LOCUS       sequence-370785-       16288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed 
            Cbr-Unc-119.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16288)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 16288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS714
FEATURES             Location/Qualifiers
     source          1..16288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    346..379
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             2338..2349
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    2498..2531
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     2554..2570
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2554..2570
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2590..2610
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3114..3138
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3150..3170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(13251..13275)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      13280..13603
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(13607..13627)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(13635..13652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13635..13651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(13657..13668)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     14338..14360
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(14398..14416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        14484..14588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14589..14657,14658..15449)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14807..14826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15620..16208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16109..16128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgcac cgtacgtctc
       61 gaggaattct atcaggtgta caagtatgaa atgtcgtttc gaacatatag atgtctcgca
      121 aacgtaaata tttatcgatt gtcataaaac tttgaccttg tgaagtgtca accttgactg
      181 tcgaaccacc atagtttggc gcgaattgag cgtcataatt gtttactctc agtgcagtca
      241 acatgtcgag tttcgtgccg aataaagagc aaacgcggac agtattaatt ttctgttttc
      301 atttgaagaa aacagctgcg gcagtttaaa catgatttta ctaacataac ttcgtataaa
      361 gtatcctata cgaagttatg acattctcta atgaaaaaat ctttcagttg aaattgaaaa
      421 tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg tttttagcga
      481 gtgtgacagg acagcgaaaa aatatagaaa caagggggga actgaaaagc ttaggaatgc
      541 attgaacatg agaaggggaa agggaaggaa caaactagac aggaattatt ggaatttaat
      601 cacatttgga gttttttttc tattcgacag aataattatc cagaacattt ttgtattaaa
      661 tatttatgca tcatatgagt agtcggcttt gttgtgcatg acgagtttgt tatcgacgaa
      721 atagaagctg tcagaacgag tctcgtttgg attgttgatc atgtcgtcca ctgaaaaaga
      781 gattagtctt ttgaattgta ctttttagaa taatgactca ctgagttgtt gagagagttg
      841 agggaactca tagatatgtt cacagttgtt tcgtgaattc ggaatacaga atccgaattc
      901 aaagtcaaaa cacttcagaa ggcgatcttt gaagaagtga cgttcgatca ttcggaaatg
      961 atggattggg atgtctccta ctttgaattc cacagttgct ccgaccgttt tgagtttcaa
     1021 aaagtttgga gcaaatctat aacgcacgta tcttgccgac tcctgtggcg actcatcatt
