LOCUS       sequence-37089-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: pCMV:ECFP(FRT)EYFP
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     107..128
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(200..233)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(259..278)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     313..332
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(528..549)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggcaggcngg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
       61 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      121 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg
      181 atggatctcc taggcccggg aagttcctat actttctaga gaataggaac ttcggatcca
      241 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
      301 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      361 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      421 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta
      481 gattacttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc
      541 aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg
      601 tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg
      661 tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg
      721 ccgttcttct gcttgtcggc ggtgatatag acgttgtggc tgatgtagtt gtactccagc
      781 ttgtgcccca ggatgttgcc
//
