LOCUS       sequence-370789-       18199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating Pmex-5::Cas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18199)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 18199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS544
FEATURES             Location/Qualifiers
     source          1..18199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(399..417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        485..589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(590..658,659..1450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGXDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(808..827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1621..2209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2110..2129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3845..3856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5922..5942
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6446..6470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6482..6502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(16311..16335)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      16340..16663
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(16667..16687)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(16695..16712)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16695..16711)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgctgt gacgtcgtga ctggggaaaa ccctggcgtt acccaactta atcgccttgc
       61 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      121 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      181 tctgtgcggt atttcacacc gcatatggtg cactctcagt gcaatctgct ctgatgccgc
      241 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      301 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      361 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      421 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1321 tgcagcactg gggycagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2221 cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc
     2281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg
     2341 ataacaattt cacagggccc tgtcggatta tgggctcttc ttttatcctc tactttcttt
     2401 ctagaaaatg caaatgttga gtcagaaatt gctgaatgga gtacaactgc aatgggttta
     2461 gcttatgttt ctggaacaat tactggtgga attgtgattg aaaagtaagg ttatatttca
     2521 tttcaataaa tgttaccaca gtataacaaa aacaagttac attgttcaga gtatgtctga
     2581 atgtattatt attattgtat ttgtgcatca gtactgtagt tgtactgaag aagggtttaa
     2641 aataatctca acgtttttaa gacgagttag tgagaatacg gtcgcagtta ggatgtttgg
     2701 acatagtttt attagataat cgtgaactaa ctcactataa aaacccgcta actcattgaa
     2761 taagatagtt ttaaacgttc aaaatttgaa gacaaaattg tatgaataag tttacagagt
     2821 tggtcgacgc aagcttctcc ttctcttcac atttctcaat aatttagctc taattgcatt
     2881 cgtattcttc gcaaaaatac gtattctgat tgacccgatg aaatacggat gcctagttgc
     2941 attgatcatc tacggttata cttatgggtg aggtttttca aatatatttt gatttaatcg
     3001 aataatgaaa tttcagaact ggagttggtc caatttcgtg gtttatatcc tctgaactgg
     3061 tacctcaaaa acatcgtagt attgctcaat ctgtggcata ttcaatgaat actgtaatgg
     3121 tggttatcag tacatttaca gtattacctc tatattccgt tattggtagt tatgctttcc
