LOCUS       sequence-370769-       10356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MAC-tagged gene expression of human ERBB3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10356)
  AUTHORS   Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, 
            Varjosalo M
  TITLE     Physical and functional interactome atlas of human receptor tyrosine
            kinases.
  JOURNAL   EMBO Rep. 2022 Apr 5:e54041. doi: 10.15252/embr.202154041.
  PUBMED    35384245
REFERENCE   2  (bases 1 to 10356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep. 
            2022 Apr 5:e54041. doi: 10.15252/embr.202154041."
COMMENT     Sequence Label: MAC_C_ERBB3
FEATURES             Location/Qualifiers
     source          1..10356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1995..2014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2186..2565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2566..2769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2720..2740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2771..2789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2792..2810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2820..2844
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2987..3011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7044..7068)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7115..7138
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7175..7198
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7229..7255
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             7229..7255
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(7256..7603,7604..7606,7607..8215)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(8259..8276)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8265..8489
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8622..8641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8773..8820
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8828..9850
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8828..9850
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9980..10101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10017..10036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10071..10090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10150..10166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10150..10166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10163..10185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10174..10190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10198..10204,10205..10222,10223..10228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10243..10264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     1981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     2041 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     2101 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     2161 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     2221 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2281 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2341 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2401 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2461 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2521 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2581 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2641 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2701 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2761 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
     2821 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     2881 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
     2941 gagctcggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa gtttgtacaa
     3001 aaaagcaggc tccaccatga gggcgaacga cgctctgcag gtgctgggct tgcttttcag
     3061 cctggcccgg ggctccgagg tgggcaactc tcaggcagtg tgtcctggga ctctgaatgg
     3121 cctgagtgtg accggcgatg ctgagaacca ataccagaca ctgtacaagc tctacgagag
     3181 gtgtgaggtg gtgatgggga accttgagat tgtgctcacg ggacacaatg ccgacctctc
     3241 cttcctgcag tggattcgag aagtgacagg ctatgtcctc gtggccatga atgaattctc
