LOCUS       sequence-370814-       13413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knocking out GPP1 and expressing a phosphoketolase pathway 
            (Lactobacillus reuteri xfp and Methanosarcina barkeri pta).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Hayat IF, Plan M, Ebert BE, Dumsday G, Vickers CE, Peng B
  TITLE     Auxin-mediated induction of GAL promoters by conditional degradation
            of Mig1p improves sesquiterpene production in Saccharomyces 
            cerevisiae with engineered acetyl-CoA synthesis.
  JOURNAL   Microb Biotechnol. 2021 Nov;14(6):2627-2642. doi: 
            10.1111/1751-7915.13880. Epub 2021 Sep 9.
  PUBMED    34499421
REFERENCE   2  (bases 1 to 13413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1751-7915.13880"; journalName: "Microb Biotechnol"; date: 
            "2021-11"; volume: "14"; issue: "6"; pages: "2627-2642"
COMMENT     Sequence Label: pIPKAH
FEATURES             Location/Qualifiers
     source          1..13413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5985..6005)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5985..6003)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6024..6040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6024..6040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6037..6059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6048..6064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6072..6078,6079..6096,6097..6102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6117..6138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6255..6272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6426..7014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6506..6525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7185..7976,7977..8045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7808..7827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8046..8150)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8177..9519
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9140..9187)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9560..9578
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9616..9638)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9738..9757
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9778..10059
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             10060..10734
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10837..11292)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10974..10995)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11186..11205
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11418..11440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11432..11449
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11433..11449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11459..11478
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11459..11477
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12122..13150
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASIAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      13156..13353
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tagaaggtgg attaacaaat ataatgacaa atcgttgctt gtctgacttg attccactac
       61 agttacaaat atttgacatt gtatataagt tttgcaagtt catcaaatct atgagagcaa
      121 aattatgtca actggacccc gtactatatg agaaacacaa aagcgggatg atgaaaacac
      181 taaacgaagg ctatcgtaca aacaatggcg gtcaggaaga tgttggttac caagaagatg
      241 ccgccctgga attaattcag aagctgattg aatacattag caacgcgtcc agcatttttc
      301 ggaagtgtct cataaacttt actcaagagt taagtactga aaaattcgac ttttatgata
      361 gttcaagtgt cgacgctgcg ggtatagaaa gggttcttta ctctatagta cctcctcgct
      421 cagcatctgc ttcttcccaa agatgaacgc ggcgttatgt cactaacgac gtgcaccaac
      481 ttgcggaaag tggaatcccg ttccaaaact ggcatccact aattgataca tctacacacc
      541 gcacgccttt tttctgaagc ccacttggat ccaaaatggc tgttgattac gattccaaga
      601 agtatttgga aagtgttgat gcttactggc gtgcagctaa ctacctttca gttggtactt
      661 tgttcttaat gggtgatcca ttattacgcc aaccattaaa ggctgaagac gttaagccta
      721 agccaattgg tcactggggt actattgttc ctcaaaactt catttacgca cacttaaacc
      781 gtgtaattaa gaagtatgac cttgatatgt tctacattga aggttcaggt cacggtggtc
      841 aagttatggt taacaactca tacttggatg gttcatacac tgaaatttat cctgaataca
      901 cacaagacac taagggaatg gctaagttat tcaagcactt ctcattccca ggcggtactg
      961 catctcacgc cgcacctgaa actccaggtt caatccacga aggtggggaa cttggttact
     1021 cactttcaca cggtgtaggt gctatcttag ataaccctga tgttatcgcc gctgttgaaa
     1081 ttggtgatgg tgaagctgaa actggtccat taatggcatc atggttctca gacaagttca
     1141 tcaacccaat caaggatggt gcggtattac caatcatcca agttaacgga ttcaagattt
     1201 ctaaccctac tattctttca cggatgagtg acgaagaact tactaagtac tttgaaggta
     1261 tgggctggaa gccatacttc gtatcagctt acaaagaagc tgaccgtgat ggtgaattcg
     1321 atggttacaa gccacacatg gaagttcacg aacaaatggc taagactatg gataaggctg
     1381 ttgaagaaat caaggctatc caaaagaacg ctcgtgaaaa caacgatgat tcattaccac
     1441 aatggccatt aatcatcttc cgtgcaccta agggttggac tggtcctact aaggatcttg
     1501 atggtaaccc aatcgaaaac tcattccgtg ctcaccaaat tcctattccg gtatcacaag
     1561 atgacatgga acacaaggac atgcttatta actggatgaa gtcatacaag ccagaagaat
     1621 tgtttgacga aaacggtcac ccagttgctc ttgttgaaga gaacacacca gaaggtaacc
     1681 gtcgtatggc tatgaatcct atcactaatg gtggtatcga tcctaagcca cttgtattac
     1741 caaactaccg tgactttgct gttgacgttc aaactccagg ttctgttgta aagcaagata
     1801 tgcttgaatg gggtaagtac ctcagcaaga tggctgaatt gaacccaact aacttccgtg