     3181 ttattctata ttcaattcct tcatttataa gtatgcttat attatttcgc tacttaccgg
     3241 aaaccaaagg acgagagatt catgatattg tgaatgaact taaaaaaaaa tgaaaacctt
     3301 tgaaaacgtt catacggccg aaattttact tgatcgagcc aattgaactt ttgttaagac
     3361 ttacagaaaa gtgagagatt ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga
     3421 aaaagaagaa aaatacaccc gaagtatcat ataaaaatta aaaagattgt ttgacctggc
     3481 ggaacttttt tgcaaaaact ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc
     3541 aaatgaatat caacaagaga ttcttgaaga cgacgagcca cttgctcaga gcattgctta
     3601 tctcgaatga gacccttgtt aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt
     3661 cctcctgatt ccatgatggt agcaaactca cttcgtcttt gagccaattt atccaagtcc
     3721 ttgtaaaaag tattcgaaaa ttgttaacgg ataaaatgtt tattataata tcaaaaacaa
     3781 tttgtcagtt gaccactttt tgatatattt gacagaaacc gggatgaatt ggctcaaagt
     3841 tagggcgccc ttctattaca gggtttctga taacaaaccg gttattaact cccaacaagg
     3901 gatgatttca attcatcatg ctcaaattga cccaaattaa gttacatgac aaattcatcg
     3961 cccttttcaa ctcttcttgg ctcatcatct gttattctgt tcctattctg tctgcaccct
     4021 ataccccttt gcatactctc tcgtcattcc tttcttggat agtgtcttct tctccccagc
     4081 tctgctactt ctatgacttg cgcccgctgc ttttccgctc gttcctctcc tctctgacgt
     4141 cttcttctgc ttcttttctc cttccttttt cccttctcaa tcttcctctt tttcccattt
     4201 ctgtcaatca ttcgaagaag aagaagaaga agaccctcat tcatttcatt tttttttctg
     4261 tcggtgtgtg ctgctcggtt aagagtgagc tctctattcc acgtcttctt ctttttcttc
     4321 ttcgattcga atcaatcact ccaccaaaac gcattcgttt ttgggattca ccccgcggtt
     4381 cgcaataggt ttctttttca aattattagc gttataaata gaaaaatggt ggagtttcaa
     4441 taaaaatgat aatttacaaa gtgtattttg attacatgta ctcaaaaagg ttgaaatttt
     4501 tcataccagt tttccggaaa tccatctgat atcattatcg tatttttctt tttaaaaatg
     4561 ttttcaaaaa aaaacaaaat atagctggtt atttggcacc ctctaattac cattttcctg
     4621 tcacaccaca ctcttttcct tcttccactt ctttccgttt tcagccgctt ccaaccaaac
     4681 cgatatgaaa gccgagcaac aacgatcgat tccacccggc tcggcgacct tcccgtcgca
     4741 ggtgagactc agaaaactag agaaaccgct caactaactc ttgatatccg atttcattct
     4801 tttctctttt ctttttgttg aacttttcca tatttccaga tgccacggcc accaccaagc
     4861 accgaacaag gaatcacaac ggaatcggag cttgcgaaga aagctcaaat cactccgaac
     4921 gacgttttag cacttccggg aatcactcaa ggtatcttgc tccctttgga tttcagttta
     4981 aaacataaat ttaggattct tatgctcccc atctgcgaac atctataaca tcgagttcac
     5041 caagttccaa atccgtgatc tggacactga gcaagtgctt ttcgagatcg ccaaaccgga
     5101 gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc gttatagatt
     5161 tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca aagtaggaga
     5221 catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc gccttctgaa
     5281 gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact gtgaacatat
     5341 ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa aagtacaatt
     5401 caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg agactcgttc
     5461 tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg actactcata
     5521 tgatgcataa atatttaata caaaaatgtt ctggataatt attctgtcga atagaaaaaa
     5581 aactccaaat gtgattaaat tccaataatt cctgtctagt ttgttccttc cccttcccct