     3301 tactctacca ttgcccaacc tccgcgtggt gcgagggacc caggtctacg atgggaagtt
     3361 tgccatcttc gtcatgttga actataacac caactccagc cacgctctgc gccagctccg
     3421 cttgactcag ctcaccgaga ttctgtcagg gggtgtttat attgagaaga acgataagct
     3481 ttgtcacatg gacacaattg actggaggga catcgtgagg gaccgagatg ctgagatagt
     3541 ggtgaaggac aatggcagaa gctgtccccc ctgtcatgag gtttgcaagg ggcgatgctg
     3601 gggtcctgga tcagaagact gccagacatt gaccaagacc atctgtgctc ctcagtgtaa
     3661 tggtcactgc tttgggccca accccaacca gtgctgccat gatgagtgtg ccgggggctg
     3721 ctcaggccct caggacacag actgctttgc ctgccggcac ttcaatgaca gtggagcctg
     3781 tgtacctcgc tgtccacagc ctcttgtcta caacaagcta actttccagc tggaacccaa
     3841 tccccacacc aagtatcagt atggaggagt ttgtgtagcc agctgtcccc ataactttgt
     3901 ggtggatcaa acatcctgtg tcagggcctg tcctcctgac aagatggaag tagataaaaa
     3961 tgggctcaag atgtgtgagc cttgtggggg actatgtccc aaagcctgtg agggaacagg
     4021 ctctgggagc cgcttccaga ctgtggactc gagcaacatt gatggatttg tgaactgcac
     4081 caagatcctg ggcaacctgg actttctgat caccggcctc aatggagacc cctggcacaa
     4141 gatccctgcc ctggacccag agaagctcaa tgtcttccgg acagtacggg agatcacagg
     4201 ttacctgaac atccagtcct ggccgcccca catgcacaac ttcagtgttt tttccaattt
     4261 gacaaccatt ggaggcagaa gcctctacaa ccggggcttc tcattgttga tcatgaagaa
     4321 cttgaatgtc acatctctgg gcttccgatc cctgaaggaa attagtgctg ggcgtatcta
     4381 tataagtgcc aataggcagc tctgctacca ccactctttg aactggacca aggtgcttcg
     4441 ggggcctacg gaagagcgac tagacatcaa gcataatcgg ccgcgcagag actgcgtggc
     4501 agagggcaaa gtgtgtgacc cactgtgctc ctctggggga tgctggggcc caggccctgg
     4561 tcagtgcttg tcctgtcgaa attatagccg aggaggtgtc tgtgtgaccc actgcaactt
     4621 tctgaatggg gagcctcgag aatttgccca tgaggccgaa tgcttctcct gccacccgga
     4681 atgccaaccc atggagggca ctgccacatg caatggctcg ggctctgata cttgtgctca
     4741 atgtgcccat tttcgagatg ggccccactg tgtgagcagc tgcccccatg gagtcctagg
     4801 tgccaagggc ccaatctaca agtacccaga tgttcagaat gaatgtcggc cctgccatga
     4861 gaactgcacc caggggtgta aaggaccaga gcttcaagac tgtttaggac aaacactggt
     4921 gctgatcggc aaaacccatc tgacaatggc tttgacagtg atagcaggat tggtagtgat
     4981 tttcatgatg ctgggcggca cttttctcta ctggcgtggg cgccggattc agaataaaag
     5041 ggctatgagg cgatacttgg aacggggtga gagcatagag cctctggacc ccagtgagaa
     5101 ggctaacaaa gtcttggcca gaatcttcaa agagacagag ctaaggaagc ttaaagtgct
     5161 tggctcgggt gtctttggaa ctgtgcacaa aggagtgtgg atccctgagg gtgaatcaat
     5221 caagattcca gtctgcatta aagtcattga ggacaagagt ggacggcaga gttttcaagc
     5281 tgtgacagat catatgctgg ccattggcag cctggaccat gcccacattg taaggctgct
     5341 gggactatgc ccagggtcat ctctgcagct tgtcactcaa tatttgcctc tgggttctct
     5401 gctggatcat gtgagacaac accggggggc actggggcca cagctgctgc tcaactgggg
     5461 agtacaaatt gccaagggaa tgtactacct tgaggaacat ggtatggtgc atagaaacct
     5521 ggctgcccga aacgtgctac tcaagtcacc cagtcaggtt caggtggcag attttggtgt
     5581 ggctgacctg ctgcctcctg atgataagca gctgctatac agtgaggcca agactccaat
     5641 taagtggatg gcccttgaga gtatccactt tgggaaatac acacaccaga gtgatgtctg
     5701 gagctatggt gtgacagttt gggagttgat gaccttcggg gcagagccct atgcagggct
     5761 acgattggct gaagtaccag acctgctaga gaagggggag cggttggcac agccccagat
     5821 ctgcacaatt gatgtctaca tggtgatggt caagtgttgg atgattgatg agaacattcg
     5881 cccaaccttt aaagaactag ccaatgagtt caccaggatg gcccgagacc caccacggta
     5941 tctggtcata aagagagaga gtgggcctgg aatagcccct gggccagagc cccatggtct
     6001 gacaaacaag aagctagagg aagtagagct ggagccagaa ctagacctag acctagactt
     6061 ggaagcagag gaggacaacc tggcaaccac cacactgggc tccgccctca gcctaccagt
     6121 tggaacactt aatcggccac gtgggagcca gagcctttta agtccatcat ctggatacat
     6181 gcccatgaac cagggtaatc ttggggagtc ttgccaggag tctgcagttt ctgggagcag
     6241 tgaacggtgc ccccgtccag tctctctaca cccaatgcca cggggatgcc tggcatcaga
     6301 gtcatcagag gggcatgtaa caggctctga ggctgagctc caggagaaag tgtcaatgtg
     6361 tagaagccgg agcaggagcc ggagcccacg gccacgcgga gatagcgcct accattccca
     6421 gcgccacagt ctgctgactc ctgttacccc actctcccca cccgggttag aggaagagga
     6481 tgtcaacggt tatgtcatgc cagatacaca cctcaaaggt actccctcct cccgggaagg
     6541 caccctttct tcagtgggtc tcagttctgt cctgggtact gaagaagaag atgaagatga
