LOCUS       sequence-370802-       10704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The CRISPR/Cas9-and-gRNA-expressing plasmid with a 
            phleomycin-resistent selectable marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Hayat IF, Plan M, Ebert BE, Dumsday G, Vickers CE, Peng B
  TITLE     Auxin-mediated induction of GAL promoters by conditional degradation
            of Mig1p improves sesquiterpene production in Saccharomyces 
            cerevisiae with engineered acetyl-CoA synthesis.
  JOURNAL   Microb Biotechnol. 2021 Nov;14(6):2627-2642. doi: 
            10.1111/1751-7915.13880. Epub 2021 Sep 9.
  PUBMED    34499421
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1751-7915.13880"; journalName: "Microb Biotechnol"; date: 
            "2021-11"; volume: "14"; issue: "6"; pages: "2627-2642"
COMMENT     Sequence Label: pJCble
FEATURES             Location/Qualifiers
     source          1..10704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..670
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     540..563
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      699..708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             705..2423
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             4818..4838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4866..5053
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4921..4945)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(5171..5190)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(5272..5540)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(5571..5591)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5571..5589)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5610..5626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5610..5626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5623..5645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5634..5650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5658..5664,5665..5682,5683..5688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5703..5724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5841..5858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6012..6600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6092..6111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6771..7562,7563..7631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7394..7413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7632..7736)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7763..9105
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8726..8773)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9146..9164
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9202..9224)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9324..9343
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    9541..9574
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        9629..9972
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      10365..10562
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    10625..10658
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt agtgattttc
       61 ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt acatgcccaa
      121 aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga acagtttatt
      181 cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag aaaaaaaaag
      241 aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt ccattctctt
      301 agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag
      361 tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc atgtatctat
      421 ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa agctgaaaaa
      481 aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt atataaagac
      541 ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat tctactttta
      601 tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata aacacacata
      661 aacaaacaaa agatctgata tggatcgaat tagatctcgc caccatggac aagaagtatt
      721 ctatcggact ggacatcggg actaatagcg tcgggtgggc cgtcatcact gacgagtaca
      781 aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc atcaagaaaa
      841 atctgatcgg agctctcctc tttgattcag gggagaccgc tgaagcaacc cgcctcaagc
      901 ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt caagagatat
      961 tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa gaatcattcc
     1021 tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc gtcgacgagg
     1081 tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg gtggactcaa
     1141 ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatt aagttcagag
     1201 gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat aaactgttca