     5641 tctcatgttc aatgcattcc taagcttttc agttcccccc ttgtttctat attttttcgc
     5701 tgtcctgtca cactcgctaa aaacactaat cacacggaaa tctgttttca ataaaaactc
     5761 caactttaac tcattttcaa tttcaactga aagatttttt cattagagaa tgtctagaac
     5821 taggcccggg ctacgtaata cgactcactt aaggccttga ctagagggta ccagagctca
     5881 cctaggcagg aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt
     5941 tgggttttca caacggcaaa atatcagttt ttaaaaaatt aaaccataaa acaaataata
     6001 taacccaatt tttacatcaa accacaagaa aaaaatacat ttgggcccac ggataaagaa
     6061 attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa ttcatttggg tcttaccgcg
     6121 tataccgtac tccgtttgtt tgatcatttt tgtcagcgct ggcggttgtt ttttcatttc
     6181 atttctgctt caaagacgtt ttctcgaata atttttcgtt tattctcttt tttaaaatta
     6241 atttctagcc gtaaatgtta taaattcacc catttaacgc aaatttcatg gtaatctcat
     6301 ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc aaaaattccc cgactttccc
     6361 caaaatcctg ctcgattttc cgttttctca ttgtattctc tcttaattaa ttttatcgat
     6421 aatcaattga atgtttcaga cagagacaag tttgtacaaa aaagcaggct cgatgactgc
     6481 tccaaagaag aagcgtaagg taccggtaga aaaaatggac aagaagtact ccatcggact
     6541 cgacatcgga accaactccg tcggatgggc cgtcatcacc gacgagtaca aggtcccatc
     6601 caagaagttc aaggtcctcg gaaacaccga ccgtcactcc atcaagaaga acctcatcgg
     6661 agccctcctc ttcgactccg gagagaccgc cgaggccacc cgtctcaagc gtaccgcccg
     6721 tcgtcgttac acccgtcgta agaaccgtat ctgctacctc caggagatct tctccaacga
     6781 gatggccaag gtcgacgact ccttcttcca ccgtctcgag gagtccttcc tcgtcgagga
     6841 ggacaagaag cacgagcgtc acccaatctt cggaaacatc gtcgacgagg tcgcctacca
     6901 cgagaagtac ccaaccatct accacctccg taagaagctc gtcgactcca ccgacaaggc
     6961 cgacctccgt ctcatctacc tcgccctcgc ccacatgatc aagttccgtg gacacttcct
     7021 catcgaggga gacctcaacc cagacaactc cgacgtcgac aagctcttca tccagctcgt
     7081 ccagacctac aaccagctct tcgaggagaa cccaatcaac gcctccggag tcgacgccaa
     7141 ggccatcctc tccgcccgtc tctccaagtc ccgtcgtctc gagaacctca tcgcccagct
     7201 cccaggagag aagaagaacg gactcttcgg aaacctcatc gccctctccc tcggactcac
     7261 cccaaacttc aagtccaact tcgacctcgc cgaggacgcc aagctccagc tctccaagga
     7321 cacctacgac gacgacctcg acaacctcct cgcccagatc ggagaccagt acgccgacct
     7381 cttcctcgcc gccaagaacc tctccgacgc catcctcctc tccgacatcc tccgtgtaag
     7441 gagttgaacg gctgaaaaat cgatattttg agcgaaaaaa gccggaaaaa tggattttat
     7501 cggataaaat ttgatttttt gagctgagaa atgcctttat agacgaattt ccttgtcaaa
     7561 ttgttaaaaa ctgcaaaatt tgccaaaaaa aaagcatttt ttatattttt ttttacaaaa
     7621 aattccacaa aaatggcaaa atttgactaa aaatgccaat tttgtcgttt tctccgtgcc
     7681 acagcggccg aaaatcgatt tttaagcgat tttcgggtga aaaagtgtaa aaaccgactg
     7741 aaaatccagc tgaaaacgac aaaaacggta agttttagct aattttcacc tttttttcga
     7801 tattttttta ctaaaaaaaa accgaaaaat tgagtttttt tcaaatttct gcaaaaattc
     7861 tgcaatttta gcaatttcca tactattttg acgccgaaaa agcttaaaaa tatgattttt
     7921 agaggttttt aatggaaaat tcatggattt ttcaaatttt tccacgaaaa tcattgaaaa
     7981 atttgaaaaa attgatttcc tattcaaagt tctagctaaa aactgcaatt ttagcaattt
     8041 ccatactatt ttagtactga aaaagcctaa aaatgtgatt ttcagaggat ttcaatggaa
     8101 aattcatgga tttttccgat ttttttcgat gattttcact aaaaaattgg aaaaattcat
