LOCUS       sequence-370866-       10806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10806)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPAeBlueHPV16LR
FEATURES             Location/Qualifiers
     source          1..10806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1256..1526
                     /gene="S. cerevisae PGK1"
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate 
                     kinase gene"
     CDS             3720..3737
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4100..4120)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4100..4118)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4139..4155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4139..4155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4152..4174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4163..4179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4187..4193,4194..4211,4212..4217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4232..4253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4370..4387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4541..5129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4621..4640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5300..6091,6092..6160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5923..5942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6161..6265)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6292..7634
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7255..7302)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7675..7693
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7731..7753)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7853..7872
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(8366..9460)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(9461..9868)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(10169..10624)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10306..10327)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10518..10537
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10750..10772
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10764..10781
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10765..10781
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10791..10806,1..4)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(10791..10806,1..3)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agggcgaatt gggtaccggg ccccatatgg cgtatccaag ccgaaacggc gctcgcctca
       61 tccccacggg aataaggcag ccgacaaaag aaaaacgacc gaaaaggaac cagaaagaaa
      121 aaagagggtg ggcgcgccgc ggacgtgtaa aaagatatgc atccagcttc tatatcgctt
      181 taactttacc gttttgggca tcgggaacgt atgtaacatt gatctcctct tgggaacggt
      241 gagtgcaacg aatgcgatat agcaccgacc atgtgggcaa attcgtaata aattcggggt
      301 gagggggatt caagacaagc aaccttgtta gtcagctcaa acagcgattt aacggttgag
      361 taacacatca aaacaccgtt cgaggtcaag cctggcgtgt ttaacaagtt cttgatatca
      421 tatataaatg taataagaag tttggtaata ttcaattcga agtgttcagt cttttacttc
      481 tcttgtttta tagaagaaaa aacatcaaga aacatcttta acatacacaa acacatacta
      541 tcagaataca ggatccaaaa tggcttcttt ggttaagaaa gatatgtgta tcaagatgac
      601 tatggaaggt actgttaacg gtcatcactt taagtgtgtt ggtgaaggtg aaggtaaacc
      661 atttgaaggt actcaagttg aaaagattag aattactgaa ggtggtccat tgccatttgc
      721 ttatgatatt ttggctccat gttgtatgta tggttctaag actttcatta agcacgtttc
      781 tggtatccca gactacttca aagaatcttt tccagaaggt tttacctggg aaagaactca
      841 aatctttgaa gatggtggtt acttgactat tcaccaagat acttcattgc aaggtaacaa
      901 ctttatcttc aaagttaacg ttatcggtgc taattttcca gctaatggtc cagttatgca
      961 aaagaaaact gctggttggg aaccatgtgt tgaaatgttg tatccaagag atggtgtttt
     1021 gtgcggtcaa tctttgatgg ctttgaaatg tactgatggt aatcacttga cctctcattt
     1081 gagaactact tacagatcca gaaaaccatc taacgctgtt aacatgccag aatttcattt
     1141 cggtgaccac agaattgaga ttttgaaagc tgaacaaggt aagttctacg aacaatacga
     1201 atctgctgtt gctagatatt gtgaagctgc tccatcaaaa ttgggtcatc attaaattga
     1261 attgaattga aatcgataga tcaatttttt tcttttctct ttccccatcc tttacgctaa
     1321 aataatagtt tattttattt tttgaatatt ttttatttat atacgtatat atagactatt
     1381 atttatcttt taatgattat taagattttt attaaaaaaa aattcgctcc tcttttaatg
     1441 cctttatgca gttttttttt cccattcgat atttctatgt tcgggttcag cgtattttaa
     1501 gtttaataac tcgaaaattc tgcgttcgtt aaagctttcg agaaggatat tatttcccgg
