatg atggattggg atgtctccta ctttgaattc cacagttgct
     4381 ccgaccgttt tgagtttcaa aaagtttgga gcaaatctat aacgcacgta tcttgccgac
     4441 tcctgtggcg actcatcatt ctcctgatcg ttctccggtt tggcgatctc gaaaagcact
     4501 tgctcagtgt ccagatcacg gatttggaac ttggtgaact cgatgttata gatgttcgca
     4561 gatggggagc ataagaatcc taaatttatg ttttaaactg aaatccaaag ggagcaagat
     4621 accttgagtg attcccggaa gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc
     4681 aagctccgat tccgttgtga ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa
     4741 atatggaaaa gttcaacaaa aagaaaagag aaaagaatga aatcggatat caagagttag
     4801 ttgagcggtt tctctagttt tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt
     4861 ggaatcgatc gttgttgctc ggctttcata tcggtttggt tggaagcggc tgaaaacgga
     4921 aagaagtgga agaaggaaaa gagtgtggtg tgacaggaaa atggtaatta gagggtgcca
     4981 aataaccagc tatattttgt ttttttttga aaacattttt aaaaagaaaa atacgataat
     5041 gatatcagat ggatttccgg aaaactggta tgaaaaattt caaccttttt gagtacatgt
     5101 aatcaaaata cactttgtaa attatcattt ttattgaaac tccaccattt ttctatttat
     5161 aacgctaata atttgaaaaa gaaacctatt gcgaaccgcg gggtgaatcc caaaaacgaa
     5221 tgcgttttgg tggagtgatt gattcgaatc gaagaagaaa aagaagaaga cgtggaatag
     5281 agagctcact cttaaccgag cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg
     5341 tcttcttctt cttcttcttc gaatgattga cagaaatggg aaaaagagga agattgagaa
     5401 gggaaaaagg aaggagaaaa gaagcagaag aagacgtcag agaggagagg aacgagcgga
     5461 aaagcagcgg gcgcaagtca tagaagtagc agagctgggg agaagaagac actatccaag
     5521 aaaggaatga cgagagagta tgcaaagggg tatagggtgc agacagaata ggaacagaat
     5581 aacagatgat gagccaagaa gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt
     5641 gggtcaattt gagcatgatg aattgaaatc atcccttgtt gggagttaat aaccggtttg
     5701 ttatcagaaa ccctgtaata gaagggcgcc ctaactttga gccaattcat cccggtttct
     5761 gtcaaatata tcaaaaagtg gtcaactgac aaattgtttt tgatattata ataaacattt
     5821 tatccgttaa caattttcga atacttttta caaggacttg gataaattgg ctcaaagata
     5881 acttcgtata aagtatccta tacgaagtta ttaactaatc tgatttaaat tttcaggctc
     5941 acgcacgtgg tttaaactag aactaggccc gggctacgta atacgactca cttaaggcct
     6001 tgactagagg gtaccagagc tcacctaggc aggaacagct atgaccatga ttacgccaag
     6061 ctatcaactt tgtatagaaa agttgggttt tcacaacggc aaaatatcag tttttaaaaa
     6121 attaaaccat aaaacaaata atataaccca atttttacat caaaccacaa gaaaaaaata
     6181 catttgggcc cacggataaa gaaattaaaa aaatacattt tttaaaggcg caccgaatta
     6241 aaattcattt gggtcttacc gcgtataccg tactccgttt gtttgatcat ttttgtcagc
