LOCUS       sequence-370791-       12685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating Pmex-5::Cas9 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12685)
  AUTHORS   Schwartz ML, Davis MW, Rich MS, Jorgensen EM
  TITLE     High-efficiency CRISPR gene editing in C. elegans using Cas9 
            integrated into the genome.
  JOURNAL   PLoS Genet. 2021 Nov 8;17(11):e1009755. doi: 
            10.1371/journal.pgen.1009755. eCollection 2021 Nov.
  PUBMED    34748534
REFERENCE   2  (bases 1 to 12685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-11-8"; volume: "17(11):e1009755. doi"; pages: "
            10.1371/journal.pgen.1009755. eCollection 2021 Nov"
COMMENT     Sequence Label: pMLS546
FEATURES             Location/Qualifiers
     source          1..12685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     339..361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(399..417)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        485..589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(590..658,659..1450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(808..827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1621..2209
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2110..2129
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3845..3856)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5922..5942
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6446..6470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6482..6502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6515..8590,8642..10669)
                     /codon_start=1
                     /product="S. pyogenes Cas9, codon optimized for C. elegans 
                     with a synthetic intron"
                     /label=Cas9
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(10797..10821)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      10826..11149
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(11153..11173)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11181..11198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11181..11197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgctgt gacgtcgtga ctggggaaaa ccctggcgtt acccaactta atcgccttgc
       61 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
      121 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
      181 tctgtgcggt atttcacacc gcatatggtg cactctcagt gcaatctgct ctgatgccgc
      241 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
      301 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
      361 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
      421 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
      781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
      841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
      901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
      961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1321 tgcagcactg gggtcagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     1501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     1561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     1801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1861 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1921 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1981 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2041 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2101 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2161 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2221 cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc
