LOCUS       sequence-370756-       13179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one prime editor piggyBac transposon with PEmax, SpG variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, 
            Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin 
            U, Petri S, Cantz T
  TITLE     A selectable all-in-one CRISPR prime editing piggyBac transposon 
            allows for highly efficient gene editing in human cell lines.
  JOURNAL   Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2.
  PUBMED    34773059
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-11-12"; volume: "11(1):22154. doi"; pages: " 
            10.1038/s41598-021-01689-2"
COMMENT     Sequence Label: PB-PEmax SpG
FEATURES             Location/Qualifiers
     source          1..13179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..265
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     17..37
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     188..207
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        294..369
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        415..794
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        796..1071
                     /label=chicken beta-actin promoter
     intron          1072..2088
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2012..2034
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2096..2115
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2154..2173
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2154..2172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2222..2242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2243..6343
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6401..6421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8519..8539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8549..8575
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8585..8641
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8642..9238
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8642..8661)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9138..9158
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9245..10240
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(10262..10279)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10268..10492
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10676..10738
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(10804..11392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10884..10903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11563..12354,12355..12423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12186..12205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12424..12528)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12596..12614
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12652..12674)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12774..12793
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     repeat_region   12817..12851
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccgggtc ttcgtggagc tcatcgaaga cctgttttag
      301 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      361 agtcggtgca gagaccgaat cgaccagagg tctcgttttt ttctagacgc tagcgacatt
      421 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      781 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      841 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      901 cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      961 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     1021 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
     1081 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     1141 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     1201 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     1261 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     1321 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     1381 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1441 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     1501 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     1561 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     1621 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1681 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
     1741 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     1801 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     1861 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     1921 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1981 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     2041 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     2101 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgatagcggc cgctaatacg
     2161 actcactata gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc
     2221 accaaagaag aagcggaaag tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa
     2281 ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt
     2341 gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga
     2401 cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag
     2461 acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga
     2521 cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga
     2581 gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac
     2641 catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat
     2701 ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct
     2761 gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca
     2821 gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc
     2881 cagactgagc aagagcagaa agctggaaaa tctgatcgcc cagctgcccg gcgagaagaa
     2941 gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag
     3001 caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga
     3061 cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa
     3121 gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa
     3181 ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct
     3241 gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca
     3301 gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa
     3361 gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa
     3421 gagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat
     3481 ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa
     3541 ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc
     3601 tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac
     3661 cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg
     3721 gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct
     3781 gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat
     3841 gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa
     3901 gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg
     3961 cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata
     4021 ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga
     4081 cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga
     4141 acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg
     4201 gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca
     4261 gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat
     4321 gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc
     4381 cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa
     4441 gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca
     4501 caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca
     4561 gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca
     4621 gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta
     4681 ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc
     4741 cgactacgat gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgacaa
     4801 caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga
     4861 ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca
     4921 gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc
     4981 cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat
     5041 cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa
     5101 agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa
     5161 agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg
     5221 aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa
     5281 ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc
     5341 caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa
     5401 cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg
     5461 ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat
     5521 cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag
     5581 gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt
     5641 cctgtggccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc
