LOCUS       sequence-370860-       14301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of lycopene synthetic module at 
            RPL25 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14301)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 14301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pILAC2
FEATURES             Location/Qualifiers
     source          1..14301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1100..1116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1100..1116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1113..1135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1124..1140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1148..1154,1155..1172,1173..1178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1193..1214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1331..1348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1502..2090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1582..1601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2261..3052,3053..3121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2884..2903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3122..3226)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3294..3312
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3350..3372)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3472..3491
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3685..3707
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3699..3716
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3700..3716
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(9169..9416)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     9382..9400
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(13649..14090)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(13655..13678)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    13973..14090
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ataaggatcc cccgggaatc tcggtcgtaa tgatttgcat gcgaaattca aaactctcca
       61 gacaaagcct gccatttggc caagcaagct tttgacgacg ctattgctga gttggacact
      121 ctgtctgaag aatcatacaa agatagcaca cttatcatgc aactgctaag ggacaattta
      181 accttatgga cttcagacat gtccgagtcc ggtcaagctg aagaccaaca acaacaacaa
      241 caacatcagc aacagcagcc acctgctgcc gccgaaggtg aagcaccaaa gtaagtattc
      301 cgataaatct aaagaaaaat tactaaaaaa aagaaaaaaa aaagaacggg ggtataataa
      361 tttgtagttc attattgcaa ttatatatct atatctatat atgtatataa cattaacatg
      421 tgcacgtaca cacgtaatcg cgcgtgtaca tgtctatatg tgttacttga actatactgt
      481 tttgacgtgt atgtttgttt atctctcttc tgattcctcc accccttcct tactcaaccg
      541 ggtaaatgtc gcatcatgac tcccgacaat aatcccctct ggtatagcga gaagcaactt
      601 cagcttctta acggcaagaa cttttttatg tttgtcgcac ctgtagcttc acaaaagttg
      661 gatacagcaa taagaaagga aaccacattt gtgccaatgg ctccatctgg tatgtgaact
      721 gcaatattaa tagcacgaga aaattgagag gaagatagat gggaactagt agagttgata
      781 ttgatgagat acgaaaacca cacgtaaata aactatccga cgacaagaat agtgcattaa
      841 ggacttgatt aagatatggt gagcaacgta attatcgggc tcaacagttt attagcaatc
      901 gttttgatag aagcgttgat gctgtggaag ttgttctttt tactaccaac agccattaac
      961 aaatccatta gagtgttcgt tcgtttttct gctcagattc aagaaaatta ttccatcctc
     1021 attatacttt tttcttctat ttcgtgctcc acgtcgaggt atcaaggaac atagtttaaa