     2281 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg
     2341 ataacaattt cacagggccc tgtcggatta tgggctcttc ttttatcctc tactttcttt
     2401 ctagaaaatg caaatgttga gtcagaaatt gctgaatgga gtacaactgc aatgggttta
     2461 gcttatgttt ctggaacaat tactggtgga attgtgattg aaaagtaagg ttatatttca
     2521 tttcaataaa tgttaccaca gtataacaaa aacaagttac attgttcaga gtatgtctga
     2581 atgtattatt attattgtat ttgtgcatca gtactgtagt tgtactgaag aagggtttaa
     2641 aataatctca acgtttttaa gacgagttag tgagaatacg gtcgcagtta ggatgtttgg
     2701 acatagtttt attagataat cgtgaactaa ctcactataa aaacccgcta actcattgaa
     2761 taagatagtt ttaaacgttc aaaatttgaa gacaaaattg tatgaataag tttacagagt
     2821 tggtcgacgc aagcttctcc ttctcttcac atttctcaat aatttagctc taattgcatt
     2881 cgtattcttc gcaaaaatac gtattctgat tgacccgatg aaatacggat gcctagttgc
     2941 attgatcatc tacggttata cttatgggtg aggtttttca aatatatttt gatttaatcg
     3001 aataatgaaa tttcagaact ggagttggtc caatttcgtg gtttatatcc tctgaactgg
     3061 tacctcaaaa acatcgtagt attgctcaat ctgtggcata ttcaatgaat actgtaatgg
     3121 tggttatcag tacatttaca gtattacctc tatattccgt tattggtagt tatgctttcc
     3181 ttattctata ttcaattcct tcatttataa gtatgcttat attatttcgc tacttaccgg
     3241 aaaccaaagg acgagagatt catgatattg tgaatgaact taaaaaaaaa tgaaaacctt
     3301 tgaaaacgtt catacggccg aaattttact tgatcgagcc aattgaactt ttgttaagac
     3361 ttacagaaaa gtgagagatt ttgttacaaa aaagtgagaa aaaagcgaaa atgaacagga
     3421 aaaagaagaa aaatacaccc gaagtatcat ataaaaatta aaaagattgt ttgacctggc
     3481 ggaacttttt tgcaaaaact ctgaatatcc atggcacatg cttcgtgcaa aacaggatcc
     3541 aaatgaatat caacaagaga ttcttgaaga cgacgagcca cttgctcaga gcattgctta
     3601 tctcgaatga gacccttgtt aaaatcattt ttcaaacatt atatgacatt gtcgaaatgt
     3661 cctcctgatt ccatgatggt agcaaactca cttcgtcttt gagccaattt atccaagtcc
     3721 ttgtaaaaag tattcgaaaa ttgttaacgg ataaaatgtt tattataata tcaaaaacaa
     3781 tttgtcagtt gaccactttt tgatatattt gacagaaacc gggatgaatt ggctcaaagt
     3841 tagggcgccc ttctattaca gggtttctga taacaaaccg gttattaact cccaacaagg
     3901 gatgatttca attcatcatg ctcaaattga cccaaattaa gttacatgac aaattcatcg
     3961 cccttttcaa ctcttcttgg ctcatcatct gttattctgt tcctattctg tctgcaccct
     4021 ataccccttt gcatactctc tcgtcattcc tttcttggat agtgtcttct tctccccagc
     4081 tctgctactt ctatgacttg cgcccgctgc ttttccgctc gttcctctcc tctctgacgt
     4141 cttcttctgc ttcttttctc cttccttttt cccttctcaa tcttcctctt tttcccattt
     4201 ctgtcaatca ttcgaagaag aagaagaaga agaccctcat tcatttcatt tttttttctg
     4261 tcggtgtgtg ctgctcggtt aagagtgagc tctctattcc acgtcttctt ctttttcttc
     4321 ttcgattcga atcaatcact ccaccaaaac gcattcgttt ttgggattca ccccgcggtt
     4381 cgcaataggt ttctttttca aattattagc gttataaata gaaaaatggt ggagtttcaa
     4441 taaaaatgat aatttacaaa gtgtattttg attacatgta ctcaaaaagg ttgaaatttt
     4501 tcataccagt tttccggaaa tccatctgat atcattatcg tatttttctt tttaaaaatg
     4561 ttttcaaaaa aaaacaaaat atagctggtt atttggcacc ctctaattac cattttcctg
     4621 tcacaccaca ctcttttcct tcttccactt ctttccgttt tcagccgctt ccaaccaaac
     4681 cgatatgaaa gccgagcaac aacgatcgat tccacccggc tcggcgacct tcccgtcgca
