LOCUS       sequence-370861-       13966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of lycopene synthetic module at 
            RPL25 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13966)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 13966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pILAC3
FEATURES             Location/Qualifiers
     source          1..13966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(715..731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(715..731)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(728..750)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    739..755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(763..769,770..787,788..793))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    808..829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(946..963)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1117..1705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1197..1216)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1876..2667,2668..2736))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2499..2518
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2737..2841)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2909..2927
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2965..2987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3087..3106
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3300..3322
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3314..3331
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3315..3331
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8781..9028)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     8994..9012
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(13261..13702)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(13267..13290)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    13585..13702
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 actcactcca taatattaca tatagatata ggacaagccc gcattttcat actgaaaggt
       61 aaacttctat tattatagtg gtatccaacg ttcaccgctt ccagcatagc agaaattacg
      121 tgtttttgca tatgttatgc tgatcattgt atgcttacta ccatttttct ttgcttcgcc
      181 ttgccttctt tgacgttttt ttgaagcaaa aaaaaagtca agacagatgt gcttacaaaa
      241 ccatgtaagg ctcattttca aagaagctac taatagaaag agaacaaagc gtttacgagt
      301 ctggaaaatc aatggctcca tctggtatgt gaactgcaat attaatagca cgagaaaatt
      361 gagaggaaga tagatgggaa ctagtagagt tgatattgat gagatacgaa aaccacacgt
      421 aaataaacta tccgacgaca agaatagtgc attaaggact tgattaagat atggtgagca
      481 acgtaattat cgggctcaac agtttattag caatcgtttt gatagaagcg ttgatgctgt
      541 ggaagttgtt ctttttacta ccaacagcca ttaacaaatc cattagagtg ttcgttcgtt
      601 tttctgctca gattcaagaa aattattcca tcctcattat acttttttct tctatttcgt
      661 gctccacgtc gaggtatcaa ggaacatagt ttaaacaagc ttggcgtaat catggtcata
      721 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      781 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      841 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      901 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      961 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1021 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1081 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1141 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1201 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1261 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1321 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1381 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1441 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1501 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1561 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1741 