     1081 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1141 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1201 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1261 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1321 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1381 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1441 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1501 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1561 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1621 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1681 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1741 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1801 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1861 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1921 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     1981 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2041 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2101 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2161 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2221 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2281 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2341 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2401 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2461 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2521 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2581 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2641 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2701 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2761 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2821 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2881 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2941 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3001 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3061 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3121 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3181 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3241 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3301 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     3361 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     3421 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     3481 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     3541 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     3601 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     3661 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     3721 tcagtttaaa catgtactaa tcagtctaac accccggagg aggcttatca ttacatattc
     3781 ttgtctcggc gttctttaag tttcttatct tgtatgcccg atatagcaac cttgttggta
     3841 ccaatctaac ggtttccgta ctttgcaatg aagagatgag gaggcatggg tcacttattt
     3901 aatatgtacg ggtgtttaca tggagttgct ttcttttttt gtctcagcag tcattgtgcg
     3961 ccaaaaaaag agaaaaccgt gagccgaagt ccacgctctg gagttaggct ctcccattac
     4021 ggagagaagc atttcctcag cctgggagcc ccgttggaac agtcaggcta aactgggcct
     4081 tcctacccac tgcttgctgt ttctcactgg acgcacaagg ggattttctt tctaccttcg
     4141 gcttgcctca ctgcgttggg gcttcccaat gcaacttcgt tcgtatgcat acaatctttt
     4201 agatattatc ttttaaaatt attttaaaac aattttaaat gtatctcata tgcttttctt
     4261 ctgctgttga aaaggctaaa caaagaagat caataagata aagcggccgc aaaatgtcta
     4321 agaatatcgt tgtcctaccg ggtgatcacg tcggtaaaga agttactgac gaagctatta
     4381 aggtcttgaa tgccattgct gaagtccgtc cagaaattaa gttcaatttc caacatcact
     4441 tgatcggggg tgctgccatc gatgccactg gcactccttt accagatgaa gctctagaag
     4501 cctctaagaa agccgatgct gtcttactag gtgctgttgg tggtccaaaa tggggtacgg
     4561 gcgcagttag accagaacaa ggtctattga agatcagaaa ggaattgggt ctatacgcca
     4621 acttgagacc atgtaacttt gcttctgatt ctttactaga tctttctcct ttgaagcctg
     4681 aatatgcaaa gggtaccgat ttcgtcgtcg ttagagaatt ggttggtggt atctactttg
     4741 gtgaaagaaa agaagatgaa ggtgacggag ttgcttggga ctctgagaaa tacagtgttc
     4801 ctgaagttca aagaattaca agaatggctg ctttcttggc attgcaacaa aacccaccat
     4861 taccaatctg gtctcttgac aaggctaacg tgcttgcctc ttccagattg tggagaaaga
     4921 ctgttgaaga aaccatcaag actgagttcc cacaattaac tgttcagcac caattgatcg
     4981 actctgctgc tatgattttg gttaaatcac caactaagct aaacggtgtt gttattacca
     5041 acaacatgtt tggtgatatt atctccgatg aagcctctgt tattccaggt tctttgggtt
     5101 tattaccttc tgcatctcta gcttccctac ctgacactaa caaggcattc ggtttgtacg