     8161 caatttccta ttgaaaacct ctaaaaatta tatttttttt gcttttccga cgtcaaaata
     8221 gtatggaaaa tgctaaaatt gcagaatttt tccagaaatt tgaagaaaaa actcaatttt
     8281 ttccgttttt tcaatgattt tcatgaaaaa attggaaaat tttgtaaatt ttccattgaa
     8341 aacctctgaa aatttggaaa ttttcgaatt taaatgttta aaaaaaattg aatattttgc
     8401 gagtttttga attttttttg agaatttttg aatttttttc acccaaaatt ttttcaattt
     8461 ttcaggtcaa caccgagatc accaaggccc cactctccgc ctccatgatc aagcgttacg
     8521 acgagcacca ccaggacctc accctcctca aggccctcgt ccgtcagcag ctcccagaga
     8581 agtacaagga gatcttcttc gaccagtcca agaacggata cgccggatac atcgacggag
     8641 gagcctccca ggaggagttc tacaagttca tcaagccaat cctcgagaag atggacggaa
     8701 ccgaggagct cctcgtcaag ctcaaccgtg aggacctcct ccgtaagcag cgtaccttcg
     8761 acaacggatc catcccacac cagatccacc tcggagagct ccacgccatc ctccgtcgtc
     8821 aggaggactt ctacccattc ctcaaggaca accgtgagaa gatcgagaag atcctcacct
     8881 tccgtatccc atactacgtc ggaccactcg cccgtggaaa ctcccgtttc gcctggatga
     8941 cccgtaagtc cgaggagacc atcaccccat ggaacttcga ggaggtcgtc gacaagggag
     9001 cctccgccca gtccttcatc gagcgtatga ccaacttcga caagaacctc ccaaacgaga
     9061 aggtcctccc aaagcactcc ctcctctacg agtacttcac cgtctacaac gagctcacca
     9121 aggtcaagta cgtcaccgag ggaatgcgta agccagcctt cctctccgga gagcagaaga
     9181 aggccatcgt cgacctcctc ttcaagacca accgtaaggt caccgtcaag cagctcaagg
     9241 aggactactt caagaagatc gagtgcttcg actccgtcga gatctccgga gtcgaggacc
     9301 gtttcaacgc ctccctcgga acctaccacg acctcctcaa gatcatcaag gacaaggact
     9361 tcctcgacaa cgaggagaac gaggacatcc tcgaggacat cgtcctcacc ctcaccctct
     9421 tcgaggaccg tgagatgatc gaggtgctga agctcgaaaa tttggagctt ttacacgaga
     9481 tttttgaaga aaaagcctga aaatcgagag attttgagct gtggaattcg ttgaaattac
     9541 ataattcgcg cccgtaattc tttaaaaaaa cgctgaaatt tgcagatttt gccagaaagt
     9601 tgaagaaaaa tctacttttc cggcattctc tattattttc atggaaaatt agaaaaatcc
     9661 atgaataaac agtgaaaatt gctgatttta cctgaaattt tgaaaaaaaa aaaaatctaa
     9721 aatcaaggtt ttaagcctaa aaactgcaga tttcagccaa aattgtgaaa aaagctgatt
     9781 ttttgctatt ttgagctttt ccatggggtg gaaattgcca aaaattacgg aacattcaca
     9841 caaatttgct gaataatcca attttccagt gttttctaga gtttccgtag aaattttgaa
     9901 aaaacccagt aaattttcaa tcggaaaacc tctaaaaaca tcgtttcagg cctataaata
     9961 gtaaaaatta ccgattttat tcgaaaattt aaggaaaagt cgtgaaaaac gagattttaa
    10021 gcctcaaaac tgcagattct atctaaaatt aaaaaaaaaa aacataaata tccgaatttt
    10081 tggaggaaaa gcctgaaaat cgatatttta agcctcaaaa ctgaagattt cagccaaaaa
    10141 aaaaaacaga tttttcgcta ttttatgtgc caaaaaacca cattccgagc ttttccagag
    10201 tggaaattgc caaaaattac agaattttcc cagaaattag caaaaaaaaa acccattttt
    10261 caggcgtttt caacgatttt catagcaaat ttgaaaaatc catgaatttt ttaattatag
    10321 aaaaccctct gaaaattgca gttttaagct attccagcat tcaaatagta tggaaattgc
    10381 taaaaattga agaattttcc caaaaatttg atgaaaactt atttttctgg cgttttcgat
    10441 gattttcatg gaatttcgag aaaattaatg aattttcaat taaaaaacgt ctgcaaaatc
    10501 acatttttta aagctttttc agcaacaaaa tagtatggaa attgcttttt aaaaaagcgg
    10561 aatttttgga aaaaaggtta aattttcagt cagtttttgc actttttgac tgaaaattca
    10621 ctgaaattag ggaaaaattc cggaaaaatg attttttcga attgtagggc aaattttgca
