LOCUS       sequence-370835-       11715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of limonene synthetic module at 
            RPL25 locus (14 copy). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11715)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIT6EG7ml
FEATURES             Location/Qualifiers
     source          1..11715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2856..3230)
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     primer_bind     complement(6602..6619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6602..6618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6611..6633)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6827..6846)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6946..6968
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7006..7024)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7092..7196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7197..7265,7266..8057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7415..7434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8228..8816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8717..8736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8970..8987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9104..9125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9140..9145,9146..9163,9164..9170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9178..9194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9183..9205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9202..9218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9202..9218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(11702..11715,1..13)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 ccaccaccac caccctttgt acctggtgat gcggtgaatg ccatgtgttt atcttctaat
       61 ggaataggtt ggaataacaa agtaaaaccg acatcaatgg tttcttgatt ttgtacaccg
      121 tgaccgtcac catgcaaata catgaaatga gacattctaa ctaagttcaa caagaattcg
      181 gttgtagtac cagtcaatgg ggagtcttta tctgcggtgt aagcattaac ctttttccac
      241 atttgtctca tcatatcctt aatgtgttgt ctggcaactt cttctgatgc acctgtttca
      301 tgcatgtagc attgtataga tttaggtacg tcacctcttt gaatttcgtc actggaagta
      361 cctaagtcat cttgcaatct gaaaattttt gaagaccagt gaacgatatc tgggtttgat
      421 tccaagaatt ccaattcctt tttaatgata gggttagtac cactcaaata ggagattgtg
      481 atgatcaatg gaccagtgat tgagactaaa ccgttttcca agtattcttc caacttaggt
      541 gtgtacttag aatggtacca cttagcctct accaaatagg cttgtatcaa acccaaccaa
      601 gcgtttttaa tagataacaa taagtcgaaa tcttgttgct tcaaaacgta gtaagcgaat
      661 tcgttgacga agttgtacaa ggctaaaaag cacatcttca tgtaacctgg caaatgcttt
      721 aatgcatagt tgatatccca tctttcaaca gcgtctgtaa agatttccaa ttcatccaaa
      781 gtaccgtata cgtcatagat gtcatctata acggtgatca atgcaataga gattgtcaaa
      841 actcttctac agtaagcgaa ttgaggttca aaggcaatac ccattgacca taagaaagat
      901 gcgaccaatc tatttctagc gaatgacaac ttttcaccca aaccggtatc cttccaccaa
      961 ccagagattt ccttcaattc ttcttgatag atggcttgta atgtattgaa ttccatcttt
     1021 gccaattcca acaacaagtg gttcttatct tctcttcttt cgtatatatg gatgaaccat
     1081 cttgcttcca acataggaac tttccaatgc aatggtaatt ccaaggctct ctttgcttgt
     1141 tcagctacga aaacatcttc ttccatattt ttacttatca taacttcctt caagtgtttg
     1201 gaagtgaatt gccatgcttc ttccattata ctttcacctt ccaatgagta gtaagaagct
     1261 tcatgcaagg acaatatacc cttgaagtca tcacaaataa aaccaccttg gtcatccttg
     1321 aaaccgttaa aaacttcttg actgactggg taaccgtgtt gtcttaacaa tctgaattcc
     1381 aaggaagtag cgtacaaatt ttcctttctc cagttgtaat ccttattgtt gttatagatg
     1441 ttgttcaaga tgtttctgat ttcggtttca aatctatggg ctaaacccaa tctttgcaag
     1501 ttatcaatca attccaattg gtccaaaggt tcgatgacat ctttgatggc tatctttact
     1561 ttaccacgta attcttctgc tcttctcttg tatgcttcgt cggtgtagtt agagttcaag
     1621 gattgtaaaa agtcgtggtc ccaaatggat ggttgatagt tagctgatct tctaccagaa
     1681 cctggcttag tgattctagt ttgggcatcc ttcaaggctt cgtcaacagt ttgtctaccg
