LOCUS       sequence-370811-       11794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocking out ALD6; expressing acetylating aldehyde dehydrogenase 
            (Lactobacillus reuteri pduP and L. reuteri EutE) and an 
            NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11794)
  AUTHORS   Hayat IF, Plan M, Ebert BE, Dumsday G, Vickers CE, Peng B
  TITLE     Auxin-mediated induction of GAL promoters by conditional degradation
            of Mig1p improves sesquiterpene production in Saccharomyces 
            cerevisiae with engineered acetyl-CoA synthesis.
  JOURNAL   Microb Biotechnol. 2021 Nov;14(6):2627-2642. doi: 
            10.1111/1751-7915.13880. Epub 2021 Sep 9.
  PUBMED    34499421
REFERENCE   2  (bases 1 to 11794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1751-7915.13880"; journalName: "Microb Biotechnol"; date: 
            "2021-11"; volume: "14"; issue: "6"; pages: "2627-2642"
COMMENT     Sequence Label: pIALD2HMGr
FEATURES             Location/Qualifiers
     source          1..11794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3310..3898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3390..3409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4069..4860,4861..4929))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4692..4711
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4930..5034)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5061..6403
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6024..6071)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     6444..6462
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6500..6522)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6622..6641
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6662..6943
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             6944..7618
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     terminator      9971..10241
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     promoter        10348..11052
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 atgcaagatg aaggtgcatt tgttgttaac cgtgaacagg ctgacaaact cgccgatatg
       61 tgcatccaag acaacggtac tcctgatcgg aagtttgttg gaaaggatgc aacctacatc
      121 ttagatcaag ctaatattcc atatactggt cacccggtag aaattatttg cgaattgcca
      181 aaggaacacc cattggtaat gactgaaatg ttgatgccaa tcttaccagt tgtttcttgc
      241 ccaacatttg atgatgtttt gaagactgct gttgaagtag aacaaggaaa ccaccatact
      301 gcaactattc actccaacaa cttaaagcat attaataatg ctgctcaccg gatgcaatgt
      361 tcgatcttcg ttgttaacgg tccatcctat gttggaacag gtgttgctga taatggtgct
      421 cactcaggtg cttcagcatt aacaattgct acgccaactg gtgaaggaac ttgtactgca
      481 agaaccttta cgcgtcgtgt tcgtttgaat tcgccacaag gattctcagt tcgtaattgg
      541 tactaagcga tttaatctct aattattagt taaagtttta taagcatttt tatgtaacga
      601 aaaataaatt ggttcatatt attactgcac tgtcacttac catggaaaga ccagacaaga
      661 agttgccgac acgacagtct gttgaattgg cttaagtctg ggtccgcttc tttacaaatt
      721 tgaagaattt ctcttaaacg atatgtatat tcttttcgtt ggaaaagatg tcttccaaaa
      781 aaaaaaaccg atgaattagt ggaaccaagg aaaaaaaaag aggtatcctt gattaaggaa
      841 cagcggccgc ccacaaccgc accagatccg gaagacacag agaacaggag attaccagat
      901 ccagatcagc agaacaccgg aacaaaggcc ggaaggactc caagcagccg cacacctgat
      961 ttgtagccag tgagttcgca cctaactcca ataaagtgca gaataaatcc tgcattaaca
     1021 cactgagagt cgggccttgg acggccgatt atagtccttt tgtcgccata aaaagtcccc
     1081 tcgaggtcac gtgcgcgaag gaaccccccc tgagcagctt ttcgcccaga aatcaaggaa
     1141 ggggcggcac cagcaggcac cggcggccac tagtccgtgc ggcggggtct gtgacattca
     1201 ggggccagcg tgcctttagt gatatcgccg cagcgcgcaa actcggagag gaccactccg
