LOCUS       sequence-370829-       11379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of nerolidol synthetic module at
            RPL25 locus (11 copy). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11379)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pINER2R
FEATURES             Location/Qualifiers
     source          1..11379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(826..842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(826..842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(839..861)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    850..866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(874..880,881..898,899..904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    919..940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1057..1074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1228..1816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1308..1327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1987..2778,2779..2847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2610..2629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2848..2952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3020..3038
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3076..3098)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3198..3217
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3411..3433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3425..3442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3426..3442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5488..5929
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    5488..5605
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5900..5923
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             8872..9246
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
ORIGIN
        1 cgtaatgatt tgcatgctgc gatactgccg tagcgggcct tcgtatagct cggccgagct
       61 cgtacaaaag gcaagcagtg tatcggacag agctgatata acacaatacg ctcgtagtcg
      121 atgcatgccg tggctgctct cggtcgggta taagtcttag acaatagtct tacctcgcat
      181 gtataataaa tcttttgtat ttaatctatt atatgtttct atgctttttt ttcctattgt
      241 tgtttgcttt tccttttcct tatttctttc tagcttctaa ttttctttct tttttttttt
      301 tttttcattg aaaattatat atatatatat atatcagaac aattgtccag tattgaacaa
      361 tacaggttat ttcgaacaat tgaaaaaaaa aaatcacaga aaaacatatc gagaaaaggg
      421 tcatggctcc atctggtatg tgaactgcaa tattaatagc acgagaaaat tgagaggaag
      481 atagatggga actagtagag ttgatattga tgagatacga aaaccacacg taaataaact
      541 atccgacgac aagaatagtg cattaaggac ttgattaaga tatggtgagc aacgtaatta
      601 tcgggctcaa cagtttatta gcaatcgttt tgatagaagc gttgatgctg tggaagttgt
      661 tctttttact accaacagcc attaacaaat ccattagagt gttcgttcgt ttttctgctc
      721 agattcaaga aaattattcc atcctcatta tacttttttc ttctatttcg tgctccacgt
      781 cgaggtatca aggaacatag tttaaacaag cttggcgtaa tcatggtcat agctgtttcc
      841 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      901 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      961 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     1081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     1261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1501 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1561 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1621 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1681 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1741 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1801 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1861 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1921 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1981 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2041 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2101 