     1081 ctcctgatcg ttctccggtt tggcgatctc gaaaagcact tgctcagtgt ccagatcacg
     1141 gatttggaac ttggtgaact cgatgttata gatgttcgca gatggggagc ataagaatcc
     1201 taaatttatg ttttaaactg aaatccaaag ggagcaagat accttgagtg attcccggaa
     1261 gtgctaaaac gtcgttcgga gtgatttgag ctttcttcgc aagctccgat tccgttgtga
     1321 ttccttgttc ggtgcttggt ggtggccgtg gcatctggaa atatggaaaa gttcaacaaa
     1381 aagaaaagag aaaagaatga aatcggatat caagagttag ttgagcggtt tctctagttt
     1441 tctgagtctc acctgcgacg ggaaggtcgc cgagccgggt ggaatcgatc gttgttgctc
     1501 ggctttcata tcggtttggt tggaagcggc tgaaaacgga aagaagtgga agaaggaaaa
     1561 gagtgtggtg tgacaggaaa atggtaatta gagggtgcca aataaccagc tatattttgt
     1621 ttttttttga aaacattttt aaaaagaaaa atacgataat gatatcagat ggatttccgg
     1681 aaaactggta tgaaaaattt caaccttttt gagtacatgt aatcaaaata cactttgtaa
     1741 attatcattt ttattgaaac tccaccattt ttctatttat aacgctaata atttgaaaaa
     1801 gaaacctatt gcgaaccgcg gggtgaatcc caaaaacgaa tgcgttttgg tggagtgatt
     1861 gattcgaatc gaagaagaaa aagaagaaga cgtggaatag agagctcact cttaaccgag
     1921 cagcacacac cgacagaaaa aaaaatgaaa tgaatgaggg tcttcttctt cttcttcttc
     1981 gaatgattga cagaaatggg aaaaagagga agattgagaa gggaaaaagg aaggagaaaa
     2041 gaagcagaag aagacgtcag agaggagagg aacgagcgga aaagcagcgg gcgcaagtca
     2101 tagaagtagc agagctgggg agaagaagac actatccaag aaaggaatga cgagagagta
     2161 tgcaaagggg tatagggtgc agacagaata ggaacagaat aacagatgat gagccaagaa
     2221 gagttgaaaa gggcgatgaa tttgtcatgt aacttaattt gggtcaattt gagcatgatg
     2281 aattgaaatc atcccttgtt gggagttaat aaccggtttg ttatcagaaa ccctgtaata
     2341 gaagggcgcc ctaactttga gccaattcat cccggtttct gtcaaatata tcaaaaagtg
     2401 gtcaactgac aaattgtttt tgatattata ataaacattt tatccgttaa caattttcga
     2461 atacttttta caaggacttg gataaattgg ctcaaagata acttcgtata aagtatccta
     2521 tacgaagtta ttaactaatc tgatttaaat tttcaggaaa cagctatgac catgattacg
     2581 ccaagctatc aactttgtat agaaaagttg ggttttcaca acggcaaaat atcagttttt
     2641 aaaaaattaa accataaaac aaataatata acccaatttt tacatcaaac cacaagaaaa
     2701 aaatacattt gggcccacgg ataaagaaat taaaaaaata cattttttaa aggcgcaccg
     2761 aattaaaatt catttgggtc ttaccgcgta taccgtactc cgtttgtttg atcatttttg
     2821 tcagcgctgg cggttgtttt ttcatttcat ttctgcttca aagacgtttt ctcgaataat
     2881 ttttcgttta ttctcttttt taaaattaat ttctagccgt aaatgttata aattcaccca
     2941 tttaacgcaa atttcatggt aatctcatgg aaaaatgcag tttctttgtt aaagaaagct
     3001 taaatagcaa aaattccccg actttcccca aaatcctgct cgattttccg ttttctcatt
     3061 gtattctctc ttaattaatt ttatcgataa tcaattgaat gtttcagaca gagacaagtt
     3121 tgtacaaaaa agcaggctcg atgactgctc caaagaagaa gcgtaaggta ccggtagaaa
     3181 aaatggacaa gaagtactcc atcggactcg acatcggaac caactccgtc ggatgggccg
     3241 tcatcaccga cgagtacaag gtcccatcca agaagttcaa ggtcctcgga aacaccgacc
     3301 gtcactccat caagaagaac ctcatcggag ccctcctctt cgactccgga gagaccgccg
     3361 aggccacccg tctcaagcgt accgcccgtc gtcgttacac ccgtcgtaag aaccgtatct
     3421 gctacctcca ggagatcttc tccaacgaga tggccaaggt cgacgactcc ttcttccacc
     3481 gtctcgagga gtccttcctc gtcgaggagg acaagaagca cgagcgtcac ccaatcttcg