     4741 ggtgagactc agaaaactag agaaaccgct caactaactc ttgatatccg atttcattct
     4801 tttctctttt ctttttgttg aacttttcca tatttccaga tgccacggcc accaccaagc
     4861 accgaacaag gaatcacaac ggaatcggag cttgcgaaga aagctcaaat cactccgaac
     4921 gacgttttag cacttccggg aatcactcaa ggtatcttgc tccctttgga tttcagttta
     4981 aaacataaat ttaggattct tatgctcccc atctgcgaac atctataaca tcgagttcac
     5041 caagttccaa atccgtgatc tggacactga gcaagtgctt ttcgagatcg ccaaaccgga
     5101 gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc gttatagatt
     5161 tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca aagtaggaga
     5221 catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc gccttctgaa
     5281 gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact gtgaacatat
     5341 ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa aagtacaatt
     5401 caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg agactcgttc
     5461 tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg actactcata
     5521 tgatgcataa atatttaata caaaaatgtt ctggataatt attctgtcga atagaaaaaa
     5581 aactccaaat gtgattaaat tccaataatt cctgtctagt ttgttccttc cccttcccct
     5641 tctcatgttc aatgcattcc taagcttttc agttcccccc ttgtttctat attttttcgc
     5701 tgtcctgtca cactcgctaa aaacactaat cacacggaaa tctgttttca ataaaaactc
     5761 caactttaac tcattttcaa tttcaactga aagatttttt cattagagaa tgtctagaac
     5821 taggcccggg ctacgtaata cgactcactt aaggccttga ctagagggta ccagagctca
     5881 cctaggcagg aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt
     5941 tgggttttca caacggcaaa atatcagttt ttaaaaaatt aaaccataaa acaaataata
     6001 taacccaatt tttacatcaa accacaagaa aaaaatacat ttgggcccac ggataaagaa
     6061 attaaaaaaa tacatttttt aaaggcgcac cgaattaaaa ttcatttggg tcttaccgcg
     6121 tataccgtac tccgtttgtt tgatcatttt tgtcagcgct ggcggttgtt ttttcatttc
     6181 atttctgctt caaagacgtt ttctcgaata atttttcgtt tattctcttt tttaaaatta
     6241 atttctagcc gtaaatgtta taaattcacc catttaacgc aaatttcatg gtaatctcat
     6301 ggaaaaatgc agtttctttg ttaaagaaag cttaaatagc aaaaattccc cgactttccc
     6361 caaaatcctg ctcgattttc cgttttctca ttgtattctc tcttaattaa ttttatcgat
     6421 aatcaattga atgtttcaga cagagacaag tttgtacaaa aaagcaggct cgatgactgc
     6481 tccaaagaag aagcgtaagg taccggtaga aaaaatggac aagaagtact ccatcggact
     6541 cgacatcgga accaactccg tcggatgggc cgtcatcacc gacgagtaca aggtcccatc
     6601 caagaagttc aaggtcctcg gaaacaccga ccgtcactcc atcaagaaga acctcatcgg
     6661 agccctcctc ttcgactccg gagagaccgc cgaggccacc cgtctcaagc gtaccgcccg
     6721 tcgtcgttac acccgtcgta agaaccgtat ctgctacctc caggagatct tctccaacga
     6781 gatggccaag gtcgacgact ccttcttcca ccgtctcgag gagtccttcc tcgtcgagga
     6841 ggacaagaag cacgagcgtc acccaatctt cggaaacatc gtcgacgagg tcgcctacca
     6901 cgagaagtac ccaaccatct accacctccg taagaagctc gtcgactcca ccgacaaggc
     6961 cgacctccgt ctcatctacc tcgccctcgc ccacatgatc aagttccgtg gacacttcct
     7021 catcgaggga gacctcaacc cagacaactc cgacgtcgac aagctcttca tccagctcgt
     7081 ccagacctac aaccagctct tcgaggagaa cccaatcaac gcctccggag tcgacgccaa
     7141 ggccatcctc tccgcccgtc tctccaagtc ccgtcgtctc gagaacctca tcgcccagct
     7201 cccaggagag aagaagaacg gactcttcgg aaacctcatc gccctctccc tcggactcac
     7261 cccaaacttc aagtccaact tcgacctcgc cgaggacgcc aagctccagc tctccaagga
     7321 cacctacgac gacgacctcg acaacctcct cgcccagatc ggagaccagt acgccgacct