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     2161 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2221 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2281 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2341 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     2401 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2461 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2521 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2581 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     2641 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2701 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2761 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2821 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2881 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2941 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     3001 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     3061 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     3121 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     3181 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3241 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     3301 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3361 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3421 acgttgtaaa acgacggcca gtgaattcag tttaaacatg tactaatcag tctaacaccc
     3481 cggaggaggc ttatcattac atattcttgt ctcggcgttc tttaagtttc ttatcttgta
     3541 tgcccgatat agcaaccttg ttggtaccaa tctaacggtt tccgtacttt gcaatgaaga
     3601 gatgaggagg catgggtcac ttatttaata tgtacgggtg tttacatgga gttgctttct
     3661 ttttttgtct cagcagtcat tgtgcgccaa aaaaagagaa aaccgtgagc cgaagtccac
     3721 gctctggagt taggctctcc cattacggag agaagcattt cctcagcctg ggagccccgt
     3781 tggaacagtc aggctaaact gggccttcct acccactgct tgctgtttct cactggacgc
     3841 acaaggggat tttctttcta ccttcggctt gcctcactgc gttggggctt cccaatgcaa
     3901 cttcgttcgt atgcatacaa tcttttagat attatctttt aaaattattt taaaacaatt
     3961 ttaaatgtat ctcatatgct tttcttctgc tgttgaaaag gctaaacaaa gaagatcaat
     4021 aagataaagc ggccgcaaaa tgtctaagaa tatcgttgtc ctaccgggtg atcacgtcgg
     4081 taaagaagtt actgacgaag ctattaaggt cttgaatgcc attgctgaag tccgtccaga
     4141 aattaagttc aatttccaac atcacttgat cgggggtgct gccatcgatg ccactggcac
     4201 tcctttacca gatgaagctc tagaagcctc taagaaagcc gatgctgtct tactaggtgc
     4261 tgttggtggt ccaaaatggg gtacgggcgc agttagacca gaacaaggtc tattgaagat
     4321 cagaaaggaa ttgggtctat acgccaactt gagaccatgt aactttgctt ctgattcttt
     4381 actagatctt tctcctttga agcctgaata tgcaaagggt accgatttcg tcgtcgttag
     4441 agaattggtt ggtggtatct actttggtga aagaaaagaa gatgaaggtg acggagttgc
     4501 ttgggactct gagaaataca gtgttcctga agttcaaaga attacaagaa tggctgcttt
     4561 cttggcattg caacaaaacc caccattacc aatctggtct cttgacaagg ctaacgtgct
     4621 tgcctcttcc agattgtgga gaaagactgt tgaagaaacc atcaagactg agttcccaca
     4681 attaactgtt cagcaccaat tgatcgactc tgctgctatg attttggtta aatcaccaac
     4741 taagctaaac ggtgttgtta ttaccaacaa catgtttggt gatattatct ccgatgaagc
     4801 ctctgttatt ccaggttctt tgggtttatt accttctgca tctctagctt ccctacctga
     4861 cactaacaag gcattcggtt tgtacgaacc atgtcatggt tctgccccag atttaccagc
     4921 aaacaaggtt aacccaattg ctaccatctt atctgcagct atgatgttga agttatcctt
     4981 ggatttggtt gaagaaggta gggctcttga agaagctgtt agaaatgtct tggatgcagg
     5041 tgtcagaacc ggtgaccttg gtggttctaa ctctaccact gaggttggcg atgctatcgc
     5101 caaggctgtc aaggaaatct tggcttaaag agtcttttgt aacgaccccg tctccaccaa
     5161 cttggtatgc ttgaaatctc aaggccatta cacattcagt tatgtgaacg aaaggtcttt
     5221 atttaacgta gcataaacta aataatacag gttccggtta gcctgcgcta gcttatattg
     5281 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt
     5341 acatggcaat accaccatat acatatccat atctaatctt acttatatgt tgtggaaatg
