LOCUS       sequence-370764-       10529 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human RON.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10529)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_RON
FEATURES             Location/Qualifiers
     source          1..10529
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..40)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(194..782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(274..293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(953..1744,1745..1813))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1576..1595
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1814..1918)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1993..2012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2184..2563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2564..2767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2718..2738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2769..2787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2790..2808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2818..2842
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2985..3009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7215..7239)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7286..7309
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7346..7369
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7400..7426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7400..7426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7427..7774,7775..7777,7778..8386)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8430..8447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8436..8660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8793..8812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8944..8991
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8999..10021
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8999..10021
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10151..10272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10188..10207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10242..10261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10321..10337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10321..10337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10334..10356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10345..10361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10369..10375,10376..10393,10394..10399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10414..10435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
       61 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      121 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      181 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      241 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      301 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      361 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      421 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      481 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      541 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      601 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
      661 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
      721 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
      781 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
      841 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
      901 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      961 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1021 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1081 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     1141 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     1201 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     1261 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     1321 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     1381 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     1441 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     1501 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     1561 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     1621 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     1681 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     1741 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     1801 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1861 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1921 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     1981 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     2041 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     2101 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     2161 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     2221 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2281 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2341 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2401 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2461 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2521 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2581 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2641 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2701 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2761 cagagctctc cctatcagtg atagagatct ccctatcagt gatagagatc gtcgacgagc
