LOCUS       sequence-370777-       13556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Worm Cas9 expression vector, Peft-3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 13556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS485
FEATURES             Location/Qualifiers
     source          1..13556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        402..1001
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     protein_bind    1010..1034
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10990..11313
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11335..11351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11335..11351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11348..11370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11359..11375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11383..11389,11390..11407,11408..11413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11428..11449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11566..11583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11737..12325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11817..11836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12496..13287,13288..13356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13119..13138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13357..13461)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13529..13547
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgcacctttg gtcttttatt
      421 gtcaacttcc attggttctt ccattgtttc tgttaaatta atgaattttt cataaaataa
      481 agacattata caatataaaa atgaagaatt tattgaaaat aaactgccag agagaaaaag
      541 tatgcaacac tcccgccgag agtgtttgaa atggtgtacg gtacattttc gtgctaggag
      601 ttagatgtgc aggcagcaac gagaggggga gagatttttt tgggccttgt gaaattaacg
      661 tgagttttct ggtcatctga ctaatcatgt tggttttttg ttggtttatt ttgtttttat
      721 ctttgttttt atccagatta ggaaatttaa attttatgaa tttataatga ggtcaaacat
      781 tcagtcccag cgtttttcct gttctcactg tttagtcgaa tttttatttt aggctttcaa
      841 caaatgttct aactgtctta tttgtgacct cactttttat atttttttaa tttttaaaaa
      901 tattagaagt ttctaggata attttttcga cttttattct ctctaccgtc cgcactcttc
      961 ttacttttaa attaaattgt ttttttttca gttgggaaac actttgctca caagtttgta
     1021 caaaaaagca ggcttaatgg acaagaagta ctccatcgga ctcgacatcg gaaccaactc
     1081 cgtcggatgg gccgtcatca ccgacgagta caaggtccca tccaagaagt tcaaggtcct
     1141 cggaaacacc gaccgtcact ccatcaagaa gaacctcatc ggagccctcc tcttcgactc
     1201 cggagagacc gccgaggcca cccgtctcaa gcgtaccgcc cgtcgtcgtt acacccgtcg
     1261 taagaaccgt atctgctacc tccaggagat cttctccaac gagatggcca aggtcgacga
     1321 ctccttcttc caccgtctcg aggagtcctt cctcgtcgag gaggacaaga agcacgagcg
     1381 tcacccaatc ttcggaaaca tcgtcgacga ggtcgcctac cacgagaagt acccaaccat
     1441 ctaccacctc cgtaagaagc tcgtcgactc caccgacaag gccgacctcc gtctcatcta
     1501 cctcgccctc gcccacatga tcaagttccg tggacacttc ctcatcgagg gagacctcaa
     1561 cccagacaac tccgacgtcg acaagctctt catccagctc gtccagacct acaaccagct
     1621 cttcgaggag aacccaatca acgcctccgg agtcgacgcc aaggccatcc tctccgcccg
     1681 tctctccaag tcccgtcgtc tcgagaacct catcgcccag ctcccaggag agaagaagaa
     1741 cggactcttc ggaaacctca tcgccctctc cctcggactc accccaaact tcaagtccaa
     1801 cttcgacctc gccgaggacg ccaagctcca gctctccaag gacacctacg acgacgacct
     1861 cgacaacctc ctcgcccaga tcggagacca gtacgccgac ctcttcctcg ccgccaagaa
     1921 cctctccgac gccatcctcc tctccgacat cctccgtgta aggagttgaa cggctgaaaa
     1981 atcgatattt tgagcgaaaa aagccggaaa aatggatttt atcggataaa atttgatttt