     1741 gaagcggcgt tgatgacagc agtacggacg gcataccaga aagcagacat ttgtggaata
     1801 ttaggcatga tttcaccctt ttgagcgttt tccatagtag cagcaattct tggatcttta
     1861 gccaattctt cttcgtaaga tttcaaggcg acagcaccca atggtttatc tttgttaacg
     1921 gcttccaaac cttcgtcagt taacaagtag ttttccaaaa attccttagc caattcctta
     1981 tttggagaag cagcgttgat accagcagac aaaacaccaa caaatggttt ggatggttga
     2041 cccttaaagg taggcaagac agtaacacca tagttgacct tagaagtgtc aatgttggac
     2101 caggcccaag gaccgttgat ggtcatagca gtttcacctt tgttgaaagc agcttcagcg
     2161 atggagtaat cggtgtcagc attcatatgc ttgtttttaa tcaaatcgac caagaaagtc
     2221 aaaccagctt tagcaccggc gttgtcaaca ccaacgtcct taatatcgta tttaccattt
     2281 tcgtacttaa aagcgtaacc accgtcagca gcgattaaag gccaagtgaa gtatggttct
     2341 tgtaagttga acatcaaagc ggatttacct ttagctttca attctttatc caaagctggg
     2401 atttcttccc aagtttttgg tggattaggt aacaaatcct tgttatagat caaagacaag
     2461 gcttcaacag caattgggta agcaatcaac ttaccgttgt atctgacagc atcccaagtg
     2521 aatgggtaca acttatcttg gaaagcctta tctggggtaa tttcagctaa caaaccagat
     2581 tgggcgtaac caccaaatct atcgtgagcc caaaagataa tatctggacc gtcaccagta
     2641 gcagcaactt gtgggaattt ttcttctaat ttgtctgggt gttcgacggt gactttgata
     2701 ccagtatcct tttcaaactt cttaccgact tcggctaaac cgttgtaacc tttgtcaccg
     2761 ttaatccaga taaccaactt accttcttca atttttggac ctgggttaag ttcaacgtca
     2821 ccagccaact tcaacaaaga gaagttagta gcaccaactc ttttaacgaa aacccagtaa
     2881 gagtcaccag ataaagcctt tttcatgtga actttaacct tagttggacc tctggaagtt
     2941 gggatcatcc attcaaacat agtgtttaag ttaccagaag caacaccttc cttaaactta
     3001 ccatagaact ctggagagtc agtacctgga gcaacgtcct taaagaagtt cttaccaata
     3061 acttgttttg gatctctacc agtaatatca ccttcagcag cattgtattg caaaatgtta
     3121 ccatcaccgt ccaattgaat agcaccgaaa gctaaaccgt ctaattgacc atcatccatt
     3181 ttagccaaag tgttttcaat atcttcagaa ccgaaagcaa cgtgttccat tttggatcca
     3241 ctgtaaaaac tttttttatt atactatttt ctattgttta atagtcgttg ttcttgtgga
     3301 tatatgaaca agtaatcaaa aaagttatca caaatgaact cagatatatt tcagccgaat
     3361 ggctagagtt tatatatgtc tgtctaaccc acactttttc cgcattttat tcctcaactc
     3421 atccctccct tgaacaacga ctgctaacaa cgtctcactc taaaatcttt acacgtttag
     3481 ctttacagga ggagcataat tgacttttgt tcctatttca agttgggaag ggcttacttt
     3541 agcattagct cggagtggtc caccccggaa ctatggcctg cggaaatact ctcacaggca
     3601 catggcccct gaactgcggg gagtgtgccg cacggaagag tgagcgccgg tgcctaccgg
     3661 aagaacccct tcctcagtat ttgagcacgt gactcgaatg ctggccgcaa aaaagaaaaa
     3721 agaatgtaat ccggttgagc cgccccggaa gacgcttttc cggtagttaa ttctttatac
     3781 attactggaa cagattctgc tccgtttaat tagacttgga tgcaaggttg cctgtgatag
     3841 ctttagtggg tctattctac ggccttctaa tggcaccgtt atgacctgga tctggtacgt
     3901 ctgtacgatt agcggggcaa gttcaaataa aattggttta ctcgagttaa tttatctcat
     3961 gaagtaatga tacctcagcg atgtgctcgc tcgttagtat gcttcataaa ttcattatta
     4021 tttttttttg gaagtgtttt tcgacaaaag ttgcattttt acgaaggata tgtaaactca
     4081 agaagtacta aataaatata aatacaattc gaaaatttga aaaaagtagg agacaacact
     4141 ttccttcctc acgaagaagt taagcttgag catcggaccg aagcataaga atgcagcaaa
     4201 ttgataatat tcccgttaag ccagtcgtag gaaaaggaaa agaatgagta gcaaatgtca
     4261 cacaacaaac gcgcaccatc cctacataca caaacacaca cacacacaca catatatata
     4321 tattctaccc catataagcg agtgattcat catgaaaaga atttatcact tcgaatttaa
     4381 ataataacat tcttacctga tgtacaactc tttactctca tcttgcgaaa agaaaagggc
     4441 agggtgccgc tgaaacagcg gcgttgctga tgaaaataac cctgtgtttt cgcctcattc
     4501 tctcaaaacc ttcacagatc cttttcttgt tatagcctca ataaaatcac agtacactaa
     4561 cacgtaatta taaatagtaa ccggaaccct cgctgcaata cgcaaattgg tacctacttt
     4621 gtaaggcaca ggaaaccccg tgcactctgc cactacactt tatttacgct taatcatgaa
     4681 tttaacatgt tatgaaagat ttattgtata tttaaagaaa aatttaaaaa taatattaaa
     4741 tttattaatt aaaccaatta gagctagctt tgggcccgca ggctaaccgg aacctgtatt
     4801 atttagttta tgctacgtta aataaagacc tttcgttcac ataactgaat gtgtaatggc