     3541 gaaacatcgt cgacgaggtc gcctaccacg agaagtaccc aaccatctac cacctccgta
     3601 agaagctcgt cgactccacc gacaaggccg acctccgtct catctacctc gccctcgccc
     3661 acatgatcaa gttccgtgga cacttcctca tcgagggaga cctcaaccca gacaactccg
     3721 acgtcgacaa gctcttcatc cagctcgtcc agacctacaa ccagctcttc gaggagaacc
     3781 caatcaacgc ctccggagtc gacgccaagg ccatcctctc cgcccgtctc tccaagtccc
     3841 gtcgtctcga gaacctcatc gcccagctcc caggagagaa gaagaacgga ctcttcggaa
     3901 acctcatcgc cctctccctc ggactcaccc caaacttcaa gtccaacttc gacctcgccg
     3961 aggacgccaa gctccagctc tccaaggaca cctacgacga cgacctcgac aacctcctcg
     4021 cccagatcgg agaccagtac gccgacctct tcctcgccgc caagaacctc tccgacgcca
     4081 tcctcctctc cgacatcctc cgtgtaagga gttgaacggc tgaaaaatcg atattttgag
     4141 cgaaaaaagc cggaaaaatg gattttatcg gataaaattt gattttttga gctgagaaat
     4201 gcctttatag acgaatttcc ttgtcaaatt gttaaaaact gcaaaatttg ccaaaaaaaa
     4261 agcatttttt atattttttt ttacaaaaaa ttccacaaaa atggcaaaat ttgactaaaa
     4321 atgccaattt tgtcgttttc tccgtgccac agcggccgaa aatcgatttt taagcgattt
     4381 tcgggtgaaa aagtgtaaaa accgactgaa aatccagctg aaaacgacaa aaacggtaag
     4441 ttttagctaa ttttcacctt tttttcgata tttttttact aaaaaaaaac cgaaaaattg
     4501 agtttttttc aaatttctgc aaaaattctg caattttagc aatttccata ctattttgac
     4561 gccgaaaaag cttaaaaata tgatttttag aggtttttaa tggaaaattc atggattttt
     4621 caaatttttc cacgaaaatc attgaaaaat ttgaaaaaat tgatttccta ttcaaagttc
     4681 tagctaaaaa ctgcaatttt agcaatttcc atactatttt agtactgaaa aagcctaaaa
     4741 atgtgatttt cagaggattt caatggaaaa ttcatggatt tttccaattt ttcaatgaaa
     4801 accattgaaa aaccctggaa aattgagttt ttcttcaaag tttaggaaat ttatgagaat
     4861 ttttgaataa aaaattttta aatttgaaaa aaattgaaat tttaattcta aattttaaag
     4921 aaagttgatt ttttaataat tttttttttg aataaaaaaa aatcaacttt ctttaaaatt
     4981 tagaattaaa atttcaattt ttttattcaa aaattctcat aaatttcgta aacttttaat
     5041 aaaaactcaa tttttccgat ttttttcgat gattttcact aaaaaattgg aaaaattcat
     5101 caatttccta ttgaaaacct ctaaaaatta tatttttttt gcttttccga cgtcaaaata
     5161 gtatggaaaa tgctaaaatt gcagaatttt tccagaaatt tgaagaaaaa actcaatttt
     5221 ttccgttttt tcaatgattt tcatgaaaaa attggaaaat tttgtaaatt ttccattgaa
     5281 aacctctgaa aatttggaaa ttttcgaatt taaatgttta aaaaaaattg aatattttgc
     5341 gagtttttga attttttttg agaatttttg aatttttttc acccaaaatt ttttcaattt
     5401 ttcaggtcaa caccgagatc accaaggccc cactctccgc ctccatgatc aagcgttacg
     5461 acgagcacca ccaggacctc accctcctca aggccctcgt ccgtcagcag ctcccagaga
     5521 agtacaagga gatcttcttc gaccagtcca agaacggata cgccggatac atcgacggag
     5581 gagcctccca ggaggagttc tacaagttca tcaagccaat cctcgagaag atggacggaa
     5641 ccgaggagct cctcgtcaag ctcaaccgtg aggacctcct ccgtaagcag cgtaccttcg
     5701 acaacggatc catcccacac cagatccacc tcggagagct ccacgccatc ctccgtcgtc
     5761 aggaggactt ctacccattc ctcaaggaca accgtgagaa gatcgagaag atcctcacct
     5821 tccgtatccc atactacgtc ggaccactcg cccgtggaaa ctcccgtttc gcctggatga
     5881 cccgtaagtc cgaggagacc atcaccccat ggaacttcga ggaggtcgtc gacaagggag
     5941 cctccgccca gtccttcatc gagcgtatga ccaacttcga caagaacctc ccaaacgaga