     5701 caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt
     5761 cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct
     5821 gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct
     5881 ggcctctgcc aagcagctgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa
     5941 cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca
     6001 gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag
     6061 cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta
     6121 caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac
     6181 cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa
     6241 gcagtacaga agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg
     6301 cctgtacgag acacggatcg acctgtctca gctgggaggc gactctggag gatctagcgg
     6361 aggatccaag cggaccgccg acggctctga gttcgagagc cctaagaaga aaagaaaggt
     6421 gagcggaggc tctagcggcg gaagcaccct gaacattgag gacgagtata gactgcatga
     6481 aacaagcaag gaacccgacg tgtccctggg ctccacctgg ctgtccgact ttccccaggc
     6541 ctgggccgag acaggaggaa tgggcctggc cgtgcggcag gcacccctga tcatccctct
     6601 gaaggccacc tctacacccg tgagcatcaa gcagtaccct atgtctcagg aggccagact
     6661 gggcatcaag cctcacatcc agaggctgct ggaccagggc atcctggtgc catgccagag
     6721 cccctggaac acaccactgc tgcccgtgaa gaagccaggc accaatgact atagacccgt
     6781 gcaggatctg agagaggtga acaagagggt ggaggatatc caccccaccg tgcccaaccc
     6841 ttacaatctg ctgtccggcc tgcccccttc tcaccagtgg tatacagtgc tggacctgaa
     6901 ggatgccttc ttttgtctga gactgcaccc taccagccag ccactgttcg cctttgagtg
     6961 gagggaccct gagatgggca tctctggcca gctgacctgg acacgcctgc ctcagggctt
     7021 caagaatagc ccaacactgt ttaacgaggc cctgcaccgc gacctggcag atttccgcat
     7081 ccagcaccca gatctgatcc tgctgcagta cgtggacgat ctgctgctgg ccgccaccag
     7141 cgagctggat tgccagcagg gaacacgcgc cctgctgcag accctgggaa acctgggata
     7201 tagggcatcc gccaagaagg cccagatctg tcagaagcag gtgaagtacc tgggctatct
     7261 gctgaaggag ggccagagat ggctgacaga ggccaggaag gagacagtga tgggccagcc
     7321 aacacccaag accccaagac agctgaggga gttcctgggc aaagcaggat tttgcaggct
     7381 gttcatccca ggattcgcag agatggcagc acctctgtac ccactgacca agccgggcac
     7441 cctgtttaat tggggccctg accagcagaa ggcctatcag gagatcaagc aggccctgct
     7501 gacagcacca gccctgggcc tgccagacct gaccaagcct ttcgagctgt ttgtggatga
     7561 gaagcagggc tacgccaagg gcgtgctgac ccagaagctg ggaccatgga gacggcccgt
     7621 ggcctatctg tccaagaagc tggacccagt ggcagcagga tggccaccat gcctgaggat
     7681 ggtggcagca atcgccgtgc tgacaaagga tgccggcaag ctgaccatgg gacagccact
     7741 ggtcatcctg gcaccacacg cagtggaggc cctggtgaag cagcctccag atcgctggct
     7801 gtctaacgcc cggatgacac actaccaggc cctgctgctg gacaccgatc gcgtgcagtt
     7861 tggccctgtg gtggccctga atccagccac cctgctgcct ctgccagagg agggcctgca
     7921 gcacaactgt ctggacatcc tggcagaggc acacggaaca aggccagacc tgaccgatca
     7981 gcccctgcct gacgccgatc acacatggta taccgatgga agctccctgc tgcaggaggg
     8041 ccagaggaag gcaggagcag cagtgaccac agagacagaa gtgatctggg ccaaggccct
     8101 gccagcaggc acatccgccc agcgggccga gctgatcgcc ctgacccagg ccctgaagat
     8161 ggccgagggc aagaagctga acgtgtacac agactccaga tatgccttcg ccaccgcaca
     8221 catccacgga gagatctaca ggcgccgggg ctggctgacc tctgagggca aggagatcaa
     8281 gaacaaggat gagatcctgg ccctgctgaa ggccctgttt ctgcccaagc ggctgagcat
     8341 catccactgt cctggacacc agaagggaca ctccgccgag gcaaggggca atcggatggc
     8401 cgaccaggcc gccagaaagg ctgctattac tgaaactccc gacacttcca ctctgctgat
     8461 tgaaaactcc tccccttctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
     8521 caagaagaag aggaaagtcg gctctggccc tgccgctaag agagtgaagc tggacggctc
     8581 cggcgctacc aatttctccc tgctcaagca agccggagac gtcgaggaaa acccaggccc
     8641 aatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     8701 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8761 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8821 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8881 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8941 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     9001 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     9061 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9121 ggagacatcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9181 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg
     9241 ctccatgccc acgctactgc gggtttatat agacggtcct cacgggatgg ggaaaaccac
     9301 caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat atcgtctacg tacccgagcc
     9361 gatgacttac tggcaggtgc tgggggcttc cgagacaatc gcgaacatct acaccacaca
     9421 acaccgcctc gaccagggtg agatatcggc cggggacgcg gcggtggtaa tgacaagcgc
     9481 ccagataaca atgggcatgc cttatgccgt gaccgacgcc gttctggctc ctcatatcgg
     9541 gggggaggct gggagctcac atgccccgcc cccggccctc accctcatct tcgaccgcca
     9601 tcccatcgcc gccctcctgt gctacccggc cgcgcgatac cttatgggca gcatgacccc
     9661 ccaggccgtg ctggcgttcg tggccctcat cccgccgacc ttgcccggca caaacatcgt
     9721 gttgggggcc cttccggagg acagacacat cgaccgcctg gccaaacgcc agcgccccgg
     9781 cgagcggctt gacctggcta tgctggccgc gattcgccgc gtttacgggc tgcttgccaa
     9841 tacggtgcgg tatctgcagg gcggcgggtc gtggcgggag gattggggac agctttcggg
     9901 gacggccgtg ccgccccagg gtgccgagcc ccagagcaac gcgggcccac gaccccatat
     9961 cggggacacg ttatttaccc tgtttcgggc ccccgagttg ctggccccca acggcgacct
    10021 gtacaacgtg tttgcctggg ccttggacgt cttggccaaa cgcctccgtc ccatgcacgt
    10081 ctttatcctg gattacgacc aatcgcccgc cggctgccgg gacgccctgc tgcaacttac
    10141 ctccgggatg gtccagaccc acgtcaccac ccccggctcc ataccgacga tctgcgacct
    10201 ggcgcgcacg tttgcccggg agatggggga ggctaactga gctctagagc tcgctgatca
    10261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    10321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    10381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    10441 gaggattggg aggacaatag caggcatgct ggggatgcgg tgggctctat ggggcgcgcc
    10501 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
    10561 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
    10621 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
    10681 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
    10741 aaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
    10801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
    10861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
    10921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
    10981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
    11041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
    11101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
    11161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
    11221 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
    11281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
    11341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
    11401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
    11461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
    11521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    11581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    11641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    11701 gcgactccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    11761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    11821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    11881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    11941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    12001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    12061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    12121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    12181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    12241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    12301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    12361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    12421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    12481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    12541 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    12601 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    12661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    12721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    12781 agagcagatt gtactgagag tgcaccaatt tgttaaccct agaaagatag tctgcgtaaa
    12841 attgacgcat gcattcttga aatattgctc tctctttcta aatagcgcga atccgtcgct
    12901 gtgcatttag gacatctcag tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg
    12961 gtaagtgtca ctgattttga actataacga ccgcgtgagt caaaatgacg catgattatc
    13021 ttttacgtga cttttaagat ttaactcata cgataattat attgttattt catgttctac
    13081 ttacgtgata acttattata tatatatttt cttgttatag atatcatgta caaaaaagca
    13141 ggctttaaag gaaccaattc agtcgactgg acccggtac
//