     2821 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     2881 aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc ttggtaccga
     2941 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa
     3001 aagcaggctc caccatggag tcctcccgcc gctgcctcag tccttcctgt tgctgctgct
     3061 gttgcctgcc aagcccgcgg cgggcgagga ctggcagtgc ccgcgcaccc cctacgcggc
     3121 ctctcgcgac tttgacgtga agtacgtggt gcccagcttc tccgccggag gcctggtaca
     3181 ggccatggtg acctacgagg gcgacagaaa tgagagtgct gtgtttgtag ccatacgcaa
     3241 tcgcctgcat gtgcttgggc ctgacctgaa gtctgtccag agcctggcca cgggccctgc
     3301 tggagaccct ggctgccaga cgtgtgcagc ctgtggccca ggaccccacg gccctcccgg
     3361 tgacacagac acaaaggtgc tggtgctgga tcccgcgctg cctgcgctgg tcagttgtgg
     3421 ctccagcctg cagggccgct gcttcctgca tgacctagag ccccaaggga cagccgtgca
     3481 tctggcagcg ccagcctgcc tcttctcagc ccaccataac cggcccgatg actgccccga
     3541 ctgtgtggcc agcccattgg gcacccgtgt aactgtggtt gagcaaggcc aggcctccta
     3601 tttctacgtg acatcctcac tggacgcagc cgtggctgcc agcttcagcc cacgctcagt
     3661 gtctatcagg cgtctcaagg ctgacgcctc gggattcgca ccgggctttg tggcgttgtc
     3721 agtgctgccc aagcatcttg tctcctacag tattgaatac gtgcacagct tccacacggg
     3781 agccttcgta tacttcctga ctgtacagcc ggccagcgtg acagatgatc ctagtgccct
     3841 gcacacacgc ctggcacggc ttagcgccac tgagccagag ttgggtgact atcgggagct
     3901 ggtcctcgac tgcagatttg ctccaaaacg caggcgccgg ggggccccag aaggcggaca
     3961 gccctaccct gtgctgcggg tggcccactc cgctccagtg ggtgcccaac ttgccactga
     4021 gctgagcatc gccgagggcc aggaagtact atttggggtc tttgtgactg gcaaggatgg
     4081 tggtcctggc gtgggcccca actctgtcgt ctgtgccttc cccattgacc tgctggacac
     4141 actaattgat gagggtgtgg agcgctgttg tgaatcccca gtccatccag gcctccggcg
     4201 aggcctcgac ttcttccagt cgcccagttt ttgccccaac ccgcctggcc tggaagccct
     4261 cagccccaac accagctgcc gccacttccc tctgctggtc agtagcagct tctcacgtgt
     4321 ggacctattc aatgggctgt tgggaccagt acaggtcact gcattgtatg tgacacgcct
     4381 tgacaacgtc acagtggcac acatgggcac aatggatggg cgtatcctgc aggtggagct
     4441 ggtcaggtca ctaaactact tgctgtatgt gtccaacttc tcactgggtg acagtgggca
     4501 gcccgtgcag cgggatgtca gtcgtcttgg ggaccaccta ctctttgcct ctggggacca
     4561 ggttttccag gtacctatcc aaggccctgg ctgccgccac ttcctgacct gtgggcgttg
     4621 cctaagggca tggcatttca tgggctgtgg ctggtgtggg aacatgtgcg gccagcagaa
     4681 ggagtgtcct ggctcctggc aacaggacca ctgcccacct aagcttactg agttccaccc
     4741 ccacagtgga cctctaaggg gcagtacaag gctgaccctg tgtggctcca acttctacct
     4801 tcacccttct ggtctggtgc ctgagggaac ccatcaggtc actgtgggcc aaagtccctg
     4861 ccggccactg cccaaggaca gctcaaaact cagaccagtg ccccggaaag actttgtaga
     4921 ggagtttgag tgtgaactgg agcccttggg cacccaggca gtggggccta ccaacgtcag
     4981 cctcaccgtg actaacatgc caccgggcaa gcacttccgg gtagacggca cctccgtgct
     5041 gagaggcttc tctttcatgg agccagtgct gatagcagtg caacccctct ttggcccacg
     5101 ggcaggaggc acctgtctca ctcttgaagg ccagagtctg tctgtaggca ccagccgggc
     5161 tgtgctggtc aatgggactg agtgtctgct agcacgggtc agtgaggggc agcttttatg
     5221 tgccacaccc cctggggcca cggtggccag tgtccccctt agcctgcagg tggggggtgc
     5281 ccaggtacct ggttcctgga ccttccagta cagagaagac cctgtcgtgc taagcatcag
     5341 ccccaactgt ggctacatca actcccacat caccatctgt ggccagcatc taacttcagc
     5401 atggcactta gtgctgtcat tccatgacgg gcttagggca gtggaaagca ggtgtgagag
     5461 gcagcttcca gagcagcagc tgtgccgcct tcctgaatat gtggtccgag acccccaggg
     5521 atgggtggca gggaatctga gtgcccgagg ggatggagct gctggcttta cactgcctgg
     5581 ctttcgcttc ctacccccac cccatccacc cagtgccaac ctagttccac tgaagcctga
     5641 ggagcatgcc attaagtttg agtatattgg gctgggcgct gtggctgact gtgtgggtat
     5701 caacgtgacc gtgggtggtg agagctgcca gcacgagttc cggggggaca tggttgtctg
     5761 ccccctgccc ccatccctgc agcttggcca ggatggtgcc ccattgcagg tctgcgtaga
     5821 tggtgaatgt catatcctgg gtagagtggt gcggccaggg ccagatgggg tcccacagag
     5881 cacgctcctt ggtatcctgc tgcctttgct gctgcttgtg gctgcactgg cgactgcact
     5941 ggtcttcagc tactggtggc ggaggaagca gctagttctt cctcccaacc tgaatgacct
     6001 ggcatccctg gaccagactg ctggagccac acccctgcct attctgtact cgggctctga
     6061 ctacagaagt ggccttgcac tccctgccat tgatggtctg gattccacca cttgtgtcca
     6121 tggagcatcc ttctccgata gtgaagatga atcctgtgtg ccactgctgc ggaaagagtc
     6181 catccagcta agggacctgg actctgcgct cttggctgag gtcaaggatg tgctgattcc