     6001 aggtcctccc aaagcactcc ctcctctacg agtacttcac cgtctacaac gagctcacca
     6061 aggtcaagta cgtcaccgag ggaatgcgta agccagcctt cctctccgga gagcagaaga
     6121 aggccatcgt cgacctcctc ttcaagacca accgtaaggt caccgtcaag cagctcaagg
     6181 aggactactt caagaagatc gagtgcttcg actccgtcga gatctccgga gtcgaggacc
     6241 gtttcaacgc ctccctcgga acctaccacg acctcctcaa gatcatcaag gacaaggact
     6301 tcctcgacaa cgaggagaac gaggacatcc tcgaggacat cgtcctcacc ctcaccctct
     6361 tcgaggaccg tgagatgatc gaggtgctga agctcgaaaa tttggagctt ttacacgaga
     6421 tttttgaaga aaaagcctga aaatcgagag attttgagct gtggaattcg ttgaaattac
     6481 ataattcgcg cccgtaattc tttaaaaaaa cgctgaaatt tgcagatttt gccagaaagt
     6541 tgaagaaaaa tctacttttc cggcattctc tattattttc atggaaaatt agaaaaatcc
     6601 atgaataaac agtgaaaatt gctgatttta cctgaaattt tgaaaaaaaa aaaaatctaa
     6661 aatcaaggtt ttaagcctaa aaactgcaga tttcagccaa aattgtgaaa aaagctgatt
     6721 ttttgctatt ttgagctttt ccatggggtg gaaattgcca aaaattacgg aacattcaca
     6781 caaatttgct gaataatcca attttccagt gttttctaga gtttccgtag aaattttgaa
     6841 aaaacccagt aaattttcaa tcggaaaacc tctaaaaaca tcgtttcagg cctataaata
     6901 gtaaaaatta ccgattttat tcgaaaattt aaggaaaagt cgtgaaaaac gagattttaa
     6961 gcctcaaaac tgcagattct atctaaaatt aaaaaaaaaa aacataaata tccgaatttt
     7021 tggaggaaaa gcctgaaaat cgatatttta agcctcaaaa ctgaagattt cagccaaaaa
     7081 aaaaaacaga tttttcgcta ttttatgtgc caaaaaacca cattccgagc ttttccagag
     7141 tggaaattgc caaaaattac agaattttcc cagaaattag caaaaaaaaa acccattttt
     7201 caggcgtttt caacgatttt catagcaaat ttgaaaaatc catgaatttt ttaattatag
     7261 aaaaccctct gaaaattgca gttttaagct attccagcat tcaaatagta tggaaattgc
     7321 taaaaattga agaattttcc caaaaatttg atgaaaactt atttttctgg cgttttcgat
     7381 gattttcatg gaatttcgag aaaattaatg aattttcaat taaaaaacgt ctgcaaaatc
     7441 acatttttta aagctttttc agcaacaaaa tagtatggaa attgcttttt aaaaaagcgg
     7501 aatttttgga aaaaaggtta aattttcagt cagtttttgc actttttgac tgaaaattca
     7561 ctgaaattag ggaaaaattc cggaaaaatg attttttcga attgtagggc aaattttgca
     7621 atttcaagca atttccataa tattttaatg ctggaaaagc tcaaatttac gttttaatgg
     7681 gattttagtg aaaaattcat ggattttccg aaaaatttgc aggaaaatca tagtaaagac
     7741 ctcaaaaaat caataaaaaa tttaaaaaat tggacaatca catttctgcg taaaatctgc
     7801 actttttcag taaaaaatac cgattttccg tgaaaattct cgatttttaa tcgaaaatct
     7861 cgaaattttt acaaatttca gcaccaaaat cgttgaaaat tctgaaaatt tgaatttttt
     7921 ttccttgttt tttgagtaaa atttgcactt tttcagtaaa gaataccgat tttccgtgaa
     7981 aattctcggt ttttaatcga aaatctcgaa atttttacaa atttcagctc cgaaatagct
     8041 gaaaattgga caaaaatttc aatttttgcc ctgttttttg agtaaaatct gcacttttta
     8101 gttgaaaaaa aacacggatt ttgtaggaaa atttgggaaa aatctccaaa ttttcacaat
     8161 tttccagtaa aaacagcaat ttttaaaacg gtttttaatc aaaaatcttt agaattttcc
     8221 aaatttcagc accaaaaaag ctaaaaatcg ctgaaatttt cgacaaattc caaatttcca
     8281 ggagcgtctc aagacctacg cccacctctt cgacgacaag gtcatgaagc agctcaagcg
     8341 tcgtcgttac accggatggg gacgtctctc ccgtaagctc atcaacggaa tccgtgacaa
     8401 gcagtccgga aagaccatcc tcgacttcct caagtccgac ggattcgcca accgtaacgt