     6301 gctggcggtt gttttttcat ttcatttctg cttcaaagac gttttctcga ataatttttc
     6361 gtttattctc ttttttaaaa ttaatttcta gccgtaaatg ttataaattc acccatttaa
     6421 cgcaaatttc atggtaatct catggaaaaa tgcagtttct ttgttaaaga aagcttaaat
     6481 agcaaaaatt ccccgacttt ccccaaaatc ctgctcgatt ttccgttttc tcattgtatt
     6541 ctctcttaat taattttatc gataatcaat tgaatgtttc agacagagac aagtttgtac
     6601 aaaaaagcag gctcgatgac tgctccaaag aagaagcgta aggtaccggt agaaaaaatg
     6661 gacaagaagt actccatcgg actcgacatc ggaaccaact ccgtcggatg ggccgtcatc
     6721 accgacgagt acaaggtccc atccaagaag ttcaaggtcc tcggaaacac cgaccgtcac
     6781 tccatcaaga agaacctcat cggagccctc ctcttcgact ccggagagac cgccgaggcc
     6841 acccgtctca agcgtaccgc ccgtcgtcgt tacacccgtc gtaagaaccg tatctgctac
     6901 ctccaggaga tcttctccaa cgagatggcc aaggtcgacg actccttctt ccaccgtctc
     6961 gaggagtcct tcctcgtcga ggaggacaag aagcacgagc gtcacccaat cttcggaaac
     7021 atcgtcgacg aggtcgccta ccacgagaag tacccaacca tctaccacct ccgtaagaag
     7081 ctcgtcgact ccaccgacaa ggccgacctc cgtctcatct acctcgccct cgcccacatg
     7141 atcaagttcc gtggacactt cctcatcgag ggagacctca acccagacaa ctccgacgtc
     7201 gacaagctct tcatccagct cgtccagacc tacaaccagc tcttcgagga gaacccaatc
     7261 aacgcctccg gagtcgacgc caaggccatc ctctccgccc gtctctccaa gtcccgtcgt
     7321 ctcgagaacc tcatcgccca gctcccagga gagaagaaga acggactctt cggaaacctc
     7381 atcgccctct ccctcggact caccccaaac ttcaagtcca acttcgacct cgccgaggac
     7441 gccaagctcc agctctccaa ggacacctac gacgacgacc tcgacaacct cctcgcccag
     7501 atcggagacc agtacgccga cctcttcctc gccgccaaga acctctccga cgccatcctc
     7561 ctctccgaca tcctccgtgt aaggagttga acggctgaaa aatcgatatt ttgagcgaaa
     7621 aaagccggaa aaatggattt tatcggataa aatttgattt tttgagctga gaaatgcctt
     7681 tatagacgaa tttccttgtc aaattgttaa aaactgcaaa atttgccaaa aaaaaagcat
     7741 tttttatatt tttttttaca aaaaattcca caaaaatggc aaaatttgac taaaaatgcc
     7801 aattttgtcg ttttctccgt gccacagcgg ccgaaaatcg atttttaagc gattttcggg
     7861 tgaaaaagtg taaaaaccga ctgaaaatcc agctgaaaac gacaaaaacg gtaagtttta
     7921 gctaattttc accttttttt cgatattttt ttactaaaaa aaaaccgaaa aattgagttt
     7981 ttttcaaatt tctgcaaaaa ttctgcaatt ttagcaattt ccatactatt ttgacgccga
     8041 aaaagcttaa aaatatgatt tttagaggtt tttaatggaa aattcatgga tttttcaaat
     8101 ttttccacga aaatcattga aaaatttgaa aaaattgatt tcctattcaa agttctagct
     8161 aaaaactgca attttagcaa tttccatact attttagtac tgaaaaagcc taaaaatgtg
     8221 attttcagag gatttcaatg gaaaattcat ggatttttcc gatttttttc gatgattttc
     8281 actaaaaaat tggaaaaatt catcaatttc ctattgaaaa cctctaaaaa ttatattttt