     1261 agcagaagtt agttaagcaa ttctctacgc taaaaaaaaa agtaaagtgg attaagctcc
     1321 tactggagag ttaagtatgt gtacacttcc agatatgcac aacgtgctag gcaactattg
     1381 ttctaaggag agatgtggtg cggagtaaaa tgcggaggta gtatggggct tgcagacata
     1441 tataaagtct agtcattcgg ctgaaaataa actagaaact acttcttccc tttccatcta
     1501 catcccactc atccaacaat atcaacaata caatagtaat ataatataca cacattcgca
     1561 tctagaaaaa tgttgattaa cgatattgaa agtgctgttc gcaagatcct agcagaagaa
     1621 ttagataatg ctagctctaa cagtgcggca gttactaatg atgatggaca tcgtggtatt
     1681 tttactaatg ttgatgacgc aattgctgct gctaaagctg cccaagaagt atatcgtgac
     1741 aagccaattg cagtgcggca acaggtgatt gatgctatca aggaaggttt ccgtccttac
     1801 attgaaaaga tggcaaagga cattaaagaa gaaaccggaa tgggaacagt cgaagcaaag
     1861 attgctaagt tgcacaatgc tttgtacaac actcctggtc cagaaattct tgaaccggtt
     1921 gtcgaaaatg gtgatggcgg aatggttatg tatgaacggt tgccatacgg tgttatcggt
     1981 gctgttggtc caagtactaa cccatccgaa acagttattg ccaatgcaat tatgatgctt
     2041 gcaggtggaa atacccttta ctttggtgct caccctggtg ctaagaatat cactcgttgg
     2101 acgattgaaa agatgaatga tttcattgct gatgcaactg gtcttcacaa tttagttgta
     2161 agcattgaaa caccaacaat tgaatctgtt caacaaatga tgcaacatcc tgatattgct
     2221 atgcttgccg taaccggtgg accagctgtt gttcaccaag caatgactag cggtaagaag
     2281 gctgttggtg ctggccctgg taatcctcct gcaatggttg atgctaccgc tgatattgac
     2341 ttagctgctc ataatattat tgcttcagct tcatttgata atgacatttt atgtactgct
     2401 gaaaaggaag ttgttgccga aagtagtatt aaggatgaat taatccgcaa gatgcaagat
     2461 gaaggtgcat ttgttgttaa ccgtgaacag gctgacaaac tcgccgatat gtgcatccaa
     2521 gacaacggta ctcctgatcg gaagtttgtt ggaaaggatg caacctacat cttagatcaa
     2581 gctaatattc catatactgg tcacccggta gaaattattt gcgaattgcc aaaggaacac
     2641 ccattggtaa tgactgaaat gttgatgcca atcttaccag ttgtttcttg cccaacattt
     2701 gatgatgttt tgaagactgc tgttgaagta gaacaaggaa accaccatac tgcaactatt
     2761 cactccaaca acttaaagca tattaataat gctgctcacc ggatgcaatg ttcgatcttc
     2821 gttgttaacg gtccatccta tgttggaaca ggtgttgctg ataatggtgc tcactcaggt
     2881 gcttcagcat taacaattgc tacgccaact ggtgaaggaa cttgtactgc aagaaccttt
     2941 acgcgtcgtg ttcgtttgaa ttcgccacaa gggttctcag ttcgtaattg gtactaactg
     3001 cagtgtacca acctgcattt ctttccgtca tatacacaaa atactttcat ataaacttac
     3061 ttggtcttac gtcataaata aatatgtata catataaatt aaaaaatttg gttttatatt
     3121 tttacaaaaa gaatcgttta cttcatttct cccttttaag cgatacaatc catgaaaaaa
     3181 gagaaaaaga gagaacaggc ttgtgccttc tttaaaacat cccacacaaa atcatattga
     3241 attgaattta aattgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5041 tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca
     5101 acgcgagagc gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat
     5161 gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa
     5221 atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca
     5281 gaaatgcaac gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta
     5341 caaaaatgca tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct
     5401 cctttgtgcg ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg
     5461 ttagaagaag gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt
     5521 cccgcgttta ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc
     5581 cgattatatt ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt
     5641 gatgattctt cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact
     5701 acgtatagga aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta
     5761 ctacaatttt tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga
     5821 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
     5881 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