     6241 ccatgagcgg gtggtcaccc acagtgaccg agtcattggc aaaggccact ttggagttgt
     6301 ctaccacgga gaatacatag accaggccca gaatcgaatc caatgtgcca tcaagtcact
     6361 aagtcgcatc acagagatgc agcaggtgga ggccttcctg cgagaggggc tgctcatgcg
     6421 tggcctgaac cacccgaatg tgctggctct cattggtatc atgttgccac ctgagggcct
     6481 gccccatgtg ctgctgccct atatgtgcca cggtgacctg ctccagttca tccgctcacc
     6541 tcagcggaac cccaccgtga aggacctcat cagctttggc ctgcaggtag cccgcggcat
     6601 ggagtacctg gcagagcaga agtttgtgca cagggacctg gctgcgcgga actgcatgct
     6661 ggacgagtca ttcacagtca aggtggctga ctttggtttg gcccgcgaca tcctggacag
     6721 ggagtactat agtgttcaac agcatcgcca cgctcgccta cctgtgaagt ggatggcgct
     6781 ggagagcctg cagacctata gatttaccac caagtctgat gtgtggtcat ttggtgtgct
     6841 gctgtgggaa ctgctgacac ggggtgcccc accataccgc cacattgacc cttttgacct
     6901 tacccacttc ctggcccagg gtcggcgcct gccccagcct gagtattgcc ctgattctct
     6961 gtaccaagtg atgcagcaat gctgggaggc agacccagca gtgcgaccca ccttcagagt
     7021 actagtgggg gaggtggagc agatagtgtc tgcactgctt ggggaccatt atgtgcagct
     7081 gccagcaacc tacatgaact tgggccccag cacctcgcat gagatgaatg tgcgtccaga
     7141 acagccgcag ttctcaccca tgccagggaa tgtacgccgg ccccggccac tctcagagcc
     7201 tcctcggccc actgacccag ctttcttgta caaagtggtt gatatcaagg ccgccgacat
     7261 caccagcctg tacaagaaag tggggtggag ccatccccag ttcgagaagg gcggaggatc
     7321 tggcggcgga agcggcggag gaagctggtc ccaccctcag tttgagaaag gcaccgagct
     7381 gggcagcacc atggccagct acccctacga cgtgcccgac tacgccaagg acaacaccgt
     7441 gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg agcagctggg
     7501 cgagacactg ggcatgagca gagccgccat caacaagcac atccagaccc tgcgggactg
     7561 gggcgtggac gtgttcacag tgcccggcaa gggctacagc ctgcccgagc ccatccagct
     7621 gctgaacgcc aagcagatcc tgggccagct ggatggcgga tctgtggccg tgctgcccgt
     7681 gatcgacagc accaaccagt atctgctcga ccggatcggc gagctgaagt ccggcgacgc
     7741 ctgtatcgcc gagtaccagc aggccggcag aggcggcagg ggcagaaagt ggttcagccc
     7801 cttcggcgcc aacctgtacc tgagcatgtt ctggagactg gaacagggcc cagccgccgc
     7861 tattggcctg agcctggtca tcggcatcgt gatggccgag gtgctgcgga agctgggcgc
     7921 cgacaaagtg cgcgtgaagt ggcccaacga cctgtatctg caggaccgga agctggccgg
     7981 catcctggtg gaactgaccg gcaaaacagg cgacgccgcc cagatcgtga tcggcgctgg
     8041 catcaacatg gccatgcggc gggtggaaga gagcgtggtc aaccagggct ggatcaccct
     8101 gcaggaagcc ggcatcaacc tggaccggaa caccctggcc gccatgctga tcagagagct
     8161 gagagccgcc ctggaactgt tcgagcagga aggcctggcc ccctacctga gcagatggga
     8221 gaagctggac aacttcatca accggcccgt gaaactgatc atcggcgaca aagagatctt
     8281 cggcatcagc cggggcatcg acaagcaggg ggccctgctc ctggaacagg acggcatcat
     8341 caagccctgg atgggcggcg agatcagcct gcggagcgcc gagaagtgat gactcgagtc
     8401 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8461 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8521 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8581 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8641 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8701 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8761 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8821 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8881 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtac
     8941 ctagaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt ccttggccaa
     9001 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     9061 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     9121 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     9181 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     9241 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     9301 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     9361 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     9421 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     9481 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     9541 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     9601 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     9661 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     9721 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     9781 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     9841 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     9901 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     9961 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
    10021 gcacgtacta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10081 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10141 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    10201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    10261 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
    10321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10381 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10501 cggccaacgc gcggggagag gcggtttgc
//