     4861 cttgagattt caagcatacc aagttggtgg agacggggtc gttacaaaag actctttaag
     4921 ccaagatttc cttgacagcc ttggcgatag catcgccaac ctcagtggta gagttagaac
     4981 caccaaggtc accggttctg acacctgcat ccaagacatt tctaacagct tcttcaagag
     5041 ccctaccttc ttcaaccaaa tccaaggata acttcaacat catagctgca gataagatgg
     5101 tagcaattgg gttaaccttg tttgctggta aatctggggc agaaccatga catggttcgt
     5161 acaaaccgaa tgccttgtta gtgtcaggta gggaagctag agatgcagaa ggtaataaac
     5221 ccaaagaacc tggaataaca gaggcttcat cggagataat atcaccaaac atgttgttgg
     5281 taataacaac accgtttagc ttagttggtg atttaaccaa aatcatagca gcagagtcga
     5341 tcaattggtg ctgaacagtt aattgtggga actcagtctt gatggtttct tcaacagtct
     5401 ttctccacaa tctggaagag gcaagcacgt tagccttgtc aagagaccag attggtaatg
     5461 gtgggttttg ttgcaatgcc aagaaagcag ccattcttgt aattctttga acttcaggaa
     5521 cactgtattt ctcagagtcc caagcaactc cgtcaccttc atcttctttt ctttcaccaa
     5581 agtagatacc accaaccaat tctctaacga cgacgaaatc ggtacccttt gcatattcag
     5641 gcttcaaagg agaaagatct agtaaagaat cagaagcaaa gttacatggt ctcaagttgg
     5701 cgtatagacc caattccttt ctgatcttca atagaccttg ttctggtcta actgcgcccg
     5761 taccccattt tggaccacca acagcaccta gtaagacagc atcggctttc ttagaggctt
     5821 ctagagcttc atctggtaaa ggagtgccag tggcatcgat ggcagcaccc ccgatcaagt
     5881 gatgttggaa attgaactta atttctggac ggacttcagc aatggcattc aagaccttaa
     5941 tagcttcgtc agtaacttct ttaccgacgt gatcacccgg taggacaacg atattcttag
     6001 acattttgcg gccgctttat cttattgatc ttctttgttt agccttttca acagcagaag
     6061 aaaagcatat gagatacatt taaaattgtt ttaaaataat tttaaaagat aatatctaaa
     6121 agattgtatg catacgaacg aagttgcatt gggaagcccc aacgcagtga ggcaagccga
     6181 aggtagaaag aaaatcccct tgtgcgtcca gtgagaaaca gcaagcagtg ggtaggaagg
     6241 cccagtttag cctgactgtt ccaacggggc tcccaggctg aggaaatgct tctctccgta
     6301 atgggagagc ctaactccag agcgtggact tcggctcacg gttttctctt tttttggcgc
     6361 acaatgactg ctgagacaaa aaaagaaagc aactccatgt aaacacccgt acatattaaa
     6421 taagtgaccc atgcctcctc atctcttcat tgcaaagtac ggaaaccgtt agattggtac
     6481 caacaaggtt gctatatcgg gcatacaaga taagaaactt aaagaacgcc gagacaagaa
     6541 tatgtaatga taagcctcct ccggggtgtt agactgatta gtacatgttt aaactgaatt
     6601 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6661 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6721 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     6781 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     6841 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6901 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     6961 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     7021 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7081 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7141 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7201 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7261 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7321 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7381 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7441 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7501 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7561 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7621 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7681 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7741 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7801 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7861 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7921 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7981 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8041 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8101 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8161 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8221 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8281 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8341 