     8341 tttgcttttc cgacgtcaaa atagtatgga aaatgctaaa attgcagaat ttttccagaa
     8401 atttgaagaa aaaactcaat tttttccgtt ttttcaatga ttttcatgaa aaaattggaa
     8461 aattttgtaa attttccatt gaaaacctct gaaaatttgg aaattttcga atttaaatgt
     8521 ttaaaaaaaa ttgaatattt tgcgagtttt tgaatttttt ttgagaattt ttgaattttt
     8581 ttcacccaaa attttttcaa tttttcaggt caacaccgag atcaccaagg ccccactctc
     8641 cgcctccatg atcaagcgtt acgacgagca ccaccaggac ctcaccctcc tcaaggccct
     8701 cgtccgtcag cagctcccag agaagtacaa ggagatcttc ttcgaccagt ccaagaacgg
     8761 atacgccgga tacatcgacg gaggagcctc ccaggaggag ttctacaagt tcatcaagcc
     8821 aatcctcgag aagatggacg gaaccgagga gctcctcgtc aagctcaacc gtgaggacct
     8881 cctccgtaag cagcgtacct tcgacaacgg atccatccca caccagatcc acctcggaga
     8941 gctccacgcc atcctccgtc gtcaggagga cttctaccca ttcctcaagg acaaccgtga
     9001 gaagatcgag aagatcctca ccttccgtat cccatactac gtcggaccac tcgcccgtgg
     9061 aaactcccgt ttcgcctgga tgacccgtaa gtccgaggag accatcaccc catggaactt
     9121 cgaggaggtc gtcgacaagg gagcctccgc ccagtccttc atcgagcgta tgaccaactt
     9181 cgacaagaac ctcccaaacg agaaggtcct cccaaagcac tccctcctct acgagtactt
     9241 caccgtctac aacgagctca ccaaggtcaa gtacgtcacc gagggaatgc gtaagccagc
     9301 cttcctctcc ggagagcaga agaaggccat cgtcgacctc ctcttcaaga ccaaccgtaa
     9361 ggtcaccgtc aagcagctca aggaggacta cttcaagaag atcgagtgct tcgactccgt
     9421 cgagatctcc ggagtcgagg accgtttcaa cgcctccctc ggaacctacc acgacctcct
     9481 caagatcatc aaggacaagg acttcctcga caacgaggag aacgaggaca tcctcgagga
     9541 catcgtcctc accctcaccc tcttcgagga ccgtgagatg atcgaggtgc tgaagctcga
     9601 aaatttggag cttttacacg agatttttga agaaaaagcc tgaaaatcga gagattttga
     9661 gctgtggaat tcgttgaaat tacataattc gcgcccgtaa ttctttaaaa aaacgctgaa
     9721 atttgcagat tttgccagaa agttgaagaa aaatctactt ttccggcatt ctctattatt
     9781 ttcatggaaa attagaaaaa tccatgaata aacagtgaaa attgctgatt ttacctgaaa
     9841 ttttgaaaaa aaaaaaaatc taaaatcaag gttttaagcc taaaaactgc agatttcagc
     9901 caaaattgtg aaaaaagctg attttttgct attttgagct tttccatggg gtggaaattg
     9961 ccaaaaatta cggaacattc acacaaattt gctgaataat ccaattttcc agtgttttct
    10021 agagtttccg tagaaatttt gaaaaaaccc agtaaatttt caatcggaaa acctctaaaa
    10081 acatcgtttc aggcctataa atagtaaaaa ttaccgattt tattcgaaaa tttaaggaaa
    10141 agtcgtgaaa aacgagattt taagcctcaa aactgcagat tctatctaaa attaaaaaaa
    10201 aaaaacataa atatccgaat ttttggagga aaagcctgaa aatcgatatt ttaagcctca
    10261 aaactgaaga tttcagccaa aaaaaaaaac agatttttcg ctattttatg tgccaaaaaa