     1561 gccacagaga acaggagatt acaagatcca gatcaccaaa atgccggaac acaggctgaa
     1621 aaactccaag cagccgcaca cccgattttt agccagcaga ttcataccta actccaatga
     1681 attgcagcat atatcctgca ataacacatt aaggatcggg ccttggacgg ccgaatatat
     1741 gattatagtc cttttgtcgc caaaaagtcg cctcgaggtc acgtgcatcc gaaatcaccc
     1801 cctgagcact tcccgcccag aaaacaggga aggggcggca ccagcaggca ccggcattca
     1861 ctcgtccgtg cggcgtggtc tgtgccgttc aggggcctgc gtgcctgtga tgatatcgct
     1921 gcagggcacc gagtcggaga ggaccactcc gagccgaggt tagttaagcc attctctacg
     1981 ctaaaaaata aataatgtgg attaagctcc tactggaagg ttaagtgttt gtacactttc
     2041 aaacatgcgc aacgcaccag gcaactattg ttctgaggag ggatgtgatg cggagtaaaa
     2101 tgtggaggaa ttacggggct tgcagacata tataaactct agccattcgg ctgaatataa
     2161 gctagaaacc acttcttctc tttccatcta catctccctc atctatcaat aagaactata
     2221 atatcaacaa tactataata taatatatac acacatttac agtcgacaaa atgtctttgt
     2281 ggttgccatc tgaagctact gtttacttgc caccagttcc agtttctaag gttgtttcta
     2341 ctgatgaata cgtcgccaga actaacatct attatcatgc tggtacttcc aggttgttgg
     2401 ctgttggtca tccatatttt ccaatcaaga agcccaacaa caacaagatt ttagttccaa
     2461 aagtttccgg tttacagtac agagttttca gaatccattt gccagatcca aacaagtttg
     2521 gtttcccaga tacttcattc tacaacccag atacacaaag attggtttgg gcttgtgttg
     2581 gtgttgaagt tggtagaggt caaccattag gtgttggtat ttctggtcat cctttgttga
     2641 acaagttgga cgatactgaa aatgcttctg cttatgctgc taatgctggt gttgataaca
     2701 gagaatgcat ctctatggat tacaagcaaa cccagttgtg tttgattggt tgtaaaccac
     2761 ctattggtga acattggggt aaaggttctc catgtactaa tgttgctgtt aatccaggtg
     2821 actgtccacc attggaattg attaacaccg ttattcaaga tggcgatatg gttgatactg
     2881 gttttggtgc tatggatttc actacattgc aagccaacaa atccgaagtt ccattggata
     2941 tctgtacctc tatctgtaag tacccagact acatcaagat ggttagtgaa ccttatggtg
     3001 actccttgtt cttctacttg agaagagaac aaatgttcgt caggcatttg tttaatagag
     3061 ctggtgctgt tggtgaaaat gttccagatg acttgtacat caaaggttct ggttctactg
     3121 ctaacttggc ttcttctaat tacttcccaa ctccatctgg ttcaatggtt acttctgatg
     3181 cccaaatttt caacaagcca tattggttgc aaagagccca aggtcataac aatggtattt
     3241 gttggggtaa ccagttgttc gttactgttg ttgataccac cagatccact aacatgtctt
     3301 tatgtgctgc tatctctact tccgaaacta cttacaagaa cacgaacttc aaagaatact
     3361 tgaggcacgg tgaagaatac gacttgcaat tcatttttca gctgtgcaag attaccttga
     3421 ccgctgatgt tatgacttac atccattcta tgaactccac catcttggaa gattggaatt
     3481 ttggtctaca accaccacca ggtggtactt tggaagatac ctatagattc gttacctctc
     3541 aagctatcgc ttgtcaaaaa catactccac cagctccaaa agaagatcca ttgaagaaat
     3601 acactttctg ggaagtcaac ctgaaagaaa agttctctgc tgatttggat caattcccat
     3661 tgggtagaaa gttcttgttg caagctggtt tgaaagctaa gccaaagttc actttgggtc
     3721 accaccatca ccatcattaa tagtgacctg cagggcggat tgagagcaaa tcgttaagtt
     3781 caggtcaagt aaaaattgat ttcgaaaact aatttctctt atacaatcct ttgattggac
     3841 cgtcatcctt tcgaatataa gattttgtta agaatatttt agacagagat ctactttata
     3901 tttaatatct agatattaca taatttcctc tctaataaaa tatcattaat aaaataaaaa
     3961 tgaagcgatt tgattttgtg ttgtcaactt agtttgccgc tatgcctctt gggtaatgct
     4021 attattgaat cgaagggctt tattatatta ccctttagct tattctgagg tttctgtggc
     4081 ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4981 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     5941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca
     6301 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     6361 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     6421 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa
     6481 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     6541 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     6601 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     6661 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     6721 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     6781 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     6841 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     6901 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     6961 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     7021 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     7081 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     7141 gcaatgtttg tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt