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8401 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8461 tggcttctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8521 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8581 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8641 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8701 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8761 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8821 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     8881 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     8941 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9001 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9061 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgttt
     9241 aaactatgtt ccttgatacc tcgacgtgga gcacgaaata gaagaaaaaa gtataatgag
     9301 gatggaataa ttttcttgaa tctgagcaga aaaacgaacg aacactctaa tggatttgtt
     9361 aatggctgtt ggtagtaaaa agaacaactt ccacagcatc aacgcttcta tcaaaacgat
     9421 tgctaataaa ctgttgagcc cgataattac gttgctcacc atatcttaat caagtcctta
     9481 atgcactatt cttgtcgtcg gatagtttat ttacgtgtgg ttttcgtatc tcatcaatat
     9541 caactctact agttcccatc tatcttcctc tcaattttct cgtgctatta atattgcagt
     9601 tcacatacca gatggagcca ttggcacaaa tgtggtttcc tttcttattg ctgtatccaa
     9661 cttttgtgaa gctacaggtg cgacaaacat aaaaaagttc ttgccgttaa gaagctgaag
     9721 ttgcttctcg ctataccaga ggggattatt gtcgggagtc atgatgcgac atttacccgg
     9781 ttgagtaagg aaggggtgga ggaatcagaa gagagataaa caaacataca cgtcaaaaca
     9841 gtatagttca agtaacacat atagacatgt acacgcgcga ttacgtgtgt acgtgcacat
     9901 gttaatgtta tatacatata tagatataga tatataattg caataatgaa ctacaaatta
     9961 ttataccccc gttctttttt ttttcttttt tttagtaatt tttctttaga tttatcggaa
    10021 tacttacttt ggtgcttcac cttcggcggc agcaggtggc tgctgttgct gatgttgttg
    10081 ttgttgttgt tggtcttcag cttgaccgga ctcggacatg tctgaagtcc ataaggttaa
    10141 attgtccctt agcagttgca tgataagtgt gctatctttg tatgattctt cagacagagt
    10201 gtccaactca gcaatagcgt cgtcaaaagc ttgcttggcc aaatggcagg ctttgtctgg
    10261 agagttttga atttcgcatg caaatcatta cgaccgagat tcccgggtca cgccacagaa
    10321 acctcagaat aagctaaagg gtaatataat aaagcccttc gattcaataa tagcattacc
    10381 caagaggcat agcggcaaac taagttgaca acacaaaatc aaatcgcttc atttttattt
    10441 tattaatgat attttattag agaggaaatt atgtaatatc tagatattaa atataaagta
    10501 gatctctgtc taaaatattc ttaacaaaat cttatattcg aaaggatgac ggtccaatca
    10561 aaggattgta taagagaaat tagttttcga aatcaatttt tacttgacct gaacttaacg
    10621 atttgctctc aatccgccca tggttactat ttacttctct tgtaaacctt gttcaagaac
    10681 gcagttaaga catcagcttt gaagccacga gactcatcga cctgagaaat tttggccttc
    10741 aaatccttgg caatagactc ttcatattcg tggtatagct gttcaatttt caagtcattg
    10801 aaaatctttt tgcatttggc ttctgcgact gagtccttct taccgtaatt ttcgtctaaa
    10861 gtctttcttt gttctgcgga agcaagttct aatgccttgt tgattaccca agaacatttg
    10921 ttatcttgga tatctgtacc gatcttaccg atctgttctg gggtaccgaa gcagtctaag
    10981 tagtcatctt gaatttggaa gtattcaccc aatggaatca agacatctct ggcttgtttc
    11041 aaatcctttt catccgtgat accggcaacg tacatggcca atgcgacagg caagtagaaa
    11101 gaatagtaag cagtcttgaa agtaactatg aaggagtgct tctttaggga gaacttactc
    11161 aagtcgactt tgtcttcagg tgcagtgatt aagtccatca attggcccaa ttcggtttgg
    11221 aaggtgacct catggaacaa ttcggtgata tctatgtagt atttttcgtt tctgaagtga
    11281 gatttcaaaa gcttgtagat agcagcctct aacatgaatg cgtcccagat ggcaatttcc
    11341 ccaacttcag gaaccttgta ccaacatggt tggcctcttc tggtaatgga cttgtccatc
    11401 atatcatcgg cgaccaacca gtaagcctgc aacaactcaa tgcaccaacc tagaatggca
    11461 accttttcgt attcttcttg ccccaattgt tcaacggtct tgttggagag aatagcatac
    11521 gtgtccacaa cggacaaacc tctatttagc ttaccgcctg gagtgttgta gttcaatgag
    11581 tgggcatacc agtcacatgc ttccttaggc ataccgtaag ccaaaagcga tgcgttcaat
    11641 tcctctacta atttagggaa aacgttcaag aatctctctc tcctaatttc tttttctgaa
    11701 gcaccaccac cacca
//