     1861 gatttggtcc tgacgaatct aagtcaaacc gtctttacgc attccttgac aaccaaaagc
     1921 gtcaatggat ggaaggcatt cacgaaccaa acgatgaaaa cgttgctcca caaggtcgga
     1981 tgattgattc tcaattatca gaacaccaag ctgaaggatt ccttgaaggt tacacattaa
     2041 ctggtcgtca cggattcttc gcaacttatg aagcattcgg tcgtgttgtt gattcaatgc
     2101 ttactcaaca catgaagtgg ttacgtaagg ctaaggatct ttactggcgt cgtcaatacc
     2161 cagcattgaa ctttgttgat acttcaactg tattccaaca agatcacaac ggttacactc
     2221 accaagatcc aggtctattg actcacttat ttgaaaagga acgtccagac cttgttaagg
     2281 aatacttacc tgctgatact aactcactta tggctgtatc aaacaaggca ttccgtaacc
     2341 aagaatgtat caacctcttt gtaacttcaa aacacccacg tgcacaatgg ttctccattg
     2401 atgaagctac tgaattagtt gacaatggtc ttggctacat caactgggca tctactgacc
     2461 aaggtagcga accagatgtt gtatttgctt cagctggtac tgaacctact gaagaagctc
     2521 ttgcagctat tgacattctt catgacaact tccctgaatt gaagattcgt tacatcaaca
     2581 tcattgaaat catgaagtta atgaagcaag acaagaaccc agaaggtttg tctgacgcag
     2641 aattcaaccg ttacttcact actgacaagc cagttatctt tgcatggcat ggattccgtg
     2701 acatgatcca agcattgttc ttcgatcgtg caaaccgtaa cgttcacatt cactcatacg
     2761 aagaaaatgg tgacatcacc actccattcg acatgcgtgt attaaacgaa cttgaccggt
     2821 tccacttagc taaggatgct atccaaagcg ttcctggtta cgaacaaaag agtgctgcat
     2881 ttgttgccaa gatggacaac atgattaaca agcacaacca ctacattcgt tcagaaggta
     2941 aggacttacc agaagttact aactggactt ggaagggtct taagtaagct agcgattaat
     3001 ataattatat aaaaatatta tcttcttttc tttatatcta gtgttatgta aaataaattg
     3061 atgactacgg aaagcttttt tatattgttt ctttttcatt ctgagccact taaatttcgt
     3121 gaatgttctt ataagggacg gtagatttac aagtgataca acaaaaagca aggcgctttt
     3181 tctaataaaa agaagaaaag catttaacaa ttgaacacct ctatatcaac gaagaatatt
     3241 actttgtctc taaatccttg taaaatgtgt acgatctcta tatgggttac tcagaagtgt
     3301 accgaagact gcattgaaag tttatgtttt ttcactggag gcgtcagcgg ccgcctattt
     3361 tcgaggacct tgtcaccttg agcccaagag agccaagatt taaattttcc tatgacttga
     3421 tgcaaattcc caaagctaat aacatgcaag acacgtacgg tcaagaagac atatttgacc
     3481 tcttaacagg ttcagacgcg actgcctcat cagtaagacc cgttgaaaag aacttacctg
     3541 aaaaaaacga atatatacta gcgttgaatg ttagcgtcaa caacaagaag tttaatgacg
     3601 cggaggccaa ggcaaaaaga ttccttgatt acgtaaggga gttagaatca ttttgaataa
     3661 aaaacacgct ttttcagttc gagtttatca ttatcaatac tgccatttca aagaatacgt
     3721 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
     3781 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacatcgt
     3841 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
     3901 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcactgcag
     3961 aaaatggtta cttttttgga aaagatttct gaaagagcaa agaaattgaa caaaacaatt
     4021 gctttacctg aaactactga tattagaact ttgcaggctg ctgctaaagc attagaaaga
     4081 ggtgttgcta acattgtttt gatcggtgac gaagctaaca tcaaagagtt ggcaggagat
     4141 ttagacttgt caaaagctaa aattgttaat ccagagactt atgagaagaa agatgagtac
     4201 attcaagcat tctatgagtt gagaaaacat aagggtatta cattggaatc tgctgctgaa
     4261 atcatgaagg attacgttta ctttgctgtt atggctgcta aattgaatga agttgatggt
     4321 gtcgtttctg gtgctgtaca ctcttcctct gatactttga gaccagctgt tcagattgtt
     4381 aaaactgctc cagatgctgc tttggcttct gcttttttca ttattgctgt tccagattgt
     4441 gaatatggtt ccgaaggtac attcttgttt gctgattctg gtatggttga aatgccttct
     4501 ccagaagatg ttgcaaacat tgctatcatc tctgcaaaga cattcgaatt gttggtccag
     4561 gacgacccat acgttgctat gttgtcatac tcaactaaag gttctgctca ttccaagttg
     4621 actgaagcta ctattgctgc cactaaattg gctcaagagt tggctcctga catccctatc
     4681 gacggtgaat tgcaggttga tgctgctatt gttccaaaag ttgcagcttc taaagctcca
     4741 ggttctccag ttgcaggtaa agctaatgtc tttatctttc cagatttgaa cgctggtaac
     4801 atcgcttaca agattgctca aaggttggct aaagctgagg cttatggtcc tattactcaa
     4861 ggattagcta aaccaattaa tgatttgtca agaggttgtt ctgatgaaga cattgttggt
     4921 gctattgcta tcacttgtgt tcaagcagct gctcaggaca agtaactcga ggttaattca
     4981 aattaattga tatagttttt taatgagtat tgaatctgtt tagaaataat ggaatattat
     5041 ttttatttat ttatttatat tattggtcgg ctcttttctt ctgaaggtca atgacaaaat
     5101 gatatgaagg aaataatgat ttctaaaatt ttacaacgta agatattttt acaaaagcct
     5161 agctcatctt ttgtcatgca ctattttact cacgcttgaa attaacggcc agtccactgc
     5221 ggagtcattt caaagtcatc ctaatcgatc tatcgttttt gatagctcat tttggagttc
     5281 gcgattgtct tctgttattc acaactgttt tatctagatt ttcttttatt tttttgataa
     5341 aactactacg ctaaaaataa aataaaaatg tatgatttcc ctccatttcc gaccaattgt
     5401 ataattttat atctgcatga cttaataata taatataata cttataaaat acgaatagaa
     5461 aaatttaaac cgatgtaatg catccttttc tttgttgtct tcggatgatc tgccgtgaca
     5521 ggtggttcgc gcaaatcaaa ctggattaga gaatttaaca cagaaataaa aaaggaagat
     5581 tcaatcttcg tttttgtttt atatcttact ataaaagtgt ttttttttta gtacgacgag
     5641 aagttagagt aattataaaa ggaatgctta tttaaattta tttcttagac ttcttttcag
     5701 acttcttagc agcctcagtt tgttccttaa cgaccttctt aacaatcttt tgttcctcaa
     5761 tcaagaaagc tctgacgatt ctttccttga cacagttggc acatctggaa ccaccgtaag
     5821 ctctggaaac agtcttgtgg gtcttggaga cagtagcgta ttgtcttggt ctcaaagtgg
     5881 aaataccttg tagagcacta ccacagtcac cacactttgg tctagtagcc aacttcttaa
     5941 cgtgttgggc acgcaagata ccacctgagc tccagctttt gttcccttta gtgagggtta