     1261 tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat gcaagcggag
     1321 tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgcagactt gagaatctta
     1381 tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt gctctttcac
     1441 ttgggctgac tcccaatttc aagtctaatt tcgacctggc agaggatgcc aagctgcaac
     1501 tgtccaagga cacctatgat gacgatctcg acaacctcct ggcccagatc ggtgaccaat
     1561 acgccgacct tttccttgct gctaagaatc tttctgacgc catcctgctg tctgacattc
     1621 tccgcgtgaa cactgaaatc accaaggccc ctctttcagc ttcaatgatt aagcggtatg
     1681 atgagcacca ccaggacctg accctgctta aggcactcgt ccggcagcag cttccggaga
     1741 agtacaagga aatcttcttt gaccagtcaa agaatggata cgccggctac atcgacggag
     1801 gtgcctccca agaggaattt tataagttta tcaaacctat ccttgagaag atggacggca
     1861 ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag cgcactttcg
     1921 acaatgggag cattccccac cagatccatc ttggggagct tcacgccatc cttcggcgcc
     1981 aagaggactt ctaccccttt cttaaggaca acagggagaa gattgagaaa attctcactt
     2041 tccgcatccc ctactacgtg ggacccctcg ccagaggaaa tagccggttt gcttggatga
     2101 ccagaaagtc agaagaaact atcactccct ggaacttcga agaggtggtg gacaagggag
     2161 ccagcgctca gtcattcatc gaacggatga ctaacttcga taagaacctc cccaatgaga
     2221 aggtcctgcc gaaacattcc ctgctctacg agtactttac cgtgtacaac gagctgacca
     2281 aggtgaaata tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc gaacaaaaga
     2341 aggcaattgt ggaccttctg ttcaagacca atagaaaggt gaccgtgaag cagctgaagg
     2401 aggactattt caagaaaatt gaatgcttcg actctgtgga gattagcggg gtcgaagatc
     2461 ggttcaacgc aagcctgggt acctaccatg atctgcttaa gatcatcaag gacaaggatt
     2521 ttctggacaa tgaggagaaa gaggacatcc ttgaggacat tgtcctgact ctcactctgt
     2581 tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta cgcccatctg ttcgacgata
     2641 aagtgatgaa gcaacttaaa cggagaagat ataccggatg gggacgcctt agccgcaaac
     2701 tcatcaacgg aatccgggac aaacagagcg gaaagaccat tcttgatttc cttaagagcg
     2761 acggattcgc taatcgcaac ttcatgcaac ttatccatga tgattccctg acctttaagg
     2821 aggacatcca gaaggcccaa gtgtctggac aaggtgactc actgcacgag catatcgcaa
     2881 atctggctgg ttcacccgct attaagaagg gtattctcca gaccgtgaaa gtcgtggacg
     2941 agctggtcaa ggtgatgggt cgccataaac cagagaacat tgtcatcgag atggccaggg
     3001 aaaaccagac tacccagaag ggacagaaga acagcaggga gcggatgaaa agaattgagg
     3061 aagggattaa ggagctcggg tcacagatcc ttaaagagca cccggtggaa aacacccagc
     3121 ttcagaatga gaagctctat ctgtactacc ttcaaaatgg acgcgatatg tatgtggacc
     3181 aagagcttga tatcaacagg ctctcagact acgacgtgga ccacatcgtc cctcagagct
     3241 tcctcaaaga cgactcaatt gacaataagg tgctgactcg ctcagacaag aaccggggaa
     3301 agtcagataa cgtgccctca gaggaagtcg tgaaaaagat gaagaactat tggcgccagc
     3361 ttctgaacgc aaagctaatc actcagcgga agttcgacaa tctcactaag gctgagaggg
     3421 gcggactgag cgaactggac aaagcaggat tcattaaacg gcaacttgtg gagactcggc
     3481 agattactaa acatgtagcc caaatccttg actcacgcat gaataccaag tacgacgaaa
     3541 acgacaaact tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg gtcagcgatt
     3601 tcagaaagga ctttcaattc tacaaagtgc gggagatcaa taactatcat catgctcatg
     3661 acgcatatct gaatgccgtg gtgggaaccg ccctaatcaa gaagtaccca aagctggaaa
     3721 gcgagttcgt gtacggagac tacaaggtct acgacgtgcg caagatgatt gccaaatctg
     3781 agcaggagat cggaaaggcc accgcaaagt acttcttcta cagcaacatc atgaatttct
     3841 tcaagaccga aatcaccctt gcaaacggtg agatccggaa gaggccgctc atcgagacta
     3901 atggggagac tggcgaaatc gtgtgggaca agggcagaga tttcgctacc gtgcgcaaag
     3961 tgctttctat gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc ggaggctttt
     4021 ctaaggaatc aatcctcccc aagcgcaact ccgacaagct cattgcaagg aagaaggatt
     4081 gggaccctaa gaagtacggc ggattcgatt caccaactgt ggcttattct gtcctggtcg
     4141 tggctaaggt ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa ctgctgggta
     4201 tcaccattat ggagcgcagc tccttcgaga agaacccaat tgactttctc gaagccaaag
     4261 gttacaagga agtcaagaag gaccttatca tcaagctccc aaagtatagc ctgttcgaac
     4321 tggagaatgg gcggaagcgg atgctcgcct ccgctggcga acttcagaag ggtaatgagc
     4381 tggctctccc ctccaagtac gtgaatttcc tctaccttgc aagccattac gagaagctga
     4441 aggggagccc cgaggacaac gagcaaaagc aactgtttgt ggagcagcat aagcattatc
     4501 tggacgagat cattgagcag atttccgagt tttctaaacg cgtcattctc gctgatgcca
     4561 acctcgataa agtccttagc gcatacaata agcacagaga caaaccaatt cgggagcagg
     4621 ctgagaatat catccacctg ttcaccctca ccaatcttgg tgcccctgcc gcattcaagt
     4681 acttcgacac caccatcgac cggaaacgct atacctccac caaagaagtg ctggacgcca
     4741 ccctcatcca ccagagcatc accggacttt acgaaactcg gattgacctc tcacagctcg
     4801 gaggggatga gggagctccc aagaaaaagc gcaaggtagg tagttccgga tcctagactc
     4861 tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata aaaaaaataa