    10681 atttcaagca atttccataa tattttaatg ctggaaaagc tcaaatttac gttttaatgg
    10741 gattttagtg aaaaattcat ggattttccg aaaaatttgc aggaaaatca tagtaaagac
    10801 ctcaaaaaat caataaaaaa tttaaaaaat tggacaatca catttctgcg taaaatctgc
    10861 actttttcag taaaaaatac cgattttccg tgaaaattct cgatttttaa tcgaaaatct
    10921 cgaaattttt acaaatttca gcaccaaaat cgttgaaaat tctgaaaatt tgaatttttt
    10981 ttccttgttt tttgagtaaa atttgcactt tttcagtaaa gaataccgat tttccgtgaa
    11041 aattctcggt ttttaatcga aaatctcgaa atttttacaa atttcagctc cgaaatagct
    11101 gaaaattgga caaaaatttc aatttttgcc ctgttttttg agtaaaatct gcacttttta
    11161 gttgaaaaaa aacacggatt ttgtaggaaa atttgggaaa aatctccaaa ttttcacaat
    11221 tttccagtaa aaacagcaat ttttaaaacg gtttttaatc aaaaatcttt agaattttcc
    11281 aaatttcagc accaaaaaag ctaaaaatcg ctgaaatttt cgacaaattc caaatttcca
    11341 ggagcgtctc aagacctacg cccacctctt cgacgacaag gtcatgaagc agctcaagcg
    11401 tcgtcgttac accggatggg gacgtctctc ccgtaagctc atcaacggaa tccgtgacaa
    11461 gcagtccgga aagaccatcc tcgacttcct caagtccgac ggattcgcca accgtaacgt
    11521 aagtttaaac atgattttac taactaacta atctgattta aattttcagt tcatgcagct
    11581 catccacgac gactccctca ccttcaagga ggacatccag aaggcccagg tctccggaca
    11641 gggagactcc ctccacgagc acatcgccaa cctcgccgga tccccagcca tcaagaaggg
    11701 aatcctccag accgtcaagg tcgtcgacga gctcgtcaag gtcatgggac gtcacaagcc
    11761 agagaacatc gtcatcgaga tggcccgtga gaaccagacc acccagaagg gacagaagaa
    11821 ctcccgtgag cgtatgaagc gtatcgagga gggaatcaag gagctcggat cccagatcct
    11881 caaggagcac ccagtcgaga acacccagct ccagaacgag aagctctacc tctactacct
    11941 ccagaacgga cgtgacatgt acgtcgacca ggagctcgac atcaaccgtc tctccgacta
    12001 cgacgtcgac cacatcgtcc cacagtcctt cctcaaggac gactccatcg acaacaaggt
    12061 cctcacccgt tccgacaaga accgtggaaa gtccgacaac gtcccatccg aggaggtcgt
    12121 caagaagatg aagaactact ggcgtcagct cctcaacgcc aagctcatca cccagcgtaa
    12181 gttcgacaac ctcaccaagg ccgagcgtgg aggactctcc gagctcgaca aggccggatt
    12241 catcaagcgt cagctcgtcg agacccgtca gatcaccaag cacgtcgccc agatcctcga
    12301 ctcccgtatg aacaccaagt acgacgagaa cgacaagctc atccgtggtt ggtttttccg
    12361 agagaaaaac gctgaaaaat gccgaaaatt ttgaatttgc aggcttttta ggtgcaaaag
    12421 tacggaaaaa tcggaaaaaa atcaggaaaa catggggaaa atccaatttt ccagaaaaaa
    12481 tgtttgaaaa aattgaaaac ttttgttttt ttagtaataa agttttaaaa aatggcaaaa
    12541 atagctgaaa acagcgaaaa aaaaaaatgt ttttttcagg aaaaaatttg caaaaaaatg
    12601 aagagcaaca ataattccct tttttttctg tgtttttttc ggaaaaattg agatcgaaaa
    12661 cgaaaaacag cgaaaattcc caattttccc agtttttaaa acattttttt gtaaaaattt
    12721 ggaaaaaacg agttttttca gaaaaaaatg tgaaaaaaaa ggtaaattac caatttatgc
    12781 ttttttttcg atcgagtaaa aaatcgattt ttccaagatt tttcgcgaaa aattcaaaaa
    12841 aaaagaacga aaattctcga ttttctactt tttttcgaaa aaaaaaggaa attatagatt
    12901 tttccaagat ttttcgcgaa aaaattcgaa aaaaatgttt ttttcgaaaa tttcagattt
    12961 tctaattttt tcagaagaat tcgaaaaaaa aaatcaaaaa attcgcaatt tttagatttt
    13021 tttaaaatct caattttcta taattatttt cctaaaaaaa tgtaattaaa aaaccgctaa
    13081 aaacggtaaa ttccaaattt ttgcattttt tcaaaaaaaa tttgaaaaaa aaaatcagaa
    13141 aattctcaaa tctattccct ttttttaaaa tttttcccaa accaaaaaaa aaaaacggta