     2041 ttgagctgag aaatgccttt atagacgaat ttccttgtca aattgttaaa aactgcaaaa
     2101 tttgccaaaa aaaaagcatt ttttatattt ttttttacaa aaaattccac aaaaatggca
     2161 aaatttgact aaaaatgcca attttgtcgt tttctccgtg ccacagcggc cgaaaatcga
     2221 tttttaagcg attttcgggt gaaaaagtgt aaaaaccgac tgaaaatcca gctgaaaacg
     2281 acaaaaacgg taagttttag ctaattttca cctttttttc gatatttttt tactaaaaaa
     2341 aaaccgaaaa attgagtttt tttcaaattt ctgcaaaaat tctgcaattt tagcaatttc
     2401 catactattt tgacgccgaa aaagcttaaa aatatgattt ttagaggttt ttaatggaaa
     2461 attcatggat ttttcaaatt tttccacgaa aatcattgaa aaatttgaaa aaattgattt
     2521 cctattcaaa gttctagcta aaaactgcaa ttttagcaat ttccatacta ttttagtact
     2581 gaaaaagcct aaaaatgtga ttttcagagg atttcaatgg aaaattcatg gatttttcca
     2641 atttttcaat gaaaaccatt gaaaaaccct ggaaaattga gtttttcttc aaagtttagg
     2701 aaatttatga gaatttttga ataaaaaatt tttaaatttg aaaaaaattc tcataaattt
     2761 cgtaaacttt taataaaaac tcaatttttc cgattttttt cgatgatttt cactaaaaaa
     2821 ttggaaaaat tcatcaattt cctattgaaa acctctaaaa attatatttt ttttgctttt
     2881 ccgacgtcaa aatagtatgg aaaatgctaa aattgcagaa tttttccaga aatttgaaga
     2941 aaaaactcaa ttttttccgt tttttcaatg attttcatga aaaaattgga aaattttgta
     3001 aattttccat tgaaaacctc tgaaaatttg gaaattttcg aatttaaatg tttaaaaaaa
     3061 attgaatatt ttgcgagttt ttgaattttt tttgagaatt tttgaatttt tttcacccaa
     3121 aattttttca atttttcagg tcaacaccga gatcaccaag gccccactct ccgcctccat
     3181 gatcaagcgt tacgacgagc accaccagga cctcaccctc ctcaaggccc tcgtccgtca
     3241 gcagctccca gagaagtaca aggagatctt cttcgaccag tccaagaacg gatacgccgg
     3301 atacatcgac ggaggagcct cccaggagga gttctacaag ttcatcaagc caatcctcga
     3361 gaagatggac ggaaccgagg agctcctcgt caagctcaac cgtgaggacc tcctccgtaa
     3421 gcagcgtacc ttcgacaacg gatccatccc acaccagatc cacctcggag agctccacgc
     3481 catcctccgt cgtcaggagg acttctaccc attcctcaag gacaaccgtg agaagatcga
     3541 gaagatcctc accttccgta tcccatacta cgtcggacca ctcgcccgtg gaaactcccg
     3601 tttcgcctgg atgacccgta agtccgagga gaccatcacc ccatggaact tcgaggaggt
     3661 cgtcgacaag ggagcctccg cccagtcctt catcgagcgt atgaccaact tcgacaagaa
     3721 cctcccaaac gagaaggtcc tcccaaagca ctccctcctc tacgagtact tcaccgtcta
     3781 caacgagctc accaaggtca agtacgtcac cgagggaatg cgtaagccag ccttcctctc
     3841 cggagagcag aagaaggcca tcgtcgacct cctcttcaag accaaccgta aggtcaccgt
     3901 caagcagctc aaggaggact acttcaagaa gatcgagtgc ttcgactccg tcgagatctc
     3961 cggagtcgag gaccgtttca acgcctccct cggaacctac cacgacctcc tcaagatcat
     4021 caaggacaag gacttcctcg acaacgagga gaacgaggac atcctcgagg acatcgtcct
     4081 caccctcacc ctcttcgagg accgtgagat gatcgaggtg ctgaagctcg aaaatttgga
     4141 gcttttacac gagatttttg aagaaaaagc ctgaaaatcg agagattttg agctgtggaa
     4201 ttcgttgaaa ttacataatt cgcgcccgta attctttaaa aaaacgctga aatttgcaga
     4261 ttttgccaga aagttgaaga aaaatctact tttccggcat tctctattat tttcatggaa
     4321 aattagaaaa atccatgaat aaacagtgaa aattgctgat tttacctgaa attttgaaaa
     4381 aaaaaaaaat ctaaaatcaa ggttttaagc ctaaaaactg cagatttcag ccaaaattgt
     4441 gaaaaaagct gattttttgc tattttgagc ttttccatgg ggtggaaatt gccaaaaatt
     4501 acggaacatt cacacaaatt tgctgaataa tccaattttc cagtgttttc tagagtttcc
     4561 gtagaaattt tgaaaaaacc cagtaaattt tcaatcggaa aacctctaaa aacatcgttt
     4621 caggcctata aatagtaaaa attaccgatt ttattcgaaa atttaaggaa aagtcgtgaa
     4681 aaacgagatt ttaagcctca aaactgcaga ttctatctaa aattaaaaaa aaaaaacata
     4741 aatatccgaa tttttggagg aaaagcctga aaatcgatat tttaagcctc aaaactgaag
