LOCUS       sequence-370850-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmids for Long Stokes shift red fluorescent 
            protein LSSmApple.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Ejike JO, Sadoine M, Shen Y, Ishikawa Y, Sunal E, Hansch S, Hamacher
            AB, Frommer WB, Wudick MM, Campbell RE, Kleist TJ
  TITLE     A Monochromatically Excitable Green-Red Dual-Fluorophore Fusion 
            Incorporating a New Large Stokes Shift Fluorescent Protein.
  JOURNAL   Biochemistry. 2024 Jan 2;63(1):171-180. doi: 
            10.1021/acs.biochem.3c00451. Epub 2023 Dec 19.
  PUBMED    38113455
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.3c00451"; journalName: "Biochemistry"; date: 
            "2024-01-2- 2"; volume: "63"; issue: "1"; pages: "171-180"
COMMENT     Sequence Label: pBAD-LSSmApple
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..708
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEX
                     FQTAKLKVTKGGPLPFTWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMIF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGLEACEERMYPEDGALK
                     SEYKEWLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agncctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcacctggg acatcctgtc ccctcagttc atgtacggct ccaaggtcta cattaagcac
      241 ccagccgaca tccccgacta cttcaagctg tccttccccg agggcttcag gtgggagcgc
      301 gtgatgatct tcgaggacgg cggcattatt cacgttaacc aggactcctc cctgcaggac
      361 ggcgtgttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtt
      421 atgcagaaga agaccatggg ccttgaggcc tgtgaggagc ggatgtaccc cgaggacggc
      481 gcccttaaga gcgagtataa ggagtggctg aagctgaagg acggcggcca ctacgccgcc
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgccaggcgc ctacatcgtc
      601 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaag
//
