LOCUS       sequence-370806-       11098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin 
            B-resistent selectable marker .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11098)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJCHph
FEATURES             Location/Qualifiers
     source          1..11098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        14..680
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     550..573
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     regulatory      709..718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             715..2433
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             4828..4848
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      4876..5063
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4931..4955)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      complement(5181..5200)
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        complement(5282..5550)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     primer_bind     complement(5581..5601)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5581..5599)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5620..5636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5620..5636)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5633..5655)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5644..5660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5668..5674,5675..5692,5693..5698))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5713..5734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5851..5868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6102..6121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6781..7572,7573..7641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7404..7423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7642..7746)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7773..9115
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8736..8783)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9156..9174
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9212..9234)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9334..9353
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             9841..10869
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             9841..10869
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      10875..11072
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
       61 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
      121 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
      181 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
      241 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
      301 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
      361 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
      421 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
      481 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
      541 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
      601 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
      661 aacacacata aacaaacaaa agatctgata tggatcgaat tagatctcgc caccatggac
      721 aagaagtatt ctatcggact ggacatcggg actaatagcg tcgggtgggc cgtcatcact
      781 gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc
      841 atcaagaaaa atctgatcgg agctctcctc tttgattcag gggagaccgc tgaagcaacc
      901 cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt
      961 caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa
     1021 gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc
     1081 gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg
     1141 gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatt
     1201 aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat
     1261 aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat
     1321 gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgcagactt
     1381 gagaatctta tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt
     1441 gctctttcac ttgggctgac tcccaatttc aagtctaatt tcgacctggc agaggatgcc
     1501 aagctgcaac tgtccaagga cacctatgat gacgatctcg acaacctcct ggcccagatc
     1561 ggtgaccaat acgccgacct tttccttgct gctaagaatc tttctgacgc catcctgctg
     1621 tctgacattc tccgcgtgaa cactgaaatc accaaggccc ctctttcagc ttcaatgatt
     1681 aagcggtatg atgagcacca ccaggacctg accctgctta aggcactcgt ccggcagcag
     1741 cttccggaga agtacaagga aatcttcttt gaccagtcaa agaatggata cgccggctac
     1801 atcgacggag gtgcctccca agaggaattt tataagttta tcaaacctat ccttgagaag
     1861 atggacggca ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag
     1921 cgcactttcg acaatgggag cattccccac cagatccatc ttggggagct tcacgccatc
     1981 cttcggcgcc aagaggactt ctaccccttt cttaaggaca acagggagaa gattgagaaa
     2041 attctcactt tccgcatccc ctactacgtg ggacccctcg ccagaggaaa tagccggttt
     2101 gcttggatga ccagaaagtc agaagaaact atcactccct ggaacttcga agaggtggtg
     2161 gacaagggag ccagcgctca gtcattcatc gaacggatga ctaacttcga taagaacctc
     2221 cccaatgaga aggtcctgcc gaaacattcc ctgctctacg agtactttac cgtgtacaac
     2281 gagctgacca aggtgaaata tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc
     2341 gaacaaaaga aggcaattgt ggaccttctg ttcaagacca atagaaaggt gaccgtgaag
     2401 cagctgaagg aggactattt caagaaaatt gaatgcttcg actctgtgga gattagcggg
     2461 gtcgaagatc ggttcaacgc aagcctgggt acctaccatg atctgcttaa gatcatcaag
     2521 gacaaggatt ttctggacaa tgaggagaaa gaggacatcc ttgaggacat tgtcctgact
     2581 ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta cgcccatctg
     2641 ttcgacgata aagtgatgaa gcaacttaaa cggagaagat ataccggatg gggacgcctt
     2701 agccgcaaac tcatcaacgg aatccgggac aaacagagcg gaaagaccat tcttgatttc
     2761 cttaagagcg acggattcgc taatcgcaac ttcatgcaac ttatccatga tgattccctg
     2821 acctttaagg aggacatcca gaaggcccaa gtgtctggac aaggtgactc actgcacgag
     2881 catatcgcaa atctggctgg ttcacccgct attaagaagg gtattctcca gaccgtgaaa
     2941 gtcgtggacg agctggtcaa ggtgatgggt cgccataaac cagagaacat tgtcatcgag
     3001 atggccaggg aaaaccagac tacccagaag ggacagaaga acagcaggga gcggatgaaa
     3061 agaattgagg aagggattaa ggagctcggg tcacagatcc ttaaagagca cccggtggaa
     3121 aacacccagc ttcagaatga gaagctctat ctgtactacc ttcaaaatgg acgcgatatg
     3181 tatgtggacc aagagcttga tatcaacagg ctctcagact acgacgtgga ccacatcgtc
     3241 cctcagagct tcctcaaaga cgactcaatt gacaataagg tgctgactcg ctcagacaag
     3301 aaccggggaa agtcagataa cgtgccctca gaggaagtcg tgaaaaagat gaagaactat
     3361 tggcgccagc ttctgaacgc aaagctaatc actcagcgga agttcgacaa tctcactaag
     3421 gctgagaggg gcggactgag cgaactggac aaagcaggat tcattaaacg gcaacttgtg
     3481 gagactcggc agattactaa acatgtagcc caaatccttg actcacgcat gaataccaag
     3541 tacgacgaaa acgacaaact tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg
