LOCUS       sequence-37075-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Wright KJ, Tjian R
  TITLE     Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):55-60. Epub 2008 Dec 30.
  PUBMED    19116271
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-01-6"; volume: "106(1)"; pages: 
            "55-60. Epub 2008 Dec 30"
COMMENT     Sequence Label: pMT-Pygopus
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..20
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(43..60)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(70..111)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(294..720)
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
ORIGIN
        1 cttagaaggc acagtcgagg ctgatcagcg ggtttaaact caatggtgat ggtgatgatg
       61 accggtacgc gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg
      121 cgggccctct agactcgagc ggccgccact gtgctggata tctgcagaat tgcccttaca
      181 cttgaacttg accatgggaa tatgctgtac aaaagggccc gccataccaa gattgtgggt
      241 cataagggca attccaccac actggactag taggtacccc gatctagatc ccccctttag
      301 ttgcactgag atgattcact tgattttgct gctgaccaca actgatgcac attggccaga
      361 tgctctcggt tttatacatt ttggaaacgc ggctttacac acgggtcttt cgttcgattc
      421 acctcttttg cacacgccgg ccggctccaa attcgagtgg agacgtgtgc agaagctttt
      481 gttctgcccg cgaagaattg gttcggttct ccactttggg gacttgtatg ggatgcaaag
      541 ctcttggtgg ggcacatggg gcatgcgcag gcactcttac ttgcgtacca catatgtatg
      601 ggggtggcgg tgggcggtgg ggggccggag ttctgggtct cttatcggaa ccagaggata
      661 ggacggcctg cagcaaagag ctagtcacat cgggcaccac atcctgtcct gcaacgaatt
      721 aattcactgg ccgtcgttta caacgtcg
//