     5161 aaccatgtca tggttctgcc ccagatttac cagcaaacaa ggttaaccca attgctacca
     5221 tcttatctgc agctatgatg ttgaagttat ccttggattt ggttgaagaa ggtagggctc
     5281 ttgaagaagc tgttagaaat gtcttggatg caggtgtcag aaccggtgac cttggtggtt
     5341 ctaactctac cactgaggtt ggcgatgcta tcgccaaggc tgtcaaggaa atcttggctt
     5401 aaagagtctt ttgtaacgac cccgtctcca ccaacttggt atgcttgaaa tctcaaggcc
     5461 attacacatt cagttatgtg aacgaaaggt ctttatttaa cgtagcataa actaaataat
     5521 acaggttccg gttagcctgc gggcccaaag ctagctctaa ttggtttaat taataaattt
     5581 aatattattt ttaaattttt ctttaaatat acaataaatc tttcataaca tgttaaattc
     5641 atgattaagc gtaaataaag tgtagtggca gagtgcacgg ggtttcctgt gccttacaaa
     5701 gtaggtacca atttgcgtat tgcagcgagg gttccggtta ctatttataa ttacgtgtta
     5761 gtgtactgtg attttattga ggctataaca agaaaaggat ctgtgaaggt tttgagagaa
     5821 tgaggcgaaa acacagggtt attttcatca gcaacgccgc tgtttcagcg gcaccctgcc
     5881 cttttctttt cgcaagatga gagtaaagag ttgtacatca ggtaagaatg ttattattta
     5941 aattcgaagt gataaattct tttcatgatg aatcactcgc ttatatgggg tagaatatat
     6001 atatatgtgt gtgtgtgtgt gtgtttgtgt atgtagggat ggtgcgcgtt tgttgtgtga
     6061 catttgctac tcattctttt ccttttccta cgactggctt aacgggaata ttatcaattt
     6121 gctgcattct tatgcttcgg tccgatgctc aagcttaact tcttcgtgag gaaggaaagt
     6181 gttgtctcct acttttttca aattttcgaa ttgtatttat atttatttag tacttcttga
     6241 gtttacatat ccttcgtaaa aatgcaactt ttgtcgaaaa acacttccaa aaaaaaataa
     6301 taatgaattt atgaagcata ctaacgagcg agcacatcgc tgaggtatca ttacttcatg
     6361 agataaatta actcgaggcc gcacgccaca gaaacctcag aataagctaa agggtaatat
     6421 aataaagccc ttcgattcaa taatagcatt acccaagagg catagcggca aactaagttg
     6481 acaacacaaa atcaaatcgc ttcattttta ttttattaat gatattttat tagagaggaa
     6541 attatgtaat atctagatat taaatataaa gtagatctct gtctaaaata ttcttaacaa
     6601 aatcttatat tcgaaaggat gacggtccaa tcaaaggatt gtataagaga aattagtttt
     6661 cgaaatcaat ttttacttga cctgaactta acgatttgct ctcaatccgc ttatcagaaa
     6721 gcaagaacac caacggatcg agcgatcacg gccccaatca acaacaccaa aaggtatacc
     6781 catctctcca aatccatgaa cttcagctgg acctgagaat cgatacccgt tccatttgat
     6841 ttgagaggtt ttccatatgg tacggagagt gacatattgg gatctggagc tggggatcgg
     6901 tcaaaggatt cgagaacttg gttggcagtt aacttggctc cagccaagac gatgggaacc
     6961 ccagttccgg gatgagtcga agccccgaca aagaacaact tgtcatactt gggatgtctg
     7021 gtgctcggcc tgaaaccaag aacttgcata aagttgtgag ccagtcccaa gatgcttccg
     7081 tccttgaggt taaattcttt ctcccaagag ggagcatcgt gaaccttctc tgccacaatc
     7141 attttttcaa agtcgggaag tccaagcttg gcggaaagcg tttggatcac aaacttcctt
     7201 gcccgagcaa caagcttgtt gtaatcttga gggttcgaag cgtcgatatg gccacacggc
     7261 acaagaatga cgatagcatc tttgccttcg ggagcggcag aaggatcgat tcgcgaggga
     7321 acgttcacgt aaaaggaagg atcggctggg agacccaact cctcgaagat tgtgtcgaat
     7381 gatcccttga agtcctcggc caagaagata ttgtgtccgc ccagaccgtc cacgattcgg
     7441 tccatgctcc agtagaagct caaactactg caacttccct tgagcttctt tccaccaact
     7501 aagtcagccc accaacttct cttgacttca cccagttggc caatcttgtt tctggcatcg
     7561 tcaggaatca agtgctcgga ggcgtaaacg aggtcagcat tgacaatcac aacatcggcg
     7621 tgatgttcct cgccggattc aagtcgaaca ccagtcgctc ggtccttggc aggagagaga
     7681 agaacctggg aaactggagc gttgaaattg aacttggctg agggattgtt gcgcttgacg
     7741 atctgaagaa gagtattagg aacctgccaa aagcctcctc tcggatacca gatgccctcg
     7801 gtcaattcgg tgtattggag caaggaatat gttccgggcg cactgtatgg gctttgaccc
     7861 atgtacatca ctgcaaacga gaagactctt cgtaatctgt cggtcttgaa atatcgacaa
     7921 actcttgtcc agatagactc gaaggggtga agagccagga tttggccaat gaactgtagc
     7981 cgtaagaatg ctgcgaagcc agggaagttc ttctgcagga cgtgaacgac agcaagctcg
     8041 taatgtctgt gggcttcttg gataaacgac aagaaccgat caaatccatc tttgccttca
     8101 aaacgctcga cttcccgctt gagcaacgcc atgtcggttg aaaaagtgaa agtctcttca
     8161 tcgtggaagt ggcaaacata gttgggttca cacttgatga gatcgaccca atcttccatc
