LOCUS       sequence-370851-         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmids for pH sensitive fluorescent protein 
            Lime.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Shen Y, Wen Y, Sposini S, Vishwanath AA, Abdelfattah AS, Schreiter 
            ER, Lemieux MJ, de Juan-Sanz J, Perrais D, Campbell RE
  TITLE     Rational Engineering of an Improved Genetically Encoded pH Sensor 
            Based on Superecliptic pHluorin.
  JOURNAL   ACS Sens. 2023 Aug 25;8(8):3014-3022. doi: 
            10.1021/acssensors.3c00484. Epub 2023 Jul 23.
  PUBMED    37481776
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.3c00484"; journalName: "ACS Sens"; date: 
            "2023-08-25- 25"; volume: "8"; issue: "8"; pages: "3014-3022"
COMMENT     Sequence Label: pBAD-Lime
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggtgagta aaggagaaga acttttcact ggagtggtgc ctatcctggt ggaactggat
       61 ggcgacgtga acggccacaa gttcagcgtt agaggcgaag gcgaagggga tgccacaaac
      121 ggcaagctga ccctgaagtt catcagcacc acaggcaagc tgcccgtgcc ttggcctaca
      181 ctggtcacaa cactgaccta cggcgtgcag agcttcagca gataccccga ccacatgaag
      241 cggcacgatt tcttcaagag cgccatgcca gagggctacg tgcaagagag aaccatcagc
      301 ttcaaggacg acggcaccta caagaccaga gccgaagtga agttcgaggg cgacaccctg
      361 gtcaaccgga tcgagctgaa gggcatcgac ttcaaagagg acggcaacat cctgggccac
      421 aaacttgagt acaacttcaa cgaccaccag gtctacatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcaa gatccggcac aacgtggaag atggcagcgt gcagctggcc
      541 gatcactacc agcagaacac acccatcgga gatggccctg tgctgctgcc cgataaccac
      601 tacctgttca ccaccagcac actgagcaag gaccccaacg agaagcggga ccacatggtg
      661 ctgctggaat ttgtgaccgc cgccggcatt acacatggca tggatgaact atacaaatga
//