     5941 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
     6001 cttttggttt tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga
     6061 ataggaactt caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc
     6121 acatacagct cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac
     6181 atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta
     6241 tgtaggatga aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc
     6301 gtatgcttcc ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat
     6361 tagtctcatc cttcaatgct atcatttcct ttgatattgg atcatactaa gaaaccatta
     6421 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     6481 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     6541 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     6601 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatagat
     6661 caacgacatt actatatata taatatagga agcatttaat agaacagcat cgtaatatat
     6721 gtgtactttg cagttatgac gccagatggc agtagtggaa gatattcttt attgaaaaat
     6781 agcttgtcac cttacgtaca atcttgatcc ggagcttttc tttttttgcc gattaagaat
     6841 taattcggtc gaaaaaagaa aaggagaggg ccaagaggga gggcattggt gactattgag
     6901 cacgtgagta tacgtgatta agcacacaaa ggcagcttgg agtatgtctg ttattaattt
     6961 cacaggtagt tctggtccat tggtgaaagt ttgcggcttg cagagcacag aggccgcaga
     7021 atgtgctcta gattccgatg ctgacttgct gggtattata tgtgtgccca atagaaagag
     7081 aacaattgac ccggttattg caaggaaaat ttcaagtctt gtaaaagcat ataaaaatag
     7141 ttcaggcact ccgaaatact tggttggcgt gtttcgtaat caacctaagg aggatgtttt
     7201 ggctctggtc aatgattacg gcattgatat cgtccaactg catggagatg agtcgtggca
     7261 agaataccaa gagttcctcg gtttgccagt tattaaaaga ctcgtatttc caaaagactg
     7321 caacatacta ctcagtgcag cttcacagaa acctcattcg tttattccct tgtttgattc
     7381 agaagcaggt gggacaggtg aacttttgga ttggaactcg atttctgact gggttggaag
     7441 gcaagagagc cccgaaagct tacattttat gttagctggt ggactgacgc cagaaaatgt
     7501 tggtgatgcg cttagattaa atggcgttat tggtgttgat gtaagcggag gtgtggagac
     7561 aaatggtgta aaagactcta acaaaatagc aaatttcgtc aaaaatgcta agaaataggt
     7621 tattactgag tagtatttat ttaagtattg tttgtgcact tgccgatcta tgcggtgtga
     7681 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaag cgttaatatt
     7741 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     7801 atcggcaaaa tcccttaatt taaatgtaat aagaagtttg gtaatattca attcgaagtg
     7861 ttcagtcttt tacttctctt gttttataga agaaaaaaca tcaagaaaca tctttaacat
     7921 acacaaacac atactatcag aatacaatga ctaagctaca ctttgacact gctgaaccag
     7981 tcaagaggat ccatgtttta tttgtagagt tcaatttttc tctcttactt caaaaatgta
     8041 gtgagctgta tcgtcacact tttacggagc gctattatca taagcacgga tatttctagt
     8101 cttacttata tgttagagaa attaccaata ccccttagta ccttaaccaa aagagaaagg
     8161 acctttcaat aaggctaaaa ctcaacgctt agctaccaat ttctttgcaa tgttgaagaa
     8221 cggatcagaa actgccgagc ggacgacagc tctccgacgg gatgctctac tccgcgcgcc
     8281 attgtcttca ttagttgtgt ccctgaaaac aaacatatct cgcaccgcca tgctccgaac
     8341 aataaagagt gaaagagttg agggcaaact cccctggttt cacgtcccgt gtaaccaatg
     8401 gaacaagttc attcgcatac gatggtatcg aaattagctt tgacatgctt ctatgatggt
     8461 tctgtacaag tgggtacttt ccttccagca aagatataaa tgctaaagta aaattgatat
     8521 agttgcactg atttttcatt ccgttcactt ttttagttgc atagtttctg attttaggtt
     8581 gctttaagat aaagaaaata gcaaacaaca aatatttgat atctgtttat acattactca
     8641 taaaagataa aaataaaaat ctataaagct taaaatggtt gctgattcca gattgccaaa
     8701 tttcagagct ttgactccag ctcaaagaag agatttcttg gctgatgctt gtggtttgtc
     8761 tgatgctgaa agagctttgt tagctgctcc aggtgctttg ccattggctt tagctgatgg
     8821 tatgattgaa aatgtttttg gttcttttga attgccatta ggtgttgctg gtaactttag