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1801 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1861 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1921 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     1981 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2041 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2101 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2161 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2221 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2281 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2341 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2401 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2461 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2521 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2581 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2641 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2701 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2761 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2821 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2881 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     2941 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3001 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3061 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     3121 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     3181 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     3241 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     3301 ccagtcacga cgttgtaaaa cgacggccag tgaattcagt ttaaacatgt actaatcagt
     3361 ctaacacccc ggaggaggct tatcattaca tattcttgtc tcggcgttct ttaagtttct
     3421 tatcttgtat gcccgatata gcaaccttgt tggtaccaat ctaacggttt ccgtactttg
     3481 caatgaagag atgaggaggc atgggtcact tatttaatat gtacgggtgt ttacatggag
     3541 ttgctttctt tttttgtctc agcagtcatt gtgcgccaaa aaaagagaaa accgtgagcc
     3601 gaagtccacg ctctggagtt aggctctccc attacggaga gaagcatttc ctcagcctgg
     3661 gagccccgtt ggaacagtca ggctaaactg ggccttccta cccactgctt gctgtttctc
     3721 actggacgca caaggggatt ttctttctac cttcggcttg cctcactgcg ttggggcttc
     3781 ccaatgcaac ttcgttcgta tgcatacaat cttttagata ttatctttta aaattatttt
     3841 aaaacaattt taaatgtatc tcatatgctt ttcttctgct gttgaaaagg ctaaacaaag
     3901 aagatcaata agataaagcg gccgcaaaat gtctaagaat atcgttgtcc taccgggtga
     3961 tcacgtcggt aaagaagtta ctgacgaagc tattaaggtc ttgaatgcca ttgctgaagt
     4021 ccgtccagaa attaagttca atttccaaca tcacttgatc gggggtgctg ccatcgatgc
     4081 cactggcact cctttaccag atgaagctct agaagcctct aagaaagccg atgctgtctt
     4141 actaggtgct gttggtggtc caaaatgggg tacgggcgca gttagaccag aacaaggtct
     4201 attgaagatc agaaaggaat tgggtctata cgccaacttg agaccatgta actttgcttc
     4261 tgattcttta ctagatcttt ctcctttgaa gcctgaatat gcaaagggta ccgatttcgt
     4321 cgtcgttaga gaattggttg gtggtatcta ctttggtgaa agaaaagaag atgaaggtga
     4381 cggagttgct tgggactctg agaaatacag tgttcctgaa gttcaaagaa ttacaagaat
     4441 ggctgctttc ttggcattgc aacaaaaccc accattacca atctggtctc ttgacaaggc
     4501 taacgtgctt gcctcttcca gattgtggag aaagactgtt gaagaaacca tcaagactga
     4561 gttcccacaa ttaactgttc agcaccaatt gatcgactct gctgctatga ttttggttaa
     4621 atcaccaact aagctaaacg gtgttgttat taccaacaac atgtttggtg atattatctc
     4681 cgatgaagcc tctgttattc caggttcttt gggtttatta ccttctgcat ctctagcttc
     4741 cctacctgac actaacaagg cattcggttt gtacgaacca tgtcatggtt ctgccccaga
     4801 tttaccagca aacaaggtta acccaattgc taccatctta tctgcagcta tgatgttgaa
     4861 gttatccttg gatttggttg aagaaggtag ggctcttgaa gaagctgtta gaaatgtctt
     4921 ggatgcaggt gtcagaaccg gtgaccttgg tggttctaac tctaccactg aggttggcga
     4981 tgctatcgcc aaggctgtca aggaaatctt ggcttaaaga gtcttttgta acgaccccgt