     6001 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     7741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8161 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     8221 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     8281 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     8341 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     8401 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     8461 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     8521 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     8581 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     8641 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     8701 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     8761 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     8821 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
     8881 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
     8941 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
     9001 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
     9061 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
     9121 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
     9181 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
     9241 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
     9301 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
     9361 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
     9421 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
     9481 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
     9541 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     9601 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     9661 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     9721 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac
     9781 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt
     9841 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct
     9901 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat
     9961 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg
    10021 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca
    10081 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt
    10141 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca
    10201 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca
    10261 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct
    10321 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa
    10381 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac
    10441 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa
    10501 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa
    10561 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt
    10621 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat
    10681 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt
    10741 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata
    10801 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
    10861 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10921 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    10981 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    11041 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    11101 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    11161 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    11221 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    11281 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11341 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11401 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
    11461 atacgactca ctatagggcg aattgactag tcttttttcc tttttttttc ttttcttact
    11521 ctgttaatca ctttctttcc tttttcttca tattgtcgtc ttgttcattt tcgttcaatt
    11581 gataatgtat ataaatcttt cctaagtatc tcttgattgc catttttttc tttccaagtt
    11641 tccttgttat gaaacgtttc aatgttttaa aatatatcag aacaacaaaa gcaaatatac
    11701 aaaccatcgc aatgcctttg accacaaaac ctttatcttt gaaaatcaac gccgctctat
    11761 tcgatgttga cggtaccatc atcatctctc agaataccct ccttgacagt cttgacgtgc
    11821 gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat ccccatgtat
    11881 aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat tacggctcct
    11941 cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg
    12001 ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc ttctttcata
    12061 tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa cataaacaac
    12121 catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
    12181 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
    12241 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
    12301 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
    12361 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
    12421 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
    12481 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
    12541 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    12601 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccatcgcgc aggctctcga
    12661 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    12721 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    12781 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    12841 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    12901 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    12961 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    13021 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    13081 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
    13141 aaaggaataa tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta
    13201 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
    13261 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
    13321 gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgagatcta
    13381 atcctactct tgccgttgcc atccaaaatg agc
//