     8461 aagtttaaac atgattttac taactaacta atctgattta aattttcagt tcatgcagct
     8521 catccacgac gactccctca ccttcaagga ggacatccag aaggcccagg tctccggaca
     8581 gggagactcc ctccacgagc acatcgccaa cctcgccgga tccccagcca tcaagaaggg
     8641 aatcctccag accgtcaagg tcgtcgacga gctcgtcaag gtcatgggac gtcacaagcc
     8701 agagaacatc gtcatcgaga tggcccgtga gaaccagacc acccagaagg gacagaagaa
     8761 ctcccgtgag cgtatgaagc gtatcgagga gggaatcaag gagctcggat cccagatcct
     8821 caaggagcac ccagtcgaga acacccagct ccagaacgag aagctctacc tctactacct
     8881 ccagaacgga cgtgacatgt acgtcgacca ggagctcgac atcaaccgtc tctccgacta
     8941 cgacgtcgac cacatcgtcc cacagtcctt cctcaaggac gactccatcg acaacaaggt
     9001 cctcacccgt tccgacaaga accgtggaaa gtccgacaac gtcccatccg aggaggtcgt
     9061 caagaagatg aagaactact ggcgtcagct cctcaacgcc aagctcatca cccagcgtaa
     9121 gttcgacaac ctcaccaagg ccgagcgtgg aggactctcc gagctcgaca aggccggatt
     9181 catcaagcgt cagctcgtcg agacccgtca gatcaccaag cacgtcgccc agatcctcga
     9241 ctcccgtatg aacaccaagt acgacgagaa cgacaagctc atccgtggtt ggtttttccg
     9301 agagaaaaac gctgaaaaat gccgaaaatt ttgaatttgc aggcttttta ggtgcaaaag
     9361 tacggaaaaa tcggaaaaaa atcaggaaaa catggggaaa atccaatttt ccagaaaaaa
     9421 tgtttgaaaa aattgaaaac ttttgttttt ttagtaataa agttttaaaa aatggcaaaa
     9481 atagctgaaa acagcgaaaa aaaaaaatgt ttttttcagg aaaaaatttg caaaaaaatg
     9541 aagagcaaca ataattccct tttttttctg tgtttttttc ggaaaaattg agatcgaaaa
     9601 cgaaaaacag cgaaaattcc caattttccc agtttttaaa acattttttt gtaaaaattt
     9661 ggaaaaaacg agttttttca gaaaaaaatg tgaaaaaaaa ggtaaattac caatttatgc
     9721 ttttttttcg atcgagtaaa aaatcgattt ttccaagatt tttcgcgaaa aattcaaaaa
     9781 aaaagaacga aaattctcga ttttctactt tttttcgaaa aaaaaaggaa attatagatt
     9841 tttccaagat ttttcgcgaa aaaattcgaa aaaaatgttt ttttcgaaaa tttcagattt
     9901 tctaattttt tcagaagaat tcgaaaaaaa aaatcaaaaa attcgcaatt tttagatttt
     9961 tttaaaatct caattttcta taattatttt cctaaaaaaa tgtaattaaa aaaccgctaa
    10021 aaacggtaaa ttccaaattt ttgcattttt tcaaaaaaaa tttgaaaaaa aaaatcagaa
    10081 aattctcaaa tctattccct ttttttaaaa tttttcccaa accaaaaaaa aaaaacggta
    10141 aattttcaaa tttagcattt ttccaaaatt aaacataaaa aaaaacgcaa atttccaaat
    10201 ttcgtttttt tttcaaaaaa gttgaaaaat ccagaaaata ccctcttgtc catgaaaaaa
    10261 tacgaaaatt ttcaataaaa aatcaatttc ttcttaaaaa attaattctc gatttttcca
    10321 tgaaaaaaaa tcaaatatta attaaatttg aaaaaaaaaa gaatttatct aatagtttcg
    10381 ttttttttca gaggaaaaat ttgaaaaaaa aaacagtaaa ttctctttct ttttgaaaaa
    10441 aaaaattatc gattcttcca agttttttcc cgaaaaaatc gacaaaaaaa cgagaatttc
    10501 cgattttcta tttttttttt caaaaaaatg tgaaaaaaaa aattaaaaaa ttccaatttt
    10561 gcattttttt ttcaaaaaaa atttaaaaac accgtataat tcccgatttt tccatgaaaa
    10621 aatacgaaaa attccaataa aaatcaacta ttttaaaaat ttgccgtttt ttcagaggtc
    10681 aaggtcatca ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccagttctac
    10741 aaggtccgtg agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc
    10801 ggaaccgccc tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac
    10861 aaggtctacg acgtccgtaa gatgatcgcc aagtccgagc aggagatcgg aaaggccacc
    10921 gccaagtact tcttctactc caacatcatg aacttcttca agaccgagat caccctcgcc