    13201 aattttcaaa tttagcattt ttccaaaatt aaacataaaa aaaaacgcaa atttccaaat
    13261 ttcgtttttt tttcaaaaaa gttgaaaaat ccagaaaata ccctcttgtc catgaaaaaa
    13321 tacgaaaatt ttcaataaaa aatcaatttc ttcttaaaaa attaattctc gatttttcca
    13381 tgaaaaaaaa tcaaatatta attaaatttg aaaaaaaaaa gaatttatct aatagtttcg
    13441 ttttttttca gaggaaaaat ttgaaaaaaa aaacagtaaa ttctctttct ttttgaaaaa
    13501 aaaaattatc gattcttcca agttttttcc cgaaaaaatc gacaaaaaaa cgagaatttc
    13561 cgattttcta tttttttttt caaaaaaatg tgaaaaaaaa aattaaaaaa ttccaatttt
    13621 gcattttttt ttcaaaaaaa atttaaaaac accgtataat tcccgatttt tccatgaaaa
    13681 aatacgaaaa attccaataa aaatcaacta ttttaaaaat ttgccgtttt ttcagaggtc
    13741 aaggtcatca ccctcaagtc caagctcgtc tccgacttcc gtaaggactt ccagttctac
    13801 aaggtccgtg agatcaacaa ctaccaccac gcccacgacg cctacctcaa cgccgtcgtc
    13861 ggaaccgccc tcatcaagaa gtacccaaag ctcgagtccg agttcgtcta cggagactac
    13921 aaggtctacg acgtccgtaa gatgatcgcc aagtccgagc aggagatcgg aaaggccacc
    13981 gccaagtact tcttctactc caacatcatg aacttcttca agaccgagat caccctcgcc
    14041 aacggagaga tccgtaagcg tccactcatc gagaccaacg gagagaccgg agagatcgtc
    14101 tgggacaagg gacgtgactt cgccaccgtc cgtaaggtcc tctccatgcc acaggtcaac
    14161 atcgtcaaga agaccgaggt ccagaccgga ggattctcca aggagtccat cctcccaaag
    14221 cgtaactccg acaagctcat cgcccgtaag aaggactggg acccaaagaa gtacggagga
    14281 ttcgactccc caaccgtcgc ctactccgtc ctcgtcgtcg ccaaggtcga gaagggaaag
    14341 tccaagaagc tcaagtccgt caaggagctc ctcggaatca ccatcatgga gcgttcctcc
    14401 ttcgagaaga acccaatcga cttcctcgag gccaagggat acaaggaggt caagaaggtg
    14461 cttacaattt tctggagaaa aatattttaa aaaataacga aaatggaccg aatttttgga
    14521 gaaaatttta aaaaaaacga catttttccc aaaaaattga aaaatttccg aaaaaacgtt
    14581 caaaattttc aaaaaaggag catttttcga aaaaaaaaac gataatacgg aaattttttg
    14641 attttccagt ttttttctca aagaaaatcg gaaaaaggtt ttttttcttt aaaaacgtcc
    14701 aaaatttttc gaaaaaagag aaaattttcg attttccgat ttttttcaga aaaaaattga
    14761 aaaaaaaaga aattttccga ttttctcaga gaaagacgaa aagacgatat ttttcggaaa
    14821 aaagttgaaa aatcaaaaaa aaaaccgaaa aacggccgaa attttctaaa aattggaaat
    14881 attgtcgatt tttcgaaaaa aaagagtcaa aagttttcaa aaaaatatat tttttcgatt
    14941 ttccgttttt tttttcagta aacattgaaa caaacgaaat ttttccaaaa aaatccaatt
    15001 attttcccaa atatcgaaat atttttgatt ttccgttttt ttcagaaaaa agttaaaaaa
    15061 aaaagggaaa attaagattt tcctgatttt ttttgagaaa aaatcgataa aaattgaata
    15121 ttctggaaaa aacctttaaa aaattcgaat taaatcgaaa aacgtccaaa attttctaaa
    15181 aaattgaaat tttttttgaa aaaaaacgga aaaatttaga tttcccgact tttttcaatg
    15241 aaaaatcgaa ttttcgttga cttttcatta aaaaatccaa acgcattttt ttccactaaa
    15301 atctcgaaaa agtgtcatat tgcaccatat tgtttccaca ttcatattat tttgagctaa
    15361 atcaggtgat tttctgtatt ttgatactaa tttctatttt tagaaaaaat gtgggaacaa
    15421 tatggtgcaa tatgacattt tttcgagatt ttagtggaga aaaaatgcgt ttggaaacga
    15481 attttataat aaaaagtcaa cgaaaattcg atttttcatg gaaaaatagg gaaatcgaaa
    15541 acaaatttgc ttttttttca aaaaaaaaaa cggaaattcg aaatttttcc gttctttcaa
    15601 aaaaaaaatc gatttttttg gaaatttttt ttttcaaatt tttaaaaatt tccaggacct
    15661 catcatcaag ctcccaaagt actccctctt cgagctcgag aacggacgta agcgtatgct