     4801 atttcagcca aaaaaaaaaa cagatttttc gctattttat gtgccaaaaa accacattcc
     4861 gagcttttcc agagtggaaa ttgccaaaaa ttacagaatt ttcccagaaa ttagcaaaaa
     4921 aaaaacccat ttttcaggcg ttttcaacga ttttcatagc aaatttgaaa aatccatgaa
     4981 ttttttaatt atagaaaacc ctctgaaaat tgcagtttta agctattcca gcattcaaat
     5041 agtatggaaa ttgctaaaaa ttgaagaatt ttcccaaaaa tttgatgaaa acttattttt
     5101 ctggcgtttt cgatgatttt catggaattt cgagaaaatt aatgaatttt caattaaaaa
     5161 acgtctgcaa aatcacattt tttaaagctt tttcagcaac aaaatagtat ggaaattgct
     5221 ttttaaaaaa gcggaatttt tggaaaaaag gttaaatttt cagtcagttt ttgcactttt
     5281 tgactgaaaa ttcactgaaa ttagggaaaa attccggaaa aatgattttt tcgaattgta
     5341 gggcaaattt tgcaatttca agcaatttcc ataatatttt aatgctggaa aagctcaaat
     5401 ttacgtttta atgggatttt agtgaaaaat tcatggattt tccgaaaaat ttgcaggaaa
     5461 atcatagtaa agacctcaaa aaatcaataa aaaatttaaa aaattggaca atcacatttc
     5521 tgcgtaaaat ctgcactttt tcagtaaaaa ataccgattt tccgtgaaaa ttctcgattt
     5581 ttaatcgaaa atctcgaaat ttttacaaat ttcagcacca aaatcgttga aaattctgaa
     5641 aatttgaatt ttttttcctt gttttttgag taaaatttgc actttttcag taaagaatac
     5701 cgattttccg tgaaaattct cggtttttaa tcgaaaatct cgaaattttt acaaatttca
     5761 gctccgaaat agctgaaaat tggacaaaaa tttcaatttt tgccctgttt tttgagtaaa
     5821 atctgcactt tttagttgaa aaaaaacacg gattttgtag gaaaatttgg gaaaaatctc
     5881 caaattttca caattttcca gtaaaaacag caatttttaa aacggttttt aatcaaaaat
     5941 ctttagaatt ttccaaattt cagcaccaaa aaagctaaaa atcgctgaaa ttttcgacaa
     6001 attccaaatt tccaggagcg tctcaagacc tacgcccacc tcttcgacga caaggtcatg
     6061 aagcagctca agcgtcgtcg ttacaccgga tggggacgtc tctcccgtaa gctcatcaac
     6121 ggaatccgtg acaagcagtc cggaaagacc atcctcgact tcctcaagtc cgacggattc
     6181 gccaaccgta acgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
     6241 cagttcatgc agctcatcca cgacgactcc ctcaccttca aggaggacat ccagaaggcc
     6301 caggtctccg gacagggaga ctccctccac gagcacatcg ccaacctcgc cggatcccca
     6361 gccatcaaga agggaatcct ccagaccgtc aaggtcgtcg acgagctcgt caaggtcatg
     6421 ggacgtcaca agccagagaa catcgtcatc gagatggccc gtgagaacca gaccacccag
     6481 aagggacaga agaactcccg tgagcgtatg aagcgtatcg aggagggaat caaggagctc
     6541 ggatcccaga tcctcaagga gcacccagtc gagaacaccc agctccagaa cgagaagctc
     6601 tacctctact acctccagaa cggacgtgac atgtacgtcg accaggagct cgacatcaac
     6661 cgtctctccg actacgacgt cgaccacatc gtcccacagt ccttcctcaa ggacgactcc
     6721 atcgacaaca aggtcctcac ccgttccgac aagaaccgtg gaaagtccga caacgtccca
     6781 tccgaggagg tcgtcaagaa gatgaagaac tactggcgtc agctcctcaa cgccaagctc
     6841 atcacccagc gtaagttcga caacctcacc aaggccgagc gtggaggact ctccgagctc
     6901 gacaaggccg gattcatcaa gcgtcagctc gtcgagaccc gtcagatcac caagcacgtc
     6961 gcccagatcc tcgactcccg tatgaacacc aagtacgacg agaacgacaa gctcatccgt
     7021 ggttggtttt tccgagagaa aaacgctgaa aaatgccgaa aattttgaat ttgcaggctt
     7081 tttaggtgca aaagtacgga aaaatcggaa aaaaatcagg aaaacatggg gaaaatccaa
     7141 ttttccagaa aaaatgtttg aaaaaattga aaacttttgt ttttttagta ataaagtttt
     7201 aaaaaatggc aaaaatagct gaaaacagcg aaaaaaaaaa atgttttttt caggaaaaaa
     7261 tttgcaaaaa aatgaagagc aacaataatt cccttttttt tctgtgtttt tttcggaaaa
     7321 attgagatcg aaaacgaaaa acagcgaaaa ttcccaattt tcccagtttt taaaacattt
     7381 ttttgtaaaa atttggaaaa aacgagtttt ttcagaaaaa aatgtgaaaa aaaaggtaaa
     7441 ttaccaattt atgctttttt ttcgatcgag taaaaaatcg atttttccaa gatttttcgc
     7501 gaaaaattca aaaaaaaaga acgaaaattc tcgattttct actttttttc gaaaaaaaaa