     5401 taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc
     5461 ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg cgacagccct
     5521 ccgacggaag actctcctcc gtgcgtcctg gtcttcaccg gtcgcgttcc tgaaacgcag
     5581 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
     5641 tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatca acgaatcaaa
     5701 ttaacaacca taggataata atgcgattag ttttttagcc ttatttctgg ggtaattaat
     5761 cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaagc tgcataacca
     5821 ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa tgtcataaaa
     5881 gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga aaaaactata
     5941 gctagcaaaa tggcttcaga aaaagaaatt aggagagaga gattcttgaa cgttttccct
     6001 aaattagtag aggaattgaa cgcatcgctt ttggcttacg gtatgcctaa ggaagcatgt
     6061 gactggtatg cccactcatt gaactacaac actccaggcg gtaagttaaa tagaggtttg
     6121 tccgttgtgg acacgtatgc tattctctcc aacaagaccg ttgaacaatt ggggcaagaa
     6181 gaatacgaaa aggttgctat tctaggttgg tgcattgagt tgttgcaggc ttacttcttg
     6241 gtcgccgatg atatgatgga caagtccatt accagaagag gccaaccatg ttggtacaag
     6301 gttcctgaag ttggggaaat tgccatcaat gacgcattca tgttagaggc tgctatctac
     6361 aagcttttga aatctcactt cagaaacgaa aaatactaca tagatatcac cgaattgttc
     6421 catgaagtca ccttccaaac cgaattgggc caattgatgg acttaatcac tgcacctgaa
     6481 gacaaagtcg acttgagtaa gttctcccta aagaagcact ccttcatagt tactttcaag
     6541 actgcttact attctttcta cttgcctgtc gcattggcta tgtacgttgc cggtatcaca
     6601 gatgaaaagg atttgaaaca agccagagat gtcttgattc cattgggtga atatttccaa
     6661 attcaagatg actacttaga ctgcttcggt accccagaac agatcggtaa gatcggtaca
     6721 gatatccaag ataacaaatg ttcttgggta atcaacaagg cattagaact tgcttccgca
     6781 gaacaaagaa agactttaga cgaaaattac ggtaagaagg actcagtcgc agaagccaaa
     6841 tgcaaaaaga ttttcaatga cttgaaaatc gaccagttat accacgaata tgaagagtct
     6901 gttgccaagg atttgaaggc caagatctcc caagtcgacg agtctcgtgg cttcaaagcc
     6961 gacgtcttaa ctgcgttttt gaacaaagtt tacaagagaa gcaaataata gtgacctgca
     7021 ggaagttttg ttagaaaata aatcattttt taattgagca ttcttattcc tattttattt
     7081 aaatagtttt atgtattgtt agctacatac aacagtttaa atcaaatttt ctttttccca
     7141 agtccaaaat ggaggtttat tttgatgacc cgcatgcgat tatgttttga aagtataaga
     7201 ctacatacat gtacatatat ttaaacatgt aaacccgtcc attatattgc ttactttctt
     7261 cttttttgcc gttttgactt ggacctctgg tttgctattt ccttacaatc tttgctacaa
     7321 tgggcccaaa gctagctcta attggtttaa ttaataaatt taatattatt tttaaatttt
     7381 tctttaaata tacaataaat ctttcataac atgttaaatt catgattaag cgtaaataaa
     7441 gtgtagtggc agagtgcacg gggtttcctg tgccttacaa agtaggtacc aatttgcgta
     7501 ttgcagcgag ggttccggtt actatttata attacgtgtt agtgtactgt gattttattg
     7561 aggctataac aagaaaagga tctgtgaagg ttttgagaga atgaggcgaa aacacagggt
     7621 tattttcatc agcaacgccg ctgtttcagc ggcaccctgc ccttttcttt tcgcaagatg
     7681 agagtaaaga gttgtacatc aggtaagaat gttattattt aaattcgaag tgataaattc
     7741 ttttcatgat gaatcactcg cttatatggg gtagaatata tatatatgtg tgtgtgtgtg
     7801 tgtgtttgtg tatgtaggga tggtgcgcgt ttgttgtgtg acatttgcta ctcattcttt
     7861 tccttttcct acgactggct taacgggaat attatcaatt tgctgcattc ttatgcttcg
     7921 gtccgatgct caagcttaac ttcttcgtga ggaaggaaag tgttgtctcc tacttttttc
     7981 aaattttcga attgtattta tatttattta gtacttcttg agtttacata tccttcgtaa
     8041 aaatgcaact tttgtcgaaa aacacttcca aaaaaaaata ataatgaatt tatgaagcat
     8101 actaacgagc gagcacatcg ctgaggtatc attacttcat gagataaatt aactcgagtg
     8161 tactaatcca aggaggttta cggaccagag gaactttcca gattcagatc acagcaatat
     8221 aggactggaa aacatcaggt agccgcactc aacttgtaac tggcaactac tttgcattaa
     8281 actccaatta aatgcggtag aatcttttca gaaaaggtat tcaacgtcaa ttcggaaagc
     8341 ttccttccgg aatggcttaa gtaggttgca atttcttttt ctattagtag ctaaaaatgg