     8221 ttctccccca aatcttcgaa tgtctgcttg aagagatctg gcaagagcag caaactgggc
     8281 ccctgatcga atcgataacc atctcgctcg attaaagagc atcgacctcc ggagtagtcg
     8341 ttcttctcga acaccgtgac ctggaaacct tctttagcaa gacgagcggc agtggcgatt
     8401 ccaccgatac cacatcccac gatgatagct gtgggtttat cctgatcttg ttcttttccc
     8461 attgtaaatg tgtgtatata ttatattata gtattgttga tattatagtt cttattgata
     8521 gatgagggag atgtagatgg aaagagaaga agtggtttct agcttatatt cagccgaatg
     8581 gctagagttt atatatgtct gcaagccccg taattcctcc acattttact ccgcatcaca
     8641 tccctcctca gaacaatagt tgcctggtgc gttgcgcatg tttgaaagtg tacaaacact
     8701 taaccttcca gtaggagctt aatccacatt atttattttt tagcgtagag aatggcttaa
     8761 ctaacctcgg ctcggagtgg tcctctccga ctcggtgccc tgcagcgata tcatcacagg
     8821 cacgcaggcc cctgaacggc acagaccacg ccgcacggac gagtgaatgc cggtgcctgc
     8881 tggtgccgcc ccttccctgt tttctgggcg ggaagtgctc agggggtgat ttcggatgca
     8941 cgtgacctcg aggcgacttt ttggcgacaa aaggactata atcatatatt cggccgtcca
     9001 aggcccgatc cttaatgtgt tattgcagga tatatgctgc aattcattgg agttaggtat
     9061 gaatctgctg gctaaaaatc gggtgtgcgg ctgcttggag tttttcagcc tgtgttccgg
     9121 cattttggtg atctggatct tgtaatctcc tgttctctgt gggctagcgc aaattaaagc
     9181 cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt ataatgttac atgcgtacac
     9241 gcgtctgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat
     9301 aactataaaa aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt
     9361 tagagcggat gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg
     9421 aggtcgactt actgcccttc ccatccgctc atgaccacac tcaagacttt ccgtactctc
     9481 ctccatccgg caactgtcct tctctctccg acgtctcctt tccaaacgac tttgatctct
     9541 cggccgatca gtaggtagct cgcgcaagcc gctcgcattc ccgcttgaac ctcggtagga
     9601 agtcggtcaa ttcccttata agaatgtttg gcaagatcct ctgcgtaggc gactaatgga
     9661 agcgagtacg tcttccattc gaaccggaag ctttctgagg cgtttgaaga tggtaatgtg
     9721 gacgaaggag atagactgag gagtttgtcg aaatcttgag gccgaggttc cgtccaatca
     9781 gtcgggatcg caagctttga ttcatcccga agaccaaaga atgagagtgg taggtaaaat
     9841 ctcccttctg ttgcgtcccc tttaatgtcc ctagcaatgt tcaccaactg aagggcagtt
     9901 cccatctctc ggcttgccac taacacagct tctctttctt ctatggtggc agggacctga
     9961 cttggtgcac ttgcccaaga gacatagacc aatagctcgg cgactgagcc tgctacacat
    10021 agaccatagt ccagcaagtc agctgtggtc tcgataggcg tcttccgagc ctggactgcc
    10081 tctgtcgata agggaaagat aagatcagtg gtgtatcctc taaggagttc gtcgagtggg
    10141 tatcgaggga tcagcccttg caacttagcg agcaacctga aggcgaaatg gtattgaacg
    10201 ggaacccttt cggtaaggaa ttgaacgagc tcggcaggcg agagcgaagg aggaggcggg
    10261 aggggataca ttcccgtggg tcgggaaggg tgcgaaggag gaagtaaagg cgaagaaagg
    10321 atcttgtcag gttgcgaagg gtgtagcggg ggcccaaata gtagggtaag aaaatcggag
    10381 accatgtcaa ttgtggcatg cgggttggaa gatacttcag gagagtcgat aagatcatca
    10441 gtcacccggc agaatgcgta tagtccaacc agcctctccc taacttcgct aggaaatcca
    10501 gccgaggcaa caaaaaagct ccggctcttt ttctccaaca acttgactgc cagttccaag
    10561 tcacgttttg gctgagaaga gtatggtcgg ctgctaaaaa acagggagag cacaggcggt
    10621 gtaatgaggg gaaatgaaga tggcatcttt ttgttgccat aaatagttcg accgtgtagc
    10681 aggtataggg cctgagtatg atcgcaggca gacagaccca gaacaatcat tagattcgtc
    10741 agtaagaaga acatagcttc ctcaatgggt agtacacctc caagcctcca ccctacaatc
    10801 ttctcatcgt tgatcgacca agagtcttga ccgacagcaa cataatctac ccaaatcaga
    10861 tacaccgtcg ggatcatgat tgttgcaata gttgactttg ctcggccact tttccaatcg
    10921 aaagcatatt cgcctgataa tgctgccaag agcatggtag gtggggtgat gagtaaggag
    10981 agtgcccgca tgtagaagta gtgatctgtc acgagcgggt cgggcgatgg gctggggtga
    11041 gcggtaaata ggtagataat gggcagaggg atgagcgcct tgagcgcgag agaaagggcg
    11101 gacgatctag tcttgggaag cgcgagagat gggagaaggt gcctagttgc caagacgtag
    11161 accaagccgg tgattacggt ttgaatgaca aagaaagcgt acttttcata tggaacatct