     7561 ggaaattata gatttttcca agatttttcg cgaaaaaatt cgaaaaaaat gtttttttcg
     7621 aaaatttcag attttctaat tttttcagaa gaattcgaaa aaaaaaatca aaaaattcgc
     7681 aatttttaga tttttttaaa atctcaattt tctataatta ttttcctaaa aaaatgtaat
     7741 taaaaaaccg ctaaaaacgg taaattccaa atttttgcat tttttcaaaa aaaatttgaa
     7801 aaaaaaaatc agaaaattct caaatctatt cccttttttt aaaatttttc ccaaaccaaa
     7861 aaaaaaaaac ggtaaatttt caaatttagc atttttccaa aattaaacat aaaaaaaaac
     7921 gcaaatttcc aaatttcgtt tttttttcaa aaaagttgaa aaatccagaa aataccctct
     7981 tgtccatgaa aaaatacgaa aattttcaat aaaaaatcaa tttcttctta aaaaattaat
     8041 tctcgatttt tccatgaaaa aaaatcaaat attaattaaa tttgaaaaaa aaaagaattt
     8101 atctaatagt ttcgtttttt ttcagaggaa aaatttgaaa aaaaaaacag taaattctct
     8161 ttctttttga aaaaaaaaat tatcgattct tccaagtttt ttcccgaaaa aatcgacaaa
     8221 aaaacgagaa tttccgattt tctatttttt ttttcaaaaa aatgtgaaaa aaaaaattaa
     8281 aaaattccaa ttttgcattt ttttttcaaa aaaaatttaa aaacaccgta taattcccga
     8341 tttttccatg aaaaaatacg aaaaattcca ataaaaatca actattttaa aaatttgccg
     8401 ttttttcaga ggtcaaggtc atcaccctca agtccaagct cgtctccgac ttccgtaagg
     8461 acttccagtt ctacaaggtc cgtgagatca acaactacca ccacgcccac gacgcctacc
     8521 tcaacgccgt cgtcggaacc gccctcatca agaagtaccc aaagctcgag tccgagttcg
     8581 tctacggaga ctacaaggtc tacgacgtcc gtaagatgat cgccaagtcc gagcaggaga
     8641 tcggaaaggc caccgccaag tacttcttct actccaacat catgaacttc ttcaagaccg
     8701 agatcaccct cgccaacgga gagatccgta agcgtccact catcgagacc aacggagaga
     8761 ccggagagat cgtctgggac aagggacgtg acttcgccac cgtccgtaag gtcctctcca
     8821 tgccacaggt caacatcgtc aagaagaccg aggtccagac cggaggattc tccaaggagt
     8881 ccatcctccc aaagcgtaac tccgacaagc tcatcgcccg taagaaggac tgggacccaa
     8941 agaagtacgg aggattcgac tccccaaccg tcgcctactc cgtcctcgtc gtcgccaagg
     9001 tcgagaaggg aaagtccaag aagctcaagt ccgtcaagga gctcctcgga atcaccatca
     9061 tggagcgttc ctccttcgag aagaacccaa tcgacttcct cgaggccaag ggatacaagg
     9121 aggtcaagaa ggtgcttaca attttctgga gaaaaatatt ttaaaaaata acgaaaatgg
     9181 accgaatttt tggagaaaat tttaaaaaaa acgacatttt tcccaaaaaa ttgaaaaatt
     9241 tccgaaaaaa cgttcaaaat tttcaaaaaa ggagcatttt tcgaaaaaaa aaacgataat
     9301 acggaaattt tttgattttc cagttttttt ctcaaagaaa atcggaaaaa ggtttttttt
     9361 ctttaaaaac gtccaaaatt tttcgaaaaa agagaaaatt ttcgattttc cgattttttt
     9421 cagaaaaaaa ttgaaaaaaa aagaaatttt ccgattttct cagagaaaga cgaaaagacg
     9481 atatttttcg gaaaaaagtt gaaaaatcaa aaaaaaaacc gaaaaacggc cgaaattttc
     9541 taaaaattgg aaatattgtc gatttttcga aaaaaaagag tcaaaagttt tcaaaaaaat
     9601 atattttttc gattttccgt tttttttttt cagtaaacat tgaaacaaac gaaatttttc
     9661 caaaaaaatc caattatttt cccaaatatc gaaatatttt tgattttccg tttttttcag
     9721 aaaaaagtta aaaaaaaaag ggaaaattaa gattttcctg attttttttg agaaaaaatc
     9781 gataaaaatt gaatattctg gaaaaaacct ttaaaaaatt cgaattaaat cgaaaaacgt
     9841 ccaaaatttt ctaaaaaatt gaaatttttt ttgaaaaaaa acggaaaaat ttagatttcc
     9901 cgactttttt caatgaaaaa tcgaattttc gttgactttt cattaaaaaa tccaaacgca
     9961 tttttttcca ctaaaatctc gaaaaagtgt catattgcac catattgttt ccacattcat
    10021 attattttga gctaaatcag gtgattttct gtattttgat actaatttct atttttagaa
    10081 aaaatgtggg aacaatatgg tgcaatatga cattttttcg agattttagt ggagaaaaaa
    10141 tgcgtttgga aacgaatttt ataataaaaa gtcaacgaaa attcgatttt tcatggaaaa
    10201 atagggaaat cgaaaacaaa tttgcttttt tttcaaaaaa aaaaacggaa attcgaaatt
    10261 tttccgttct ttcaaaaaaa aaatcgattt ttttggaaat tttttttttc aaatttttaa