    10321 ccacattccg agcttttcca gagtggaaat tgccaaaaat tacagaattt tcccagaaat
    10381 tagcaaaaaa aaaacccatt tttcaggcgt tttcaacgat tttcatagca aatttgaaaa
    10441 atccatgaat tttttaatta tagaaaaccc tctgaaaatt gcagttttaa gctattccag
    10501 cattcaaata gtatggaaat tgctaaaaat tgaagaattt tcccaaaaat ttgatgaaaa
    10561 cttatttttc tggcgttttc gatgattttc atggaatttc gagaaaatta atgaattttc
    10621 aattaaaaaa cgtctgcaaa atcacatttt ttaaagcttt ttcagcaaca aaatagtatg
    10681 gaaattgctt tttaaaaaag cggaattttt ggaaaaaagg ttaaattttc agtcagtttt
    10741 tgcacttttt gactgaaaat tcactgaaat tagggaaaaa ttccggaaaa atgatttttt
    10801 cgaattgtag ggcaaatttt gcaatttcaa gcaatttcca taatatttta atgctggaaa
    10861 agctcaaatt tacgttttaa tgggatttta gtgaaaaatt catggatttt ccgaaaaatt
    10921 tgcaggaaaa tcatagtaaa gacctcaaaa aatcaataaa aaatttaaaa aattggacaa
    10981 tcacatttct gcgtaaaatc tgcacttttt cagtaaaaaa taccgatttt ccgtgaaaat
    11041 tctcgatttt taatcgaaaa tctcgaaatt tttacaaatt tcagcaccaa aatcgttgaa
    11101 aattctgaaa atttgaattt tttttccttg ttttttgagt aaaatttgca ctttttcagt
    11161 aaagaatacc gattttccgt gaaaattctc ggtttttaat cgaaaatctc gaaattttta
    11221 caaatttcag ctccgaaata gctgaaaatt ggacaaaaat ttcaattttt gccctgtttt
    11281 ttgagtaaaa tctgcacttt ttagttgaaa aaaaacacgg attttgtagg aaaatttggg
    11341 aaaaatctcc aaattttcac aattttccag taaaaacagc aatttttaaa acggttttta
    11401 atcaaaaatc tttagaattt tccaaatttc agcaccaaaa aagctaaaaa tcgctgaaat
    11461 tttcgacaaa ttccaaattt ccaggagcgt ctcaagacct acgcccacct cttcgacgac
    11521 aaggtcatga agcagctcaa gcgtcgtcgt tacaccggat ggggacgtct ctcccgtaag
    11581 ctcatcaacg gaatccgtga caagcagtcc ggaaagacca tcctcgactt cctcaagtcc
    11641 gacggattcg ccaaccgtaa cgtaagttta aacatgattt tactaactaa ctaatctgat
    11701 ttaaattttc agttcatgca gctcatccac gacgactccc tcaccttcaa ggaggacatc
    11761 cagaaggccc aggtctccgg acagggagac tccctccacg agcacatcgc caacctcgcc
    11821 ggatccccag ccatcaagaa gggaatcctc cagaccgtca aggtcgtcga cgagctcgtc
    11881 aaggtcatgg gacgtcacaa gccagagaac atcgtcatcg agatggcccg tgagaaccag
    11941 accacccaga agggacagaa gaactcccgt gagcgtatga agcgtatcga ggagggaatc
    12001 aaggagctcg gatcccagat cctcaaggag cacccagtcg agaacaccca gctccagaac
    12061 gagaagctct acctctacta cctccagaac ggacgtgaca tgtacgtcga ccaggagctc
    12121 gacatcaacc gtctctccga ctacgacgtc gaccacatcg tcccacagtc cttcctcaag
    12181 gacgactcca tcgacaacaa ggtcctcacc cgttccgaca agaaccgtgg aaagtccgac
    12241 aacgtcccat ccgaggaggt cgtcaagaag atgaagaact actggcgtca gctcctcaac
    12301 gccaagctca tcacccagcg taagttcgac aacctcacca aggccgagcg tggaggactc