    11221 agaaacgttc caaacacgcc ttggccactc tccgctgatg gatatgtcca tgcgccattt
    11281 ctgatgatcc atgagtccca tggtgtggtt gcactaaacg caataaatac gaggatcgat
    11341 attttgtaga tgtcaaattt tgtcaaaatc ggggaagtga gcaggccgag aagaccaaga
    11401 attgggagag tatagatcag atggatctgg taatatgcga gagccgtcat tgtaaaaaac
    11461 tttttttatt atactatttt ctattgttta atagtcgttg ttcttgtgga tatatgaaca
    11521 agtaatcaaa aaagttatca caaatgaact cagatatatt tcagccgaat ggctagagtt
    11581 tatatatgtc tgtctaaccc acactttttc cgcattttat tcctcaactc atccctccct
    11641 tgaacaacga ctgctaacaa cgtctcactc taaaatcttt acacgtttag ctttacagga
    11701 ggagcataat tgacttttgt tcctatttca agttgggaag ggcttacttt agcattagct
    11761 cggagtggtc caccccggaa ctatggcctg cggaaatact ctcacaggca catggcccct
    11821 gaactgcggg gagtgtgccg cacggaagag tgagcgccgg tgcctaccgg aagaacccct
    11881 tcctcagtat ttgagcacgt gactcgaatg ctggccgcaa aaaagaaaaa agaatgtaat
    11941 ccggttgagc cgccccggaa gacgcttttc cggtagttaa ttctttatac attactggaa
    12001 cagattctgc tccgtttaat tagacttgga tgcaaggttg cctgtgatag ctttagtggg
    12061 tctattctac ggccttctaa tggcaccgtt atgacctgga tctggtacgt ctgtacgatt
    12121 agcggggcaa gttcaaataa aattggttta actagtgcgt tcaaaccaga ttttgtatct
    12181 gatccaaaga aaaaacagat gaaactggag gaaatcaaga aaaaaacaaa agaggccaaa
    12241 agaagagcaa tgaaaaaaaa ataaaagctc actgaaaaaa aaaagagata ttgttactat
    12301 tattattatt actgtcatta atactatact aataaagaaa aggaaaatac gcataataat
    12361 atttaattaa caataatata acatatatat atatatatat atatgtatgt actaccatgt
    12421 aatttcaaag actttcaaaa gaagttatac gtcaattctc gtgtacaaat cagtcaaact
    12481 gtaattcatt cgtactgata agccaaattt ctacctagta ttcatatgaa gatttgaaaa
    12541 actatgataa aaaacgaaca atatcattac tatttgcttc tcttgtaaac tttgttcaaa
    12601 aacgcagtta agacgtcggc tttgaagcca cgagactcgt cgacttggga gatcttggcc
    12661 ttcaaatcct tggcaacaga ctcttcatat tcgtggtata actggtcgat tttcaagtca
    12721 ttgaaaatct ttttgcattt ggcttctgcg actgagtcct tcttaccgta attttcgtct
    12781 aaagtctttc tttgttctgc ggaagcaagt tctaatgcct tgttgattac ccaagaacat
    12841 ttgttatctt ggatatctgt accgatctta ccgatctgtt ctggggtacc gaagcagtct
    12901 aagtagtcat cttgaatttg gaaatattca cccaatggaa tcaagacatc tctggcttgt
    12961 ttcaaatcct tttcatctgt gataccggca acgtacatag ccaatgcgac aggcaagtag
    13021 aaagaatagt aagcagtctt gaaagtaact atgaaggagt gcttctttag ggagaactta
    13081 ctcaagtcga ctttgtcttc aggtgcagtg attaagtcca tcaattggcc caattcggtt
    13141 tggaaggtga cttcatggaa caattcggtg atatctatgt agtatttttc gtttctgaag
    13201 tgagatttca aaagcttgta gatagcagcc tctaacatga atgcgtcatt gatggcaatt
    13261 tccccaactt caggaacctt gtaccaacat ggttggcctc ttctggtaat ggacttgtcc
    13321 atcatatcat cggcgaccaa acagtaagcc tgcaacaact caatgcacca acctagaata
    13381 gcaacctttt cgtattcttc ttgccccaat tgttcaacgg tcttgttgga gagaatagca
    13441 tacgtgtcca caacggacaa acctctattt aacttaccgc ctggagtgtt gtagttcaat
    13501 gagtgggcat accagtcaca tgcttcctta ggcataccgt aagccaaaag cgatgcgttc
    13561 aattcctcta ctaatttagg gaaaacgttc aagaatctct ctctcctaat ttctttttct
    13621 gaagccattt tgctagctat agttttttct ccttgacgtt aaagtataga ggtatattaa
    13681 caattttttg ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt
    13741 gaaagtatta gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa
    13801 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat
    13861 tatcctatgg ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc
    13921 aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag
    13981 tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagaccag gacgcacgga
    14041 ggagagtctt ccgtcggagg gctgtcgccc gctcggcggc ttctaatccg tacttcaata
    14101 tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa
    14161 gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg gatatgtata
    14221 tggtggtatt gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt
    14281 aagaattttt gaaaattcaa t
//