     8881 agttaatggt agagatgttt tggttccaat ggctgttgaa gaaccatctg ttgttgctgc
     8941 tgcttcttat atggctaagt tggctagaga agatggtggt tttcaaactt cttctacttt
     9001 gccattgatg agagcacaag ttcaagtttt gggtgttact gatccacatg gtgctagatt
     9061 ggctgtttta caagctagag cccaaattat tgaaagagcc aactcaagag ataaagtctt
     9121 gattggttta ggtggtggtt gtaaagatat tgaagttcat gtttttccag atactccaag
     9181 aggtccaatg ttggttgttc atttgattgt tgatgttaga gatgctatgg gtgctaatac
     9241 tgttaacact atggctgaat ctgttgctcc attagttgaa aagattactg gtggttctgt
     9301 tagattgaga atcttgtcta atttggctga tttgagatta gccagagcta gagttagatt
     9361 gactccacaa actttggcta cccaagatag atcaggtgaa gaaatcattg aaggtgtttt
     9421 ggatgcttat acttttgctg ctattgatcc atatagagct gctactcata acaagggtat
     9481 tatgaatggt attgatccag ttattgttgc tactggtaat gattggagag cagttgaagc
     9541 tggtgctcat gcttatgctt ccagatcagg ttcttatact tctttgacta gatgggaaaa
     9601 agatgctggt ggtgctttgg ttggttctat tgaattacca atgccagttg gtttggttgg
     9661 tggtgctact aaaactcatc cattagctag attagctttg aaaatcatgg acttgcaatc
     9721 tgctcaacaa ttgggtgaaa ttgcagctgc tgttggtttg gctcaaaatt tgggtgcttt
     9781 gagagcttta gctactgaag gtattcaaag aggtcacatg gctttacatg ctagaaatat
     9841 tgctttagtt gctggtgcta caggtgacga agttgatgct gttgctagac aattagctgc
     9901 tgaacatgat gttagaactg atagagcttt agaagttttg gctgcattaa gagcaagagc
     9961 ttaacccggg attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc
    10021 catcctttac gctaaaataa tagtttattt tattttttga atatttttta tttatatacg
    10081 tatatataga ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc
    10141 gctcctcttt taatgccttt atgcagtttt tttttcccat tcgatatttc tatgttcggg
    10201 ttcagcgtat tttaagttta ataactcgaa aattctgcgt tcgttaaagc tttcgagaag
    10261 gatattattt cgaaataaac cgtgttgtgt aagcttgaag gatccgctct tttccgattt
    10321 ttttctaaac cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga
    10381 cttcctcttt tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc
    10441 tccctaacat gtaggtggcg gaggggagat atacaataga acagatacca gacaagacat
    10501 aatgggctaa acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac
    10561 taatactgta gccctagact tgatagccat catcatatcg aagtttcact accctttttc
    10621 catttgccat ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt
    10681 tctctctccc ccgttgttgt ctcaccatat ccgcaatgac aaaaaaatga tggaagacac
    10741 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
    10801 aggggtatct cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
    10861 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg
    10921 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
    10981 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
    11041 atacaatcaa ctatctcata tacaggtacc aaaatgcaga ttaacgatat tgaaagtgct
    11101 gtacgcaaaa ttcttgcaga agaattagat aatgctagct ctaacagtgc ggcagttact
    11161 aatgatgatg gacatcgtgg tatttttact aatgttgatg acgcaattgc tgctgctaaa
    11221 gctgcccaag aagtatatcg tgacaagcca attgcagtgc ggcaacaggt gattgatgct
    11281 atcaaggaag gtttccgtcc ttacattgaa aagatggcaa aggacattaa agaagaaacc
    11341 ggaatgggaa cagtcgaagc aaagattgct aagttgcaca atgctttgta caacactcct
    11401 ggtccagaaa ttcttgaacc ggttgtcgaa aatggtgatg gcggaatggt tatgtatgaa
    11461 cggttgccat acggtgttat cggtgctgtt ggtccaagta ctaacccatc cgaaacagtt
    11521 attgccaatg caattatgat gcttgcaggt ggaaataccc tttactttgg tgctcaccct
    11581 ggtgctaaga atatcactcg ttggacgatt gaaaagatga atgatttcat tgctgatgca
    11641 actggtcttc acaatttagt tgtaagcatt gaaacaccaa caattgaatc tgttcaacaa
    11701 atgatgcaac atcctgatat tgctatgctt gccgtaaccg gtggaccagc tgttgttcac
    11761 caagcaatga ctagcggtaa gaaggctgtt ggtg
//