     5041 ctccaccaac ttggtatgct tgaaatctca aggccattac acattcagtt atgtgaacga
     5101 aaggtcttta tttaacgtag cataaactaa ataatacagg ttccggttag cctgcgggcc
     5161 caaagctagc tctaattggt ttaattaata aatttaatat tatttttaaa tttttcttta
     5221 aatatacaat aaatctttca taacatgtta aattcatgat taagcgtaaa taaagtgtag
     5281 tggcagagtg cacggggttt cctgtgcctt acaaagtagg taccaatttg cgtattgcag
     5341 cgagggttcc ggttactatt tataattacg tgttagtgta ctgtgatttt attgaggcta
     5401 taacaagaaa aggatctgtg aaggttttga gagaatgagg cgaaaacaca gggttatttt
     5461 catcagcaac gccgctgttt cagcggcacc ctgccctttt cttttcgcaa gatgagagta
     5521 aagagttgta catcaggtaa gaatgttatt atttaaattc gaagtgataa attcttttca
     5581 tgatgaatca ctcgcttata tggggtagaa tatatatata tgtgtgtgtg tgtgtgtgtt
     5641 tgtgtatgta gggatggtgc gcgtttgttg tgtgacattt gctactcatt cttttccttt
     5701 tcctacgact ggcttaacgg gaatattatc aatttgctgc attcttatgc ttcggtccga
     5761 tgctcaagct taacttcttc gtgaggaagg aaagtgttgt ctcctacttt tttcaaattt
     5821 tcgaattgta tttatattta tttagtactt cttgagttta catatccttc gtaaaaatgc
     5881 aacttttgtc gaaaaacact tccaaaaaaa aataataatg aatttatgaa gcatactaac
     5941 gagcgagcac atcgctgagg tatcattact tcatgagata aattaactgg ccgcacgcca
     6001 cagaaacctc agaataagct aaagggtaat ataataaagc ccttcgattc aataatagca
     6061 ttacccaaga ggcatagcgg caaactaagt tgacaacaca aaatcaaatc gcttcatttt
     6121 tattttatta atgatatttt attagagagg aaattatgta atatctagat attaaatata
     6181 aagtagatct ctgtctaaaa tattcttaac aaaatcttat attcgaaagg atgacggtcc
     6241 aatcaaagga ttgtataaga gaaattagtt ttcgaaatca atttttactt gacctgaact
     6301 taacgatttg ctctcaatcc gcttatcaga aagcaagaac accaacggat cgagcgatca
     6361 cggccccaat caacaacacc aaaaggtata cccatctctc caaatccatg aacttcagct
     6421 ggacctgaga atcgataccc gttccatttg atttgagagg ttttccatat ggtacggaga
     6481 gtgacatatt gggatctgga gctggggatc ggtcaaagga ttcgagaact tggttggcag
     6541 ttaacttggc tccagccaag acgatgggaa ccccagttcc gggatgagtc gaagccccga
     6601 caaagaacaa cttgtcatac ttgggatgtc tggtgctcgg cctgaaacca agaacttgca
     6661 taaagttgtg agccagtccc aagatgcttc cgtccttgag gttaaattct ttctcccaag
     6721 agggagcatc gtgaaccttc tctgccacaa tcattttttc aaagtcggga agtccaagct
     6781 tggcggaaag cgtttggatc acaaacttcc ttgcccgagc aacaagcttg ttgtaatctt
     6841 gagggttcga agcgtcgata tggccacacg gcacaagaat gacgatagca tctttgcctt
     6901 cgggagcggc agaaggatcg attcgcgagg gaacgttcac gtaaaaggaa ggatcggctg
     6961 ggagacccaa ctcctcgaag attgtgtcga atgatccctt gaagtcctcg gccaagaaga
     7021 tattgtgtcc gcccagaccg tccacgattc ggtccatgct ccagtagaag ctcaaactac
     7081 tgcaacttcc cttgagcttc tttccaccaa ctaagtcagc ccaccaactt ctcttgactt
     7141 cacccagttg gccaatcttg tttctggcat cgtcaggaat caagtgctcg gaggcgtaaa
     7201 cgaggtcagc attgacaatc acaacatcgg cgtgatgttc ctcgccggat tcaagtcgaa
     7261 caccagtcgc tcggtccttg gcaggagaga gaagaacctg ggaaactgga gcgttgaaat
     7321 tgaacttggc tgagggattg ttgcgcttga cgatctgaag aagagtatta ggaacctgcc
     7381 aaaagcctcc tctcggatac cagatgccct cggtcaattc ggtgtattgg agcaaggaat
     7441 atgttccggg cgcactgtat gggctttgac ccatgtacat cactgcaaac gagaagactc
     7501 ttcgtaatct gtcggtcttg aaatatcgac aaactcttgt ccagatagac tcgaaggggt
     7561 gaagagccag gatttggcca atgaactgta gccgtaagaa tgctgcgaag ccagggaagt
     7621 tcttctgcag gacgtgaacg acagcaagct cgtaatgtct gtgggcttct tggataaacg
     7681 acaagaaccg atcaaatcca tctttgcctt caaaacgctc gacttcccgc ttgagcaacg
     7741 ccatgtcggt tgaaaaagtg aaagtctctt catcgtggaa gtggcaaaca tagttgggtt
     7801 cacacttgat gagatcgacc caatcttcca tcttctcccc caaatcttcg aatgtctgct
     7861 tgaagagatc tggcaagagc agcaaactgg gcccctgatc gaatcgataa ccatctcgct
     7921 cgattaaaga gcatcgacct ccggagtagt cgttcttctc gaacaccgtg acctggaaac
     7981 cttctttagc aagacgagcg gcagtggcga ttccaccgat accacatccc acgatgatag