    12361 tccgagctcg acaaggccgg attcatcaag cgtcagctcg tcgagacccg tcagatcacc
    12421 aagcacgtcg cccagatcct cgactcccgt atgaacacca agtacgacga gaacgacaag
    12481 ctcatccgtg gttggttttt ccgagagaaa aacgctgaaa aatgccgaaa attttgaatt
    12541 tgcaggcttt ttaggtgcaa aagtacggaa aaatcggaaa aaaatcagga aaacatgggg
    12601 aaaatccaat tttccagaaa aaatgtttga aaaaattgaa aacttttgtt tttttagtaa
    12661 taaagtttta aaaaatggca aaaatagctg aaaacagcga aaaaaaaaaa tgtttttttc
    12721 aggaaaaaat ttgcaaaaaa atgaagagca acaataattc cctttttttt ctgtgttttt
    12781 ttcggaaaaa ttgagatcga aaacgaaaaa cagcgaaaat tcccaatttt cccagttttt
    12841 aaaacatttt tttgtaaaaa tttggaaaaa acgagttttt tcagaaaaaa atgtgaaaaa
    12901 aaaggtaaat taccaattta tgcttttttt tcgatcgagt aaaaaatcga tttttccaag
    12961 atttttcgcg aaaaattcaa aaaaaaagaa cgaaaattct cgattttcta ctttttttcg
    13021 aaaaaaaaag gaaattatag atttttccaa gatttttcgc gaaaaaattc gaaaaaaatg
    13081 tttttttcga aaatttcaga ttttctaatt ttttcagaag aattcgaaaa aaaaaatcaa
    13141 aaaattcgca atttttagat ttttttaaaa tctcaatttt ctataattat tttcctaaaa
    13201 aaatgtaatt aaaaaaccgc taaaaacggt aaattccaaa tttttgcatt ttttcaaaaa
    13261 aaatttgaaa aaaaaaatca gaaaattctc aaatctattc ccttttttta aaatttttcc
    13321 caaaccaaaa aaaaaaaacg gtaaattttc aaatttagca tttttccaaa attaaacata
    13381 aaaaaaaacg caaatttcca aatttcgttt ttttttcaaa aaagttgaaa aatccagaaa
    13441 ataccctctt gtccatgaaa aaatacgaaa attttcaata aaaaatcaat ttcttcttaa
    13501 aaaattaatt ctcgattttt ccatgaaaaa aaatcaaata ttaattaaat ttgaaaaaaa
    13561 aaagaattta tctaatagtt tcgttttttt tcagaggaaa aatttgaaaa aaaaaacagt
    13621 aaattctctt tctttttgaa aaaaaaaatt atcgattctt ccaagttttt tcccgaaaaa
    13681 atcgacaaaa aaacgagaat ttccgatttt ctattttttt tttcaaaaaa atgtgaaaaa
    13741 aaaaattaaa aaattccaat tttgcatttt tttttcaaaa aaaatttaaa aacaccgtat
    13801 aattcccgat ttttccatga aaaaatacga aaaattccaa taaaaatcaa ctattttaaa
    13861 aatttgccgt tttttcagag gtcaaggtca tcaccctcaa gtccaagctc gtctccgact
    13921 tccgtaagga cttccagttc tacaaggtcc gtgagatcaa caactaccac cacgcccacg
    13981 acgcctacct caacgccgtc gtcggaaccg ccctcatcaa gaagtaccca aagctcgagt
    14041 ccgagttcgt ctacggagac tacaaggtct acgacgtccg taagatgatc gccaagtccg
    14101 agcaggagat cggaaaggcc accgccaagt acttcttcta ctccaacatc atgaacttct
    14161 tcaagaccga gatcaccctc gccaacggag agatccgtaa gcgtccactc atcgagacca
    14221 acggagagac cggagagatc gtctgggaca agggacgtga cttcgccacc gtccgtaagg
    14281 tcctctccat gccacaggtc aacatcgtca agaagaccga ggtccagacc ggaggattct