    10321 aaatttccag gacctcatca tcaagctccc aaagtactcc ctcttcgagc tcgagaacgg
    10381 acgtaagcgt atgctcgcct ccgccggaga gctccagaag ggaaacgagc tcgccctccc
    10441 atccaagtac gtcaacttcc tctacctcgc ctcccactac gagaagctca agggatcccc
    10501 agaggacaac gagcagaagc agctcttcgt cgagcagcac aagcactacc tcgacgagat
    10561 catcgagcag atctccgagt tctccaagcg tgtcatcctc gccgacgcca acctcgacaa
    10621 ggtcctctcc gcctacaaca agcaccgtga caagccaatc cgtgagcagg ccgagaacat
    10681 catccacctc ttcaccctca ccaacctcgg agccccagcc gccttcaagt acttcgacac
    10741 caccatcgac cgtaagcgtt acacctccac caaggaggtc ctcgacgcca ccctcatcca
    10801 ccagtccatc accggactct acgagacccg tatcgacctc tcccagctcg gaggagactc
    10861 tggaggatct ggaggaactg gaggatcttc cgccggcggg agctctatga gccgtagacg
    10921 aaaagcgaat ccgacaaaac tgagtgaaaa cgcgaagaag cttgccaagg aagttgaaaa
    10981 ttaagataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt
    11041 ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt
    11101 ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt
    11161 ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt
    11221 tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag
    11281 aggaaacttt attgtgccgc caagaaaaaa gtctcaaagc ttggcgtaat catggtcata
    11341 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    11401 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    11461 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    11521 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    11581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    11641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    11701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    11761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    11821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    11881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    11941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    12001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    12061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    12121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    12181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    12241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    12301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    12361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    12421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    12481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    12541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    12601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    12661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    12721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    12781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    12841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    12901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    12961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    13021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    13081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    13141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    13201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    13261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    13321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    13381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    13441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
    13501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
