LOCUS       sequence-370907-       10085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sTALED for plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Mok YG, Hong S, Bae SJ, Cho SI, Kim JS
  TITLE     Targeted A-to-G base editing of chloroplast DNA in plants.
  JOURNAL   Nat Plants. 2022 Dec 1. doi: 10.1038/s41477-022-01279-8.
  PUBMED    36456803
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2022 Dec 1. doi: 10.1038/s41477-022-01279-8."
COMMENT     Sequence Label: AatII-pRPS5A-PTP-3Xflag
FEATURES             Location/Qualifiers
     source          1..10085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(5..25)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             1857..1922
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2272..2296
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3627..4256
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4685..5758
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5824..6018
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6254..6276)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6362..6502
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6417..6436
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6517..6534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6688..7276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6768..6787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7522..8313)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8834..8858
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8951..9203)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9223..9774)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(9816..9995)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgttcaactt ttctatacaa agttgagacg tcgactcaac ttttgattcg ctatttgcag
       61 tgcacctgtg gcgttcatca catcttttgt gacactgttt gcactggtca ttgctattac
      121 aaaggacctt cctgatgttg aaggagatcg aaagtaagta actgcacgca taaccatttt
      181 ctttccgctc tttggctcaa tccatttgac agtcaaagac aatgtttaac cagctccgtt
      241 tgatatattg tctttatgtg tttgttcaag catgtttagt taatcatgcc tttgattgat
      301 cttgaatagg ttccaaatat caaccctggc aacaaaactt ggagtgagaa acattgcatt
      361 cctcggttct ggacttctgc tagtaaatta tgtttcagcc atatcactag ctttctacat
      421 gcctcaggtg aattcatcta tttccgtctt aactatttcg gttaattaaa gcacgaacac
      481 cattactgca tgtagaagct tgataaacta tcgccaccaa tttatttttg ttgcgatatt
      541 gttactttcc tcagtatgca gctttgaaaa gaccaaccct cttatccttt aacaatgaac
      601 aggtttttag aggtagcttg atgattcctg cacatgtgat cttggcttca ggcttaattt
      661 tccaggtaaa gcattatgag atactcttat atctcttaca tacttttgag ataatgcaca
      721 agaacttcat aactatatgc tttagtttct gcatttgaca ctgccaaatt cattaatctc
      781 taatatcttt gttgttgatc tttggtagac atgggtacta gaaaaagcaa actacaccaa
      841 ggtaaaatac ttttgtacaa acataaactc gttatcacgg aacatcaatg gagtgtatat
      901 ctaacggagt gtagaaacat ttgattattg caggaagcta tctcaggata ttatcggttt
      961 atatggaatc tcttctacgc agagtatctg ttattcccct tcctctagct ttcaatttca
     1021 tggtgaggat atgcagtttt ctttgtatat cattcttctt cttctttgta gcttggagtc
     1081 aaaatcggtt ccttcatgta catacatcaa ggatatgtcc ttctgaattt ttatatcttg
     1141 caataaaaat gcttgtacca attgaaacac cagctttttg agttctatga tcactgactt
     1201 ggttctaacc aaaaaaaaaa aaatgtttaa tttacatatc taaaagtagg tttagggaaa
     1261 cctaaacagt aaaatatttg tatattattc gaatttcact catcataaaa acttaaattg
     1321 caccataaaa ttttgtttta ctattaatga tgtaatttgt gtaacttaag ataaaaataa
     1381 tattccgtaa gttaaccggc taaaaccacg tataaaccag ggaacctgtt aaaccggttc
     1441 tttactggat aaagaaatga aagcccatgt agacagctcc attagagccc aaaccctaaa
     1501 tttctcatct atataaaagg agtgacatta gggtttttgt tcgtcctctt aaagcttctc
     1561 gttttctctg ccgtctctct cattcgcgcg acgcaaacga tcttcaggtg atcttctttc
     1621 tccaaatcct ctctcataac tctgatttcg tacttgtgta tttgagctca cgctctgttt
     1681 ctctcaccac agccatggat tcacagctag tcttgtctct gaagctgaat ccaagcttca
     1741 ctcctctttc tcctctcttc cctttcactc catgttcttc tttttcgccg tcgctccggt
     1801 tttcttcttg ctactcccgc cgcctctatt ctccggttac cgtctacgcc gcgaaggact
     1861 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     1921 agcccgggtc aggatcctct ggtacctgat aatcgatatc ccgcggccat gctagagtcc
     1981 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     2041 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     2101 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     2161 taattcctaa aaccaaaatc cagtgacctg caggcatgcc gtcgggccca agcttagctt
     2221 gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat
     2281 atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag
     2341 ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc
     2401 tcgggatcaa agtactttaa agtactttaa agtactttaa agtactttga tccaacccct
     2461 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
     2521 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
     2581 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
     2641 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
     2701 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
     2761 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
     2821 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
     2881 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
     2941 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
     3001 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
     3061 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
     3121 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
     3181 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
     3241 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
     3301 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
     3361 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
     3421 gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
     3481 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
     3541 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
     3601 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
     3661 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
     3721 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
     3781 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
     3841 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
     3901 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
     3961 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
     4021 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
     4081 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
     4141 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
     4201 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
     4261 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
     4321 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
     4381 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
     4441 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
     4501 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
     4561 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
     4621 taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat
     4681 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     4741 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     4801 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     4861 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     4921 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     4981 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     5041 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     5101 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     5161 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     5221 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     5281 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     5341 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     5401 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     5461 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     5521 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     5581 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     5641 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     5701 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     5761 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     5821 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     5881 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     5941 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     6001 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     6061 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     6121 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     6181 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     6241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     6301 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     6361 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     6421 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6481 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6541 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6601 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6661 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6721 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6781 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6841 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6901 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6961 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7021 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7081 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     7141 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7201 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7261 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7321 cgaaaactca cgttaaggga ttttggtcat gcatgatata tctcccaatt tgtgtagggc
     7381 ttattatgca cgcttaaaaa taataaaagc agacttgacc tgatagtttg gctgtgagca
     7441 attatgtgct tagtgcatct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt
     7501 gaacgaattt ctagctagac attatttgcc gactaccttg gtgatctcgc ctttcacgta
     7561 gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag
     7621 ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc
     7681 ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg
     7741 ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg agttccatag
     7801 cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc
     7861 ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc
     7921 cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca
     7981 ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac
     8041 aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc
     8101 caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac
     8161 cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg
     8221 tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg
     8281 agtcgatact tcggcgatca ccgcttcccc catgatgttt aactttgttt tagggcgact
     8341 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     8401 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
     8461 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     8521 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     8581 ggttcgtgcc cgaattgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct
     8641 accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata
     8701 gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc
     8761 ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct
     8821 gttggctggc tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta
     8881 ataacacatt gcggacgttt ttaatgtact gaattaacgc cgaattgaat tatcagcttg
     8941 catgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     9001 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     9061 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     9121 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     9181 tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt cggtgacggg
     9241 caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac ccacgtcatg
     9301 ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat atccgagcgc
     9361 ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca cgctcttgaa
     9421 gccctgtgcc tccagggact tcagaaggtg ggtgtagagc gtggagccca gtcccgtccg
     9481 ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg cgttgcgtgc
     9541 cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg cgacgagcca
     9601 gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt cctgcggctc
     9661 ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc agaccgccgg
     9721 catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc tcatggtaga
     9781 tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     9841 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     9901 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     9961 cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
    10021 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatcga
    10081 taatt
//