     6601 ggagtatgaa tacatgaacc ggaggagaag gcacagtcca cctcatcccc ctaggccaag
     6661 ttcccttgag gagctgggtt atgagtacat ggatgtgggg tcagacctca gtgcctctct
     6721 gggcagcaca cagagttgcc cactccaccc tgtacccatc atgcccactg caggcacaac
     6781 tccagatgaa gactatgaat atatgaatcg gcaacgagat ggaggtggtc ctgggggtga
     6841 ttatgcagcc atgggggcct gcccagcatc tgagcaaggg tatgaagaga tgagagcttt
     6901 tcaggggcct ggacatcagg ccccccatgt ccattatgcc cgcctaaaaa ctctacgtag
     6961 cttagaggct acagactctg cctttgataa ccctgattac tggcatagca ggcttttccc
     7021 caaggctaat gcccagagaa cggacccagc tttcttgtac aaagtggttg atatcaaggc
     7081 cgccgacatc accagcctgt acaagaaagt ggggtggagc catccccagt tcgagaaggg
     7141 cggaggatct ggcggcggaa gcggcggagg aagctggtcc caccctcagt ttgagaaagg
     7201 caccgagctg ggcagcacca tggccagcta cccctacgac gtgcccgact acgccaagga
     7261 caacaccgtg cccctgaagc tgatcgccct gctggccaac ggcgagttcc actctggcga
     7321 gcagctgggc gagacactgg gcatgagcag agccgccatc aacaagcaca tccagaccct
     7381 gcgggactgg ggcgtggacg tgttcacagt gcccggcaag ggctacagcc tgcccgagcc
     7441 catccagctg ctgaacgcca agcagatcct gggccagctg gatggcggat ctgtggccgt
     7501 gctgcccgtg atcgacagca ccaaccagta tctgctcgac cggatcggcg agctgaagtc
     7561 cggcgacgcc tgtatcgccg agtaccagca ggccggcaga ggcggcaggg gcagaaagtg
     7621 gttcagcccc ttcggcgcca acctgtacct gagcatgttc tggagactgg aacagggccc
     7681 agccgccgct attggcctga gcctggtcat cggcatcgtg atggccgagg tgctgcggaa
     7741 gctgggcgcc gacaaagtgc gcgtgaagtg gcccaacgac ctgtatctgc aggaccggaa
     7801 gctggccggc atcctggtgg aactgaccgg caaaacaggc gacgccgccc agatcgtgat
     7861 cggcgctggc atcaacatgg ccatgcggcg ggtggaagag agcgtggtca accagggctg
     7921 gatcaccctg caggaagccg gcatcaacct ggaccggaac accctggccg ccatgctgat
     7981 cagagagctg agagccgccc tggaactgtt cgagcaggaa ggcctggccc cctacctgag
     8041 cagatgggag aagctggaca acttcatcaa ccggcccgtg aaactgatca tcggcgacaa
     8101 agagatcttc ggcatcagcc ggggcatcga caagcagggg gccctgctcc tggaacagga
     8161 cggcatcatc aagccctgga tgggcggcga gatcagcctg cggagcgccg agaagtgatg
     8221 actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8281 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8341 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8401 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8461 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8521 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8581 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8641 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8701 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     8761 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     8821 cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     8881 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     8941 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     9001 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     9061 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     9121 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     9181 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     9241 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     9301 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     9361 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     9421 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     9481 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     9541 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     9601 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     9661 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     9721 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     9781 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     9841 aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9901 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9961 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    10021 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10081 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    10141 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10321 ttaatgaatc ggccaacgcg cggggagagg cggttt
//