     4921 gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta ttcttgagta
     4981 actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct cttattgacc
     5041 acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc tctagagcgg
     5101 ccgtggtatc gtttagattg gcaattacag tgtcttagct cacatgctta taactaatta
     5161 catgactcga agacataaaa aacaaaaaaa gcaccaccga ctcggtgcca ctttttcaag
     5221 ttgataacgg actagcctta ttttaacttg ctatttaaat gcatcgattc tgatcattta
     5281 tctttcactg cggagaagtt tcgaacgccg aaacatgcgc accaactttc acttctacag
     5341 cgtttgacca aaatcttttg aacagaacat tgtagggtgt gaaaaaatgc gcacctttac
     5401 cgctagccca agagggcact acaaaatcta gagttgtact tcaaacgtac atgtaatcac
     5461 cttgtatata ctcgaaagaa aacatcaagt ttctgtataa atatgagtga aagcataatc
     5521 atacattatc ttttcaaaga ttgtccgcgg tggagctcca gcttttgttc cctttagtga
     5581 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5701 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5761 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5821 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5881 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5941 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6001 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6061 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7741 tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg
     7801 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     7861 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     7921 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     7981 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     8041 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     8101 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     8161 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     8221 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     8281 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     8341 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     8401 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     8461 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     8521 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     8581 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     8641 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
     8701 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     8761 gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc
     8821 gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat
     8881 acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt
     8941 tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta
     9001 tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg
     9061 attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat
     9121 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     9181 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     9241 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     9301 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac
     9361 caccttttca attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat
     9421 ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga
     9481 ttggtatata tacgcatatg cagctgaagc ttcgtacgct gcaggtcgac aacccttaat
     9541 ataacttcgt ataatgtatg ctatacgaag ttattaggtc tagagatctg tttagcttgc
     9601 ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag
     9661 tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca
     9721 tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa
     9781 ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa
     9841 ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt
     9901 cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga
     9961 acataaacaa ccatgggtat gaccgaccaa gcgacgccca acctgccatc acgagatttc
    10021 gatcccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    10081 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc cgggctcgat
    10141 cccctcgcga gttggttcag ctgctgcctg aggctggacg acctcgcgga gttctaccgg
    10201 cagtgcaaat ccgtcggcat ccaggaaacc agcagcggct atccgcgcat ccatgccccc
    10261 gaactgcagg agtggggagg cacgatggcc gctttggtcg acccggacgg gacgctcctg
    10321 cgcctgatac agaacgaatt gcttgcaggc atctcatgat cagtactgac aataaaaaga
    10381 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
    10441 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
    10501 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
    10561 tgctgtcgat tcgatactaa cgccgccatc cagtgtcgaa aacgagctct cgagaaccct
    10621 taatataact tcgtataatg tatgctatac gaagttatta ggtgatatca gatccactag
    10681 tggcctatac tagtcagttc gagt
//