    10981 aacggagaga tccgtaagcg tccactcatc gagaccaacg gagagaccgg agagatcgtc
    11041 tgggacaagg gacgtgactt cgccaccgtc cgtaaggtcc tctccatgcc acaggtcaac
    11101 atcgtcaaga agaccgaggt ccagaccgga ggattctcca aggagtccat cctcccaaag
    11161 cgtaactccg acaagctcat cgcccgtaag aaggactggg acccaaagaa gtacggagga
    11221 ttcgactccc caaccgtcgc ctactccgtc ctcgtcgtcg ccaaggtcga gaagggaaag
    11281 tccaagaagc tcaagtccgt caaggagctc ctcggaatca ccatcatgga gcgttcctcc
    11341 ttcgagaaga acccaatcga cttcctcgag gccaagggat acaaggaggt caagaaggtg
    11401 cttacaattt tctggagaaa aatattttaa aaaataacga aaatggaccg aatttttgga
    11461 gaaaatttta aaaaaaacga catttttccc aaaaaattga aaaatttccg aaaaaacgtt
    11521 caaaattttc aaaaaaggag catttttcga aaaaaaaaac gataatacgg aaattttttg
    11581 attttccagt ttttttctca aagaaaatcg gaaaaaggtt ttttttcttt aaaaacgtcc
    11641 aaaatttttc gaaaaaagag aaaattttcg attttccgat ttttttcaga aaaaaattga
    11701 aaaaaaaaga aattttccga ttttctcaga gaaagacgaa aagacgatat ttttcggaaa
    11761 aaagttgaaa aatcaaaaaa aaaaccgaaa aacggccgaa attttctaaa aattggaaat
    11821 attgtcgatt tttcgaaaaa aaagagtcaa aagttttcaa aaaaatatat tttttcgatt
    11881 ttccgttttt tttttcagta aacattgaaa caaacgaaat ttttccaaaa aaatccaatt
    11941 attttcccaa atatcgaaat atttttgatt ttccgttttt ttcagaaaaa agttaaaaaa
    12001 aaaagggaaa attaagattt tcctgatttt ttttgagaaa aaatcgataa aaattgaata
    12061 ttctggaaaa aacctttaaa aaattcgaat taaatcgaaa aacgtccaaa attttctaaa
    12121 aaattgaaat tttttttgaa aaaaaacgga aaaatttaga tttcccgact tttttcaatg
    12181 aaaaatcgaa ttttcgttga cttttcatta aaaaatccaa acgcattttt ttccactaaa
    12241 atctcgaaaa agtgtcatat tgcaccatat tgtttccaca ttcatattat tttgagctaa
    12301 atcaggtgat tttctgtatt ttgatactaa tttctatttt tagaaaaaat gtgggaacaa
    12361 tatggtgcaa tatgacattt tttcgagatt ttagtggaga aaaaatgcgt ttggaaacga
    12421 attttataat aaaaagtcaa cgaaaattcg atttttcatg gaaaaatagg gaaatcgaaa
    12481 acaaatttgc ttttttttca aaaaaaaaaa cggaaattcg aaatttttcc gttctttcaa
    12541 aaaaaaaatc gatttttttg gaaatttttt ttttcaaatt tttaaaaatt tccaggacct
    12601 catcatcaag ctcccaaagt actccctctt cgagctcgag aacggacgta agcgtatgct
    12661 cgcctccgcc ggagagctcc agaagggaaa cgagctcgcc ctcccatcca agtacgtcaa
    12721 cttcctctac ctcgcctccc actacgagaa gctcaaggga tccccagagg acaacgagca
    12781 gaagcagctc ttcgtcgagc agcacaagca ctacctcgac gagatcatcg agcagatctc
    12841 cgagttctcc aagcgtgtca tcctcgccga cgccaacctc gacaaggtcc tctccgccta
    12901 caacaagcac cgtgacaagc caatccgtga gcaggccgag aacatcatcc acctcttcac
    12961 cctcaccaac ctcggagccc cagccgcctt caagtacttc gacaccacca tcgaccgtaa
    13021 gcgttacacc tccaccaagg aggtcctcga cgccaccctc atccaccagt ccatcaccgg
    13081 actctacgag acccgtatcg acctctccca gctcggagga gactctggag gatctggagg
    13141 aactggagga tcttccgccg gcgggagctc tatgagccgt agacgaaaag cgaatccgac
    13201 aaaactgagt gaaaacgcga agaagcttgc caaggaagtt gaaaattaac acccagcttt
    13261 cttgtacaaa gtgggataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
    13321 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
    13381 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