     8041 ctgtgggttt atcctgatct tgttcttttc ccattgtaaa tgtgtgtata tattatatta
     8101 tagtattgtt gatattatag ttcttattga tagatgaggg agatgtagat ggaaagagaa
     8161 gaagtggttt ctagcttata ttcagccgaa tggctagagt ttatatatgt ctgcaagccc
     8221 cgtaattcct ccacatttta ctccgcatca catccctcct cagaacaata gttgcctggt
     8281 gcgttgcgca tgtttgaaag tgtacaaaca cttaaccttc cagtaggagc ttaatccaca
     8341 ttatttattt tttagcgtag agaatggctt aactaacctc ggctcggagt ggtcctctcc
     8401 gactcggtgc cctgcagcga tatcatcaca ggcacgcagg cccctgaacg gcacagacca
     8461 cgccgcacgg acgagtgaat gccggtgcct gctggtgccg ccccttccct gttttctggg
     8521 cgggaagtgc tcagggggtg atttcggatg cacgtgacct cgaggcgact ttttggcgac
     8581 aaaaggacta taatcatata ttcggccgtc caaggcccga tccttaatgt gttattgcag
     8641 gatatatgct gcaattcatt ggagttaggt atgaatctgc tggctaaaaa tcgggtgtgc
     8701 ggctgcttgg agtttttcag cctgtgttcc ggcattttgg tgatctggat cttgtaatct
     8761 cctgttctct gtgggctagc gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc
     8821 aaggttttca gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt
     8881 tgaaatataa ataacgttct taatactaac ataactataa aaaaataaat agggacctag
     8941 acttcaggtt gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa
     9001 tgtaagcgtg acataactaa ttacatgact cgaggtcgac ttactgccct tcccatccgc
     9061 tcatgaccac actcaagact ttccgtactc tcctccatcc ggcaactgtc cttctctctc
     9121 cgacgtctcc tttccaaacg actttgatct ctcggccgat cagtaggtag ctcgcgcaag
     9181 ccgctcgcat tcccgcttga acctcggtag gaagtcggtc aattccctta taagaatgtt
     9241 tggcaagatc ctctgcgtag gcgactaatg gaagcgagta cgtcttccat tcgaaccgga
     9301 agctttctga ggcgtttgaa gatggtaatg tggacgaagg agatagactg aggagtttgt
     9361 cgaaatcttg aggccgaggt tccgtccaat cagtcgggat cgcaagcttt gattcatccc
     9421 gaagaccaaa gaatgagagt ggtaggtaaa atctcccttc tgttgcgtcc cctttaatgt
     9481 ccctagcaat gttcaccaac tgaagggcag ttcccatctc tcggcttgcc actaacacag
     9541 cttctctttc ttctatggtg gcagggacct gacttggtgc acttgcccaa gagacataga
     9601 ccaatagctc ggcgactgag cctgctacac atagaccata gtccagcaag tcagctgtgg
     9661 tctcgatagg cgtcttccga gcctggactg cctctgtcga taagggaaag ataagatcag
     9721 tggtgtatcc tctaaggagt tcgtcgagtg ggtatcgagg gatcagccct tgcaacttag
     9781 cgagcaacct gaaggcgaaa tggtattgaa cgggaaccct ttcggtaagg aattgaacga
     9841 gctcggcagg cgagagcgaa ggaggaggcg ggaggggata cattcccgtg ggtcgggaag
     9901 ggtgcgaagg aggaagtaaa ggcgaagaaa ggatcttgtc aggttgcgaa gggtgtagcg
     9961 ggggcccaaa tagtagggta agaaaatcgg agaccatgtc aattgtggca tgcgggttgg
    10021 aagatacttc aggagagtcg ataagatcat cagtcacccg gcagaatgcg tatagtccaa
    10081 ccagcctctc cctaacttcg ctaggaaatc cagccgaggc aacaaaaaag ctccggctct
    10141 ttttctccaa caacttgact gccagttcca agtcacgttt tggctgagaa gagtatggtc
    10201 ggctgctaaa aaacagggag agcacaggcg gtgtaatgag gggaaatgaa gatggcatct
    10261 ttttgttgcc ataaatagtt cgaccgtgta gcaggtatag ggcctgagta tgatcgcagg
    10321 cagacagacc cagaacaatc attagattcg tcagtaagaa gaacatagct tcctcaatgg
    10381 gtagtacacc tccaagcctc caccctacaa tcttctcatc gttgatcgac caagagtctt
    10441 gaccgacagc aacataatct acccaaatca gatacaccgt cgggatcatg attgttgcaa
    10501 tagttgactt tgctcggcca cttttccaat cgaaagcata ttcgcctgat aatgctgcca
    10561 agagcatggt aggtggggtg atgagtaagg agagtgcccg catgtagaag tagtgatctg
    10621 tcacgagcgg gtcgggcgat gggctggggt gagcggtaaa taggtagata atgggcagag
    10681 ggatgagcgc cttgagcgcg agagaaaggg cggacgatct agtcttggga agcgcgagag
    10741 atgggagaag gtgcctagtt gccaagacgt agaccaagcc ggtgattacg gtttgaatga
    10801 caaagaaagc gtacttttca tatggaacat ctagaaacgt tccaaacacg ccttggccac
    10861 tctccgctga tggatatgtc catgcgccat ttctgatgat ccatgagtcc catggtgtgg
    10921 ttgcactaaa cgcaataaat acgaggatcg atattttgta gatgtcaaat tttgtcaaaa