     7381 cttcctcgcc gccaagaacc tctccgacgc catcctcctc tccgacatcc tccgtgtcaa
     7441 caccgagatc accaaggccc cactctccgc ctccatgatc aagcgttacg acgagcacca
     7501 ccaggacctc accctcctca aggccctcgt ccgtcagcag ctcccagaga agtacaagga
     7561 gatcttcttc gaccagtcca agaacggata cgccggatac atcgacggag gagcctccca
     7621 ggaggagttc tacaagttca tcaagccaat cctcgagaag atggacggaa ccgaggagct
     7681 cctcgtcaag ctcaaccgtg aggacctcct ccgtaagcag cgtaccttcg acaacggatc
     7741 catcccacac cagatccacc tcggagagct ccacgccatc ctccgtcgtc aggaggactt
     7801 ctacccattc ctcaaggaca accgtgagaa gatcgagaag atcctcacct tccgtatccc
     7861 atactacgtc ggaccactcg cccgtggaaa ctcccgtttc gcctggatga cccgtaagtc
     7921 cgaggagacc atcaccccat ggaacttcga ggaggtcgtc gacaagggag cctccgccca
     7981 gtccttcatc gagcgtatga ccaacttcga caagaacctc ccaaacgaga aggtcctccc
     8041 aaagcactcc ctcctctacg agtacttcac cgtctacaac gagctcacca aggtcaagta
     8101 cgtcaccgag ggaatgcgta agccagcctt cctctccgga gagcagaaga aggccatcgt
     8161 cgacctcctc ttcaagacca accgtaaggt caccgtcaag cagctcaagg aggactactt
     8221 caagaagatc gagtgcttcg actccgtcga gatctccgga gtcgaggacc gtttcaacgc
     8281 ctccctcgga acctaccacg acctcctcaa gatcatcaag gacaaggact tcctcgacaa
     8341 cgaggagaac gaggacatcc tcgaggacat cgtcctcacc ctcaccctct tcgaggaccg
     8401 tgagatgatc gaggagcgtc tcaagaccta cgcccacctc ttcgacgaca aggtcatgaa
     8461 gcagctcaag cgtcgtcgtt acaccggatg gggacgtctc tcccgtaagc tcatcaacgg
     8521 aatccgtgac aagcagtccg gaaagaccat cctcgacttc ctcaagtccg acggattcgc
     8581 caaccgtaac gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca
     8641 gttcatgcag ctcatccacg acgactccct caccttcaag gaggacatcc agaaggccca
     8701 ggtctccgga cagggagact ccctccacga gcacatcgcc aacctcgccg gatccccagc
     8761 catcaagaag ggaatcctcc agaccgtcaa ggtcgtcgac gagctcgtca aggtcatggg
     8821 acgtcacaag ccagagaaca tcgtcatcga gatggcccgt gagaaccaga ccacccagaa
     8881 gggacagaag aactcccgtg agcgtatgaa gcgtatcgag gagggaatca aggagctcgg
     8941 atcccagatc ctcaaggagc acccagtcga gaacacccag ctccagaacg agaagctcta
     9001 cctctactac ctccagaacg gacgtgacat gtacgtcgac caggagctcg acatcaaccg
     9061 tctctccgac tacgacgtcg accacatcgt cccacagtcc ttcctcaagg acgactccat
     9121 cgacaacaag gtcctcaccc gttccgacaa gaaccgtgga aagtccgaca acgtcccatc
     9181 cgaggaggtc gtcaagaaga tgaagaacta ctggcgtcag ctcctcaacg ccaagctcat
     9241 cacccagcgt aagttcgaca acctcaccaa ggccgagcgt ggaggactct ccgagctcga
     9301 caaggccgga ttcatcaagc gtcagctcgt cgagacccgt cagatcacca agcacgtcgc
     9361 ccagatcctc gactcccgta tgaacaccaa gtacgacgag aacgacaagc tcatccgtga
     9421 ggtcaaggtc atcaccctca agtccaagct cgtctccgac ttccgtaagg acttccagtt
     9481 ctacaaggtc cgtgagatca acaactacca ccacgcccac gacgcctacc tcaacgccgt
     9541 cgtcggaacc gccctcatca agaagtaccc aaagctcgag tccgagttcg tctacggaga
     9601 ctacaaggtc tacgacgtcc gtaagatgat cgccaagtcc gagcaggaga tcggaaaggc
     9661 caccgccaag tacttcttct actccaacat catgaacttc ttcaagaccg agatcaccct
     9721 cgccaacgga gagatccgta agcgtccact catcgagacc aacggagaga ccggagagat
     9781 cgtctgggac aagggacgtg acttcgccac cgtccgtaag gtcctctcca tgccacaggt
     9841 caacatcgtc aagaagaccg aggtccagac cggaggattc tccaaggagt ccatcctccc
     9901 aaagcgtaac tccgacaagc tcatcgcccg taagaaggac tgggacccaa agaagtacgg
     9961 aggattcgac tccccaaccg tcgcctactc cgtcctcgtc gtcgccaagg tcgagaaggg