    15721 cgcctccgcc ggagagctcc agaagggaaa cgagctcgcc ctcccatcca agtacgtcaa
    15781 cttcctctac ctcgcctccc actacgagaa gctcaaggga tccccagagg acaacgagca
    15841 gaagcagctc ttcgtcgagc agcacaagca ctacctcgac gagatcatcg agcagatctc
    15901 cgagttctcc aagcgtgtca tcctcgccga cgccaacctc gacaaggtcc tctccgccta
    15961 caacaagcac cgtgacaagc caatccgtga gcaggccgag aacatcatcc acctcttcac
    16021 cctcaccaac ctcggagccc cagccgcctt caagtacttc gacaccacca tcgaccgtaa
    16081 gcgttacacc tccaccaagg aggtcctcga cgccaccctc atccaccagt ccatcaccgg
    16141 actctacgag acccgtatcg acctctccca gctcggagga gactctggag gatctggagg
    16201 aactggagga tcttccgccg gcgggagctc tatgagccgt agacgaaaag cgaatccgac
    16261 aaaactgagt gaaaacgcga agaagcttgc caaggaagtt gaaaattaac acccagcttt
    16321 cttgtacaaa gtgggataaa tgcaaaatcc tttcaagcat tcccttcttc tctatcactc
    16381 ttctttcttt ttgtcaaaaa attctctcgc taatttattt gcttttttaa tgttattatt
    16441 ttatgacttt ttatagtcac tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat
    16501 gtgattctgt ctgatgtact ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa
    16561 cccgaaaggt tgagaaaaat gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc
    16621 caacaaaaag aggaaacttt attgtgccgc caagaaaaaa gtctcacaac tttattatac
    16681 atagttgata attcactggc cgtcgtttta cactcgagac gtacggtgcg cgcgatgcat
    16741 tcgaagatct gcccactagt gagtcgtatt ataagtgcaa gtaagatcag tgtttgtttc
    16801 gatatcagtc tgtttcgtaa cggtcttctg tataactaca aaaaagaata aaaaaccgta
    16861 tcacaactga gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa aattattttg
    16921 taaaaaatcg agtttttctc caaaataacg cacgcaatca aaaacaagaa aactaacgtg
    16981 ttctcctggg cgtggaactc cttatcaaga ttaccattat tgtctgtcag aattgatatt
    17041 tcaattattt catatctaga caaacccggg tgtcttgcat ttattagtac tgtgagttta
    17101 catacaaacg aggcttactc agataacata gagtcacctc taattagcaa aaacccattt
    17161 ctaattagct atttcgacca gttctaaagt caccgtaatt agaaatggtt tttgtagctt
    17221 ttctaattag aaatgacccc gagataagtg aaatatggca ttttctgggt tttctagtaa
    17281 gaaatgatcc cgcacgtagt gattccgcgt tacaagccaa attcaaatta aaaaagtata
    17341 ttacacaatt caagttacat tattttcagg aaacaatggc gaagagcaaa gcagagattc
    17401 aaaaggagga aagagacaac agatatgaac gaagacttct tgaaaagaga aagagacatg
    17461 aggtgagtaa ccatgataat aaataatcaa gtaagggcta tagatttata aattctggag
    17521 ttaacagttt cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa aataggggaa
    17581 tagcctaaac gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt ctgcagggcc
    17641 gttatccttt caaatgcact ctatcaaaca tagatcatat ccatcatatc ttatccagaa
    17701 tttatatcat atccaagatt gagagcgttt caaggaaaat ctcaaacaac catacaattt
    17761 tgtagatcga caacaaggcc tccacttctt catcgatggc ctctgcagca cctgcaccac
    17821 caagtttgta tttttcatat taatataaag tgagccacgc atagtcgttt ttaggatttt
    17881 tgccagatcg atgtcgcgca aacagactgt ttttttttct ttgaaagccg tgcgtgacat
    17941 gggccacgga ttgtaacgcc gcgctggatg gataaaaatt tattcagttt tttagccttt
    18001 acaaaatatg gttcgactac tttctttatt tttacatttt tataggtgct cattgagctg
    18061 agatattttg ggtataatcg gaaaatcgct gattttggtc atttttctgc actaaagatt
    18121 acacaaaaag ttcagtattt agattcattg ttgaaaattg gggatgatct acagatacat
    18181 atcgccgtgt tcaaaaaca
//