     3601 gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc gggagatcaa taactatcat
     3661 catgctcatg acgcatatct gaatgccgtg gtgggaaccg ccctaatcaa gaagtaccca
     3721 aagctggaaa gcgagttcgt gtacggagac tacaaggtct acgacgtgcg caagatgatt
     3781 gccaaatctg agcaggagat cggaaaggcc accgcaaagt acttcttcta cagcaacatc
     3841 atgaatttct tcaagaccga aatcaccctt gcaaacggtg agatccggaa gaggccgctc
     3901 atcgagacta atggggagac tggcgaaatc gtgtgggaca agggcagaga tttcgctacc
     3961 gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc
     4021 ggaggctttt ctaaggaatc aatcctcccc aagcgcaact ccgacaagct cattgcaagg
     4081 aagaaggatt gggaccctaa gaagtacggc ggattcgatt caccaactgt ggcttattct
     4141 gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa
     4201 ctgctgggta tcaccattat ggagcgcagc tccttcgaga agaacccaat tgactttctc
     4261 gaagccaaag gttacaagga agtcaagaag gaccttatca tcaagctccc aaagtatagc
     4321 ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct ccgctggcga acttcagaag
     4381 ggtaatgagc tggctctccc ctccaagtac gtgaatttcc tctaccttgc aagccattac
     4441 gagaagctga aggggagccc cgaggacaac gagcaaaagc aactgtttgt ggagcagcat
     4501 aagcattatc tggacgagat cattgagcag atttccgagt tttctaaacg cgtcattctc
     4561 gctgatgcca acctcgataa agtccttagc gcatacaata agcacagaga caaaccaatt
     4621 cgggagcagg ctgagaatat catccacctg ttcaccctca ccaatcttgg tgcccctgcc
     4681 gcattcaagt acttcgacac caccatcgac cggaaacgct atacctccac caaagaagtg
     4741 ctggacgcca ccctcatcca ccagagcatc accggacttt acgaaactcg gattgacctc
     4801 tcacagctcg gaggggatga gggagctccc aagaaaaagc gcaaggtagg tagttccgga
     4861 tcctagactc tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata
     4921 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
     4981 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
     5041 cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc
     5101 tctagagcgg ccgtggtatc gtttagattg gcaattacag tgtcttagct cacatgctta
     5161 taactaatta catgactcga agacataaaa aacaaaaaaa gcaccaccga ctcggtgcca
     5221 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttaaat gcatcgattc
     5281 tgatcattta tctttcactg cggagaagtt tcgaacgccg aaacatgcgc accaactttc
     5341 acttctacag cgtttgacca aaatcttttg aacagaacat tgtagggtgt gaaaaaatgc
     5401 gcacctttac cgctagccca agagggcact acaaaatcta gagttgtact tcaaacgtac
     5461 atgtaatcac cttgtatata ctcgaaagaa aacatcaagt ttctgtataa atatgagtga
     5521 aagcataatc atacattatc ttttcaaaga ttgtccgcgg tggagctcca gcttttgttc
     5581 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5641 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5701 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5761 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5821 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5881 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     5941 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6181 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6421 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7081 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7381 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7741 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
     7801 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     7861 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
     7921 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     7981 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
     8041 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
     8101 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
     8161 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
     8221 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
     8281 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
     8341 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
     8401 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
     8461 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
     8521 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
     8581 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
     8641 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
     8701 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagagaat
     8761 aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa
     8821 cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt
     8881 atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg cgtacttata
     8941 tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt atcccattcc
     9001 atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata tgctgccact
     9061 cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt ggatcatact
     9121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     9181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     9241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     9301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     9361 tgcaccatac caccttttca attcatcatt ttttttttat tctttttttt gatttcggtt
     9421 tccttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca
     9481 cagacttaga ttggtatata tacgcatatg gaataccctc cttgacagtc ttgacgtgcg
     9541 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
     9601 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
     9661 gctgcggacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
     9721 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
     9781 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc
     9841 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     9901 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     9961 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    10021 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    10081 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
    10141 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
    10201 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
    10261 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
    10321 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
    10381 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
    10441 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
    10501 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
    10561 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
    10621 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
    10681 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
    10741 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
    10801 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
    10861 aaggaataat cagtactgac aataaaaaga ttcttgtttt caagaacttg tcatttgtat
    10921 agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt tatatttttt
    10981 ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta atatcatgcg
    11041 tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa cgactagt
//