     8401 gtcacgtgat ctatattcga aaggggcggt tgcctcagga aggcaccggc ggtctttcgt
     8461 ccgtgcggag atatctgcgc cgttcagggg tccatgtgcc ttggacgata ttaaggcaga
     8521 aggcagtatc ggggcggatc actccgaacc gagattagtt aagcccttcc catctcaaga
     8581 tggggagcaa atggcattat actcctgcta gaaagttaac tgtgcacata ttcttaaatt
     8641 atacaatgtt ctggagagct attgtttaaa aaacaaacat ttcgcaggct aaaatgtgga
     8701 gataggatta gttttgtaga catatataaa caatcagtaa ttggattgaa aatttggtgt
     8761 tgtgaattgc tcttcattat gcaccttatt caattatcat caagaatagc aatagttaag
     8821 taaacacaag attaacataa taaaaaaaat aattctttca gtggatccaa aatggaacac
     8881 gttgctttcg gttctgaaga tattgaaaac actttggcta aaatggatga tggtcaatta
     8941 gacggtttag ctttcggtgc tattcaattg gacggtgatg gtaacatttt gcaatacaat
     9001 gctgctgaag gtgatattac tggtagagat ccaaaacaag ttattggtaa gaacttcttt
     9061 aaggacgttg ctccaggtac tgactctcca gagttctatg gtaagtttaa ggaaggtgtt
     9121 gcttctggta acttaaacac tatgtttgaa tggatgatcc caacttccag aggtccaact
     9181 aaggttaaag ttcacatgaa aaaggcttta tctggtgact cttactgggt tttcgttaaa
     9241 agagttggtg ctactaactt ctctttgttg aagttggctg gtgacgttga acttaaccca
     9301 ggtccagcta ccgcagcagg tcctatcgca actaacaact ccccacaaaa ctccaacgct
     9361 tacagaactc caatcgctcc ttccgtacca attactcata aatggtctat agctgaagat
     9421 ttgacatgta tttccaatcc tagtaagcac aataaccctc aaactggtta cagatcattt
     9481 tctgacgaat tatacgttaa gtacgaagaa aagttggaag atgttagaaa agcattaaga
     9541 gaagttgaag aaaacccttt ggaaggttta gttatgatag acgctttgca aagattgggt
     9601 atcgattacc atttcagagg tgaaattggt gcattcttgc aaaagcaaca aatcatatct
     9661 tcaactccag atggttaccc tgaacatggt ttgtacgaag tttcaacatt gtttagattc
     9721 ttaagacaag aaggtcacaa tgttaccgct gacgtcttta ataacttcaa ggataaggaa
     9781 ggtagattca gatcagaatt gtcaacagat attagaggtt tgatgtcctt atacgaagca
     9841 agtcaattga gaatagaagg tgaagacatc ttagatcaag ctgctgattt ctccagtcaa
     9901 ttgttaggta gatggacaaa agatcctaat catcacgaag ccagattggt ttctaacact
     9961 ttaacacatc cataccacaa gtcattggct acctttatgg gtcaaaaatt gtcctacatg
    10021 aactgcaagg gtccaaactg ggacggtgtc gataatttgc aagaattagc taagatggat
    10081 ttgactatcg tacaaagtat ccatcaaaag gaagtattcc aagtttctca atggtggaag
    10141 gatacaggtt tagccaatga attgaaattg gctagaaacc aaccattgaa gtggtatatg
    10201 tggcctatgg ccgctttaac cgatccaaga ttcagtgaag aaagagttga attaactaag
    10261 cctatttctt ttatatacat catagatgac atcttcgacg tctatggtac cattgaagaa
    10321 ttgaccttgt ttactgatgc agttaataga tgggaattgt ctgccgtcga acaattacca
    10381 gactacatga aagtatgttt caaggctttg tacgatgtta ccaacgaaat cgcatacaaa
    10441 atctataaaa agcatggtca aaaccctatt gattccttgc aaaagacttg ggctagtttg
    10501 tgcaatgcat ttttagttga agcaaagtgg ttcgcctctg gtcacttgcc aaatgcagaa
    10561 gaatacttaa agaacggtat catctcttca ggtgttcatg ttgtcttggc ccacatgttt
    10621 ttcttgttag gtgacggtat tacacaagaa tcagttgatt tggtagatga ctatccaggt
    10681 atttccacaa gtatcgcaac cattttgaga ttatctgatg acttgggttc agccaaagat
    10741 gaagaccaag atggttatga tggttcttac atcgaatgtt acatgaagga acataagggt
    10801 tccagtgtcg attcagccag agaagaagta ataagaatga tctccgaagc atggaaatgt
    10861 ttgaataagg aatgcttatc accaaaccct ttttctgaat cattcagaat aggttccttg
    10921 aatatggcta gaatgatccc tatgatgtac tcttacgatg acaaccataa cttgccaatt
    10981 ttagaagaac acatgaaggc aatgatctat aacacatctt tataatagtg accatggcgg
    11041 attgagagca aatcgttaag ttcaggtcaa gtaaaaattg atttcgaaaa ctaatttctc
    11101 ttatacaatc ctttgattgg accgtcatcc tttcgaatat aagattttgt taagaatatt
    11161 ttagacagag atctacttta tatttaatat ctagatatta cataatttcc tctctaataa
    11221 aatatcatta ataaaataaa aatgaagcga tttgattttg tgttgtcaac ttagtttgcc
    11281 gctatgcctc ttgggtaatg ctattattga atcgaagggc tttattatat taccctttag
    11341 cttattctga ggtttctgtg gcgtcccggg aatctcggt
//