    14341 ccaaggagtc catcctccca aagcgtaact ccgacaagct catcgcccgt aagaaggact
    14401 gggacccaaa gaagtacgga ggattcgact ccccaaccgt cgcctactcc gtcctcgtcg
    14461 tcgccaaggt cgagaaggga aagtccaaga agctcaagtc cgtcaaggag ctcctcggaa
    14521 tcaccatcat ggagcgttcc tccttcgaga agaacccaat cgacttcctc gaggccaagg
    14581 gatacaagga ggtcaagaag gtgcttacaa ttttctggag aaaaatattt taaaaaataa
    14641 cgaaaatgga ccgaattttt ggagaaaatt ttaaaaaaaa cgacattttt cccaaaaaat
    14701 tgaaaaattt ccgaaaaaac gttcaaaatt ttcaaaaaag gagcattttt cgaaaaaaaa
    14761 aacgataata cggaaatttt ttgattttcc agtttttttc tcaaagaaaa tcggaaaaag
    14821 gttttttttc tttaaaaacg tccaaaattt ttcgaaaaaa gagaaaattt tcgattttcc
    14881 gatttttttc agaaaaaaat tgaaaaaaaa agaaattttc cgattttctc agagaaagac
    14941 gaaaagacga tatttttcgg aaaaaagttg aaaaatcaaa aaaaaaaccg aaaaacggcc
    15001 gaaattttct aaaaattgga aatattgtcg atttttcgaa aaaaaagagt caaaagtttt
    15061 caaaaaaata tattttttcg attttccgtt ttttttttca gtaaacattg aaacaaacga
    15121 aatttttcca aaaaaatcca attattttcc caaatatcga aatatttttg attttccgtt
    15181 tttttcagaa aaaagttaaa aaaaaaaggg aaaattaaga ttttcctgat tttttttgag
    15241 aaaaaatcga taaaaattga atattctgga aaaaaccttt aaaaaattcg aattaaatcg
    15301 aaaaacgtcc aaaattttct aaaaaattga aatttttttt gaaaaaaaac ggaaaaattt
    15361 agatttcccg acttttttca atgaaaaatc gaattttcgt tgacttttca ttaaaaaatc
    15421 caaacgcatt tttttccact aaaatctcga aaaagtgtca tattgcacca tattgtttcc
    15481 acattcatat tattttgagc taaatcaggt gattttctgt attttgatac taatttctat
    15541 ttttagaaaa aatgtgggaa caatatggtg caatatgaca ttttttcgag attttagtgg
    15601 agaaaaaatg cgtttggaaa cgaattttat aataaaaagt caacgaaaat tcgatttttc
    15661 atggaaaaat agggaaatcg aaaacaaatt tgcttttttt tcaaaaaaaa aaacggaaat
    15721 tcgaaatttt tccgttcttt caaaaaaaaa atcgattttt ttggaaattt tttttttcaa
    15781 atttttaaaa atttccagga cctcatcatc aagctcccaa agtactccct cttcgagctc
    15841 gagaacggac gtaagcgtat gctcgcctcc gccggagagc tccagaaggg aaacgagctc
    15901 gccctcccat ccaagtacgt caacttcctc tacctcgcct cccactacga gaagctcaag
    15961 ggatccccag aggacaacga gcagaagcag ctcttcgtcg agcagcacaa gcactacctc
    16021 gacgagatca tcgagcagat ctccgagttc tccaagcgtg tcatcctcgc cgacgccaac
    16081 ctcgacaagg tcctctccgc ctacaacaag caccgtgaca agccaatccg tgagcaggcc
    16141 gagaacatca tccacctctt caccctcacc aacctcggag ccccagccgc cttcaagtac
    16201 ttcgacacca ccatcgaccg taagcgttac acctccacca aggaggtcct cgacgccacc
    16261 ctcatccacc agtccatcac cggactctac gagacccgta tcgacctctc ccagctcgga