    10021 aaagtccaag aagctcaagt ccgtcaagga gctcctcgga atcaccatca tggagcgttc
    10081 ctccttcgag aagaacccaa tcgacttcct cgaggccaag ggatacaagg aggtcaagaa
    10141 ggacctcatc atcaagctcc caaagtactc cctcttcgag ctcgagaacg gacgtaagcg
    10201 tatgctcgcc tccgccggag agctccagaa gggaaacgag ctcgccctcc catccaagta
    10261 cgtcaacttc ctctacctcg cctcccacta cgagaagctc aagggatccc cagaggacaa
    10321 cgagcagaag cagctcttcg tcgagcagca caagcactac ctcgacgaga tcatcgagca
    10381 gatctccgag ttctccaagc gtgtcatcct cgccgacgcc aacctcgaca aggtcctctc
    10441 cgcctacaac aagcaccgtg acaagccaat ccgtgagcag gccgagaaca tcatccacct
    10501 cttcaccctc accaacctcg gagccccagc cgccttcaag tacttcgaca ccaccatcga
    10561 ccgtaagcgt tacacctcca ccaaggaggt cctcgacgcc accctcatcc accagtccat
    10621 caccggactc tacgagaccc gtatcgacct ctcccagctc ggaggagact ctggaggatc
    10681 tggaggaact ggaggatctt ccgccggcgg gagctctatg agccgtagac gaaaagcgaa
    10741 tccgacaaaa ctgagtgaaa acgcgaagaa gcttgccaag gaagttgaaa attaacaccc
    10801 agctttcttg tacaaagtgg gataaatgca aaatcctttc aagcattccc ttcttctcta
    10861 tcactcttct ttctttttgt caaaaaattc tctcgctaat ttatttgctt ttttaatgtt
    10921 attattttat gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat
    10981 caaaatgtga ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc
    11041 tttaaacccg aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga
    11101 gtgacccaac aaaaagagga aactttattg tgccgccaag aaaaaagtct cacaacttta
    11161 ttatacatag ttgataattc actggccgtc gttttacact cgagacgtac ggtgcgcgcg
    11221 atgcattcga agatctgccc actagtgagt cgtattataa gtgcaagtaa gatcagtgtt
    11281 tgtttcgata tcagtctgtt tcgtaacggt cttctgtata actacaaaaa agaataaaaa
    11341 accgtatcac aactgagaaa aaagacaaaa cttattgaac agtgttcctt tcaaaaaatt
    11401 attttgtaaa aaatcgagtt tttctccaaa ataacgcacg caatcaaaaa caagaaaact
    11461 aacgtgttct cctgggcgtg gaactcctta tcaagattac cattattgtc tgtcagaatt
    11521 gatatttcaa ttatttcata tctagacaaa cccgggtgtc ttgcatttat tagtactgtg
    11581 agtttacata caaacgaggc ttactcagat aacatagagt cacctctaat tagcaaaaac
    11641 ccatttctaa ttagctattt cgaccagttc taaagtcacc gtaattagaa atggtttttg
    11701 tagcttttct aattagaaat gaccccgaga taagtgaaat atggcatttt ctgggttttc
    11761 tagtaagaaa tgatcccgca cgtagtgatt ccgcgttaca agccaaattc aaattaaaaa
    11821 agtatattac acaattcaag ttacattatt ttcaggaaac aatggcgaag agcaaagcag
    11881 agattcaaaa ggaggaaaga gacaacagat atgaacgaag acttcttgaa aagagaaaga
    11941 gacatgaggt gagtaaccat gataataaat aatcaagtaa gggctataga tttataaatt
    12001 ctggagttaa cagtttcgaa aaaaagtagt tttcatatgt gcgaaggaat atcaaaaata
    12061 ggggaatagc ctaaacgagt gagaaaatta aatgtttgtt attttttcaa aaatgtctgc
    12121 agggccgtta tcctttcaaa tgcactctat caaacataga tcatatccat catatcttat
    12181 ccagaattta tatcatatcc aagattgaga gcgtttcaag gaaaatctca aacaaccata
    12241 caattttgta gatcgacaac aaggcctcca cttcttcatc gatggcctct gcagcacctg
    12301 caccaccaag tttgtatttt tcatattaat ataaagtgag ccacgcatag tcgtttttag
    12361 gatttttgcc agatcgatgt cgcgcaaaca gactgttttt ttttctttga aagccgtgcg
    12421 tgacatgggc cacggattgt aacgccgcgc tggatggata aaaatttatt cagtttttta
    12481 gcctttacaa aatatggttc gactactttc tttattttta catttttata ggtgctcatt
    12541 gagctgagat attttgggta taatcggaaa atcgctgatt ttggtcattt ttctgcacta
    12601 aagattacac aaaaagttca gtatttagat tcattgttga aaattgggga tgatctacag
    12661 atacatatcg ccgtgttcaa aaaca
//