    13441 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
    13501 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
    13561 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcacaac tttattatac
    13621 atagttgata attcactggc cgtcgtttta catggcacgc ccttctatta cagggtttct
    13681 gataacaaag ggtgccggat ctgcggctta ctcaccagac ctggccccat ccgattacca
    13741 cctattcgct tcgatgggac acgcactcgc tgagcagcgc ttcgattctt acgaaagtgt
    13801 gaaaaaatgg ctcgatgaat ggttcgccgc aaaagacgat gagttctact ggcgtggaat
    13861 ccacaaattg cccgagagat gggaaaaatg tgtagctagc gacggcaaat actttgaata
    13921 aatgattttt tctttttcca caaaatttaa cgtgtttttt gatttaaaaa aaacgacatt
    13981 tcatacttgt acacctgata tctagatacc taggtgagct ctggtacccc cctggcgtta
    14041 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    14101 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
    14161 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagtg
    14221 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    14281 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    14341 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    14401 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    14461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    14521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    14581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    14641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    14701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    14761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    14821 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    14881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    14941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    15001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    15061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    15121 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    15181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    15241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    15301 gtgggtctcg cggtatcatt gcagcactgg ggtcagatgg taagccctcc cgtatcgtag
    15361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    15421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    15481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    15541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    15601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    15661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    15721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    15781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    15841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    15901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    15961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    16021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    16081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    16141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    16201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggtcttttgc tggccttttg
    16261 ctcacatgtt ctttcctgcg ttatcccc
//