    16321 ggagactctg gaggatctgg aggaactgga ggatcttccg ccggcgggag ctctatgagc
    16381 cgtagacgaa aagcgaatcc gacaaaactg agtgaaaacg cgaagaagct tgccaaggaa
    16441 gttgaaaatt aacacccagc tttcttgtac aaagtgggat aaatgcaaaa tcctttcaag
    16501 cattcccttc ttctctatca ctcttctttc tttttgtcaa aaaattctct cgctaattta
    16561 tttgcttttt taatgttatt attttatgac tttttatagt cactgaaaag tttgcatctg
    16621 agtgaagtga atgctatcaa aatgtgattc tgtctgatgt actttcacaa tctctcttca
    16681 attccatttt gaagtgcttt aaacccgaaa ggttgagaaa aatgcgagcg ctcaaatatt
    16741 tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac tttattgtgc cgccaagaaa
    16801 aaagtctcac aactttatta tacatagttg ataattcact ggccgtcgtt ttacactcga
    16861 gacgtacggt gcgcgcgatg cattcgaaga tctgcccact agtgagtcgt attataagtg
    16921 caagtaagat cagtgtttgt ttcgatatca gtctgtttcg taacggtctt ctgtataact
    16981 acaaaaaaga ataaaaaacc gtatcacaac tgagaaaaaa gacaaaactt attgaacagt
    17041 gttcctttca aaaaattatt ttgtaaaaaa tcgagttttt ctccaaaata acgcacgcaa
    17101 tcaaaaacaa gaaaactaac gtgttctcct gggcgtggaa ctccttatca agattaccat
    17161 tattgtctgt cagaattgat atttcaatta tttcatatct agacaaaccc gggtgtcttg
    17221 catttattag tactgtgagt ttacatacaa acgaggctta ctcagataac atagagtcac
    17281 ctctaattag caaaaaccca tttctaatta gctatttcga ccagttctaa agtcaccgta
    17341 attagaaatg gtttttgtag cttttctaat tagaaatgac cccgagataa gtgaaatatg
    17401 gcattttctg ggttttctag taagaaatga tcccgcacgt agtgattccg cgttacaagc
    17461 caaattcaaa ttaaaaaagt atattacaca attcaagtta cattattttc aggaaacaat
    17521 ggcgaagagc aaagcagaga ttcaaaagga ggaaagagac aacagatatg aacgaagact
    17581 tcttgaaaag agaaagagac atgaggtgag taaccatgat aataaataat caagtaaggg
    17641 ctatagattt ataaattctg gagttaacag tttcgaaaaa aagtagtttt catatgtgcg
    17701 aaggaatatc aaaaataggg gaatagccta aacgagtgag aaaattaaat gtttgttatt
    17761 ttttcaaaaa tgtctgcagg gccgttatcc tttcaaatgc actctatcaa acatagatca
    17821 tatccatcat atcttatcca gaatttatat catatccaag attgagagcg tttcaaggaa
    17881 aatctcaaac aaccatacaa ttttgtagat cgacaacaag gcctccactt cttcatcgat
    17941 ggcctctgca gcacctgcac caccaagttt gtatttttca tattaatata aagtgagcca
    18001 cgcatagtcg tttttaggat ttttgccaga tcgatgtcgc gcaaacagac tgtttttttt
    18061 tctttgaaag ccgtgcgtga catgggccac ggattgtaac gccgcgctgg atggataaaa
    18121 atttattcag ttttttagcc tttacaaaat atggttcgac tactttcttt atttttacat
    18181 ttttataggt gctcattgag ctgagatatt ttgggtataa tcggaaaatc gctgattttg
    18241 gtcatttttc tgcactaaag attacacaaa aagttcagta tttagattca ttgttgaaaa
    18301 ttggggatga tctacagata catatcgccg tgttcaaaaa ca
//
