LOCUS       sequence-37074-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Shan Y, Napoli E, Cortopassi G
  TITLE     Mitochondrial frataxin interacts with ISD11 of the NFS1/ISCU complex
            and multiple mitochondrial chaperones.
  JOURNAL   Hum Mol Genet. 2007 Apr 15;16(8):929-41. Epub 2007 Mar 1.
  PUBMED    17331979
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet."; date: "2007-04-15"; volume: "16(8)"; pages: "929-41. Epub 
            2007 Mar 1"
COMMENT     Sequence Label: p3xFlag-CMV14-hISD11
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(185..250)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(594..693)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(623..643)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttccacttgc cccttgctcc ataccacccc cctccacccc ataatattat agaaggacac
       61 ctagtcagac aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt
      121 ggcaacttcc agggccagga gaggcactgg ggaggggtca cagggatgcc acccgggatc
      181 actacttgtc atcgtcatcc ttgtagtcga tgtcatgatc tttataatca ccgtcatggt
      241 ctttgtagtc agcccgggat cctctagagt cgactggtac cgaggtcctg ggcatgtctc
      301 gattctcaat gatcagcttg tcagttgaat acagttggcc aatgtggacc tgtcgacgaa
      361 ttactccaag gtctctcttg gctttattca ctagggtttg aatttctaca ggatccttta
      421 catttttatt ttctctgaag gcatctctta tcctcctgac agcatatgtt ctgtaattgt
      481 aggcgctgaa acgcttgctc tctctcagca tcgcccggta cagagctaac acttgtgcgc
      541 gactggaggc tgccattgcg gccgcaagct taattctgac ggttcactaa acgagctctg
      601 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaacgg ggcggggtta
      661 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
      721 atgg
//