     7201 tttggttttt tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt
     7261 cctatacttt ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg
     7321 agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct
     7381 atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat
     7441 gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc
     7501 tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc
     7561 tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc
     7621 tttgatattg gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg
     7681 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7741 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7801 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     7861 gagcagattg tactgagagt gcaccatatc gactacgtcg ttaaggccgt ttctgacaga
     7921 gtaaaattct tgagggaact ttcaccatta tgggaaatgg ttcaagaagg tattgactta
     7981 aactccatca aatggtcagg tcattgagtg ttttttattt gttgtatttt ttttttttta
     8041 gagaaaatcc tccaatatat aaattaggaa tcatagtttc atgattttct gttacaccta
     8101 actttttgtg tggtgccctc ctccttgtca atattaatgt taaagtgcaa ttctttttcc
     8161 ttatcacgtt gagccattag tatcaatttg cttacctgta ttcctttaca tcctcctttt
     8221 tctccttctt gataaatgta tgtagattgc gtatatagtt tcgtctaccc tatgaacata
     8281 ttccattttg taatttcgtg tcgtttctat tatgaatttc atttataaag tttatgtaca
     8341 aatatcataa aaaaagagaa tctttttaag caaggatttt cttaacttct tcggcgacag
     8401 catcaccgac ttcggtggta ctgttggaac cacctaaatc accagttctg atacctgcat
     8461 ccaaaacctt tttaactgca tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca
     8521 atttcaacat cattgcagca gacaagatag tggcgatagg gttgacctta ttctttggca
     8581 aatctggagc agaaccgtgg catggttcgt acaaaccaaa tgcggtgttc ttgtctggca
     8641 aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc tgggataacg gaggcttcat
     8701 cggagatgat atcaccaaac atgttgctgg tgattataat accatttagg tgggttgggt
     8761 tcttaactag gatcatggcg gcagaatcaa tcaattgatg ttgaaccttc aatgtaggga
     8821 attcgttctt gatggtttcc tccacagttt ttctccataa tcttgaagag gccaaaacat
     8881 tagctttatc caaggaccaa ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg
     8941 ccattcttgt gattctttgc acttctggaa cggtgtattg ttcactatcc caagcgacac
     9001 catcaccatc gtcttccttt ctcttaccaa agtaaatacc tcccactaat tctctgacaa
     9061 caacgaagtc agtaccttta gcaaattgtg gcttgattgg agataagtct aaaagagagt
     9121 cggatgcaaa gttacatggt cttaagttgg cgtacaattg aagttcttta cggattttta
     9181 gtaaaccttg ttcaggtcta acactaccgg taccccattt aggaccaccc acagcaccta
     9241 acaaaacggc atcagccttc ttggaggctt ccagcgcctc atctggaagt ggaacacctg
     9301 tagcatcgat agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac
     9361 gaacatcaga aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt
     9421 ggtcacctgg caaaacgacg atcttcttag gggcagacat tagaatggta tatccttgaa
     9481 atatatatat atatattgct gaaatgtaaa aggtaagaaa agttagaaag taagacgatt
     9541 gctaaccacc tattggaaaa aacaataggt ccttaaataa tattgtcaac ttcaagtatt
     9601 gtgatgcaag catttagtca tgaacgcttc tctattctat atgaaaagcc ggttccggcg
     9661 ctctcacctt tcctttttct cccaattttt cagttgaaaa aggtatatgc gtcaggcgac
     9721 ctctgaaatt aacaaaaaat ttccagtcat cgaatttgat tctgtgcgat agcgcccctg
     9781 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
     9841 cttacgatac ctgagtattc ccacagttaa ctgcggtcaa gatatttctt gaatcaggcg
     9901 ccttagaccg ctcggccaaa caaccaatta cttgttgaga aatagagtat aattatccta
     9961 taaatataac gtttttgaac acacatgaac aaggaagtac aggacaattg attttgaaga
    10021 gaatgtggat tttgatgtaa ttgttgggat tccattttta ataaggcaat aatattaggt
    10081 atgtagatat actagaagtt ctcctcgacc ggtcgatatg cggtgtgaaa taccgcacag
    10141 atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt gttaaaattc
    10201 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
    10261 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
    10321 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
    10381 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
    10441 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
    10501 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
    10561 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
    10621 gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    10681 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    10741 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
    10801 cactat
//