    10981 tcggggaagt gagcaggccg agaagaccaa gaattgggag agtatagatc agatggatct
    11041 ggtaatatgc gagagccgtc attgtaaaaa acttttttta ttatactatt ttctattgtt
    11101 taatagtcgt tgttcttgtg gatatatgaa caagtaatca aaaaagttat cacaaatgaa
    11161 ctcagatata tttcagccga atggctagag tttatatatg tctgtctaac ccacactttt
    11221 tccgcatttt attcctcaac tcatccctcc cttgaacaac gactgctaac aacgtctcac
    11281 tctaaaatct ttacacgttt agctttacag gaggagcata attgactttt gttcctattt
    11341 caagttggga agggcttact ttagcattag ctcggagtgg tccaccccgg aactatggcc
    11401 tgcggaaata ctctcacagg cacatggccc ctgaactgcg gggagtgtgc cgcacggaag
    11461 agtgagcgcc ggtgcctacc ggaagaaccc cttcctcagt atttgagcac gtgactcgaa
    11521 tgctggccgc aaaaaagaaa aaagaatgta atccggttga gccgccccgg aagacgcttt
    11581 tccggtagtt aattctttat acattactgg aacagattct gctccgttta attagacttg
    11641 gatgcaaggt tgcctgtgat agctttagtg ggtctattct acggccttct aatggcaccg
    11701 ttatgacctg gatctggtac gtctgtacga ttagcggggc aagttcaaat aaaattggtt
    11761 taactagtgc gttcaaacca gattttgtat ctgatccaaa gaaaaaacag atgaaactgg
    11821 aggaaatcaa gaaaaaaaca aaagaggcca aaagaagagc aatgaaaaaa aaataaaagc
    11881 tcactgaaaa aaaaaagaga tattgttact attattatta ttactgtcat taatactata
    11941 ctaataaaga aaaggaaaat acgcataata atatttaatt aacaataata taacatatat
    12001 atatatatat atatatgtat gtactaccat gtaatttcaa agactttcaa aagaagttat
    12061 acgtcaattc tcgtgtacaa atcagtcaaa ctgtaattca ttcgtactga taagccaaat
    12121 ttctacctag tattcatatg aagatttgaa aaactatgat aaaaaacgaa caatatcatt
    12181 actatttgct tctcttgtaa actttgttca aaaacgcagt taagacgtcg gctttgaagc
    12241 cacgagactc gtcgacttgg gagatcttgg ccttcaaatc cttggcaaca gactcttcat
    12301 attcgtggta taactggtcg attttcaagt cattgaaaat ctttttgcat ttggcttctg
    12361 cgactgagtc cttcttaccg taattttcgt ctaaagtctt tctttgttct gcggaagcaa
    12421 gttctaatgc cttgttgatt acccaagaac atttgttatc ttggatatct gtaccgatct
    12481 taccgatctg ttctggggta ccgaagcagt ctaagtagtc atcttgaatt tggaaatatt
    12541 cacccaatgg aatcaagaca tctctggctt gtttcaaatc cttttcatct gtgataccgg
    12601 caacgtacat agccaatgcg acaggcaagt agaaagaata gtaagcagtc ttgaaagtaa
    12661 ctatgaagga gtgcttcttt agggagaact tactcaagtc gactttgtct tcaggtgcag
    12721 tgattaagtc catcaattgg cccaattcgg tttggaaggt gacttcatgg aacaattcgg
    12781 tgatatctat gtagtatttt tcgtttctga agtgagattt caaaagcttg tagatagcag
    12841 cctctaacat gaatgcgtca ttgatggcaa tttccccaac ttcaggaacc ttgtaccaac
    12901 atggttggcc tcttctggta atggacttgt ccatcatatc atcggcgacc aaacagtaag
    12961 cctgcaacaa ctcaatgcac caacctagaa tagcaacctt ttcgtattct tcttgcccca
    13021 attgttcaac ggtcttgttg gagagaatag catacgtgtc cacaacggac aaacctctat
    13081 ttaacttacc gcctggagtg ttgtagttca atgagtgggc ataccagtca catgcttcct
    13141 taggcatacc gtaagccaaa agcgatgcgt tcaattcctc tactaattta gggaaaacgt
    13201 tcaagaatct ctctctccta atttcttttt ctgaagccat tttgctagct atagtttttt
    13261 ctccttgacg ttaaagtata gaggtatatt aacaattttt tgttgatact tttatgacat
    13321 ttgaataaga agtaatacaa accgaaaatg ttgaaagtat tagttaaagt ggttatgcag
    13381 cttttgcatt tatatatctg ttaatagatc aaaaatcatc gcttcgctga ttaattaccc
    13441 cagaaataag gctaaaaaac taatcgcatt attatcctat ggttgttaat ttgattcgtt
    13501 gatttgaagg tttgtggggc caggttactg ccaatttttc ctcttcataa ccataaaagc
    13561 tagtattgta gaatctttat tgttcggagc agtgcggcgc gaggcacatc tgcgtttcag
    13621 gaacgcgacc ggtgaagacc aggacgcacg gaggagagtc ttccgtcgga gggctgtcgc
    13681 ccgctcggcg gcttctaatc cgtacttcaa tatagcaatg agcagttaag cgtattactg
    13741 aaagttccaa agagaaggtt tttttaggct aagataatgg ggctctttac atttccacaa
    13801 catataagta agattagata tggatatgta tatggtggta ttgccatgta atatgattat
    13861 taaacttctt tgcgtccatc caaaaaaaaa gtaagaattt ttgaaaattc aatataagga
    13921 tcccccggga atctcggtcg taatgatttg catgcccgcc atctct
//
